WO2005012329A3 - Kinase and phosphatase assays - Google Patents

Kinase and phosphatase assays Download PDF

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Publication number
WO2005012329A3
WO2005012329A3 PCT/US2004/024359 US2004024359W WO2005012329A3 WO 2005012329 A3 WO2005012329 A3 WO 2005012329A3 US 2004024359 W US2004024359 W US 2004024359W WO 2005012329 A3 WO2005012329 A3 WO 2005012329A3
Authority
WO
WIPO (PCT)
Prior art keywords
kinase
peptide
phosphatase
phosphatase assays
phosphatase activity
Prior art date
Application number
PCT/US2004/024359
Other languages
French (fr)
Other versions
WO2005012329A2 (en
Inventor
Elizabeth A Werner
Tony A Klink
Jane A Beebe
David A Lasky
Karen M Kleman-Leyer
Richard Somberg
Original Assignee
Invitrogen Corp
Elizabeth A Werner
Tony A Klink
Jane A Beebe
David A Lasky
Karen M Kleman-Leyer
Richard Somberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34102992&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2005012329(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Invitrogen Corp, Elizabeth A Werner, Tony A Klink, Jane A Beebe, David A Lasky, Karen M Kleman-Leyer, Richard Somberg filed Critical Invitrogen Corp
Priority to EP04779423A priority Critical patent/EP1664332B1/en
Priority to JP2006522034A priority patent/JP2007500710A/en
Priority to AT04779423T priority patent/ATE466098T1/en
Priority to DE602004026888T priority patent/DE602004026888D1/en
Publication of WO2005012329A2 publication Critical patent/WO2005012329A2/en
Publication of WO2005012329A3 publication Critical patent/WO2005012329A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/81—Protease inhibitors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases

Abstract

Compositions, methods, and kits for detecting and monitoring kinase or phosphatase activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase or phosphatase alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase or phosphatase activity are also described.
PCT/US2004/024359 2003-07-29 2004-07-29 Kinase and phosphatase assays WO2005012329A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP04779423A EP1664332B1 (en) 2003-07-29 2004-07-29 Kinase and phosphatase assays
JP2006522034A JP2007500710A (en) 2003-07-29 2004-07-29 Kinase and phosphatase assays
AT04779423T ATE466098T1 (en) 2003-07-29 2004-07-29 KINASE AND PHOSPHATASE TESTS
DE602004026888T DE602004026888D1 (en) 2003-07-29 2004-07-29 KINASE AND PHOSPHATE ASSAYS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49077103P 2003-07-29 2003-07-29
US60/490,771 2003-07-29

Publications (2)

Publication Number Publication Date
WO2005012329A2 WO2005012329A2 (en) 2005-02-10
WO2005012329A3 true WO2005012329A3 (en) 2005-09-29

Family

ID=34102992

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/024359 WO2005012329A2 (en) 2003-07-29 2004-07-29 Kinase and phosphatase assays

Country Status (7)

Country Link
US (1) US7582461B2 (en)
EP (2) EP1664332B1 (en)
JP (1) JP2007500710A (en)
AT (1) ATE466098T1 (en)
CA (1) CA2445420A1 (en)
DE (1) DE602004026888D1 (en)
WO (1) WO2005012329A2 (en)

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DE602004026888D1 (en) 2010-06-10
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US7582461B2 (en) 2009-09-01
ATE466098T1 (en) 2010-05-15
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CA2445420A1 (en) 2005-01-29

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