WO2004042347A3 - Compositions and methods for detection and isolation of phosphorylated molecules - Google Patents

Compositions and methods for detection and isolation of phosphorylated molecules Download PDF

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Publication number
WO2004042347A3
WO2004042347A3 PCT/US2003/013765 US0313765W WO2004042347A3 WO 2004042347 A3 WO2004042347 A3 WO 2004042347A3 US 0313765 W US0313765 W US 0313765W WO 2004042347 A3 WO2004042347 A3 WO 2004042347A3
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WO
WIPO (PCT)
Prior art keywords
binding
phosphate
phosphorylated
target molecules
isolation
Prior art date
Application number
PCT/US2003/013765
Other languages
French (fr)
Other versions
WO2004042347A2 (en
Inventor
Brian Agnew
Joseph Beechem
Kyle Gee
Richard Haugland
Jixiang Liu
Vladimir Martin
Wayne Patton
Thomas Steinberg
Original Assignee
Molecular Probes Inc
Brian Agnew
Joseph Beechem
Kyle Gee
Richard Haugland
Jixiang Liu
Vladimir Martin
Wayne Patton
Thomas Steinberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Probes Inc, Brian Agnew, Joseph Beechem, Kyle Gee, Richard Haugland, Jixiang Liu, Vladimir Martin, Wayne Patton, Thomas Steinberg filed Critical Molecular Probes Inc
Priority to CA002483868A priority Critical patent/CA2483868A1/en
Priority to AU2003299466A priority patent/AU2003299466A1/en
Priority to JP2004549877A priority patent/JP2005539243A/en
Priority to EP03799756A priority patent/EP1546118A4/en
Publication of WO2004042347A2 publication Critical patent/WO2004042347A2/en
Publication of WO2004042347A3 publication Critical patent/WO2004042347A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4709—Amyloid plaque core protein

Abstract

The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
PCT/US2003/013765 2002-05-03 2003-05-02 Compositions and methods for detection and isolation of phosphorylated molecules WO2004042347A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002483868A CA2483868A1 (en) 2002-05-03 2003-05-02 Compositions and methods for detection and isolation of phosphorylated molecules
AU2003299466A AU2003299466A1 (en) 2002-05-03 2003-05-02 Compositions and methods for detection and isolation of phosphorylated molecules
JP2004549877A JP2005539243A (en) 2002-05-03 2003-05-02 Compositions and methods for detection and separation of phosphorylated molecules
EP03799756A EP1546118A4 (en) 2002-05-03 2003-05-02 Compositions and methods for detection and isolation of phosphorylated molecules

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US37773302P 2002-05-03 2002-05-03
US60/377,733 2002-05-03
US39305902P 2002-06-28 2002-06-28
US60/393,059 2002-06-28
US40725502P 2002-08-30 2002-08-30
US60/407,255 2002-08-30
US44025203P 2003-01-14 2003-01-14
US60/440,252 2003-01-14

Publications (2)

Publication Number Publication Date
WO2004042347A2 WO2004042347A2 (en) 2004-05-21
WO2004042347A3 true WO2004042347A3 (en) 2005-04-14

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/013765 WO2004042347A2 (en) 2002-05-03 2003-05-02 Compositions and methods for detection and isolation of phosphorylated molecules

Country Status (7)

Country Link
US (1) US7102005B2 (en)
EP (1) EP1546118A4 (en)
JP (1) JP2005539243A (en)
CN (1) CN1665790A (en)
AU (1) AU2003299466A1 (en)
CA (1) CA2483868A1 (en)
WO (1) WO2004042347A2 (en)

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