WO2005047482A3 - Real time electronic cell sensing systems and applications for cell-based assays - Google Patents

Real time electronic cell sensing systems and applications for cell-based assays Download PDF

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Publication number
WO2005047482A3
WO2005047482A3 PCT/US2004/037696 US2004037696W WO2005047482A3 WO 2005047482 A3 WO2005047482 A3 WO 2005047482A3 US 2004037696 W US2004037696 W US 2004037696W WO 2005047482 A3 WO2005047482 A3 WO 2005047482A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
substrate
impedance
monitoring
arrays
Prior art date
Application number
PCT/US2004/037696
Other languages
French (fr)
Other versions
WO2005047482A2 (en
Inventor
Xiao Xu
Yama Abbassi
Xiaobo Wang
Jiangbo Gan
Original Assignee
Xiao Xu
Yama Abbassi
Xiaobo Wang
Jiangbo Gan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xiao Xu, Yama Abbassi, Xiaobo Wang, Jiangbo Gan filed Critical Xiao Xu
Priority to EP04801001A priority Critical patent/EP1692258A4/en
Priority to CA002550274A priority patent/CA2550274A1/en
Publication of WO2005047482A2 publication Critical patent/WO2005047482A2/en
Publication of WO2005047482A3 publication Critical patent/WO2005047482A3/en
Priority to US11/198,831 priority patent/US8263375B2/en
Priority to US11/197,994 priority patent/US7468255B2/en
Priority to EP05812268A priority patent/EP1800312A4/en
Priority to CA002580548A priority patent/CA2580548A1/en
Priority to US11/235,938 priority patent/US7732127B2/en
Priority to PCT/US2005/034561 priority patent/WO2006036952A2/en
Priority to US11/286,882 priority patent/US8206903B2/en
Priority to US13/403,976 priority patent/US10215748B2/en
Priority to US13/569,156 priority patent/US8916357B2/en
Priority to US14/541,024 priority patent/US10067121B2/en
Priority to US15/613,052 priority patent/US10168318B2/en
Priority to US15/612,688 priority patent/US10620188B2/en
Priority to US16/237,382 priority patent/US10725023B2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C12M25/08—Plates; Walls; Drawers; Multilayer plates electrically charged
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • G01N33/4836—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures using multielectrode arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Abstract

The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays (102) on a nonconducting substrate (101), in which each of the arrays (102) has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices (416) comprising multiple wells each having an electrode array (102), an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state.
PCT/US2004/037696 2002-07-20 2004-11-12 Real time electronic cell sensing systems and applications for cell-based assays WO2005047482A2 (en)

Priority Applications (15)

Application Number Priority Date Filing Date Title
EP04801001A EP1692258A4 (en) 2003-11-12 2004-11-12 Real time electronic cell sensing systems and applications for cell-based assays
CA002550274A CA2550274A1 (en) 2003-11-12 2004-11-12 Real time electronic cell sensing systems and applications for cell-based assays
US11/198,831 US8263375B2 (en) 2002-12-20 2005-08-04 Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US11/197,994 US7468255B2 (en) 2002-12-20 2005-08-04 Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology
PCT/US2005/034561 WO2006036952A2 (en) 2004-09-27 2005-09-27 Monitoring of cell adhesion and spreading
US11/235,938 US7732127B2 (en) 2002-12-20 2005-09-27 Dynamic monitoring of cell adhesion and spreading using the RT-CES system
CA002580548A CA2580548A1 (en) 2004-09-27 2005-09-27 Monitoring of cell adhesion and spreading
EP05812268A EP1800312A4 (en) 2004-09-27 2005-09-27 Dynamic monitoring of cell adhesion and spreading using the rt-ces system
US11/286,882 US8206903B2 (en) 2002-12-20 2005-11-23 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
US13/403,976 US10215748B2 (en) 2002-12-20 2012-02-23 Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
US13/569,156 US8916357B2 (en) 2002-12-20 2012-08-07 Dynamic monitoring of activation of receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US14/541,024 US10067121B2 (en) 2002-07-20 2014-11-13 Dynamic monitoring of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) activity and pathways in living cells using real-time microelectronic cell sensing technology
US15/613,052 US10168318B2 (en) 2002-12-20 2017-06-02 Method of measuring cell-substrate impedance in living cells to identify compounds affecting receptor tyrosine kinase (RTK) activity and pathways
US15/612,688 US10620188B2 (en) 2002-12-20 2017-06-02 Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
US16/237,382 US10725023B2 (en) 2002-12-20 2018-12-31 Method of measuring cell-substrate impedance in living cells to identify compounds affecting receptor tyrosine kinase (RTK) activity and pathways for the treatment of cancer

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US51956703P 2003-11-12 2003-11-12
US60/519,567 2003-11-12
US54292704P 2004-02-09 2004-02-09
US60/542,927 2004-02-09
US54871304P 2004-02-27 2004-02-27
US60/548,713 2004-02-27

Related Child Applications (4)

Application Number Title Priority Date Filing Date
US11/055,639 Continuation-In-Part US7560269B2 (en) 2002-07-20 2005-02-09 Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
PCT/US2005/004481 Continuation-In-Part WO2005077104A2 (en) 2002-07-20 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
US11/197,994 Continuation-In-Part US7468255B2 (en) 2002-07-20 2005-08-04 Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology
US13/403,976 Continuation-In-Part US10215748B2 (en) 2002-12-20 2012-02-23 Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways

Publications (2)

Publication Number Publication Date
WO2005047482A2 WO2005047482A2 (en) 2005-05-26
WO2005047482A3 true WO2005047482A3 (en) 2005-07-07

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/037696 WO2005047482A2 (en) 2002-07-20 2004-11-12 Real time electronic cell sensing systems and applications for cell-based assays

Country Status (4)

Country Link
US (5) US7192752B2 (en)
EP (1) EP1692258A4 (en)
CA (1) CA2550274A1 (en)
WO (1) WO2005047482A2 (en)

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