WO2005098050A3 - Base specific cleavage of methylation-specific amplification products in combination with mass analysis - Google Patents

Base specific cleavage of methylation-specific amplification products in combination with mass analysis Download PDF

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Publication number
WO2005098050A3
WO2005098050A3 PCT/US2005/009929 US2005009929W WO2005098050A3 WO 2005098050 A3 WO2005098050 A3 WO 2005098050A3 US 2005009929 W US2005009929 W US 2005009929W WO 2005098050 A3 WO2005098050 A3 WO 2005098050A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecule
target nucleic
methylation
methylation state
Prior art date
Application number
PCT/US2005/009929
Other languages
French (fr)
Other versions
WO2005098050A2 (en
Inventor
Mathias Ehrich
Den Boom Dirk Johannes Van
Original Assignee
Sequenom Inc
Mathias Ehrich
Den Boom Dirk Johannes Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sequenom Inc, Mathias Ehrich, Den Boom Dirk Johannes Van filed Critical Sequenom Inc
Priority to CA2561381A priority Critical patent/CA2561381C/en
Priority to AU2005230936A priority patent/AU2005230936B2/en
Priority to EP05731507A priority patent/EP1727911B1/en
Publication of WO2005098050A2 publication Critical patent/WO2005098050A2/en
Priority to HK07105712.9A priority patent/HK1098792A1/en
Publication of WO2005098050A3 publication Critical patent/WO2005098050A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

Methods, combinations and kits are provided for identifying the methylation state of a target nucleic acid molecule, the methylate state of a nucleotide locus in a target nucleic acid molecule, or for identifying the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule. Methylation state identification is performed by treating a methylated target nucleic acid molecule with a reagent that modifies one or more nucleotides in the target nucleic acid molecule as a function of the methylation state of the target nucleic acid molecule, methylation specifically amplifying treated target nucleic acid molecule, fragmenting amplified products, and detecting one or more fragments to thereby identify the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule.
PCT/US2005/009929 2004-03-26 2005-03-24 Base specific cleavage of methylation-specific amplification products in combination with mass analysis WO2005098050A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2561381A CA2561381C (en) 2004-03-26 2005-03-24 Base specific cleavage of methylation-specific amplification products in combination with mass analysis
AU2005230936A AU2005230936B2 (en) 2004-03-26 2005-03-24 Base specific cleavage of methylation-specific amplification products in combination with mass analysis
EP05731507A EP1727911B1 (en) 2004-03-26 2005-03-24 Base specific cleavage of methylation-specific amplification products in combination with mass analysis
HK07105712.9A HK1098792A1 (en) 2004-03-26 2007-05-29 Base specific cleavage of methylation-specific amplification products in combination with mass analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55663204P 2004-03-26 2004-03-26
US60/556,632 2004-03-26

Publications (2)

Publication Number Publication Date
WO2005098050A2 WO2005098050A2 (en) 2005-10-20
WO2005098050A3 true WO2005098050A3 (en) 2007-11-08

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/009929 WO2005098050A2 (en) 2004-03-26 2005-03-24 Base specific cleavage of methylation-specific amplification products in combination with mass analysis

Country Status (7)

Country Link
US (1) US9249456B2 (en)
EP (2) EP1727911B1 (en)
CN (1) CN101233240A (en)
AU (1) AU2005230936B2 (en)
CA (1) CA2561381C (en)
HK (1) HK1098792A1 (en)
WO (1) WO2005098050A2 (en)

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