WO2005060641A3 - Engineered templates and their use in single primer amplification - Google Patents

Engineered templates and their use in single primer amplification Download PDF

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Publication number
WO2005060641A3
WO2005060641A3 PCT/US2004/041945 US2004041945W WO2005060641A3 WO 2005060641 A3 WO2005060641 A3 WO 2005060641A3 US 2004041945 W US2004041945 W US 2004041945W WO 2005060641 A3 WO2005060641 A3 WO 2005060641A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide
template
primer
anneals
predetermined sequence
Prior art date
Application number
PCT/US2004/041945
Other languages
French (fr)
Other versions
WO2005060641A2 (en
Inventor
Toshiaki Maruyama
Shana Frederickson
Katherine S Bowdish
Mark Renshaw
Ying-Chi Lin
Original Assignee
Alexion Pharma Inc
Toshiaki Maruyama
Shana Frederickson
Katherine S Bowdish
Mark Renshaw
Ying-Chi Lin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharma Inc, Toshiaki Maruyama, Shana Frederickson, Katherine S Bowdish, Mark Renshaw, Ying-Chi Lin filed Critical Alexion Pharma Inc
Priority to CA2549982A priority Critical patent/CA2549982C/en
Priority to AU2004305055A priority patent/AU2004305055B2/en
Priority to EP04814163A priority patent/EP1711615A4/en
Priority to JP2006545814A priority patent/JP2007513642A/en
Publication of WO2005060641A2 publication Critical patent/WO2005060641A2/en
Publication of WO2005060641A3 publication Critical patent/WO2005060641A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4283—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig
    • C07K16/4291—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig against IgE
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
    • C07K16/082—Hepadnaviridae, e.g. hepatitis B virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/861—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving IgG3, IgG4, IgA, or IgY

Abstract

Methods of amplifying nucleic acid have now been discovered which include the steps of: a) annealing a primer to a template nucleic acid sequence, the primer having a first portion which anneals to the template and a second portion of predetermined sequence; b) synthesizing a polynucleotide that anneals to and is complementary to the portion of the template between the location at which the first portion of the primer anneals to the template and the end of the template, the polynucleotide having a first end and a second end, wherein the first end incorporates the primer; c) separating the polynucleotide synthesized in step (b) from the template; d) annealing a nested oligonucleotide to the second end of the polynucleotide synthesized in step (b), the nested oligonucleotide having a first portion that anneals to the second end of the polynucleotide and a second portion having the same predetermined sequence as the second portion of the primer; e) extending the polynucleotide synthesized in step (b) to provide a terminal portion thereof that is complementary to the predetermined sequence; and f) amplifying the extended polynucleotide using a single primer having the predetermined sequence. In particularly useful embodiments, the methods are used to amplify a repertoire of IgA antibodies.
PCT/US2004/041945 2002-09-19 2004-12-15 Engineered templates and their use in single primer amplification WO2005060641A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2549982A CA2549982C (en) 2003-12-15 2004-12-15 Engineered templates and their use in single primer amplification
AU2004305055A AU2004305055B2 (en) 2002-09-19 2004-12-15 Engineered templates and their use in single primer amplification
EP04814163A EP1711615A4 (en) 2003-12-15 2004-12-15 Engineered templates and their use in single primer amplification
JP2006545814A JP2007513642A (en) 2003-12-15 2004-12-15 Artificial templates and their use in single primer amplification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/737,252 2003-12-15
US10/737,252 US7414111B2 (en) 2001-09-19 2003-12-15 Engineered templates and their use in single primer amplification

Publications (2)

Publication Number Publication Date
WO2005060641A2 WO2005060641A2 (en) 2005-07-07
WO2005060641A3 true WO2005060641A3 (en) 2008-02-21

Family

ID=34710476

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/041945 WO2005060641A2 (en) 2002-09-19 2004-12-15 Engineered templates and their use in single primer amplification

Country Status (6)

Country Link
US (1) US7414111B2 (en)
EP (1) EP1711615A4 (en)
JP (2) JP2007513642A (en)
AU (1) AU2004305055B2 (en)
CA (1) CA2549982C (en)
WO (1) WO2005060641A2 (en)

Families Citing this family (16)

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WO2005027846A2 (en) * 2003-09-18 2005-03-31 Alexion Pharmaceuticals, Inc. Elicitation of antibodies to self peptides in mice by immunization with dendritic cells
US20050208627A1 (en) * 2003-09-18 2005-09-22 Bowdish Katherine S Elicitation of antibodies to self peptides in mice by immunization with dendritic cells
JP2010507366A (en) * 2006-10-19 2010-03-11 メルク アンド カンパニー インコーポレイテッド Antibody antagonist of interleukin-13 receptor α1
IL199534A (en) 2007-01-05 2013-01-31 Univ Zuerich Isolated human antibody which is capable of selectively recognizing a neoepitope of a disorder-associated protein, a polynucleotide encoding the antibody, a vector comprising the polynucleotide, a host cell comprising the polynucleotide or the vector, a composition comprising the antibody and methods and uses associated therewith
CA2746778C (en) 2008-12-19 2019-04-23 University Of Zurich Human anti-alpha-synuclein autoantibodies
BR112013000341A2 (en) * 2010-07-07 2017-09-26 Tubitak recombinant antibody structures by binding and blocking growth of factor 2 endothelial vascular activity (vegfr-2 / kdr)
DK3741883T3 (en) * 2010-07-16 2023-02-20 Adimab Llc ANTIBODIES LIBRARIES
AU2012272790B2 (en) * 2011-06-23 2016-10-06 Biogen International Neuroscience Gmbh Anti-alpha synuclein binding molecules
WO2014085687A1 (en) * 2012-11-28 2014-06-05 Abwiz Bio, Inc. Preparation of gene-specific templates for the use in single primer amplification
CA2906076A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
EP3950944A1 (en) 2014-09-15 2022-02-09 AbVitro LLC High-throughput nucleotide library sequencing
MA41115A (en) 2014-12-02 2017-10-10 Biogen Int Neuroscience Gmbh ALZHEIMER'S DISEASE TREATMENT PROCESS
CN117512078A (en) 2016-09-22 2024-02-06 西格马-奥尔德里奇有限责任公司 Single primer to double primer amplicon conversion
EP3672631B9 (en) 2017-08-22 2023-06-28 Biogen MA Inc. Pharmaceutical compositions containing anti-beta amyloid antibodies
US20210380721A1 (en) * 2020-06-01 2021-12-09 University Of Kentucky Research Foundation Compounds and methods for use in connection with opioid use disorders
EP4192858A1 (en) * 2020-08-10 2023-06-14 Integral Molecular, Inc. Methods and compositions for making antibody libraries and antibodies isolated from the same

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US5679512A (en) * 1992-07-31 1997-10-21 Behringwerke Ag Method for introducing defined sequences at the 3'end of polynucleotides

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Also Published As

Publication number Publication date
CA2549982C (en) 2014-07-08
AU2004305055A1 (en) 2005-07-07
JP2007513642A (en) 2007-05-31
AU2004305055B2 (en) 2010-04-22
US7414111B2 (en) 2008-08-19
CA2549982A1 (en) 2005-07-07
EP1711615A2 (en) 2006-10-18
EP1711615A4 (en) 2009-05-27
JP2012157371A (en) 2012-08-23
US20040175736A1 (en) 2004-09-09
WO2005060641A2 (en) 2005-07-07

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