WO2005111230A3 - Apoenzyme reactivation electrochemical detection method and assay - Google Patents

Apoenzyme reactivation electrochemical detection method and assay Download PDF

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Publication number
WO2005111230A3
WO2005111230A3 PCT/US2005/006290 US2005006290W WO2005111230A3 WO 2005111230 A3 WO2005111230 A3 WO 2005111230A3 US 2005006290 W US2005006290 W US 2005006290W WO 2005111230 A3 WO2005111230 A3 WO 2005111230A3
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WO
WIPO (PCT)
Prior art keywords
dry reagent
apoenzyme
assay
detection method
electrochemical detection
Prior art date
Application number
PCT/US2005/006290
Other languages
French (fr)
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WO2005111230A2 (en
Inventor
Stephen Eliot Zweig
Original Assignee
Stephen Eliot Zweig
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Publication date
Application filed by Stephen Eliot Zweig filed Critical Stephen Eliot Zweig
Publication of WO2005111230A2 publication Critical patent/WO2005111230A2/en
Publication of WO2005111230A3 publication Critical patent/WO2005111230A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/004—Enzyme electrodes mediator-assisted
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a method in which dry reagent enzyme based electrochemical biosensors, which are in a relatively mature form due to the extensive amount of development pioneered by the blood glucose monitoring industry, may be simply adapted to perform tests for blood coagulation, enzymatic activity, or immunochemical assays for antigens present in a fluid sample. In particular, the utility of combining apoenzyme based dry reagent electrochemical biosensors with apoenzyme reactivation technology is taught. This combination creates a novel combination dry reagent test technology capable of detecting a wide range of different analytes.
PCT/US2005/006290 2004-03-03 2005-03-01 Apoenzyme reactivation electrochemical detection method and assay WO2005111230A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US54969104P 2004-03-03 2004-03-03
US60/549,691 2004-03-03
US11/059,841 US7166208B2 (en) 2004-03-03 2005-02-17 Apoenzyme reactivation electrochemical detection method and assay
US11/059,841 2005-02-17

Publications (2)

Publication Number Publication Date
WO2005111230A2 WO2005111230A2 (en) 2005-11-24
WO2005111230A3 true WO2005111230A3 (en) 2007-02-01

Family

ID=34914879

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/006290 WO2005111230A2 (en) 2004-03-03 2005-03-01 Apoenzyme reactivation electrochemical detection method and assay

Country Status (2)

Country Link
US (1) US7166208B2 (en)
WO (1) WO2005111230A2 (en)

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Also Published As

Publication number Publication date
US20050196820A1 (en) 2005-09-08
WO2005111230A2 (en) 2005-11-24
US7166208B2 (en) 2007-01-23

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