WO2004072279A3 - Proteases - Google Patents

Proteases Download PDF

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Publication number
WO2004072279A3
WO2004072279A3 PCT/DK2004/000091 DK2004000091W WO2004072279A3 WO 2004072279 A3 WO2004072279 A3 WO 2004072279A3 DK 2004000091 W DK2004000091 W DK 2004000091W WO 2004072279 A3 WO2004072279 A3 WO 2004072279A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteases
relates
brachysporiella
sub
metarhizium
Prior art date
Application number
PCT/DK2004/000091
Other languages
French (fr)
Other versions
WO2004072279A2 (en
Inventor
Katrine Pontoppidan
Wenping Wu
Tang Lan
Ye Liu
Maria Leonardo De
Original Assignee
Novozymes As
Katrine Pontoppidan
Wenping Wu
Tang Lan
Ye Liu
Maria Leonardo De
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As, Katrine Pontoppidan, Wenping Wu, Tang Lan, Ye Liu, Maria Leonardo De filed Critical Novozymes As
Publication of WO2004072279A2 publication Critical patent/WO2004072279A2/en
Publication of WO2004072279A3 publication Critical patent/WO2004072279A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

The invention relates to a sub-class of novel proteases with distinct differences of expected structural relevance to known proteases of Metarhizium and Nocardiopsis. The sub-class encompasses proteases derived from Gliocladium, Periconia, Curvularia, and Brachysporiella. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g. in animal feed and in detergents. The invention furthermore relates to the use in animal feed of proteases of at least 55% identity to the protease derived from Brachysporiella. The Metarhizium protease is an The invention relates to a sub-class of novel proteases with distinct differences of expected structural relevance to known proteases of Metarhizium and Nocardiopsis. The sub-class encompasses proteases derived from Gliocladium, Periconia, Curvularia, and Brachysporiella. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g. in animal feed and in detergents. The invention furthermore relates to the use in animal feed of proteases of at least 55% identity to the protease derived from Brachysporiella. The Metarhizium protease is an example of such proteases.
PCT/DK2004/000091 2003-02-07 2004-02-09 Proteases WO2004072279A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200300173 2003-02-07
DKPA200300173 2003-02-07
DKPA200300426 2003-03-20
DKPA200300426 2003-03-20

Publications (2)

Publication Number Publication Date
WO2004072279A2 WO2004072279A2 (en) 2004-08-26
WO2004072279A3 true WO2004072279A3 (en) 2004-10-28

Family

ID=32870662

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2004/000091 WO2004072279A2 (en) 2003-02-07 2004-02-09 Proteases

Country Status (1)

Country Link
WO (1) WO2004072279A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8865449B2 (en) 2003-11-19 2014-10-21 Danisco Us Inc. Multiple mutation variants of serine protease
CN107446033B (en) * 2006-02-22 2022-04-01 朱正崙 Treatment of developmental related disorders

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6855548B2 (en) 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
DE602004007766T2 (en) 2003-02-07 2008-06-05 Novozymes A/S PROTEASE
EP1639107B1 (en) * 2003-06-19 2013-08-14 Novozymes A/S Improved proteases and methods for producing them
ATE463563T1 (en) 2003-06-19 2010-04-15 Novozymes As IMPROVED PROTEASES AND METHODS FOR THE PRODUCTION THEREOF
EP1673451B1 (en) * 2003-10-10 2010-12-15 Novozymes A/S Protease variants
EP1694847B1 (en) * 2003-11-19 2012-06-13 Danisco US Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
JP5623691B2 (en) 2004-06-21 2014-11-12 ノボザイムスアクティーゼルスカブ Protease
JP5421117B2 (en) 2006-12-22 2014-02-19 ノボザイムス アクティーゼルスカブ Process for producing yeast extract
PL2300606T3 (en) 2008-06-03 2012-05-31 Novozymes As Method for producing a casein hydrolysate
WO2010112546A1 (en) 2009-04-02 2010-10-07 Novozymes A/S Process for making a milk-based protein hydrolysate
EP2436389A1 (en) 2010-10-01 2012-04-04 Nestec S.A. Milk-based protein hydrolysates and infant formulae and nutritional compositions made thereof
CA2809916A1 (en) 2010-10-01 2012-04-05 Novozymes A/S Polypeptides having endopeptidase activity and polynucleotides encoding same
US20140295027A1 (en) 2011-08-19 2014-10-02 Novozymes A/S Polypeptides Having Protease Activity
ES2628190T3 (en) * 2011-09-22 2017-08-02 Novozymes A/S Polypeptides with protease activity and polynucleotides encoding them
CN104024408B (en) * 2011-12-28 2019-04-19 诺维信公司 Polypeptide with proteinase activity
EP2807254B1 (en) * 2012-01-26 2017-08-02 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
MX364390B (en) 2012-06-20 2019-04-25 Novozymes As Use of polypeptides having protease activity in animal feed and detergents.
CN104884615B (en) 2012-09-05 2019-07-12 诺维信公司 Polypeptide with proteinase activity
FR2998450A1 (en) * 2012-11-26 2014-05-30 Dominique Hooreman USE OF AN ENZYMATIC COMPLEX IN FEEDING ANIMAL BREEDING ANIMALS
ES2655032T3 (en) 2012-12-21 2018-02-16 Novozymes A/S Polypeptides that possess protease activity and polynucleotides that encode them
WO2014122161A2 (en) 2013-02-06 2014-08-14 Novozymes A/S Polypeptides having protease activity
MX2017005643A (en) 2014-11-04 2017-08-07 Novozymes As Polypeptides having serine protease activity and polynucleotides encoding same and their application in animal feed.
WO2017080511A1 (en) * 2015-11-12 2017-05-18 Novozymes A/S Agitation, aeration and /or fermentation processes with reduced foam
EP3596210A1 (en) * 2017-03-15 2020-01-22 DuPont Nutrition Biosciences ApS Trypsin-like serine proteases and uses thereof cross-reference to related application

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0300466A1 (en) * 1987-07-21 1989-01-25 Cangene Corporation Characterization and structure of genes for protease A and protease B from streptomyces griseus
EP0647710A1 (en) * 1992-05-19 1995-04-12 Shionogi Seiyaku Kabushiki Kaisha Novel protease
US5646028A (en) * 1991-06-18 1997-07-08 The Clorox Company Alkaline serine protease streptomyces griseus var. alkaliphus having enhanced stability against urea or guanidine
WO2001058276A2 (en) * 2000-02-08 2001-08-16 F Hoffmann-La Roche Ag Use of acid-stable proteases in animal feed

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0300466A1 (en) * 1987-07-21 1989-01-25 Cangene Corporation Characterization and structure of genes for protease A and protease B from streptomyces griseus
US5646028A (en) * 1991-06-18 1997-07-08 The Clorox Company Alkaline serine protease streptomyces griseus var. alkaliphus having enhanced stability against urea or guanidine
EP0647710A1 (en) * 1992-05-19 1995-04-12 Shionogi Seiyaku Kabushiki Kaisha Novel protease
WO2001058276A2 (en) * 2000-02-08 2001-08-16 F Hoffmann-La Roche Ag Use of acid-stable proteases in animal feed

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Alignment for peptidase family S1E", MEROPS DATABASE, 26 March 2004 (2004-03-26), XP002285393, Retrieved from the Internet <URL:http://www.merops.ac.uk/famcards/aln/s1e_fra.htm> [retrieved on 20040621] *
SCREEN S E ET AL: "Cloning, expression, and substrate specificity of a fungal chymotrypsin. Evidence for lateral gene transfer from an actinomycete bacterium.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. 3 MAR 2000, vol. 275, no. 9, 3 March 2000 (2000-03-03), pages 6689 - 6694, XP002285828, ISSN: 0021-9258 *
SIDHU S S ET AL: "Streptomyces griseus protease C. A novel enzyme of the chymotrypsin superfamily.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. 5 AUG 1994, vol. 269, no. 31, 5 August 1994 (1994-08-05), pages 20167 - 20171, XP002285827, ISSN: 0021-9258 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8865449B2 (en) 2003-11-19 2014-10-21 Danisco Us Inc. Multiple mutation variants of serine protease
CN107446033B (en) * 2006-02-22 2022-04-01 朱正崙 Treatment of developmental related disorders

Also Published As

Publication number Publication date
WO2004072279A2 (en) 2004-08-26

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