WO2004105685A3 - Displaceable linker solid phase chemical ligation - Google Patents

Displaceable linker solid phase chemical ligation Download PDF

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Publication number
WO2004105685A3
WO2004105685A3 PCT/US2004/015435 US2004015435W WO2004105685A3 WO 2004105685 A3 WO2004105685 A3 WO 2004105685A3 US 2004015435 W US2004015435 W US 2004015435W WO 2004105685 A3 WO2004105685 A3 WO 2004105685A3
Authority
WO
WIPO (PCT)
Prior art keywords
ligation
chemical ligation
solid phase
compositions
methods
Prior art date
Application number
PCT/US2004/015435
Other languages
French (fr)
Other versions
WO2004105685A2 (en
Inventor
Donald Low
Ying Hu
Paolo Botti
Original Assignee
Gryphon Therapeutics Inc
Donald Low
Ying Hu
Paolo Botti
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gryphon Therapeutics Inc, Donald Low, Ying Hu, Paolo Botti filed Critical Gryphon Therapeutics Inc
Priority to EP04752449A priority Critical patent/EP1631597A4/en
Publication of WO2004105685A2 publication Critical patent/WO2004105685A2/en
Publication of WO2004105685A3 publication Critical patent/WO2004105685A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/042—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers characterised by the nature of the carrier
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups

Abstract

The invention is directed to methods and compositions for aqueous-compatible solid phase chemical ligation. The methods and compositions of the invention involve the chemical ligation of first and second polymers, where the first polymer is attached to a support through a displaceable linker. The displaceable linker can be cleaved or otherwise displaced under aqueous conditions compatible with subsequent removal of the ligation product from the solid support. The methods and compositions of the invention are particularly useful for ligation of peptides and polypeptides on a solid support. The ligation system of the invention is applicable to a wide variety of molecules, and thus can be exploited to generate ligated polymers including peptides, polypeptides and other amino acid containing polymers.
PCT/US2004/015435 2003-05-22 2004-05-17 Displaceable linker solid phase chemical ligation WO2004105685A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP04752449A EP1631597A4 (en) 2003-05-22 2004-05-17 Displaceable linker solid phase chemical ligation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47329403P 2003-05-22 2003-05-22
US60/473,294 2003-05-22

Publications (2)

Publication Number Publication Date
WO2004105685A2 WO2004105685A2 (en) 2004-12-09
WO2004105685A3 true WO2004105685A3 (en) 2005-06-09

Family

ID=33490583

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/015435 WO2004105685A2 (en) 2003-05-22 2004-05-17 Displaceable linker solid phase chemical ligation

Country Status (2)

Country Link
EP (1) EP1631597A4 (en)
WO (1) WO2004105685A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040248162A1 (en) * 2002-12-17 2004-12-09 Affymetrix, Inc. Releasable polymer arrays
US7547775B2 (en) 2004-12-31 2009-06-16 Affymetrix, Inc. Parallel preparation of high fidelity probes in an array format
GB0505200D0 (en) * 2005-03-14 2005-04-20 Univ Southampton Process
ATE439140T1 (en) 2005-03-31 2009-08-15 Amylin Pharmaceuticals Inc AMYLIN AND AMYLIN AGONISTS FOR THE TREATMENT OF PSYCHIATRIC DISEASES AND DISORDERS
EP2120985B1 (en) 2007-02-05 2012-06-27 Amylin Pharmaceuticals, Inc. FN-38 peptides for use in the treatment of psychotic and anxiety disorders
US8409526B2 (en) * 2010-12-09 2013-04-02 General Electric Company Cellulose substrates, compositions and methods for storage and analysis of biological materials
HUE034308T2 (en) 2013-03-21 2018-02-28 Sanofi Aventis Deutschland Synthesis of hydantoin containing peptide products
DK2976325T3 (en) 2013-03-21 2017-06-06 Sanofi Aventis Deutschland SYNTHESIS OF PEPTIDE PRODUCTS CONTAINING CYCLIC IMID
DK3044204T3 (en) 2013-09-11 2018-02-19 Univ Danmarks Tekniske PHOTOLABLE LINKS FOR PHASE PHYSICAL SYNTHESIS OF HYDRAZIDES AND PYRANOPYRAZOLES
JP2022508558A (en) 2018-10-01 2022-01-19 ユニベルシテ ド ジュネーヴ How to generate multiple polypeptide variants suitable for biological analysis

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141966A (en) * 1989-12-19 1992-08-25 Exploaterings Ab T.B.F. Metalchelating, hydrophilic polymer
US6271345B1 (en) * 1996-07-03 2001-08-07 Basf Aktiengesellschaft Enzyme cleavable linker bound to solid phase for organic compound synthesis
US6326468B1 (en) * 1997-06-13 2001-12-04 Gryphon Sciences Solid phase native chemical ligation of unprotected or n-terminal cysteine protected peptides in aqueous solution

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003300416A1 (en) * 2002-12-30 2004-07-29 Gryphon Therapeutics, Inc. Multiplex polymer ligation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141966A (en) * 1989-12-19 1992-08-25 Exploaterings Ab T.B.F. Metalchelating, hydrophilic polymer
US6271345B1 (en) * 1996-07-03 2001-08-07 Basf Aktiengesellschaft Enzyme cleavable linker bound to solid phase for organic compound synthesis
US6326468B1 (en) * 1997-06-13 2001-12-04 Gryphon Sciences Solid phase native chemical ligation of unprotected or n-terminal cysteine protected peptides in aqueous solution

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BRIK A. ET AL: "Protein Synthesis by Solid-Phase Chemical Ligation Using a Safety Catch Linker", J. ORG. CHEM., vol. 65, 2000, pages 3829 - 3835, XP002987909 *
CANNE L.E. ET AL: "Chemical Protein Synthesis by Solid Phase Ligation of Unprotected Peptide Segments", J. AM. CHEM. SOC., vol. 121, 1999, pages 8720 - 8727, XP002987910 *
ENGLEBRETSEN D.R. ET AL: "A Novel Thioether Linker: Chemical Synthesis of a HIV-1 Protease Analogue by Thioether Ligation", TETRAHEDRON LETTERS, vol. 36, no. 48, 1995, pages 8871 - 8874, XP004026856 *
GAERTNER H.F. ET AL: "Site-Specific Religation of G-CSF Fragments through a Thioether Bond", BIOCONJ. CHEM., vol. 5, 1994, pages 333 - 338, XP002987911 *
ROSE K. ET AL: "Facile Synthesis of Homogeneous Artificial Proteins", J. AM. CHEM. SOC., vol. 116, 1994, pages 30 - 33, XP002301786 *
ROUTLEDGE A.R. ET AL: "The Use of a Dithiane Protected Benzoin Photolabile Safety Catch Linker for Solid-Phase Synthesis", TETRA. LETTERS, vol. 38, no. 7, 1997, pages 1227 - 1230, XP004034241 *
SCHNOLZER M. ET AL: "Constructing Proteins by Dovetailing Unprotected Synthetic Peptides: Backbone-Engineered HIV Protease", SCIENCE, vol. 256, no. 5054, 10 April 1992 (1992-04-10), pages 221 - 225, XP002078246 *
See also references of EP1631597A4 *
SEITZ O. ET AL: "HYCRON, an Allylic Anchor for High-Efficiency Solid Phase Synthesis of Protected Peptides and Glycopeptides", J. ORG. CHEM., vol. 62, 1997, pages 813 - 826, XP002162441 *
ZHANG L.Z. ET AL: "Preparation of functionally active cell-permeable peptides by single-step ligation of two peptide modules", PNAS, vol. 95, August 1998 (1998-08-01), pages 9184 - 9189, XP002133028 *

Also Published As

Publication number Publication date
EP1631597A2 (en) 2006-03-08
WO2004105685A2 (en) 2004-12-09
EP1631597A4 (en) 2007-07-11

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