WO2005044983A3 - In vitro, multiple electrode pair array and multiple treatment cell apparatus for use in electroporation - Google Patents

In vitro, multiple electrode pair array and multiple treatment cell apparatus for use in electroporation Download PDF

Info

Publication number
WO2005044983A3
WO2005044983A3 PCT/US2004/032318 US2004032318W WO2005044983A3 WO 2005044983 A3 WO2005044983 A3 WO 2005044983A3 US 2004032318 W US2004032318 W US 2004032318W WO 2005044983 A3 WO2005044983 A3 WO 2005044983A3
Authority
WO
WIPO (PCT)
Prior art keywords
array
electrodes
electrode pair
electroporation
vitro
Prior art date
Application number
PCT/US2004/032318
Other languages
French (fr)
Other versions
WO2005044983A2 (en
Inventor
Richard E Walters
Paul K Gustavson
Original Assignee
Cyto Pulse Sciences Inc
Richard E Walters
Paul K Gustavson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cyto Pulse Sciences Inc, Richard E Walters, Paul K Gustavson filed Critical Cyto Pulse Sciences Inc
Publication of WO2005044983A2 publication Critical patent/WO2005044983A2/en
Publication of WO2005044983A3 publication Critical patent/WO2005044983A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic

Abstract

An object of the invention is to provide a multiple electrode pair array apparatus (10) for use with a multiple well plate (11) which has multiple wells distributed in a two­dimensional matrix array which has R rows and C columns. The multiple electrode pair array apparatus (10) include, a non-conductive base member (12). An array of pairs of electrodes (93) are attached to the base member (12) and project therefrom. The pairs of electrodes (93) are distributed on the base member (12) in a two-dimensional matrix array which hap R rows (16) and C columns (18) to enable registration with the two­dimensional matrix array of wells. Each pair of electrodes includes a respective first electrode (20) and a respective second electrode (22). An array of R row conductors (24) are electrically connected to corresponding first electrodes (20). An array of C column conductors (26) are oriented perpendicular to the R row conductors (24), are electrically insulated from the row conductors (24), and are electrically connected to corresponding second electrodes (22).
PCT/US2004/032318 2003-10-28 2004-10-25 In vitro, multiple electrode pair array and multiple treatment cell apparatus for use in electroporation WO2005044983A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/694,345 US7371561B2 (en) 2003-10-28 2003-10-28 In vitro, multiple electrode pair array and multiple treatment cell apparatus for use in electroporation
US10/694,345 2003-10-28

Publications (2)

Publication Number Publication Date
WO2005044983A2 WO2005044983A2 (en) 2005-05-19
WO2005044983A3 true WO2005044983A3 (en) 2006-01-26

Family

ID=34522588

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/032318 WO2005044983A2 (en) 2003-10-28 2004-10-25 In vitro, multiple electrode pair array and multiple treatment cell apparatus for use in electroporation

Country Status (2)

Country Link
US (1) US7371561B2 (en)
WO (1) WO2005044983A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2294757T3 (en) 2004-03-15 2008-04-01 Amaxa Ag CONTAINER AND DEVICE FOR GENERATING ELECTRIC FIELDS IN INDIVIDUAL REACTION SPACES.
US20070004025A1 (en) * 2005-06-30 2007-01-04 Junne-Jih Chen Replaceable annular electrode for transgenic
WO2009029519A2 (en) * 2007-08-24 2009-03-05 David Mark Evans Multiplexed electroporation apparatus
NO2208780T3 (en) * 2009-01-20 2018-03-31
EP2208779B1 (en) 2009-01-20 2017-04-05 Lonza Cologne GmbH Container with multiple reaction chambers and electrodes
EP2208778A1 (en) 2009-01-20 2010-07-21 Lonza Cologne AG Method and device for electric processing of reaction areas
CN103275874B (en) * 2013-06-08 2014-09-10 苏州文曲生物微系统有限公司 High-density distributed stereo electrode apparatus
US20210247357A1 (en) * 2018-03-26 2021-08-12 National Institute For Materials Science Electrochemical measurement system, electrochemical search method, reactor, and microplate

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4695547A (en) * 1986-04-02 1987-09-22 Jeffrey L. Hilliard Probe for electrofusion, electroporation, or like procedure
US5187096A (en) * 1991-08-08 1993-02-16 Rensselaer Polytechnic Institute Cell substrate electrical impedance sensor with multiple electrode array
US5643742A (en) * 1990-04-03 1997-07-01 Cellstat Technologies, Inc. System for electronically monitoring and recording cell cultures
US6117660A (en) * 1997-06-10 2000-09-12 Cytopulse Sciences, Inc. Method and apparatus for treating materials with electrical fields having varying orientations
US6352853B1 (en) * 1998-12-07 2002-03-05 Rosetta Inpharmatics, Inc. Multi-channel electrode arrays

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399599B2 (en) * 2000-07-10 2008-07-15 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4695547A (en) * 1986-04-02 1987-09-22 Jeffrey L. Hilliard Probe for electrofusion, electroporation, or like procedure
US5643742A (en) * 1990-04-03 1997-07-01 Cellstat Technologies, Inc. System for electronically monitoring and recording cell cultures
US5187096A (en) * 1991-08-08 1993-02-16 Rensselaer Polytechnic Institute Cell substrate electrical impedance sensor with multiple electrode array
US6117660A (en) * 1997-06-10 2000-09-12 Cytopulse Sciences, Inc. Method and apparatus for treating materials with electrical fields having varying orientations
US6352853B1 (en) * 1998-12-07 2002-03-05 Rosetta Inpharmatics, Inc. Multi-channel electrode arrays

Also Published As

Publication number Publication date
WO2005044983A2 (en) 2005-05-19
US7371561B2 (en) 2008-05-13
US20050089992A1 (en) 2005-04-28

Similar Documents

Publication Publication Date Title
DE502006003889D1 (en) DEVICE FOR THE ELECTRICAL STIMULATION OF BIOLOGICAL MATERIAL
WO2006113593A3 (en) Electrical stimulation lead, system, and method
WO2002078592A3 (en) Systems and methods for selectively stimulating components in, on, or near the pudendal nerve or its branches to achieve selective physiologic responses
TWI263183B (en) Display apparatus
WO2005011805A3 (en) Apparatus and methods for applying neural stimulation to a patient
AU2003223351A1 (en) Method of treating biological materials with translating electrical fields and electrode polarity reversal
WO2004095535A3 (en) Improved transparent electrode, optoelectronic apparatus and devices
TW200712998A (en) Two-dimensional position sensor
WO2017062577A3 (en) Implantable modular electrode array assembly
WO2003082402A3 (en) Electrode geometries for efficient neural stimulation
EP1785160A3 (en) Apparatus for nerve stimulation
EP0883191A3 (en) Electroluminescence device, electroluminescence apparatus, and production methods thereof
WO2004061417A3 (en) Device structure for closely spaced electrodes
EP1536450A3 (en) Plasma display panel
WO2003019346A1 (en) Touch sensor, display with touch sensor, and method for generating position data
WO2003030304A3 (en) High speed docking connector
EP1505696A3 (en) Multi-element connector
WO2004010103A3 (en) Impedance based devices and methods for use in assays
WO2007117728A3 (en) Non-linear electrode array
EP1473690A3 (en) Drive circuitry for four-color organic light-emitting device
WO2003026736A3 (en) Methods and implantable apparatus for electrical therapy
WO2007005641A3 (en) Deployable epicardial electrode and sensor array
WO2006127320A3 (en) Electroplating apparatus based on an array of anodes
TW200625709A (en) Vertical interconnect for organic electronic devices
WO2008156852A3 (en) Connector with uniformly arranged ground and signal tail contact portions

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 69(1) EPC (EPO FORM 1205A DATED 22.08.2006 )

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 69(1) EPC (EPO FORM 1205A DATED 22-08-2006 )

122 Ep: pct application non-entry in european phase