WO2004061119A3 - Rolling circle amplification of rna - Google Patents

Rolling circle amplification of rna Download PDF

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Publication number
WO2004061119A3
WO2004061119A3 PCT/US2003/039430 US0339430W WO2004061119A3 WO 2004061119 A3 WO2004061119 A3 WO 2004061119A3 US 0339430 W US0339430 W US 0339430W WO 2004061119 A3 WO2004061119 A3 WO 2004061119A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
rna molecules
sequence
strand cdna
rna
Prior art date
Application number
PCT/US2003/039430
Other languages
French (fr)
Other versions
WO2004061119A2 (en
Inventor
Gyanendra Kumar
Patricio Abarzua
Original Assignee
Molecular Staging Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Staging Inc filed Critical Molecular Staging Inc
Priority to AU2003297891A priority Critical patent/AU2003297891A1/en
Priority to JP2004565385A priority patent/JP2006512081A/en
Priority to AT03796961T priority patent/ATE469983T1/en
Priority to CA002512196A priority patent/CA2512196A1/en
Priority to EP03796961A priority patent/EP1585833B1/en
Priority to DE60332873T priority patent/DE60332873D1/en
Publication of WO2004061119A2 publication Critical patent/WO2004061119A2/en
Publication of WO2004061119A3 publication Critical patent/WO2004061119A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Abstract

Disclosed are compositions and methods for amplification of RNA molecules. The disclosed method involves synthesizing first strand cDNA molecules from RNA molecules, circularizing the first strand cDNA molecules and replicating the circularized first strand cDNA molecules using rolling circle replication. The method can be aided by the use of specialized primers for cDNA synthesis and specialized probes for circularizing the first strand cDNA molecules. The method can be used to replicate and amplify multiple RNA molecules, such as all RNA molecules in a sample or all mRNA molecules in a sample, or be used to replicate and amplify specific RNA molecules. Rolling circle replication of the circularized first strand cDNA molecules results in long DNA strands containing tandem repeats of the cDNA sequence. The tandem sequence DNA can be used directly (for detection of sequences, for example), further amplified, or used any other purpose. Doublestranded tandem sequence DNA can be used to produce unit lengths of the cDNA sequence. Tandem sequence DNA can also be transcribed to produce transcripts having sequence complementary to or matching the sequence of RNA molecules.
PCT/US2003/039430 2002-12-31 2003-12-11 Rolling circle amplification of rna WO2004061119A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2003297891A AU2003297891A1 (en) 2002-12-31 2003-12-11 Rolling circle amplification of rna
JP2004565385A JP2006512081A (en) 2002-12-31 2003-12-11 RNA rolling circle amplification
AT03796961T ATE469983T1 (en) 2002-12-31 2003-12-11 ROLLING-CIRCLE RNA AMPLIFICATION
CA002512196A CA2512196A1 (en) 2002-12-31 2003-12-11 Rolling circle amplification of rna
EP03796961A EP1585833B1 (en) 2002-12-31 2003-12-11 Rolling circle amplification of rna
DE60332873T DE60332873D1 (en) 2002-12-31 2003-12-11 ROLLING-CIRCLE RNA AMPLIFICATION

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/335,573 2002-12-31
US10/335,573 US6977153B2 (en) 2002-12-31 2002-12-31 Rolling circle amplification of RNA

Publications (2)

Publication Number Publication Date
WO2004061119A2 WO2004061119A2 (en) 2004-07-22
WO2004061119A3 true WO2004061119A3 (en) 2004-11-11

Family

ID=32655391

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/039430 WO2004061119A2 (en) 2002-12-31 2003-12-11 Rolling circle amplification of rna

Country Status (8)

Country Link
US (2) US6977153B2 (en)
EP (1) EP1585833B1 (en)
JP (1) JP2006512081A (en)
AT (1) ATE469983T1 (en)
AU (1) AU2003297891A1 (en)
CA (1) CA2512196A1 (en)
DE (1) DE60332873D1 (en)
WO (1) WO2004061119A2 (en)

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