WO2005113820A3 - Methods and devices related to a regulated flow electroporation chamber - Google Patents

Methods and devices related to a regulated flow electroporation chamber Download PDF

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Publication number
WO2005113820A3
WO2005113820A3 PCT/US2005/016728 US2005016728W WO2005113820A3 WO 2005113820 A3 WO2005113820 A3 WO 2005113820A3 US 2005016728 W US2005016728 W US 2005016728W WO 2005113820 A3 WO2005113820 A3 WO 2005113820A3
Authority
WO
WIPO (PCT)
Prior art keywords
electroporation chamber
regulated flow
methods
flow electroporation
devices related
Prior art date
Application number
PCT/US2005/016728
Other languages
French (fr)
Other versions
WO2005113820A2 (en
Inventor
Sergey Dzekunov
Nicholas Chopas
Original Assignee
Maxcyte Inc
Sergey Dzekunov
Nicholas Chopas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxcyte Inc, Sergey Dzekunov, Nicholas Chopas filed Critical Maxcyte Inc
Priority to KR1020067026146A priority Critical patent/KR101301929B1/en
Priority to CN2005800152624A priority patent/CN101426929B/en
Priority to JP2007513390A priority patent/JP4937904B2/en
Priority to CA2565316A priority patent/CA2565316C/en
Priority to AU2005245871A priority patent/AU2005245871A1/en
Priority to EP05748359.6A priority patent/EP1766057B1/en
Priority to DK05748359.6T priority patent/DK1766057T3/en
Publication of WO2005113820A2 publication Critical patent/WO2005113820A2/en
Priority to IL179084A priority patent/IL179084A0/en
Publication of WO2005113820A3 publication Critical patent/WO2005113820A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip

Abstract

The electroporation chamber and its related devices combine the features of an electroporation chamber that acts as a manifold for regulation of sample flow with those of a flow electroporation device to form a regulated flow electroporation device. The invention further comprises a novel regulated flow electroporation chamber that enables conditions in which a sample is uniformly processed in individual fractions or volumes in a fully closed (sterile) system.
PCT/US2005/016728 2004-05-12 2005-05-12 Methods and devices related to a regulated flow electroporation chamber WO2005113820A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
KR1020067026146A KR101301929B1 (en) 2004-05-12 2005-05-12 Methods and Devices Related to a Regulated Flow Electroporation Chamber
CN2005800152624A CN101426929B (en) 2004-05-12 2005-05-12 Methods and devices related to a regulated flow electroporation chamber
JP2007513390A JP4937904B2 (en) 2004-05-12 2005-05-12 Method and apparatus associated with a controlled flow electroporation chamber
CA2565316A CA2565316C (en) 2004-05-12 2005-05-12 Methods and devices related to a regulated flow electroporation chamber
AU2005245871A AU2005245871A1 (en) 2004-05-12 2005-05-12 Methods and devices related to a regulated flow electroporation chamber
EP05748359.6A EP1766057B1 (en) 2004-05-12 2005-05-12 Methods and devices related to a regulated flow electroporation chamber
DK05748359.6T DK1766057T3 (en) 2004-05-12 2005-05-12 Methods and devices related to a regulated flowelektroporationskammer
IL179084A IL179084A0 (en) 2004-05-12 2006-11-06 Methods and devices related to a regulated flow electroporation chamber

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57031704P 2004-05-12 2004-05-12
US60/570,317 2004-05-12

Publications (2)

Publication Number Publication Date
WO2005113820A2 WO2005113820A2 (en) 2005-12-01
WO2005113820A3 true WO2005113820A3 (en) 2009-04-02

Family

ID=35428944

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/016728 WO2005113820A2 (en) 2004-05-12 2005-05-12 Methods and devices related to a regulated flow electroporation chamber

Country Status (10)

Country Link
US (2) US7771984B2 (en)
EP (1) EP1766057B1 (en)
JP (1) JP4937904B2 (en)
KR (1) KR101301929B1 (en)
CN (2) CN101426929B (en)
AU (1) AU2005245871A1 (en)
CA (1) CA2565316C (en)
DK (1) DK1766057T3 (en)
IL (1) IL179084A0 (en)
WO (1) WO2005113820A2 (en)

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US20050282200A1 (en) 2005-12-22
US7771984B2 (en) 2010-08-10
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WO2005113820A2 (en) 2005-12-01
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CA2565316A1 (en) 2005-12-01
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