WO2004094635A3 - Compositions and methods for using a solid support to purify rna - Google Patents

Compositions and methods for using a solid support to purify rna Download PDF

Info

Publication number
WO2004094635A3
WO2004094635A3 PCT/US2004/012033 US2004012033W WO2004094635A3 WO 2004094635 A3 WO2004094635 A3 WO 2004094635A3 US 2004012033 W US2004012033 W US 2004012033W WO 2004094635 A3 WO2004094635 A3 WO 2004094635A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
solid support
purify rna
rna
Prior art date
Application number
PCT/US2004/012033
Other languages
French (fr)
Other versions
WO2004094635A2 (en
Inventor
Robert J Bair Jr
Ellen M Heath
Heather Meehan
Kim E Paulsen
John M Wages Jr
Original Assignee
Gentra Systems Inc
Robert J Bair Jr
Ellen M Heath
Heather Meehan
Kim E Paulsen
John M Wages Jr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gentra Systems Inc, Robert J Bair Jr, Ellen M Heath, Heather Meehan, Kim E Paulsen, John M Wages Jr filed Critical Gentra Systems Inc
Priority to CA2522446A priority Critical patent/CA2522446C/en
Priority to JP2006513124A priority patent/JP4804347B2/en
Priority to EP04760008.5A priority patent/EP1618194B1/en
Priority to AU2004233035A priority patent/AU2004233035B2/en
Publication of WO2004094635A2 publication Critical patent/WO2004094635A2/en
Publication of WO2004094635A3 publication Critical patent/WO2004094635A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Reagents, methods and kits for the purification of RNA from biological materials are provided.
PCT/US2004/012033 2001-10-12 2004-04-15 Compositions and methods for using a solid support to purify rna WO2004094635A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2522446A CA2522446C (en) 2003-04-16 2004-04-15 Compositions and methods for using a solid support to purify rna
JP2006513124A JP4804347B2 (en) 2003-04-16 2004-04-15 Compositions and methods for purifying RNA using a solid support
EP04760008.5A EP1618194B1 (en) 2003-04-16 2004-04-15 Compositions and methods for using a solid support to purify rna
AU2004233035A AU2004233035B2 (en) 2001-10-12 2004-04-15 Compositions and methods for using a solid support to purify RNA

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/418,194 2003-04-16
US10/418,194 US7148343B2 (en) 2001-10-12 2003-04-16 Compositions and methods for using a solid support to purify RNA

Publications (2)

Publication Number Publication Date
WO2004094635A2 WO2004094635A2 (en) 2004-11-04
WO2004094635A3 true WO2004094635A3 (en) 2004-12-16

Family

ID=33309522

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/012033 WO2004094635A2 (en) 2001-10-12 2004-04-15 Compositions and methods for using a solid support to purify rna

Country Status (5)

Country Link
US (2) US7148343B2 (en)
EP (1) EP1618194B1 (en)
JP (2) JP4804347B2 (en)
CA (1) CA2522446C (en)
WO (1) WO2004094635A2 (en)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU9317198A (en) * 1997-09-17 1999-04-05 Gentra Systems, Inc. Apparatuses and methods for isolating nucleic acid
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
JP4633716B2 (en) * 2003-05-20 2011-02-16 インベスチゲン， インコーポレイテッド System for detecting polynucleotides
US20060281092A1 (en) * 2003-07-24 2006-12-14 Tanja Wille Method for the reverse transcription and/or amplification of nucleic acids
CA2549985C (en) * 2003-12-16 2011-05-24 Gentra Systems, Inc. Formulations and methods for denaturing proteins
WO2005093052A1 (en) * 2004-03-26 2005-10-06 Fuji Photo Film Co., Ltd. Method for selectively separating and purifying rna and method for separating and purifying nucleic acid
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
CA2560513A1 (en) * 2004-04-08 2005-12-01 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
WO2006052680A1 (en) * 2004-11-05 2006-05-18 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
EP1690938A1 (en) * 2005-02-11 2006-08-16 Qiagen GmbH Method for isolating nucleic acids, wherein the nucleic acids are immobilised on a matrix at elevated temperatures
DK1891232T3 (en) * 2005-06-14 2012-07-30 Statens Seruminstitut PCR diagnostics of dermatophytes and other pathogenic fungi
US20060286557A1 (en) * 2005-06-15 2006-12-21 Basehore Lee S Combined lysis and PCR buffer
US20070015165A1 (en) * 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
US20070092403A1 (en) * 2005-10-21 2007-04-26 Alan Wirbisky Compact apparatus, compositions and methods for purifying nucleic acids
US20070202511A1 (en) * 2006-02-28 2007-08-30 Sigma-Aldrich Co. Methods and compositions for the rapid isolation of small RNA molecules
GB0604973D0 (en) * 2006-03-11 2006-04-19 Central Science Lab Csl Of San Purification method and kit
KR100785010B1 (en) * 2006-04-06 2007-12-11 삼성전자주식회사 Method and apparatus for the purification of nucleic acids on hydrophilic surface of solid support using hydrogen bonding
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
US8080645B2 (en) 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US20130260369A1 (en) * 2012-03-28 2013-10-03 Longhorn Vaccines And Diagnostics, Llc Compositions and Methods for the Collection and Isolation of Nucleic Acids from Biological Specimens
WO2008071384A1 (en) * 2006-12-13 2008-06-19 Roche Diagnostics Gmbh Use of tde for the isolation of nucleic acids
EP2126074B1 (en) * 2006-12-13 2010-12-01 Roche Diagnostics GmbH Use of acetals for the isolation of nucleic acids
WO2008118566A2 (en) * 2007-02-28 2008-10-02 Ge Healthcare Bio-Sciences Corp. Ambient temperature stable chemical/biological reagents on membranes or filters
KR101443218B1 (en) * 2007-08-16 2014-09-24 삼성전자주식회사 Method for purifying RNA from biological material on solid support using kosmotropic salt
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US20090053704A1 (en) * 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
US9683256B2 (en) 2007-10-01 2017-06-20 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection and transport system
EP3058954B1 (en) 2007-08-27 2017-03-01 Longhorn Vaccines and Diagnostics, LLC Immunogenic compositions and methods
US10004799B2 (en) 2007-08-27 2018-06-26 Longhorn Vaccines And Diagnostics, Llc Composite antigenic sequences and vaccines
US8357296B2 (en) 2007-09-24 2013-01-22 Emd Millipore Corporation Centrifugal filter
ES2552858T3 (en) 2007-10-01 2015-12-02 Longhorn Vaccines And Diagnostics, Llc Collection of biological samples and transport system and methods of use
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
KR101432034B1 (en) * 2007-11-16 2014-08-21 삼성전자주식회사 Method for purifying small RNA from biological material on solid support using kosmotropic salts
US7871764B1 (en) * 2008-03-18 2011-01-18 The United States Of America As Represented By The United States Department Of Energy Universal nucleic acids sample preparation method for cells, spores and their mixture
TW201006517A (en) * 2008-05-22 2010-02-16 Asahi Kasei Medical Co Ltd Filtration method
EP2163620A1 (en) * 2008-09-03 2010-03-17 Qiagen GmbH Method for isolating and cleaning nucleic acids
DE102008047790A1 (en) * 2008-09-17 2010-04-15 Qiagen Gmbh Method for normalizing the content of biomolecules in a sample
EP2175020A1 (en) 2008-10-13 2010-04-14 Roche Diagnostics GmbH Reduction of RNase activity in complex fluidic samples
WO2010120874A2 (en) 2009-04-14 2010-10-21 Chimeros, Inc. Chimeric therapeutics, compositions, and methods for using same
WO2012018638A2 (en) * 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
US9255293B2 (en) * 2010-11-01 2016-02-09 Gen-Probe Incorporated Integrated capture and amplification of target nucleic acid for sequencing
GB201103363D0 (en) * 2011-02-28 2011-04-13 Ge Healthcare Uk Ltd Sample preservation method and sample application substrate
US9442046B2 (en) 2011-06-19 2016-09-13 Abogen, Inc. Device for sample collection
US9304070B2 (en) 2011-07-13 2016-04-05 Emd Millipore Corporation All-in-one sample preparation device and method
JP2014527072A (en) 2011-09-09 2014-10-09 バイオメド リアルティー， エル．ピー． Methods and compositions for controlling the assembly of viral proteins
EP3494989A1 (en) 2012-01-26 2019-06-12 Longhorn Vaccines and Diagnostics, LLC Composite antigenic sequences and vaccines
WO2013131083A1 (en) 2012-03-02 2013-09-06 Winthrop-University Hospital METHOD FOR USING PROBE BASED PCR DETECTION TO MEASURE THE LEVELS OF CIRCULATING DEMETHYLATED β CELL DERIVED DNA AS A MEASURE OF β CELL LOSS IN DIABETES
CN105143449B (en) * 2012-10-26 2018-11-13 株式会社钟化 RNA preparation methods
WO2014100755A2 (en) 2012-12-20 2014-06-26 Biomatrica, Inc. Formulations and methods for stabilizing pcr reagents
EP3114225B1 (en) 2014-03-07 2021-04-21 DNA Genotek Inc. Composition and method for stabilizing nucleic acids in biological samples
CN106572650B (en) 2014-06-10 2021-08-31 生物马特里卡公司 Stabilization of thrombocytes at ambient temperature
US10364428B2 (en) 2014-11-14 2019-07-30 Corning Incorporated Methods and kits for post-IVT RNA purification
US10968443B2 (en) * 2014-12-31 2021-04-06 The Rockefeller University Method of RNA isolation from clinical samples
EP3061822A1 (en) * 2015-02-25 2016-08-31 QIAGEN GmbH Method for concentrating, isolating and/or purifying nucleic acids from highly diluted aqueous samples
GB201504459D0 (en) * 2015-03-17 2015-04-29 Moorlodge Biotech Ventures Ltd Isolation of DNA
US9976136B2 (en) 2015-05-14 2018-05-22 Longhorn Vaccines And Diagnostics, Llc Rapid methods for the extraction of nucleic acids from biological samples
AU2016368265B2 (en) 2015-12-08 2021-10-28 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
GB201609115D0 (en) * 2016-05-24 2016-07-06 Moorlodge Biotech Ventures Ltd Isolating nucleic acids
US11112418B1 (en) * 2016-07-29 2021-09-07 Labrador Diagnostics Llc Systems and methods for multi-analysis
DK3568475T3 (en) 2017-01-16 2023-04-17 Spectrum Solutions L L C NUCLEIC ACID PRESERVATION SOLUTION AND METHODS OF USE
WO2018215375A1 (en) * 2017-05-22 2018-11-29 General Electric Company Improvements in the recovery of nucleic acids from solid supports
CN108130325A (en) * 2018-02-02 2018-06-08 苏州优卡新材料科技有限公司 A kind of efficiently purifying column of ribonucleic acid extraction inorganic material filter core
CN108456674A (en) * 2018-03-26 2018-08-28 深圳市展行生物有限公司 A kind of Total RNA extracts reagents and preparation method thereof
CN109343143A (en) * 2018-10-09 2019-02-15 中国地质科学院矿产资源研究所 Method for predicting concealed lithium deposit based on content of lithium element in plant
MX2021005762A (en) * 2018-11-14 2021-08-11 Spectrum Solutions L L C Rna preservation solution and methods of manufacture and use.
CA3120708A1 (en) 2018-11-20 2020-05-28 Spectrum Solutions, Llc Sample collection system including sealing cap and valve
US11701094B2 (en) 2019-06-20 2023-07-18 Spectrum Solutions L.L.C. Sample collection system including valve and plug assemblies
EP4077660A1 (en) * 2019-12-16 2022-10-26 QIAGEN GmbH Method for enriching vesicular rna
WO2023205281A1 (en) * 2022-04-20 2023-10-26 Siemens Healthcare Diagnostics Inc. Quantitation of alkylphenol ethoxylate compounds in aqueous samples

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034569A1 (en) * 1994-06-14 1995-12-21 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
EP0818461A2 (en) * 1996-07-12 1998-01-14 Toyo Boseki Kabushiki Kaisha Method for isolating ribonucleic acid
US20030073830A1 (en) * 2001-10-12 2003-04-17 Heath Ellen M. Compositions and methods for using a solid support to purify RNA
WO2003033739A1 (en) * 2001-10-12 2003-04-24 Gentra Systems, Inc. Compositions and methods for using a solid support to purify rna

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4483920A (en) * 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
DE3639949A1 (en) 1986-11-22 1988-06-09 Diagen Inst Molekularbio METHOD FOR SEPARATING LONG CHAIN NUCLEIC ACIDS
US5599667A (en) 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
US4843155A (en) 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5010183A (en) 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
DE4034036C2 (en) 1990-10-26 1994-03-03 Diagen Inst Molekularbio Device and method for isolating nucleic acids from cell suspensions
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US5155018A (en) 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
JP3615545B2 (en) 1993-02-01 2005-02-02 キアゲン・エヌ・ブイ Quaternary ammonium salt surfactant and its RNA isolator
DE4321904B4 (en) 1993-07-01 2013-05-16 Qiagen Gmbh Method for chromatographic purification and separation of nucleic acid mixtures
GB9314249D0 (en) 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
US5637687A (en) * 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
US6037465A (en) 1994-06-14 2000-03-14 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
AU5296496A (en) * 1995-03-24 1996-10-16 Mitokor Mutation detection by differential primer extension of mutant and wildtype target sequences
US5945515A (en) 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US5973137A (en) 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
JP3082908B2 (en) * 1996-07-12 2000-09-04 東洋紡績株式会社 Method for isolating ribonucleic acid
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
JP2002507121A (en) * 1997-06-25 2002-03-05 プロメガ コーポレイション RNA isolation method
US5958677A (en) 1997-07-28 1999-09-28 The New York Blood Center, Inc. Method for purifying viral nucleic acids
US6207370B1 (en) * 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
AU9317198A (en) * 1997-09-17 1999-04-05 Gentra Systems, Inc. Apparatuses and methods for isolating nucleic acid
US5972613A (en) 1997-12-09 1999-10-26 The Perkin-Elmer Corporation Methods of nucleic acid isolation
CA2315257A1 (en) * 1997-12-18 1999-07-01 Invitek Gmbh Method for isolating short and long-chain nucleic acids
JPH11196869A (en) * 1998-01-19 1999-07-27 Toyobo Co Ltd Isolation of liponucleic acid
ZA99493B (en) * 1998-01-30 1999-07-22 Akzo Nobel Nv Method for the isolation of nucleic acid.
AU2654899A (en) 1998-02-02 1999-08-16 Gentra Systems, Inc. Processes for isolating, amplifying and characterizing dna
US6090593A (en) * 1998-05-13 2000-07-18 The United States Of America As Represented By The Secretary Of The Air Force Isolation of expressed genes in microorganisms
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
JP3183458B2 (en) * 1998-08-12 2001-07-09 農林水産省国際農林水産業研究センター所長 Genes encoding plant transcription factors
US6777210B1 (en) * 1998-09-24 2004-08-17 Ambion, Inc. Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
DE19856064C2 (en) 1998-12-04 2000-11-30 Invitek Gmbh Universal method for the isolation of DNA from any starting material
US6270970B1 (en) * 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US20060276629A9 (en) * 1999-12-17 2006-12-07 Hildebrand William H Purification and characterization of soluble human HLA proteins
JP3922420B2 (en) * 2000-04-18 2007-05-30 東洋紡績株式会社 Improved method for isolating ribonucleic acid
US6919307B2 (en) * 2000-11-01 2005-07-19 Praecis Pharmaceuticals, Inc. Therapeutic agents and methods of use thereof for the modulation of angiogenesis
US6602718B1 (en) * 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
US6503716B1 (en) * 2000-11-28 2003-01-07 Pe Corporation (Ny) Compositions and methods for extracting a nucleic acid
US6818398B2 (en) * 2000-12-29 2004-11-16 The University Of Chicago Column device for isolation and labeling of nucleic acids
EP1349951A4 (en) * 2001-01-09 2005-08-24 Whitehead Biomedical Inst Methods and reagents for the isolation of nucleic acids
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
WO2003040687A2 (en) * 2001-11-06 2003-05-15 Cortex Biochem, Inc. Isolation and purification of nucleic acids
JP4220164B2 (en) * 2002-02-06 2009-02-04 東洋紡績株式会社 Nucleic acid purification method, nucleic acid extraction solution used in the method, and nucleic acid purification reagent kit
DE10231659B4 (en) * 2002-07-12 2006-01-19 Preanalytix Gmbh A composition for binding nucleic acid to a solid phase
GB0225197D0 (en) * 2002-10-30 2002-12-11 Univ Sheffield Surface
DE60323153D1 (en) * 2002-12-20 2008-10-02 Du Pont DIAGNOSTIC SEQUENCES FOR MALE AND CLAUSE DISEASE
GB0230102D0 (en) * 2002-12-23 2003-01-29 Spinox Ltd Apparatus and method for storing proteins
EP1631668A1 (en) * 2003-06-06 2006-03-08 Applera Corporation Purification device for ribonucleic acid in large volumes, and method
CA2549985C (en) * 2003-12-16 2011-05-24 Gentra Systems, Inc. Formulations and methods for denaturing proteins
WO2006052680A1 (en) * 2004-11-05 2006-05-18 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
KR100691372B1 (en) * 2005-10-19 2007-03-12 삼성전기주식회사 Electrowetting system comprising conducting liquid with reliability
US20070092403A1 (en) * 2005-10-21 2007-04-26 Alan Wirbisky Compact apparatus, compositions and methods for purifying nucleic acids
US20070141583A1 (en) * 2005-12-20 2007-06-21 Weiwei Li Methods of rapid chromatin immunoprecipitation
US7538190B2 (en) * 2006-02-17 2009-05-26 Polychip Pharmaceuticals Pty Ltd Methods for the synthesis of two or more dicarba bridges in organic compounds
WO2007149791A2 (en) * 2006-06-15 2007-12-27 Stratagene System for isolating biomolecules from a sample

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034569A1 (en) * 1994-06-14 1995-12-21 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
EP0818461A2 (en) * 1996-07-12 1998-01-14 Toyo Boseki Kabushiki Kaisha Method for isolating ribonucleic acid
US20030073830A1 (en) * 2001-10-12 2003-04-17 Heath Ellen M. Compositions and methods for using a solid support to purify RNA
WO2003033739A1 (en) * 2001-10-12 2003-04-24 Gentra Systems, Inc. Compositions and methods for using a solid support to purify rna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DAHLE C E ET AL: "ISOLATION OF RNA FROM CELLS IN CULTURE USING CATRIMOX-14TM CATIONIC SURFACTANT", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 15, no. 6, December 1993 (1993-12-01), pages 1102 - 1105, XP001055650, ISSN: 0736-6205 *

Also Published As

Publication number Publication date
EP1618194B1 (en) 2016-11-23
CA2522446A1 (en) 2004-11-04
US20040019196A1 (en) 2004-01-29
JP2010263919A (en) 2010-11-25
WO2004094635A2 (en) 2004-11-04
CA2522446C (en) 2013-09-24
JP2006523463A (en) 2006-10-19
US7767804B2 (en) 2010-08-03
US7148343B2 (en) 2006-12-12
US20070043216A1 (en) 2007-02-22
EP1618194A2 (en) 2006-01-25
JP4804347B2 (en) 2011-11-02

Similar Documents

Publication Publication Date Title
WO2004094635A3 (en) Compositions and methods for using a solid support to purify rna
AU2003900368A0 (en) Assay for nucleic acid molecules
WO2004037988A3 (en) Methods for synthesizing complementary dna
WO2005087951A3 (en) Reagents, methods and kits for use in deactivating nucleic acids
EP2295567A3 (en) Compositions and methods for using a solid support to purify DNA
AU2003254334A1 (en) Rna-interference by single-stranded rna molecules
DE60330399D1 (en) TRICHMICROSCOPY, CULTURE AND POLYMERASE CHAIN REACTION USING PROCESSED CLINICAL SAMPLES AND KIT THEREOF
AU2003299541A1 (en) Microfluidic nucleic acid analysis
WO2006023471A3 (en) Additive, method, and article for dna collection, stabilization, and purification
AU4332399A (en) Rna polymerase chain reaction
WO2005047545A3 (en) Microarray controls
AU2002314997A1 (en) Methods for low background cloning of dna using long oligonucleotides
AUPS076902A0 (en) Novel selective polymerase chain reaction
ATE344321T1 (en) METHOD FOR THE PURIFICATION OF BACTERIAL CELLS AND CELL COMPONENTS
WO2005001039A3 (en) Ribozyme-regulated small inhibitory rna (sirna) production and methods of use thereof
WO2007035685A3 (en) Compositions and methods for purifying nucleic acids
AU2003257133A1 (en) Methods, devices and kits for multiplex blotting of biological samples from multi-well plates
AU2003276765A1 (en) Method for amplifying unknown dna sequence adjacent to known sequence
PT2258847T (en) Futher novel forms of interfering rna molecules
AU2003232036A1 (en) Nanoarchaeum nucleic acid molecule
AU2003210073A1 (en) Membrane bioreactor
WO2005004606A3 (en) Pyridinylanilides
AU2002310296A1 (en) Rna purification methods
AU2002250066A1 (en) Confirming the exon content of rna transcripts by pcr using primers complementary to each respective exon
AU2003267907A1 (en) Prothrombin purification

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2004233035

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2006513124

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2522446

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2004233035

Country of ref document: AU

Date of ref document: 20040415

Kind code of ref document: A

WWP Wipo information: published in national office

Ref document number: 2004233035

Country of ref document: AU

REEP Request for entry into the european phase

Ref document number: 2004760008

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2004760008

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2004760008

Country of ref document: EP