WO2005084404A3 - Microfluidic biosensor and methods of use - Google Patents

Microfluidic biosensor and methods of use Download PDF

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Publication number
WO2005084404A3
WO2005084404A3 PCT/US2005/007378 US2005007378W WO2005084404A3 WO 2005084404 A3 WO2005084404 A3 WO 2005084404A3 US 2005007378 W US2005007378 W US 2005007378W WO 2005084404 A3 WO2005084404 A3 WO 2005084404A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
marker
capture
conjugate
binding material
Prior art date
Application number
PCT/US2005/007378
Other languages
French (fr)
Other versions
WO2005084404A2 (en
Inventor
Antje J Baeumner
Sylvia Dokua Sakyiama Kwakye
Vasiliy N Goral
Natalya V Zaytseva
Original Assignee
Cornell Res Foundation Inc
Antje J Baeumner
Sylvia Dokua Sakyiama Kwakye
Vasiliy N Goral
Natalya V Zaytseva
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Res Foundation Inc, Antje J Baeumner, Sylvia Dokua Sakyiama Kwakye, Vasiliy N Goral, Natalya V Zaytseva filed Critical Cornell Res Foundation Inc
Publication of WO2005084404A2 publication Critical patent/WO2005084404A2/en
Publication of WO2005084404A3 publication Critical patent/WO2005084404A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Fluid Mechanics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a method for detecting or quantifying an analyte in a test sample. This involves providing at least one test mixture where the test mixture includes a test sample, potentially containing analyte, a capture conjugate, and a marker conjugate. The capture conjugate includes a capture support and a first binding material, where the first binding material is selected to bind with a portion of the analyte. The marker conjugate includes a particle, a marker, and a second binding material, where the second binding material is selected to bind with a portion of the analyte other than the portion of the analyte for which the first binding material is selected. Reaction is permitted to occur in the test mixture between analyte present in the test sample and the first and second binding materials, thereby forming a product complex comprising analyte present in the test sample, the capture conjugate, and the marker conjugate. The reacted test mixture is exposed to a capture device having non-specific affinity for the capture support, whereby product complex present in the reacted test mixture is immobilized from the reacted test mixture. The presence or amount of the marker from the immobilized product complex is detected using a detection assembly. The presence or amount of the marker from the immobilized product complex is correlated with the presence or amount, respectively, of the analyte in the test sample. A device for carrying out this method and similar methods with other assay formats are also disclosed.
PCT/US2005/007378 2004-03-03 2005-03-03 Microfluidic biosensor and methods of use WO2005084404A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54972904P 2004-03-03 2004-03-03
US60/549,729 2004-03-03

Publications (2)

Publication Number Publication Date
WO2005084404A2 WO2005084404A2 (en) 2005-09-15
WO2005084404A3 true WO2005084404A3 (en) 2005-12-01

Family

ID=34919532

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/007378 WO2005084404A2 (en) 2004-03-03 2005-03-03 Microfluidic biosensor and methods of use

Country Status (1)

Country Link
WO (1) WO2005084404A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003243348B2 (en) * 2002-05-31 2009-12-03 Cornell Research Foundation, Inc. Universal biosensor and methods of use

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4372745A (en) * 1979-12-19 1983-02-08 Electro-Nucleonics, Inc. Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer
US5981297A (en) * 1997-02-05 1999-11-09 The United States Of America As Represented By The Secretary Of The Navy Biosensor using magnetically-detected label
US6358752B1 (en) * 1996-09-27 2002-03-19 Cornell Research Foundation, Inc. Liposome-enhanced test device and method
US6576460B1 (en) * 1999-10-28 2003-06-10 Cornell Research Foundation, Inc. Filtration-detection device and method of use
WO2003102541A2 (en) * 2002-05-31 2003-12-11 Cornell Research Foundation, Inc. Universal biosensor and methods of use

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4372745A (en) * 1979-12-19 1983-02-08 Electro-Nucleonics, Inc. Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer
US6358752B1 (en) * 1996-09-27 2002-03-19 Cornell Research Foundation, Inc. Liposome-enhanced test device and method
US5981297A (en) * 1997-02-05 1999-11-09 The United States Of America As Represented By The Secretary Of The Navy Biosensor using magnetically-detected label
US6576460B1 (en) * 1999-10-28 2003-06-10 Cornell Research Foundation, Inc. Filtration-detection device and method of use
WO2003102541A2 (en) * 2002-05-31 2003-12-11 Cornell Research Foundation, Inc. Universal biosensor and methods of use

Also Published As

Publication number Publication date
WO2005084404A2 (en) 2005-09-15

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