WO2005009202A3 - Automatic identification of bioagents - Google Patents

Automatic identification of bioagents Download PDF

Info

Publication number
WO2005009202A3
WO2005009202A3 PCT/US2004/015123 US2004015123W WO2005009202A3 WO 2005009202 A3 WO2005009202 A3 WO 2005009202A3 US 2004015123 W US2004015123 W US 2004015123W WO 2005009202 A3 WO2005009202 A3 WO 2005009202A3
Authority
WO
WIPO (PCT)
Prior art keywords
deck
sample
bioagents
airlock
atmospheric pressure
Prior art date
Application number
PCT/US2004/015123
Other languages
French (fr)
Other versions
WO2005009202A2 (en
Inventor
John Mcneil
David Ecker
Steven A Hofstadler
Lawrence Blyn
Isaac Gesser
Lendell L Cummins
Alan Goates
Jared J Drader
Original Assignee
Isis Pharmaceuticals Inc
John Mcneil
David Ecker
Steven A Hofstadler
Lawrence Blyn
Isaac Gesser
Lendell L Cummins
Alan Goates
Jared J Drader
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Pharmaceuticals Inc, John Mcneil, David Ecker, Steven A Hofstadler, Lawrence Blyn, Isaac Gesser, Lendell L Cummins, Alan Goates, Jared J Drader filed Critical Isis Pharmaceuticals Inc
Publication of WO2005009202A2 publication Critical patent/WO2005009202A2/en
Publication of WO2005009202A3 publication Critical patent/WO2005009202A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/0092—Scheduling
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Abstract

Automated rapid identification of unknown bioagents is enabled using a self-contained device coupled to a system executive module. The device includes two decks separated by an airlock and a storage area for storing reagents and filter plates for use in identifying bioagents. A programmable robot assembly isolates and purifies DNA from the bioagent in the first deck and prepares the sample for PCR. The first deck is preferably maintained at a higher atmospheric pressure than the area external to the device, while the second deck is maintained at a lower atmospheric pressure than the external area to avoid contamination. After isolation and purification, the sample is passed through the airlock to the second deck, where it undergoes PCR. After washing and eluting of the sample, the molecular mass of the sample is determined by mass spectroscopy. The system executive provides automated request handling, job scheduling, resource management and drill-down analysis.
PCT/US2004/015123 2003-05-12 2004-05-12 Automatic identification of bioagents WO2005009202A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47017503P 2003-05-12 2003-05-12
US60/470,175 2003-05-12

Publications (2)

Publication Number Publication Date
WO2005009202A2 WO2005009202A2 (en) 2005-02-03
WO2005009202A3 true WO2005009202A3 (en) 2005-11-10

Family

ID=34102619

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/015123 WO2005009202A2 (en) 2003-05-12 2004-05-12 Automatic identification of bioagents

Country Status (1)

Country Link
WO (1) WO2005009202A2 (en)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040121313A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in organs for transplantation
WO2004060278A2 (en) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Methods for rapid identification of pathogens in humans and animals
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US8046171B2 (en) 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20120122103A1 (en) 2003-09-11 2012-05-17 Rangarajan Sampath Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8163895B2 (en) 2003-12-05 2012-04-24 Ibis Biosciences, Inc. Compositions for use in identification of orthopoxviruses
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
EP2458619B1 (en) 2004-05-24 2017-08-02 Ibis Biosciences, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
CA2600184A1 (en) 2005-03-03 2006-09-08 Isis Pharmaceuticals, Inc. Compositions for use in identification of adventitious viruses
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US8026084B2 (en) 2005-07-21 2011-09-27 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
US9149473B2 (en) 2006-09-14 2015-10-06 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
WO2008104002A2 (en) 2007-02-23 2008-08-28 Ibis Biosciences, Inc. Methods for rapid forensic dna analysis
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
EP2349549B1 (en) 2008-09-16 2012-07-18 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, and system
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
US8148163B2 (en) 2008-09-16 2012-04-03 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
WO2010093943A1 (en) 2009-02-12 2010-08-19 Ibis Biosciences, Inc. Ionization probe assemblies
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
EP2454000A4 (en) 2009-07-17 2016-08-10 Ibis Biosciences Inc Systems for bioagent identification
ES2628739T3 (en) 2009-10-15 2017-08-03 Ibis Biosciences, Inc. Multiple displacement amplification
JP5623888B2 (en) * 2009-12-10 2014-11-12 エフ．ホフマン−ラ ロシュアーゲーＦ．Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Method for separating and detecting analytes
CA2724132C (en) * 2009-12-10 2018-03-06 F. Hoffmann-La Roche Ag Amplification system with spatial separation
JP5846773B2 (en) 2010-06-29 2016-01-20 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Sample distribution
DE202011111050U1 (en) 2010-10-29 2018-11-20 Thermo Fisher Scientific Oy Automated system for sample preparation and analysis
AU2015261711B2 (en) * 2010-10-29 2017-11-09 Thermo Fisher Scientific Oy Automated system for sample preparation and analysis
WO2014110282A1 (en) 2013-01-09 2014-07-17 Siemens Healthcare Diagnostics Inc. Throughput optimizing reagent distribution
NL2024699B1 (en) 2020-01-17 2021-10-13 In Ovo Holding B V Egg Characteristic Determining Device
TR202009555A2 (en) * 2020-06-19 2020-07-21 Intecro Robotik Otomasyon Ar Ge Muehendislik Makine Sanayi Ve Ticaret A S Robotic mechanism unit in vitro diagnostic tests and analysis
NL2026004B1 (en) 2020-07-05 2022-03-11 In Ovo Holding B V Egg Determining Method and Device
CN114369525B (en) * 2020-10-19 2023-06-27 成都瀚辰光翼生物工程有限公司 Gene detection apparatus

Citations (3)

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US20020028923A1 (en) * 1998-04-13 2002-03-07 Lex M. Cowsert Identification of genetic targets for modulation by oligonucleotides and generation of oligonucleotides for gene modulation
US20020090320A1 (en) * 2000-10-13 2002-07-11 Irm Llc, A Delaware Limited Liability Company High throughput processing system and method of using
US6856914B1 (en) * 1999-11-19 2005-02-15 The University Of British Columbia Method, apparatus, media and signals for identifying associated cell signaling proteins

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020028923A1 (en) * 1998-04-13 2002-03-07 Lex M. Cowsert Identification of genetic targets for modulation by oligonucleotides and generation of oligonucleotides for gene modulation
US6856914B1 (en) * 1999-11-19 2005-02-15 The University Of British Columbia Method, apparatus, media and signals for identifying associated cell signaling proteins
US20020090320A1 (en) * 2000-10-13 2002-07-11 Irm Llc, A Delaware Limited Liability Company High throughput processing system and method of using

Also Published As

Publication number Publication date
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