WO2005071401A3 - Homogeneous multiplex assay for nucleic acid targets - Google Patents

Homogeneous multiplex assay for nucleic acid targets Download PDF

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Publication number
WO2005071401A3
WO2005071401A3 PCT/US2005/001378 US2005001378W WO2005071401A3 WO 2005071401 A3 WO2005071401 A3 WO 2005071401A3 US 2005001378 W US2005001378 W US 2005001378W WO 2005071401 A3 WO2005071401 A3 WO 2005071401A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid targets
multiplex assay
combination
homogeneous
Prior art date
Application number
PCT/US2005/001378
Other languages
French (fr)
Other versions
WO2005071401A2 (en
Inventor
Venkatakrishna Shyamala
Steve H Nguyen
Original Assignee
Chiron Corp
Venkatakrishna Shyamala
Steve H Nguyen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp, Venkatakrishna Shyamala, Steve H Nguyen filed Critical Chiron Corp
Publication of WO2005071401A2 publication Critical patent/WO2005071401A2/en
Publication of WO2005071401A3 publication Critical patent/WO2005071401A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles

Abstract

The present invention provides a method for the simultaneous analysis, in a homogeneous format, for detection of multiple nucleic acid target sequences. The invention utilizes capture probes conjugated to substrates that are distinguishable based on unique spectral signature in combination with reporter probes having a separately detectable label. In the presence of a target sequence, detectable complexes are formed that are distinguishable for each combination of target, capture probe and reporter probe.
PCT/US2005/001378 2004-01-15 2005-01-14 Homogeneous multiplex assay for nucleic acid targets WO2005071401A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53697804P 2004-01-15 2004-01-15
US60/536,978 2004-01-15

Publications (2)

Publication Number Publication Date
WO2005071401A2 WO2005071401A2 (en) 2005-08-04
WO2005071401A3 true WO2005071401A3 (en) 2005-12-01

Family

ID=34807070

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/001378 WO2005071401A2 (en) 2004-01-15 2005-01-14 Homogeneous multiplex assay for nucleic acid targets

Country Status (1)

Country Link
WO (1) WO2005071401A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2392445T3 (en) 2004-07-13 2012-12-10 Gen-Probe Incorporated Compositions and methods for the detection of hepatitis A virus nucleic acid
CA2640385C (en) * 2005-12-23 2014-07-15 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
US7534588B2 (en) * 2007-05-25 2009-05-19 Asiagen Corporation Methods, kits and polynucleotides for simultaneously diagnosing viruses
WO2011047087A2 (en) 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
JP6083849B2 (en) * 2012-07-20 2017-02-22 国立大学法人静岡大学 Method and kit for detecting or quantifying a target substance in a sample
US10011811B2 (en) 2013-11-25 2018-07-03 Vericel Corporation Cell seeding device and method
CN103630531A (en) * 2013-12-06 2014-03-12 苏州长光华医生物医学工程有限公司 Hepatitis B surface antigen measuring kit and detection method
CN103616513A (en) * 2013-12-06 2014-03-05 苏州长光华医生物医学工程有限公司 Hepatitis B virus e-antigen measurement kit and detection method thereof
CN104090101A (en) * 2014-07-21 2014-10-08 威海威高生物科技有限公司 Human immunodeficiency virus (HIV) antibody detection kit and preparation method thereof
CN111909932B (en) * 2020-07-17 2023-06-20 南方医科大学 Nanometer Jin Yingguang probe for in-situ detection of exosome multiple microRNAs and preparation method and application thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002785A2 (en) * 1986-10-14 1988-04-21 Beckman Instruments, Inc. Improved nucleic acid hybridization technique and kit therefor
US5516635A (en) * 1991-10-15 1996-05-14 Ekins; Roger P. Binding assay employing labelled reagent
US6268147B1 (en) * 1998-11-02 2001-07-31 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
WO2002040698A2 (en) * 2000-11-17 2002-05-23 Surromed, Inc. Analysis of soluble factor biological markers
WO2003002753A2 (en) * 2001-06-28 2003-01-09 Chiron Corporation Diagnostic assays for parvovirus b19
WO2003031934A2 (en) * 2001-10-09 2003-04-17 Chiron Corporation Identification of oligonucleotides for the capture, detection and quantitation of hepatitis b viral dna

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002785A2 (en) * 1986-10-14 1988-04-21 Beckman Instruments, Inc. Improved nucleic acid hybridization technique and kit therefor
US5516635A (en) * 1991-10-15 1996-05-14 Ekins; Roger P. Binding assay employing labelled reagent
US6268147B1 (en) * 1998-11-02 2001-07-31 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
WO2002040698A2 (en) * 2000-11-17 2002-05-23 Surromed, Inc. Analysis of soluble factor biological markers
WO2003002753A2 (en) * 2001-06-28 2003-01-09 Chiron Corporation Diagnostic assays for parvovirus b19
WO2003031934A2 (en) * 2001-10-09 2003-04-17 Chiron Corporation Identification of oligonucleotides for the capture, detection and quantitation of hepatitis b viral dna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SPIRO A ET AL: "A BEAD-BASED METHOD FOR MULTIPLEXED IDENTIFICATION AND QUANTITATION OF DNA SEQUENCES USING FLOW CYTOMETRY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 66, no. 10, October 2000 (2000-10-01), pages 4258 - 4265, XP001069133, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
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