WO2005077104A3 - Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays - Google Patents

Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays Download PDF

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Publication number
WO2005077104A3
WO2005077104A3 PCT/US2005/004481 US2005004481W WO2005077104A3 WO 2005077104 A3 WO2005077104 A3 WO 2005077104A3 US 2005004481 W US2005004481 W US 2005004481W WO 2005077104 A3 WO2005077104 A3 WO 2005077104A3
Authority
WO
WIPO (PCT)
Prior art keywords
substrate
cell
impedance
monitoring
methods
Prior art date
Application number
PCT/US2005/004481
Other languages
French (fr)
Other versions
WO2005077104A2 (en
Inventor
Xiao Xu
Yama Abassi
Xiaobo Wang
Original Assignee
Xiao Xu
Yama Abassi
Xiaobo Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/987,732 external-priority patent/US7192752B2/en
Priority to CA002556219A priority Critical patent/CA2556219A1/en
Priority to CN2005800122559A priority patent/CN101400780B/en
Priority to EP05722991A priority patent/EP1749203A4/en
Application filed by Xiao Xu, Yama Abassi, Xiaobo Wang filed Critical Xiao Xu
Priority to US11/198,831 priority patent/US8263375B2/en
Priority to US11/197,994 priority patent/US7468255B2/en
Publication of WO2005077104A2 publication Critical patent/WO2005077104A2/en
Priority to EP05812268A priority patent/EP1800312A4/en
Priority to PCT/US2005/034561 priority patent/WO2006036952A2/en
Priority to CA002580548A priority patent/CA2580548A1/en
Priority to US11/235,938 priority patent/US7732127B2/en
Priority to EP05852157.6A priority patent/EP1815025B1/en
Priority to US11/286,882 priority patent/US8206903B2/en
Priority to PCT/US2005/042684 priority patent/WO2006058185A2/en
Publication of WO2005077104A3 publication Critical patent/WO2005077104A3/en
Priority to US13/403,976 priority patent/US10215748B2/en
Priority to US13/569,156 priority patent/US8916357B2/en
Priority to US14/541,024 priority patent/US10067121B2/en
Priority to US15/612,688 priority patent/US10620188B2/en
Priority to US15/613,052 priority patent/US10168318B2/en
Priority to US16/237,382 priority patent/US10725023B2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • G01N33/4836—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures using multielectrode arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C12M25/08—Plates; Walls; Drawers; Multilayer plates electrically charged
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/021—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance before and after chemical transformation of the material

Abstract

The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.
PCT/US2005/004481 2002-07-20 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays WO2005077104A2 (en)

Priority Applications (18)

Application Number Priority Date Filing Date Title
CA002556219A CA2556219A1 (en) 2004-02-09 2005-02-09 Methods for assaying cells using cell-substrate impedance monitoring
CN2005800122559A CN101400780B (en) 2004-02-09 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
EP05722991A EP1749203A4 (en) 2004-02-09 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
US11/198,831 US8263375B2 (en) 2002-12-20 2005-08-04 Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US11/197,994 US7468255B2 (en) 2002-12-20 2005-08-04 Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology
EP05812268A EP1800312A4 (en) 2004-09-27 2005-09-27 Dynamic monitoring of cell adhesion and spreading using the rt-ces system
PCT/US2005/034561 WO2006036952A2 (en) 2004-09-27 2005-09-27 Monitoring of cell adhesion and spreading
CA002580548A CA2580548A1 (en) 2004-09-27 2005-09-27 Monitoring of cell adhesion and spreading
US11/235,938 US7732127B2 (en) 2002-12-20 2005-09-27 Dynamic monitoring of cell adhesion and spreading using the RT-CES system
EP05852157.6A EP1815025B1 (en) 2004-11-24 2005-11-23 Dynamic monitoring of cell responses to the delivery of molecules into the cell
PCT/US2005/042684 WO2006058185A2 (en) 2004-11-24 2005-11-23 Device and method for electroporation-based delivery of moecules into cells and dynamic monitoring of cell responses
US11/286,882 US8206903B2 (en) 2002-12-20 2005-11-23 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
US13/403,976 US10215748B2 (en) 2002-12-20 2012-02-23 Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
US13/569,156 US8916357B2 (en) 2002-12-20 2012-08-07 Dynamic monitoring of activation of receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US14/541,024 US10067121B2 (en) 2002-07-20 2014-11-13 Dynamic monitoring of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) activity and pathways in living cells using real-time microelectronic cell sensing technology
US15/612,688 US10620188B2 (en) 2002-12-20 2017-06-02 Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
US15/613,052 US10168318B2 (en) 2002-12-20 2017-06-02 Method of measuring cell-substrate impedance in living cells to identify compounds affecting receptor tyrosine kinase (RTK) activity and pathways
US16/237,382 US10725023B2 (en) 2002-12-20 2018-12-31 Method of measuring cell-substrate impedance in living cells to identify compounds affecting receptor tyrosine kinase (RTK) activity and pathways for the treatment of cancer

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US54292704P 2004-02-09 2004-02-09
US60/542,927 2004-02-09
US54871304P 2004-02-27 2004-02-27
US60/548,713 2004-02-27
US61460104P 2004-09-29 2004-09-29
US60/614,601 2004-09-29
US10/987,732 2004-11-12
US10/987,732 US7192752B2 (en) 2002-12-20 2004-11-12 Real time electronic cell sensing systems and applications for cell-based assays

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2004/037696 Continuation-In-Part WO2005047482A2 (en) 2002-07-20 2004-11-12 Real time electronic cell sensing systems and applications for cell-based assays
US11/055,639 Continuation-In-Part US7560269B2 (en) 2002-07-20 2005-02-09 Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US11/055,639 Continuation-In-Part US7560269B2 (en) 2002-07-20 2005-02-09 Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
US11/198,831 Continuation-In-Part US8263375B2 (en) 2002-07-20 2005-08-04 Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US11/197,994 Continuation-In-Part US7468255B2 (en) 2002-07-20 2005-08-04 Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology

Publications (2)

Publication Number Publication Date
WO2005077104A2 WO2005077104A2 (en) 2005-08-25
WO2005077104A3 true WO2005077104A3 (en) 2008-08-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/004481 WO2005077104A2 (en) 2002-07-20 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays

Country Status (4)

Country Link
EP (1) EP1749203A4 (en)
CN (1) CN101400780B (en)
CA (1) CA2556219A1 (en)
WO (1) WO2005077104A2 (en)

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US7560269B2 (en) 2002-12-20 2009-07-14 Acea Biosciences, Inc. Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
US8263375B2 (en) 2002-12-20 2012-09-11 Acea Biosciences Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US10215748B2 (en) 2002-12-20 2019-02-26 Acea Biosciences, Inc. Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
US11346797B2 (en) 2002-12-20 2022-05-31 Agilent Technologies, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties
US10539523B2 (en) 2002-12-20 2020-01-21 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology, and electrophysiological properties
US9612234B2 (en) 2008-05-05 2017-04-04 Acea Biosciences, Inc. Data analysis of impedance-based cardiomyocyte-beating signals as detected on real-time cell analysis (RTCA) cardio instruments
US10551371B2 (en) 2003-11-10 2020-02-04 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function
CA2550274A1 (en) 2003-11-12 2005-05-26 Acea Biosciences, Inc. Real time electronic cell sensing systems and applications for cell-based assays
EP1773979B1 (en) 2004-08-04 2016-06-29 Acea Biosciences, Inc. Dynamic monitoring of activation of g-protein (gpcr) in living cells using real-time microelectronic cell sensing technology
PL1977241T3 (en) 2006-01-04 2011-02-28 Novartis Ag Antibody-dependent cellular cytotoxicity assay
EP2064644A4 (en) * 2006-09-20 2010-12-29 Acea Biosciences Inc Use of impedance-based cytological profiling to classify cellular response profiles upon exposure to biologically active agents
EP2103933B1 (en) 2008-02-25 2011-07-27 Universität Leipzig Device and method for measuring impedance in organotypic tissues
CA2723223C (en) 2008-05-05 2017-06-06 Acea Biosciences, Inc. Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution
US8771202B2 (en) 2010-01-19 2014-07-08 Becton Dickinson And Company Electrode layout for blood test sensor strip
CN103675031B (en) * 2013-12-18 2015-09-02 江苏大学 A kind of high-flux cell detection method of toxicity
EP3035052A1 (en) 2014-12-18 2016-06-22 Helmholtz-Zentrum für Infektionsforschung GmbH Method and measuring device for determination of the growth rate of biofilm
CN108473926A (en) * 2015-11-20 2018-08-31 美国艾森生物科学公司 The cellular matrix impedance monitoring of cancer cell
WO2018161063A1 (en) 2017-03-03 2018-09-07 Acea Biosciences, Inc. METHODS AND SYSTEMS FOR FUNCTIONAL MATURATION OF iPSC AND ESC DERIVED CARDIOMYOCYTES
CN111492239A (en) * 2017-08-01 2020-08-04 安捷伦科技有限公司 Cell-matrix impedance monitoring of cancer cells of different lineages, origins or stages
US11733323B2 (en) 2018-06-13 2023-08-22 Centrul International De Biodinamica Systems and methods for measuring cellular response to target analytes by controlled application of an oscillating stimulus
RO133794A2 (en) * 2018-06-13 2019-12-30 Centrul Internaţional De Biodinamică Method and device for detecting bioactive, e.g. cytotoxic, compounds, using sensors with stimulated cells
CN111751522B (en) * 2019-03-27 2023-06-20 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 Cell detection analyzer and detection method of cell proliferation information
CN110568037B (en) * 2019-09-02 2021-12-03 江南大学 Construction method and application of electrochemical cell sensor for acetamiprid and imidacloprid combined toxicity evaluation
CN111735752A (en) * 2020-07-01 2020-10-02 京东方科技集团股份有限公司 Cell detection device and cell detection system
CN112485301B (en) * 2020-11-25 2023-07-14 三诺生物传感股份有限公司 Test method, system, equipment and medium of electrochemical test device

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Also Published As

Publication number Publication date
CN101400780B (en) 2012-06-20
EP1749203A4 (en) 2009-05-06
WO2005077104A2 (en) 2005-08-25
CN101400780A (en) 2009-04-01
CA2556219A1 (en) 2005-08-25
EP1749203A2 (en) 2007-02-07

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