WO2004009540A8 - Methods and kits for transferases - Google Patents

Methods and kits for transferases

Info

Publication number
WO2004009540A8
WO2004009540A8 PCT/US2003/022315 US0322315W WO2004009540A8 WO 2004009540 A8 WO2004009540 A8 WO 2004009540A8 US 0322315 W US0322315 W US 0322315W WO 2004009540 A8 WO2004009540 A8 WO 2004009540A8
Authority
WO
WIPO (PCT)
Prior art keywords
substrate
detected
activity
kits
transferase
Prior art date
Application number
PCT/US2003/022315
Other languages
French (fr)
Other versions
WO2004009540A2 (en
WO2004009540A3 (en
Inventor
Said A Goueli
Robert F Bulleit
Original Assignee
Promega Corp
Said A Goueli
Robert F Bulleit
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp, Said A Goueli, Robert F Bulleit filed Critical Promega Corp
Priority to JP2004523486A priority Critical patent/JP4402592B2/en
Priority to CA002492178A priority patent/CA2492178A1/en
Priority to AU2003253962A priority patent/AU2003253962B2/en
Priority to EP03765642A priority patent/EP1534853A4/en
Publication of WO2004009540A2 publication Critical patent/WO2004009540A2/en
Publication of WO2004009540A3 publication Critical patent/WO2004009540A3/en
Publication of WO2004009540A8 publication Critical patent/WO2004009540A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Abstract

A method for detecting transferase activity of a sample includes contacting the sample with a substrate and at least one of a phosphate group donor and a phosphate group acceptor. The substrate includes a reporter compound and amino acids. A peptidase is added that cleaves a non-phosphorylated substrate at a first rate and a phosphorylated substrate and a second rate. The output of the reporter compound is detected. In a preferred embodiment, the transferase activity detected is a kinase activity. In another preferred embodiment, the transferase activity detected is a phosphatase activity. Also provided is a method of screening for alterations in a transferase reaction. Kits and peptide substrate are also provided for carrying out at least one of the methods of the invention.
PCT/US2003/022315 2002-07-19 2003-07-17 Methods and kits for transferases WO2004009540A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2004523486A JP4402592B2 (en) 2002-07-19 2003-07-17 Methods and kits for transferase
CA002492178A CA2492178A1 (en) 2002-07-19 2003-07-17 Exopeptidase-mediated unquenching of reporter substrates in assays for kinase and phosphatase activity
AU2003253962A AU2003253962B2 (en) 2002-07-19 2003-07-17 Methods and kits for transferases
EP03765642A EP1534853A4 (en) 2002-07-19 2003-07-17 Methods and kits for transferases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/199,970 US7195884B2 (en) 2002-07-19 2002-07-19 Methods and kits for transferases
US10/199,970 2002-07-19

Publications (3)

Publication Number Publication Date
WO2004009540A2 WO2004009540A2 (en) 2004-01-29
WO2004009540A3 WO2004009540A3 (en) 2004-11-04
WO2004009540A8 true WO2004009540A8 (en) 2005-09-01

Family

ID=30443456

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/022315 WO2004009540A2 (en) 2002-07-19 2003-07-17 Methods and kits for transferases

Country Status (6)

Country Link
US (5) US7195884B2 (en)
EP (1) EP1534853A4 (en)
JP (2) JP4402592B2 (en)
AU (1) AU2003253962B2 (en)
CA (1) CA2492178A1 (en)
WO (1) WO2004009540A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1413887A1 (en) * 2002-10-22 2004-04-28 Aventis Pharma S.A. Inhibitors of Src kinase for use in Alzheimer's disease
CA2761125A1 (en) * 2009-05-21 2010-11-25 Schering Corporation Genetic markers associated with interferon-alpha response
ES2926463T3 (en) 2013-03-15 2022-10-26 Promega Corp Activation of bioluminescence by structural complementation

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4557862A (en) * 1983-10-28 1985-12-10 University Patents, Inc. Rhodamine derivatives as fluorogenic substrates for proteinases
DE3781185T2 (en) * 1986-04-01 1993-03-04 Wako Pure Chem Ind Ltd PEPTIDE DERIVATIVES AND METHOD FOR MEASURING THE EFFECTIVENESS OF PHYSIOLOGICALLY ACTIVE COMPOUNDS USING THE SAME AS SUBSTRATES.
EP0537270B1 (en) * 1990-07-02 1998-09-09 The Regents Of The University Of California Detection of analytes using fluorescent energy transfer
AU3129493A (en) * 1991-11-12 1993-06-15 Promega Corporation Non-radioactive enzyme assay
US6348310B1 (en) * 1994-03-04 2002-02-19 Promega Corporation Quantitation of individual protein kinase activity
US5763198A (en) * 1994-07-22 1998-06-09 Sugen, Inc. Screening assays for compounds
JP4155415B2 (en) 1996-02-19 2008-09-24 旭化成ファーマ株式会社 Measuring method of biological sample
US5912137A (en) * 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US5759787A (en) * 1996-08-26 1998-06-02 Tularik Inc. Kinase assay
GB9619168D0 (en) 1996-09-13 1996-10-23 Univ Cambridge Tech Assay
US5854011A (en) * 1996-12-19 1998-12-29 Idexx Laboratories Incorporated Method and components for the detection of yeasts and/or molds in a sample
US5917012A (en) * 1997-04-03 1999-06-29 Wako Pure Chemical Industries, Ltd. Peptide derivatives
US6184210B1 (en) * 1997-10-10 2001-02-06 Cytovia, Inc. Dipeptide apoptosis inhibitors and the use thereof
US6342611B1 (en) * 1997-10-10 2002-01-29 Cytovia, Inc. Fluorogenic or fluorescent reporter molecules and their applications for whole-cell fluorescence screening assays for capsases and other enzymes and the use thereof
WO1999029894A1 (en) * 1997-12-05 1999-06-17 Pharmacia & Upjohn Company Fluorescence-based high throughput screening assays for protein kinases and phosphatases
AU755273B2 (en) * 1998-03-16 2002-12-05 Cytovia, Inc. Dipeptide caspase inhibitors and the use thereof
US5869275A (en) * 1998-07-20 1999-02-09 Huang; Eric Z. Affinity ultrafiltration assay for transferase activity
DE69920473T2 (en) * 1998-07-21 2006-02-23 Cytovia, Inc., San Diego NEW FLUORESCENCE DYES AND THEIR APPLICATION IN FLUORESCENCE DETECTION METHODS OF FULL CELLS FOR CASAS, PEPTIDASES, PROTEASES AND OTHER ENZYMES AND THEIR USE
SE9803958D0 (en) 1998-11-19 1998-11-19 Pharmacia & Upjohn Ab Method of screening
EP1177168B1 (en) * 1999-04-09 2007-05-30 Cytovia, Inc. Caspase inhibitors and the use thereof
US6410255B1 (en) * 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US6280965B1 (en) * 1999-05-26 2001-08-28 Nova Southeastern University Simple non-radioactive assay for estimating protein kinase C and protein phosphatase-1
JP2001019700A (en) 1999-07-07 2001-01-23 Mitsubishi Chemicals Corp Fluorescence-labeled peptide
JP2001211937A (en) * 1999-08-27 2001-08-07 Tetsuji Nakamura Hair brush
GB9923208D0 (en) 1999-10-01 1999-12-08 Cambridge Drug Discovery Ltd Assay
US6261794B1 (en) * 1999-10-14 2001-07-17 Saint Louis University Methods for identifying inhibitors of methionine aminopeptidases
US20020004214A1 (en) 1999-12-21 2002-01-10 Raul Goldschmidt Method to detect modulators of histidine kinases
EP1182263A1 (en) 2000-08-11 2002-02-27 Evotec OAI AG Process for detecting threonine/serine kinase activity

Also Published As

Publication number Publication date
US20070184509A1 (en) 2007-08-09
US7314729B2 (en) 2008-01-01
WO2004009540A2 (en) 2004-01-29
EP1534853A2 (en) 2005-06-01
CA2492178A1 (en) 2004-01-29
JP4402592B2 (en) 2010-01-20
AU2003253962A1 (en) 2004-02-09
US20080057527A1 (en) 2008-03-06
JP2009106294A (en) 2009-05-21
WO2004009540A3 (en) 2004-11-04
AU2003253962B2 (en) 2007-08-09
US20080113396A1 (en) 2008-05-15
US20040248229A1 (en) 2004-12-09
JP2006508043A (en) 2006-03-09
US20040014159A1 (en) 2004-01-22
US7195884B2 (en) 2007-03-27
EP1534853A4 (en) 2008-03-12

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