WO2004044177A3 - Expression profiling based on histocultures - Google Patents

Expression profiling based on histocultures Download PDF

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Publication number
WO2004044177A3
WO2004044177A3 PCT/US2003/036238 US0336238W WO2004044177A3 WO 2004044177 A3 WO2004044177 A3 WO 2004044177A3 US 0336238 W US0336238 W US 0336238W WO 2004044177 A3 WO2004044177 A3 WO 2004044177A3
Authority
WO
WIPO (PCT)
Prior art keywords
histocultures
expression profiling
profiling based
rna
methods
Prior art date
Application number
PCT/US2003/036238
Other languages
French (fr)
Other versions
WO2004044177A2 (en
Inventor
Ping Jiang
Mingxu Xu
Yuying Tan
Original Assignee
Anticancer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anticancer Inc filed Critical Anticancer Inc
Priority to JP2004552178A priority Critical patent/JP4336654B2/en
Priority to KR20057008544A priority patent/KR101142716B1/en
Priority to EP20030789749 priority patent/EP1567672B1/en
Priority to CA 2505895 priority patent/CA2505895C/en
Priority to AT03789749T priority patent/ATE429514T1/en
Priority to AU2003294267A priority patent/AU2003294267B2/en
Priority to DE60327359T priority patent/DE60327359D1/en
Publication of WO2004044177A2 publication Critical patent/WO2004044177A2/en
Publication of WO2004044177A3 publication Critical patent/WO2004044177A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Abstract

Methods of obtaining faithful expression libraries from tissue samples comprise extraction of RNA from intact tissue cultured in three-dimensional sponge-gel based histocultures.
PCT/US2003/036238 2002-11-12 2003-11-12 Expression profiling based on histocultures WO2004044177A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2004552178A JP4336654B2 (en) 2002-11-12 2003-11-12 Expression profiling based on tissue culture
KR20057008544A KR101142716B1 (en) 2002-11-12 2003-11-12 Expression profiling based on histocultures
EP20030789749 EP1567672B1 (en) 2002-11-12 2003-11-12 Expression profiling based on histocultures
CA 2505895 CA2505895C (en) 2002-11-12 2003-11-12 Expression profiling based on histocultures
AT03789749T ATE429514T1 (en) 2002-11-12 2003-11-12 CREATION OF EXPRESSION PROFILES BASED ON HISTOCULTURES
AU2003294267A AU2003294267B2 (en) 2002-11-12 2003-11-12 Expression profiling based on histocultures
DE60327359T DE60327359D1 (en) 2002-11-12 2003-11-12 CREATING EXPRESSION PROFILES BASED ON HISTORIC CULTURES

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42594502P 2002-11-12 2002-11-12
US60/425,945 2002-11-12

Publications (2)

Publication Number Publication Date
WO2004044177A2 WO2004044177A2 (en) 2004-05-27
WO2004044177A3 true WO2004044177A3 (en) 2005-03-17

Family

ID=32313081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/036238 WO2004044177A2 (en) 2002-11-12 2003-11-12 Expression profiling based on histocultures

Country Status (10)

Country Link
US (2) US7252941B2 (en)
EP (1) EP1567672B1 (en)
JP (1) JP4336654B2 (en)
KR (1) KR101142716B1 (en)
CN (1) CN100572557C (en)
AT (1) ATE429514T1 (en)
AU (1) AU2003294267B2 (en)
CA (1) CA2505895C (en)
DE (1) DE60327359D1 (en)
WO (1) WO2004044177A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6203984B1 (en) * 1998-07-02 2001-03-20 Pacron, Inc. Proportional amplification of mRNA from a linear template in vitro

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5726009A (en) 1989-03-20 1998-03-10 Anticancer, Inc. Native-state method and system for determining viability and proliferative capacity of tissues in vitro
JP2950519B2 (en) 1991-02-28 1999-09-20 アンティキャンサー インコーポレイテッド Native tissue culture method for skin
US5474909A (en) * 1992-08-07 1995-12-12 Anticancer, Inc. Noncolorimetric histoculture method for predicting drug response of tumors
AU7357894A (en) * 1993-06-29 1995-01-24 Anticancer, Inc. Native-state method and system for determining viability and proliferative capacity of tissues (in vitro)

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6203984B1 (en) * 1998-07-02 2001-03-20 Pacron, Inc. Proportional amplification of mRNA from a linear template in vitro

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
SGROI ET AL.: "In Vivo Expression Profile Analysis Of Human Breast Cancer Progression", CANCER RESEARCH, vol. 59, November 1999 (1999-11-01), pages 5656 - 5661, XP002943416 *
SINGH ET AL.: "Prediction Of Survivial In Patients With Head and Neck Cancer Using The Histoculture Drug Response Assay", HEAD AND NECK, May 2002 (2002-05-01), pages 437 - 442, XP002982831 *
YATES ET AL.: "Early Shifts In Gene Expression During Chondroinduction Of Human Dermal Fibroblasts", EXPERIMENTAL CELL RESEARCH, vol. 265, 2001, pages 203 - 211, XP002966190 *

Also Published As

Publication number Publication date
US20040229234A1 (en) 2004-11-18
WO2004044177A2 (en) 2004-05-27
CA2505895C (en) 2013-10-22
AU2003294267A1 (en) 2004-06-03
AU2003294267B2 (en) 2009-01-08
EP1567672A2 (en) 2005-08-31
DE60327359D1 (en) 2009-06-04
ATE429514T1 (en) 2009-05-15
CA2505895A1 (en) 2004-05-27
US7252941B2 (en) 2007-08-07
JP2006506073A (en) 2006-02-23
EP1567672B1 (en) 2009-04-22
KR20050063805A (en) 2005-06-28
EP1567672A4 (en) 2006-08-16
KR101142716B1 (en) 2012-05-04
JP4336654B2 (en) 2009-09-30
US20080058222A1 (en) 2008-03-06
CN100572557C (en) 2009-12-23
CN1738910A (en) 2006-02-22

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