WO2004063700A3 - Multiplex analytical platform using molecular tags - Google Patents

Multiplex analytical platform using molecular tags Download PDF

Info

Publication number
WO2004063700A3
WO2004063700A3 PCT/US2003/039613 US0339613W WO2004063700A3 WO 2004063700 A3 WO2004063700 A3 WO 2004063700A3 US 0339613 W US0339613 W US 0339613W WO 2004063700 A3 WO2004063700 A3 WO 2004063700A3
Authority
WO
WIPO (PCT)
Prior art keywords
template
molecular tags
extension reaction
target analytes
dependent extension
Prior art date
Application number
PCT/US2003/039613
Other languages
French (fr)
Other versions
WO2004063700A2 (en
Inventor
Tracy Matray
Sharat Singh
Stephen C Macevicz
Original Assignee
Aclara Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aclara Biosciences Inc filed Critical Aclara Biosciences Inc
Priority to EP03815205A priority Critical patent/EP1581796A4/en
Priority to AU2003296989A priority patent/AU2003296989A1/en
Priority to JP2004566540A priority patent/JP2006518587A/en
Publication of WO2004063700A2 publication Critical patent/WO2004063700A2/en
Publication of WO2004063700A3 publication Critical patent/WO2004063700A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Abstract

Compositions and methods are disclosed for detecting multiple target analytes, particularly polynucleotide target analytes. In accordance with one aspect of the invention, a template-dependent extension reaction is performed to generate detection probes, such that each detection probe has (i) at least one molecular tag attached by a cleavable linkage and (ii) either a capture moiety or a cleavage-inducing moiety attached. The template-dependent extension reaction may be carried out directly on a polynucleotide analyte to generate molecular tags, wherein the polynucleotide analyte serves as a template in the template-dependent extension reaction, or it may be carried out indirectly on an oligonucleotide label that, in turn, is attached to a binding moiety specific for an analyte of interest. In either case, a plurality of molecular tags are generated, after which they are separated and identified to determine the presence or absence or the quantity of the target analytes in a sample.
PCT/US2003/039613 2003-01-07 2003-12-12 Multiplex analytical platform using molecular tags WO2004063700A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP03815205A EP1581796A4 (en) 2003-01-07 2003-12-12 Multiplex analytical platform using molecular tags
AU2003296989A AU2003296989A1 (en) 2003-01-07 2003-12-12 Multiplex analytical platform using molecular tags
JP2004566540A JP2006518587A (en) 2003-01-07 2003-12-12 Multiple analysis platform using molecular tags

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/338,729 US20030207300A1 (en) 2000-04-28 2003-01-07 Multiplex analytical platform using molecular tags
US10/338,729 2003-01-07

Publications (2)

Publication Number Publication Date
WO2004063700A2 WO2004063700A2 (en) 2004-07-29
WO2004063700A3 true WO2004063700A3 (en) 2006-01-05

Family

ID=32710992

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/039613 WO2004063700A2 (en) 2003-01-07 2003-12-12 Multiplex analytical platform using molecular tags

Country Status (5)

Country Link
US (1) US20030207300A1 (en)
EP (1) EP1581796A4 (en)
JP (1) JP2006518587A (en)
AU (1) AU2003296989A1 (en)
WO (1) WO2004063700A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44437E1 (en) 2003-04-01 2013-08-13 Monogram Biosciences, Inc. Methods for detecting receptor complexes comprising PI3K
US9110066B2 (en) 2009-01-15 2015-08-18 Laboratory Corporation Of America Holdings HER-3 antibodies and methods of use

Families Citing this family (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE504798C2 (en) * 1995-06-16 1997-04-28 Ulf Landegren Immunoassay and test kits with two reagents that can be cross-linked if adsorbed to the analyte
US7402398B2 (en) 2003-07-17 2008-07-22 Monogram Biosciences, Inc. Measuring receptor homodimerization
JP2007502417A (en) * 2003-08-11 2007-02-08 モノグラム バイオサイエンシズ，インコーポレーテッド Detection and characterization of molecular complexes
WO2005044836A2 (en) * 2003-11-05 2005-05-19 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
EP1713936B1 (en) * 2004-02-12 2009-12-09 Population Genetics Technologies Ltd Corporation of Great Britain Genetic analysis by sequence-specific sorting
WO2005085850A1 (en) * 2004-02-27 2005-09-15 Tianxin Wang Methods for multiplexed analyte detection
CN101087890A (en) * 2004-07-26 2007-12-12 帕拉列勒生物科学公司 Simultaneous analysis of multiple genomes
US7407757B2 (en) * 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
WO2006086209A2 (en) * 2005-02-10 2006-08-17 Compass Genetics, Llc Genetic analysis by sequence-specific sorting
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
WO2006099604A2 (en) * 2005-03-16 2006-09-21 Compass Genetics, Llc Methods and compositions for assay readouts on multiple analytical platforms
EP1885876A2 (en) * 2005-03-17 2008-02-13 Genovoxx GmbH Macromolecular nucleotide links and methods for their use
US20060281104A1 (en) * 2005-06-13 2006-12-14 Macevicz Stephen C Leuco dye particles and uses thereof
WO2007044903A2 (en) * 2005-10-11 2007-04-19 Stratagene California Binary signal detection assays
US7537897B2 (en) * 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
EP2074133A2 (en) * 2006-09-20 2009-07-01 Genovoxx GmbH Components and method for enzymatic synthesis of nucleic acids
CA2706763C (en) 2007-11-27 2013-07-30 Laboratory Corporation Of America Holdings Enhanced method for detecting and/or quantifying an analyte in a sample
US10416162B2 (en) 2007-12-20 2019-09-17 Monogram Biosciences, Inc. Her2 diagnostic methods
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
WO2010053587A2 (en) 2008-11-07 2010-05-14 Mlc Dx Incorporated Methods of monitoring conditions by sequence analysis
US8309306B2 (en) * 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
CA2764386C (en) 2008-12-01 2018-05-01 Laboratory Corporation Of America Holdings P95-her2 antibodies and uses thereof
KR20110120890A (en) * 2009-01-15 2011-11-04 래버러토리 코포레이션 오브 아메리카 홀딩스 Methods of determining patient response by measurement of her-2 expression
EP2387627B1 (en) 2009-01-15 2016-03-30 Adaptive Biotechnologies Corporation Adaptive immunity profiling and methods for generation of monoclonal antibodies
RU2539032C2 (en) 2009-06-25 2015-01-10 Фред Хатчинсон Кансэр Рисёч Сентер Method for measuring artificial immunity
WO2011021102A2 (en) 2009-08-20 2011-02-24 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
EP2623613B8 (en) 2010-09-21 2016-09-07 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
EP2768982A4 (en) 2011-10-21 2015-06-03 Adaptive Biotechnologies Corp Quantification of adaptive immune cell genomes in a complex mixture of cells
AU2012347460B2 (en) 2011-12-09 2017-05-25 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
ES2663234T3 (en) 2012-02-27 2018-04-11 Cellular Research, Inc Compositions and kits for molecular counting
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
US9670529B2 (en) 2012-02-28 2017-06-06 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
WO2013134162A2 (en) 2012-03-05 2013-09-12 Sequenta, Inc. Determining paired immune receptor chains from frequency matched subunits
US9862995B2 (en) 2012-03-13 2018-01-09 Abhijit Ajit Patel Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
SG10201507700VA (en) 2012-05-08 2015-10-29 Adaptive Biotechnologies Corp Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
KR102210852B1 (en) 2012-09-04 2021-02-01 가던트 헬쓰, 인크. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN105189779B (en) 2012-10-01 2018-05-11 适应生物技术公司 The immunocompetence carried out by adaptive immunity receptor diversity and Clonal characterization is assessed
US10150996B2 (en) 2012-10-19 2018-12-11 Adaptive Biotechnologies Corp. Quantification of adaptive immune cell genomes in a complex mixture of cells
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
RU2670133C2 (en) 2012-11-19 2018-10-18 Аптон Биосистемс, Инк. Digital analysis of molecular analytes using single molecule detection
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
JP6585046B2 (en) 2013-08-22 2019-10-02 アプトン バイオシステムズ インコーポレイテッド Method for digital analysis of molecular analytes using electrical methods
EP3039158B1 (en) 2013-08-28 2018-11-14 Cellular Research, Inc. Massively parallel single cell analysis
JP2017504307A (en) 2013-10-07 2017-02-09 セルラー リサーチ， インコーポレイテッド Method and system for digitally counting features on an array
SG11201604923XA (en) 2013-12-28 2016-07-28 Guardant Health Inc Methods and systems for detecting genetic variants
AU2015227054A1 (en) 2014-03-05 2016-09-22 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
GB201414451D0 (en) * 2014-08-14 2014-10-01 Oxford Gene Technology Operations Ltd Hybridisation column for nucleic acid enrichment
AU2015339191A1 (en) 2014-10-29 2017-05-18 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
EP3498866A1 (en) 2014-11-25 2019-06-19 Adaptive Biotechnologies Corp. Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
US10697010B2 (en) 2015-02-19 2020-06-30 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
US11047008B2 (en) 2015-02-24 2021-06-29 Adaptive Biotechnologies Corporation Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP3277294A4 (en) 2015-04-01 2018-11-14 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
ES2745694T3 (en) 2015-09-11 2020-03-03 Cellular Res Inc Methods and compositions for nucleic acid library normalization
CN108603228B (en) 2015-12-17 2023-09-01 夸登特健康公司 Method for determining tumor gene copy number by analyzing cell-free DNA
JP2019515252A (en) 2016-03-15 2019-06-06 ラボラトリー コーポレイション オブ アメリカ ホールディングス Method of evaluating protein interaction between cells
ES2956757T3 (en) 2016-05-02 2023-12-27 Becton Dickinson Co Accurate molecular barcode coding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US11397882B2 (en) 2016-05-26 2022-07-26 Becton, Dickinson And Company Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
KR102363716B1 (en) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. Determination of protein expression using reagents having barcoded oligonucleotide sequences
EP3539035B1 (en) 2016-11-08 2024-04-17 Becton, Dickinson and Company Methods for expression profile classification
SG11201903158RA (en) 2016-11-08 2019-05-30 Cellular Res Inc Methods for cell label classification
EP3568234B1 (en) 2017-01-13 2023-09-06 Cellular Research, Inc. Hydrophilic coating of fluidic channels
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
JP7305611B2 (en) 2017-03-17 2023-07-10 アプトン バイオシステムズ インコーポレイテッド Methods of sequencing and high-resolution imaging
JP2020522262A (en) 2017-06-05 2020-07-30 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ Sample index addition for single cells
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
EP3728636A1 (en) 2017-12-19 2020-10-28 Becton, Dickinson and Company Particles associated with oligonucleotides
CN112243461A (en) 2018-05-03 2021-01-19 贝克顿迪金森公司 Molecular barcoding at opposite transcript ends
EP4234717A3 (en) 2018-05-03 2023-11-01 Becton, Dickinson and Company High throughput multiomics sample analysis
CN112805389A (en) 2018-10-01 2021-05-14 贝克顿迪金森公司 Determination of 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
CN115605614A (en) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） Primers for immune repertoire profiling
US11891643B2 (en) 2020-06-03 2024-02-06 Siphox, Inc. Methods and systems for monomer chain formation
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470705A (en) * 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
US5876936A (en) * 1997-01-15 1999-03-02 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators
US6001573A (en) * 1996-06-14 1999-12-14 Packard Bioscience B.V. Use of porphyrins as a universal label

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4331590A (en) * 1978-03-13 1982-05-25 Miles Laboratories, Inc. β-Galactosyl-umbelliferone-labeled protein and polypeptide conjugates
FR2431581A1 (en) * 1978-07-18 1980-02-15 Soum Rene FLOOR ASSEMBLY SYSTEM
US4650750A (en) * 1982-02-01 1987-03-17 Giese Roger W Method of chemical analysis employing molecular release tag compounds
US4709016A (en) * 1982-02-01 1987-11-24 Northeastern University Molecular analytical release tags and their use in chemical analysis
US5516931A (en) * 1982-02-01 1996-05-14 Northeastern University Release tag compounds producing ketone signal groups
US5650270A (en) * 1982-02-01 1997-07-22 Northeastern University Molecular analytical release tags and their use in chemical analysis
GB8407736D0 (en) * 1984-03-26 1984-05-02 Univ London Detecting specific polynucleotide sequences
US4675300A (en) * 1985-09-18 1987-06-23 The Board Of Trustees Of The Leland Stanford Junior University Laser-excitation fluorescence detection electrokinetic separation
US4780421A (en) * 1986-04-03 1988-10-25 Sclavo Inc. Cleavable labels for use in binding assays
US5494793A (en) * 1986-12-15 1996-02-27 British Technology Group Usa Inc. Monomeric phthalocyanine reagents
AU636110B2 (en) * 1988-06-08 1993-04-22 Nichols Institute Diagnostics Assays utilizing sensitizer-induced production of detectable signal
US5571666A (en) * 1988-10-28 1996-11-05 Oklahoma Medical Research Foundation Thiazine dyes used to inactivate HIV in biological fluids
US5254469A (en) * 1989-09-12 1993-10-19 Eastman Kodak Company Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures
US5578498A (en) * 1991-05-22 1996-11-26 Behringwerke Ag Metal chelate containing compositions for use in chemiluminescent assays
US5340716A (en) * 1991-06-20 1994-08-23 Snytex (U.S.A.) Inc. Assay method utilizing photoactivated chemiluminescent label
US6251581B1 (en) * 1991-05-22 2001-06-26 Dade Behring Marburg Gmbh Assay method utilizing induced luminescence
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
EP0566751B1 (en) * 1992-03-23 1996-01-10 F. Hoffmann-La Roche Ag DNA detection method
ATE173767T1 (en) * 1992-04-03 1998-12-15 Perkin Elmer Corp SAMPLES COMPOSITION AND METHODS
ES2113547T3 (en) * 1992-07-31 1998-05-01 Behringwerke Ag PHOTOACTIVABLE CHEMIOLUMINISCENT MATRICES.
US5958202A (en) * 1992-09-14 1999-09-28 Perseptive Biosystems, Inc. Capillary electrophoresis enzyme immunoassay
US5721099A (en) * 1992-10-01 1998-02-24 Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5565324A (en) * 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5719028A (en) * 1992-12-07 1998-02-17 Third Wave Technologies Inc. Cleavase fragment length polymorphism
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
US5635602A (en) * 1993-08-13 1997-06-03 The Regents Of The University Of California Design and synthesis of bispecific DNA-antibody conjugates
CA2170873A1 (en) * 1993-09-03 1995-03-09 Dariush Davalian Fluorescent oxygen channeling immunoassays
CN1525171A (en) * 1993-10-01 2004-09-01 ŦԼ�и��ױ��Ǵ�ѧ���� Complex combinatorial chemical libraries encoded with tags
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
AU1254295A (en) * 1993-12-17 1995-07-03 Perkin-Elmer Corporation, The Uncharged polymers for separation of biomolecules by capillary electrophoresis
US5851770A (en) * 1994-04-25 1998-12-22 Variagenics, Inc. Detection of mismatches by resolvase cleavage using a magnetic bead support
US5986076A (en) * 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US6045995A (en) * 1994-08-24 2000-04-04 Isis Pharmaceuticals, Inc. Capillary electrophoretic detection of nucleic acids
US5843666A (en) * 1994-09-02 1998-12-01 Lumigen, Inc. Chemiluminescent detection methods using dual enzyer-labeled binding partners
US5691151A (en) * 1994-10-07 1997-11-25 Regents Of University Of California Methods of screening for ulcerative colitis and crohn's disease by detecting VH3-15 autoantibody and panca
US5560811A (en) * 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method
WO1996031263A1 (en) * 1995-04-06 1996-10-10 Arqule, Inc. Method for rapid purification, analysis and characterization of collections of chemical compounds
US5591422A (en) * 1995-06-02 1997-01-07 Pharmacyclics, Inc. Texaphyrin complexes having improved functionalization
US5843655A (en) * 1995-09-18 1998-12-01 Affymetrix, Inc. Methods for testing oligonucleotide arrays
US5846839A (en) * 1995-12-22 1998-12-08 Glaxo Group Limited Methods for hard-tagging an encoded synthetic library
US6312893B1 (en) * 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6090947A (en) * 1996-02-26 2000-07-18 California Institute Of Technology Method for the synthesis of pyrrole and imidazole carboxamides on a solid support
US5811239A (en) * 1996-05-13 1998-09-22 Frayne Consultants Method for single base-pair DNA sequence variation detection
US6780982B2 (en) * 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
US5998140A (en) * 1996-07-31 1999-12-07 The Scripps Research Institute Complex formation between dsDNA and oligomer of cyclic heterocycles
US6045676A (en) * 1996-08-26 2000-04-04 The Board Of Regents Of The University Of California Electrochemical detector integrated on microfabricated capilliary electrophoresis chips
US5763602A (en) * 1996-10-01 1998-06-09 Li; Ying-Syi Methods of syntheses of phthalocyanine compounds
US5952654A (en) * 1997-10-29 1999-09-14 Northeastern University Field-release mass spectrometry
US6562567B2 (en) * 1998-01-27 2003-05-13 California Institute Of Technology Method of detecting a nucleic acid
US6335201B1 (en) * 1998-03-06 2002-01-01 The Regents Of The University Of California Method and apparatus for detecting enzymatic activity using molecules that change electrophoretic mobility
US6740497B2 (en) * 1998-03-06 2004-05-25 The Regents Of The University Of California Method and apparatus for detecting cancerous cells using molecules that change electrophoretic mobility
US6322980B1 (en) * 1999-04-30 2001-11-27 Aclara Biosciences, Inc. Single nucleotide detection using degradation of a fluorescent sequence
US6331530B1 (en) * 1999-07-13 2001-12-18 The Trustees Of Columbia University In The City Of New York Hydrophilic carrier for photosensitizers that cleaves when they catalyze the formation of singlet oxygen
US6346384B1 (en) * 2000-03-27 2002-02-12 Dade Behring Inc. Real-time monitoring of PCR using LOCI
ATE423221T1 (en) * 2000-06-13 2009-03-15 Univ Boston USE OF MASS-MATCHED NUCLEOTIDES IN THE ANALYSIS OF OLIGONUCLEOTIDE MIXTURES AND IN HIGH-MULTIPLEX NUCLEIC ACID SEQUENCING
WO2002029109A2 (en) * 2000-10-05 2002-04-11 Aclara Biosciences, Inc. Multiplexed differential displacement for nucleic acid determinations
US6743905B2 (en) * 2001-04-16 2004-06-01 Applera Corporation Mobility-modified nucleobase polymers and methods of using same
US7358052B2 (en) * 2001-05-26 2008-04-15 Monogram Biosciences, Inc. Catalytic amplification of multiplexed assay signals

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470705A (en) * 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
US6001573A (en) * 1996-06-14 1999-12-14 Packard Bioscience B.V. Use of porphyrins as a universal label
US5876936A (en) * 1997-01-15 1999-03-02 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44437E1 (en) 2003-04-01 2013-08-13 Monogram Biosciences, Inc. Methods for detecting receptor complexes comprising PI3K
US9110066B2 (en) 2009-01-15 2015-08-18 Laboratory Corporation Of America Holdings HER-3 antibodies and methods of use

Also Published As

Publication number Publication date
AU2003296989A1 (en) 2004-08-10
EP1581796A2 (en) 2005-10-05
US20030207300A1 (en) 2003-11-06
AU2003296989A8 (en) 2004-08-10
EP1581796A4 (en) 2008-02-13
WO2004063700A2 (en) 2004-07-29
JP2006518587A (en) 2006-08-17

Similar Documents

Publication Publication Date Title
WO2004063700A3 (en) Multiplex analytical platform using molecular tags
WO2006078289A3 (en) Bio-barcode based detection of target analytes
WO2005094421A3 (en) Methods for concurrent identification and quantification of an unknown bioagent
WO2008105896A3 (en) Analyte determination utilizing mass tagging reagents comprising a non-encoded detectable label
WO2005010494A3 (en) Multiplexed analyte detection
EP1978366A4 (en) Fluorescent label for biomaterial and method of fluorescent-labeling biomaterial
WO2002080647A3 (en) Methods and reagents for multiplexed analyte capture, surface array self-assembly, and analysis of complex biological samples
WO2010059731A3 (en) Polynucleotide mapping and sequencing
WO2006007567A3 (en) Methods and compositions to detect nucleic acids in a biological sample
WO2004099434A3 (en) Homogeneous multiplex screening assays and kits
WO2007050777A3 (en) Methods and compositions for diagnosing lung cancer with specific dna methylation patterns
WO2009022125A8 (en) Identification of nucleic acid sequences
WO2005106036A3 (en) Methods and compositions for assaying analytes
RU2007132732A (en) METHOD FOR QUANTITATIVE ANALYSIS OF MICROORGANISM WITH APPLICATION OF pPHK AS A TARGET
WO2007106047A8 (en) Nucleic acid interaction analysis
WO2007024840A3 (en) Method of quantitating nucleic acids by flow cytometry microparticle-based array
WO2005003394A3 (en) Bio-barcode based detection of target analytes
WO2001083823A8 (en) Methods and compositions for polynucleotide analysis using generic capture sequences
WO2006117161A3 (en) New labelling strategies for the sensitive detection of analytes
WO2008028086A3 (en) Methods and substances for isolation and detection of small polynucleotides
WO2006135437A3 (en) Real-time pcr microarray based on an evanescent wave biosensor
WO2002054084A8 (en) A method for diagnosing atrophic gastritis
AU2003298412A1 (en) Analytical platform and identification method
WO2007106802A3 (en) Method for linear amplification of bisulfite converted dna
WO2005071401A3 (en) Homogeneous multiplex assay for nucleic acid targets

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SK SL TJ TM TN TR TT TZ UA UG UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2004566540

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2003815205

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003815205

Country of ref document: EP