WO2004003139A3 - Modified shine-dalgarno sequences and methods of use thereof - Google Patents

Modified shine-dalgarno sequences and methods of use thereof Download PDF

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Publication number
WO2004003139A3
WO2004003139A3 PCT/US2003/019786 US0319786W WO2004003139A3 WO 2004003139 A3 WO2004003139 A3 WO 2004003139A3 US 0319786 W US0319786 W US 0319786W WO 2004003139 A3 WO2004003139 A3 WO 2004003139A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
sequences
vectors
dalgarno sequences
shine
Prior art date
Application number
PCT/US2003/019786
Other languages
French (fr)
Other versions
WO2004003139A2 (en
Inventor
Michael W Laird
Original Assignee
Human Genome Sciences Inc
Michael W Laird
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Human Genome Sciences Inc, Michael W Laird filed Critical Human Genome Sciences Inc
Priority to EP03761989A priority Critical patent/EP1530634A4/en
Priority to AU2003256289A priority patent/AU2003256289A1/en
Publication of WO2004003139A2 publication Critical patent/WO2004003139A2/en
Publication of WO2004003139A3 publication Critical patent/WO2004003139A3/en
Priority to US11/004,853 priority patent/US7094573B2/en
Priority to US11/447,892 priority patent/US7405059B2/en
Priority to US12/142,313 priority patent/US20080274503A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Abstract

Novel Shine-Dalgarno (ribosome binding site) sequences, vectors containing such sequences, and host cells transformed with these vectors are provided. Methods of use of such sequences, vectors, and host cells for the efficient production of proteins and fragments thereof in prokaryotic systems are also provided. In particular embodiments of the invention, compounds and methods for high efficiency production of soluble protein in prokaryotic systems are provided.
PCT/US2003/019786 2002-06-26 2003-06-25 Modified shine-dalgarno sequences and methods of use thereof WO2004003139A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP03761989A EP1530634A4 (en) 2002-06-26 2003-06-25 Modified shine-dalgarno sequences and methods of use thereof
AU2003256289A AU2003256289A1 (en) 2002-06-26 2003-06-25 Modified shine-dalgarno sequences and methods of use thereof
US11/004,853 US7094573B2 (en) 2002-06-26 2004-12-07 Modified shine-dalgarno sequences and methods of use thereof
US11/447,892 US7405059B2 (en) 2002-06-26 2006-06-07 Modified Shine-Dalgarno sequences and methods of uses thereof
US12/142,313 US20080274503A1 (en) 2002-06-26 2008-06-19 Modified Shine-Dalgarno Sequences and Methods of Use Thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US39143302P 2002-06-26 2002-06-26
US60/391,433 2002-06-26
US40663002P 2002-08-29 2002-08-29
US60/406,630 2002-08-29

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/004,853 Continuation US7094573B2 (en) 2002-06-26 2004-12-07 Modified shine-dalgarno sequences and methods of use thereof

Publications (2)

Publication Number Publication Date
WO2004003139A2 WO2004003139A2 (en) 2004-01-08
WO2004003139A3 true WO2004003139A3 (en) 2004-04-08

Family

ID=30003191

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/019786 WO2004003139A2 (en) 2002-06-26 2003-06-25 Modified shine-dalgarno sequences and methods of use thereof

Country Status (4)

Country Link
US (3) US7094573B2 (en)
EP (1) EP1530634A4 (en)
AU (1) AU2003256289A1 (en)
WO (1) WO2004003139A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7601351B1 (en) 2002-06-26 2009-10-13 Human Genome Sciences, Inc. Antibodies against protective antigen
US7355027B2 (en) * 2004-06-16 2008-04-08 Dynport Vaccine Company Llc Bacillus anthracis protective antigen
US8101735B2 (en) 2004-06-16 2012-01-24 Health Protection Agency Preparation of protective antigen
WO2014047625A1 (en) * 2012-09-24 2014-03-27 Montana State University Recombinant lactococcus lactis expressing escherichia coli colonization factor antigen i (cfa/i) fimbriae and their methods of use
US20220119810A1 (en) * 2020-10-15 2022-04-21 Wisconsin Alumni Research Foundation High-efficacy crispri system and strong synthetic promoters for alphaproteobacteria and gammaproteobacteria

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6096545A (en) * 1996-07-31 2000-08-01 Queen's University At Kingston Phosphate starvation-inducible proteins
WO2000056883A1 (en) * 1999-03-23 2000-09-28 Human Genome Sciences, Inc. 49 human secreted proteins
WO2001082788A2 (en) * 2000-05-04 2001-11-08 President And Fellows Of Harvard College Compounds and methods for the treatment and prevention of bacterial infection

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3979506A (en) 1974-10-11 1976-09-07 Pierce Chemical Company Radioactive compounds for labeling proteins
US4358595A (en) 1981-10-13 1982-11-09 Pierce Chemical Company 4-Chloro-7-sulphobenzofurazan and salts thereof; new fluorigenic thiol specific reagents
US4582789A (en) 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
US5317098A (en) 1986-03-17 1994-05-31 Hiroaki Shizuya Non-radioisotope tagging of fragments
US5109124A (en) 1988-06-01 1992-04-28 Biogen, Inc. Nucleic acid probe linked to a label having a terminal cysteine
US5491224A (en) 1990-09-20 1996-02-13 Bittner; Michael L. Direct label transaminated DNA probe compositions for chromosome identification and methods for their manufacture
US6592872B1 (en) 1996-09-17 2003-07-15 The United States Of America As Represented By The Department Of Health And Human Services Targeting antigens to the MHC class I processing pathway with an anthrax toxin fusion protein
US6194168B1 (en) 1997-09-30 2001-02-27 Human Genome Sciences, Inc. Expression control sequences
US6069545A (en) * 1999-01-08 2000-05-30 Duraswitch Industries, Inc. Magnetically actuated pushbutton switch
CA2398207A1 (en) 1999-12-22 2001-06-28 The Ohio State University Research Foundation Methods for protecting against lethal infection with bacillus anthracis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6096545A (en) * 1996-07-31 2000-08-01 Queen's University At Kingston Phosphate starvation-inducible proteins
WO2000056883A1 (en) * 1999-03-23 2000-09-28 Human Genome Sciences, Inc. 49 human secreted proteins
WO2001082788A2 (en) * 2000-05-04 2001-11-08 President And Fellows Of Harvard College Compounds and methods for the treatment and prevention of bacterial infection

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1530634A4 *

Also Published As

Publication number Publication date
US20060216787A1 (en) 2006-09-28
EP1530634A4 (en) 2005-11-02
AU2003256289A8 (en) 2004-01-19
US7094573B2 (en) 2006-08-22
US20050153339A1 (en) 2005-07-14
US20080274503A1 (en) 2008-11-06
AU2003256289A1 (en) 2004-01-19
EP1530634A2 (en) 2005-05-18
US7405059B2 (en) 2008-07-29
WO2004003139A2 (en) 2004-01-08

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