WO2005030984A3 - Method for small volume nucleic acid synthesis - Google Patents

Method for small volume nucleic acid synthesis Download PDF

Info

Publication number
WO2005030984A3
WO2005030984A3 PCT/US2004/031804 US2004031804W WO2005030984A3 WO 2005030984 A3 WO2005030984 A3 WO 2005030984A3 US 2004031804 W US2004031804 W US 2004031804W WO 2005030984 A3 WO2005030984 A3 WO 2005030984A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
small volume
acid synthesis
cdna
volume nucleic
Prior art date
Application number
PCT/US2004/031804
Other languages
French (fr)
Other versions
WO2005030984A2 (en
Inventor
James M Kadushin
Original Assignee
Datascope Investment Corp
James M Kadushin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Datascope Investment Corp, James M Kadushin filed Critical Datascope Investment Corp
Priority to JP2006528313A priority Critical patent/JP2007506439A/en
Priority to EP04789156A priority patent/EP1664350A4/en
Publication of WO2005030984A2 publication Critical patent/WO2005030984A2/en
Publication of WO2005030984A3 publication Critical patent/WO2005030984A3/en
Priority to IL174486A priority patent/IL174486A0/en
Priority to US11/584,751 priority patent/US20080033159A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

A method for synthesis of nucleic acids, with particular use for synthesis of cDNA. The method allows application of the cDNA to a microarray without the need for concentration or purification of the cDNA post-cDNA synthesis.
PCT/US2004/031804 2001-01-13 2004-09-27 Method for small volume nucleic acid synthesis WO2005030984A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2006528313A JP2007506439A (en) 2003-09-26 2004-09-27 Method for synthesizing a small amount of nucleic acid
EP04789156A EP1664350A4 (en) 2003-09-26 2004-09-27 Method for small volume nucleic acid synthesis
IL174486A IL174486A0 (en) 2003-09-26 2006-03-22 Method for small volume nucleic acid synthesis
US11/584,751 US20080033159A1 (en) 2001-01-13 2006-10-20 Method for small volume nucleic acid synthesis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50624703P 2003-09-26 2003-09-26
US60/506,247 2003-09-26

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
US10/234,069 Continuation-In-Part US20040009487A1 (en) 2001-01-13 2002-09-03 Methods for blocking nonspecific hybridizations of nucleic acid sequences
PCT/US2003/009232 Continuation-In-Part WO2004061126A2 (en) 2000-03-08 2003-03-25 Nucleic acid hybridization assay using bridging sequence

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US38877206A Continuation 2001-01-13 2006-03-24

Publications (2)

Publication Number Publication Date
WO2005030984A2 WO2005030984A2 (en) 2005-04-07
WO2005030984A3 true WO2005030984A3 (en) 2005-12-22

Family

ID=34393128

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/031804 WO2005030984A2 (en) 2001-01-13 2004-09-27 Method for small volume nucleic acid synthesis

Country Status (4)

Country Link
EP (1) EP1664350A4 (en)
JP (1) JP2007506439A (en)
IL (1) IL174486A0 (en)
WO (1) WO2005030984A2 (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing

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US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
EP2001990B1 (en) 2006-03-24 2016-06-29 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
CA2833262C (en) 2011-04-15 2020-08-18 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
CN104040238B (en) 2011-11-04 2017-06-27 汉迪拉布公司 Polynucleotides sample preparation apparatus
CA2863637C (en) 2012-02-03 2021-10-26 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5487973A (en) * 1986-09-10 1996-01-30 Polyprobe, Inc. Methods for detecting and assaying nucleic acid sequences
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US6582906B1 (en) * 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2400680A1 (en) * 2000-03-08 2001-09-13 Datascope Investment Corp. Methods for assay and detection on a microarray

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5487973A (en) * 1986-09-10 1996-01-30 Polyprobe, Inc. Methods for detecting and assaying nucleic acid sequences
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US6582906B1 (en) * 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US11845081B2 (en) 2007-07-13 2023-12-19 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
US9480983B2 (en) 2011-09-30 2016-11-01 Becton, Dickinson And Company Unitized reagent strip

Also Published As

Publication number Publication date
EP1664350A4 (en) 2007-10-10
IL174486A0 (en) 2006-08-01
JP2007506439A (en) 2007-03-22
WO2005030984A2 (en) 2005-04-07
EP1664350A2 (en) 2006-06-07

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