WO2004002627A8 - Method and apparatus for fluid dispersion - Google Patents

Method and apparatus for fluid dispersion

Info

Publication number
WO2004002627A8
WO2004002627A8 PCT/US2003/020542 US0320542W WO2004002627A8 WO 2004002627 A8 WO2004002627 A8 WO 2004002627A8 US 0320542 W US0320542 W US 0320542W WO 2004002627 A8 WO2004002627 A8 WO 2004002627A8
Authority
WO
WIPO (PCT)
Prior art keywords
fluid dispersion
fluid
focusing
readily
available
Prior art date
Application number
PCT/US2003/020542
Other languages
French (fr)
Other versions
WO2004002627A2 (en
WO2004002627B1 (en
WO2004002627A3 (en
Inventor
Howard A Stone
Shelly L Anna
Nathalie Bontoux
Darren Roy Link
David A Weitz
Irina Gitlin
Piotr Garstecki
Willow Diluzio
George M Whitesides
Eugenia Kumacheva
Original Assignee
Harvard College
Howard A Stone
Shelly L Anna
Nathalie Bontoux
Darren Roy Link
David A Weitz
Irina Gitlin
Piotr Garstecki
Willow Diluzio
George M Whitesides
Eugenia Kumacheva
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2004549845A priority Critical patent/JP2006507921A/en
Application filed by Harvard College, Howard A Stone, Shelly L Anna, Nathalie Bontoux, Darren Roy Link, David A Weitz, Irina Gitlin, Piotr Garstecki, Willow Diluzio, George M Whitesides, Eugenia Kumacheva filed Critical Harvard College
Priority to AU2003253751A priority patent/AU2003253751B2/en
Priority to CN038204940A priority patent/CN1678397B/en
Priority to EP20030762228 priority patent/EP1515803A2/en
Priority to CA2491564A priority patent/CA2491564C/en
Publication of WO2004002627A2 publication Critical patent/WO2004002627A2/en
Publication of WO2004002627A3 publication Critical patent/WO2004002627A3/en
Publication of WO2004002627B1 publication Critical patent/WO2004002627B1/en
Priority to US11/024,228 priority patent/US7708949B2/en
Publication of WO2004002627A8 publication Critical patent/WO2004002627A8/en
Priority to US12/726,223 priority patent/US8337778B2/en
Priority to US13/679,190 priority patent/US8986628B2/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B01F23/41—Emulsifying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/45—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/45—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads
    • B01F25/452—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by elements provided with orifices or interstitial spaces
    • B01F25/4521—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by elements provided with orifices or interstitial spaces the components being pressed through orifices in elements, e.g. flat plates or cylinders, which obstruct the whole diameter of the tube
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
    • B01F33/3011—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
    • B05B7/02—Spray pistols; Apparatus for discharge
    • B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
    • B05B7/0408—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing two or more liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
    • B05B7/02—Spray pistols; Apparatus for discharge
    • B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
    • B05B7/0416—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
    • B05B7/02—Spray pistols; Apparatus for discharge
    • B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
    • B05B7/0416—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid
    • B05B7/0441—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid with one inner conduit of liquid surrounded by an external conduit of gas upstream the mixing chamber
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2215/00—Auxiliary or complementary information in relation with mixing
    • B01F2215/04—Technical information in relation with mixing
    • B01F2215/0413—Numerical information
    • B01F2215/0418—Geometrical information
    • B01F2215/0431—Numerical size values, e.g. diameter of a hole or conduit, area, volume, length, width, or ratios thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2215/00—Auxiliary or complementary information in relation with mixing
    • B01F2215/04—Technical information in relation with mixing
    • B01F2215/0413—Numerical information
    • B01F2215/0436—Operational information
    • B01F2215/045—Numerical flow-rate values
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S516/00—Colloid systems and wetting agents; subcombinations thereof; processes of
    • Y10S516/924—Significant dispersive or manipulative operation or step in making or stabilizing colloid system
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S516/00—Colloid systems and wetting agents; subcombinations thereof; processes of
    • Y10S516/924—Significant dispersive or manipulative operation or step in making or stabilizing colloid system
    • Y10S516/927—Significant dispersive or manipulative operation or step in making or stabilizing colloid system in situ formation of a colloid system making or stabilizing agent which chemical reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y10T137/0324—With control of flow by a condition or characteristic of a fluid
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y10T137/0324—With control of flow by a condition or characteristic of a fluid
    • Y10T137/0329—Mixing of plural fluids of diverse characteristics or conditions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/206—Flow affected by fluid contact, energy field or coanda effect [e.g., pure fluid device or system]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/8593—Systems
    • Y10T137/87265—Dividing into parallel flow paths with recombining
    • Y10T137/87338—Flow passage with bypass
    • Y10T137/87346—Including mixing feature
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T29/00—Metal working
    • Y10T29/49—Method of mechanical manufacture
    • Y10T29/49002—Electrical device making
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Abstract

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.
PCT/US2003/020542 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion WO2004002627A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP2004549845A JP2006507921A (en) 2002-06-28 2003-06-03 Method and apparatus for fluid dispersion
AU2003253751A AU2003253751B2 (en) 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion
CN038204940A CN1678397B (en) 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion
EP20030762228 EP1515803A2 (en) 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion
CA2491564A CA2491564C (en) 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion
US11/024,228 US7708949B2 (en) 2002-06-28 2004-12-28 Method and apparatus for fluid dispersion
US12/726,223 US8337778B2 (en) 2002-06-28 2010-03-17 Method and apparatus for fluid dispersion
US13/679,190 US8986628B2 (en) 2002-06-28 2012-11-16 Method and apparatus for fluid dispersion

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US39219502P 2002-06-28 2002-06-28
US60/392,195 2002-06-28
US42404202P 2002-11-05 2002-11-05
US60/424,042 2002-11-05

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/024,228 Continuation US7708949B2 (en) 2002-06-28 2004-12-28 Method and apparatus for fluid dispersion

Publications (4)

Publication Number Publication Date
WO2004002627A2 WO2004002627A2 (en) 2004-01-08
WO2004002627A3 WO2004002627A3 (en) 2004-04-01
WO2004002627B1 WO2004002627B1 (en) 2004-06-17
WO2004002627A8 true WO2004002627A8 (en) 2005-03-17

Family

ID=30003231

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/020542 WO2004002627A2 (en) 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion

Country Status (7)

Country Link
US (3) US7708949B2 (en)
EP (2) EP1515803A2 (en)
JP (2) JP2006507921A (en)
CN (2) CN102059162A (en)
AU (1) AU2003253751B2 (en)
CA (1) CA2491564C (en)
WO (1) WO2004002627A2 (en)

Families Citing this family (416)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
US7595195B2 (en) 2003-02-11 2009-09-29 The Regents Of The University Of California Microfluidic devices for controlled viscous shearing and formation of amphiphilic vesicles
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
WO2004091763A2 (en) 2003-04-10 2004-10-28 President And Fellows Of Harvard College Formation and control of fluidic species
KR20070029618A (en) 2003-08-27 2007-03-14 더 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Electronic control of fluidic species
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US8696952B2 (en) * 2004-04-23 2014-04-15 Eugenia Kumacheva Method of producing polymeric particles with selected size, shape, morphology and composition
NL1026261C2 (en) 2004-05-25 2005-11-28 Nanomi B V Spraying device with a nozzle plate provided with structures for promoting self-breakup, a nozzle plate, and methods for manufacturing and using such a nozzle plate.
GB0502398D0 (en) * 2005-02-04 2005-03-16 Q Chip Ltd Device and method for producing spherical segmented flow
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP1839760A1 (en) * 2005-01-17 2007-10-03 Universidad de Sevilla Method and device for the micromixing of fluids using a reflux cell
US20080213593A1 (en) * 2005-01-21 2008-09-04 President And Fellows Of Harvard College Systems And Methods For Forming Fluidic Droplets Encapsulated In Particles Such As Colloidal Particles
ES2390800T3 (en) 2005-01-28 2012-11-16 Duke University Apparatus and methods for handling droplets on a printed circuit board
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
CA2599683A1 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
JP2006289250A (en) * 2005-04-08 2006-10-26 Kao Corp Micro mixer and fluid mixing method using the same
US20060280029A1 (en) * 2005-06-13 2006-12-14 President And Fellows Of Harvard College Microfluidic mixer
US7955864B2 (en) * 2005-08-22 2011-06-07 Life Technologies Corporation Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
KR100928389B1 (en) * 2005-10-25 2009-11-23 메사추세츠 인스티튜트 오브 테크놀로지 Microstructure Synthesis by Flow Lithography and Polymerization
US7709544B2 (en) * 2005-10-25 2010-05-04 Massachusetts Institute Of Technology Microstructure synthesis by flow lithography and polymerization
GB2433448B (en) 2005-12-20 2011-03-02 Q Chip Ltd Method for the control of chemical processes
WO2007081387A1 (en) * 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices, methods of use, and kits for performing diagnostics
JP4713397B2 (en) 2006-01-18 2011-06-29 株式会社リコー Microchannel structure and microdroplet generation system
AU2007210152A1 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
EP2004328B1 (en) * 2006-03-09 2014-06-04 Agency for Science, Technology and Research Method for performing a reaction in a droplet
ES2776100T3 (en) * 2006-03-31 2020-07-29 Massachusetts Inst Technology System for targeted delivery of therapeutic agents
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8980198B2 (en) * 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
WO2007133590A2 (en) * 2006-05-08 2007-11-22 Auburn University Systems for and methods of characterizing reactions
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US9074242B2 (en) 2010-02-12 2015-07-07 Raindance Technologies, Inc. Digital analyte analysis
US20080003142A1 (en) * 2006-05-11 2008-01-03 Link Darren R Microfluidic devices
KR100848559B1 (en) * 2006-06-29 2008-07-25 엘지디스플레이 주식회사 FABRICATING METHOD OF SOFT MOLD AND pattern forming METHOD USING THEREOF
US7892434B2 (en) * 2006-08-02 2011-02-22 The Regents Of The University Of California Microfluidic production of monodispersed submicron emulsion through filtration and sorting of satellite drops
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
CA2665536C (en) 2006-10-05 2016-02-16 Massachusetts Institute Of Technology Multifunctional encoded particles for high-throughput analysis
US9874501B2 (en) 2006-11-24 2018-01-23 Curiox Biosystems Pte Ltd. Use of chemically patterned substrate for liquid handling, chemical and biological reactions
WO2008063135A1 (en) 2006-11-24 2008-05-29 Agency For Science, Technology And Research Apparatus for processing a sample in a liquid droplet and method of using the same
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
BRPI0806831B8 (en) 2007-02-09 2021-07-27 Advanced Liquid Logic Inc droplet actuating methods employing magnetic spheres
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
US7776927B2 (en) 2007-03-28 2010-08-17 President And Fellows Of Harvard College Emulsions and techniques for formation
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US8691164B2 (en) * 2007-04-20 2014-04-08 Celula, Inc. Cell sorting system and methods
WO2008148200A1 (en) * 2007-06-05 2008-12-11 Eugenia Kumacheva Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles
WO2009005680A1 (en) * 2007-06-29 2009-01-08 President And Fellows Of Harvard College Methods and apparatus for manipulation of fluidic species
GB0712863D0 (en) 2007-07-03 2007-08-08 Eastman Kodak Co Monodisperse droplet generation
GB0712861D0 (en) * 2007-07-03 2007-08-08 Eastman Kodak Co Continuous ink jet printing of encapsulated droplets
US20090068170A1 (en) * 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
WO2009020633A2 (en) * 2007-08-07 2009-02-12 President And Fellows Of Harvard College Metal oxide coating on surfaces
WO2009021215A1 (en) * 2007-08-09 2009-02-12 Celula, Inc. Methods and devices for correlated, multi-parameter single cell measurements and recovery of remnant biological material
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
JP5023902B2 (en) * 2007-09-06 2012-09-12 株式会社日立プラントテクノロジー Emulsifying device
US8685323B2 (en) * 2007-09-19 2014-04-01 Massachusetts Institute Of Technology Virus/nanowire encapsulation within polymer microgels for 2D and 3D devices for energy and electronics
US9744513B2 (en) 2007-09-20 2017-08-29 Jean-Louis Viovy Encapsulation microfluidic device
WO2009045050A2 (en) * 2007-10-05 2009-04-09 Seoul National University Industry Foundation Fluidic channel system and method for fabricating fine structure
US20090098168A1 (en) * 2007-10-08 2009-04-16 The Regents Of The University Of California Multiple-layer microbubble liposphere drug delivery vehicle and system
US10725020B2 (en) 2007-11-14 2020-07-28 Curiox Biosystems Pte Ltd. High throughput miniaturized assay system and methods
WO2013114217A1 (en) 2012-02-05 2013-08-08 Curiox Biosystems Pte Ltd. Array plates and methods for making and using same
JP5738597B2 (en) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ Systems and methods for nucleic acid sequencing
KR20100100974A (en) 2007-12-23 2010-09-15 어드밴스드 리퀴드 로직, 아이엔씨. Droplet actuator configurations and methods of conducting droplet operations
EP2271581A4 (en) * 2008-03-28 2014-09-03 Harvard College Surfaces, including microfluidic channels, with controlled wetting properties
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
CN102124259B (en) * 2008-05-16 2015-12-16 哈佛大学 Valve in the fluid system comprising microfluid system and other flowing control
JP4572973B2 (en) 2008-06-16 2010-11-04 ソニー株式会社 Microchip and flow-feeding method in microchip
EP2307560A2 (en) * 2008-06-27 2011-04-13 Massachusetts Institute of Technology Microfluidic droplets for metabolic engineering and other applications
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
JP2010038866A (en) * 2008-08-08 2010-02-18 Sony Corp Microchip, particulate dispensing device, and feed flow method
DE102008039117B3 (en) * 2008-08-21 2010-05-20 Institut für Bioprozess- und Analysenmesstechnik e.V. Arrangement and method for generating, manipulating and analyzing compartments
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
WO2011120024A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet generation for droplet-based assays
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
EP2373812B1 (en) 2008-12-19 2016-11-09 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
JP5909095B2 (en) * 2009-03-13 2016-04-26 プレジデント アンド フェローズ オブ ハーバード カレッジ Scale up microfluidic devices
WO2010104604A1 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Method for the controlled creation of emulsions, including multiple emulsions
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
WO2010111265A1 (en) 2009-03-24 2010-09-30 University Of Chicago Slip chip device and methods
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
WO2010110842A1 (en) 2009-03-25 2010-09-30 Eastman Kodak Company Droplet generator
US8689981B2 (en) 2009-04-10 2014-04-08 President And Fellows Of Harvard College Manipulation of particles in channels
EP4019977A1 (en) 2009-06-26 2022-06-29 President and Fellows of Harvard College Fluid injection
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
BR112012004719A2 (en) * 2009-09-02 2016-04-05 Harvard College multiple emulsions created by blasting and other techniques
JP6155418B2 (en) 2009-09-02 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド System for mixing fluids by combining multiple emulsions
US8746285B2 (en) * 2009-09-04 2014-06-10 Auburn University Programmable fluidic droplet generation
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
TWI421339B (en) * 2009-10-16 2014-01-01 Academia Sinica Method of fabricating three dimensional scaffolds and device thereof
US8513014B2 (en) 2009-10-20 2013-08-20 Academia Sinica Method for fabricating foam scaffolds to culture cells
CN102648053B (en) * 2009-10-27 2016-04-27 哈佛学院院长等 Drop formation technology
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
JP5823405B2 (en) 2009-11-04 2015-11-25 ザ ユニバーシティ オブ ブリティッシュ コロンビア Nucleic acid-containing lipid particles and related methods
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
WO2011066186A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US8399198B2 (en) 2010-03-02 2013-03-19 Bio-Rad Laboratories, Inc. Assays with droplets transformed into capsules
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
JP2013525087A (en) * 2010-03-17 2013-06-20 プレジデント アンド フェロウズ オブ ハーバード カレッジ Melt emulsification
EP2556170A4 (en) 2010-03-25 2014-01-01 Quantalife Inc Droplet transport system for detection
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
USD673286S1 (en) 2010-04-29 2012-12-25 Sony Corporation Micro flow channel chip
USD869308S1 (en) 2010-04-29 2019-12-10 Sony Corporation Micro flow channel chip
USD673287S1 (en) 2010-11-24 2012-12-25 Sony Corporation Micro flow channel chip
CA2802049C (en) 2010-06-07 2018-07-10 Firefly Bioworks, Inc. Scanning multifunctional particles
WO2012047324A2 (en) 2010-06-10 2012-04-12 President And Fellows Of Harvard College Systems and methods for amplification and phage display
US9878328B2 (en) 2010-07-23 2018-01-30 Curiox Biosystems Pte Ltd. Apparatus and method for multiple reactions in small volumes
JP2012024313A (en) * 2010-07-23 2012-02-09 Nitto Denko Corp Device for forming droplets, and method for forming droplets
EP2608878A4 (en) 2010-08-23 2017-11-15 President and Fellows of Harvard College Acoustic waves in microfluidics
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
EP3574990B1 (en) 2010-11-01 2022-04-06 Bio-Rad Laboratories, Inc. System for forming emulsions
JP6118725B2 (en) 2010-11-12 2017-04-19 ジェン９・インコーポレイテッドＧｅｎ９，ＩＮＣ． Methods and devices for nucleic acid synthesis
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
WO2012087350A2 (en) 2010-12-21 2012-06-28 President And Fellows Of Harvard College Spray drying techniques
JP2014505594A (en) 2011-02-07 2014-03-06 プレジデント アンド フェロウズ オブ ハーバード カレッジ System and method for splitting droplets
EP2673382B1 (en) 2011-02-11 2020-05-06 Bio-Rad Laboratories, Inc. Thermocycling device for nucleic acid amplification and methods of use
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
WO2012135327A1 (en) 2011-03-31 2012-10-04 Gnubio Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
WO2012135664A2 (en) 2011-03-31 2012-10-04 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex cold-pcr
CA2834291A1 (en) 2011-04-25 2012-11-01 Biorad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US9188615B2 (en) 2011-05-09 2015-11-17 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
JP6122843B2 (en) 2011-05-23 2017-04-26 プレジデント アンド フェローズ オブ ハーバード カレッジ Emulsion control including multiple emulsions
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
AU2012279420A1 (en) 2011-07-06 2014-01-30 Advanced Liquid Logic Inc Reagent storage on a droplet actuator
CN103764265A (en) 2011-07-06 2014-04-30 哈佛学院院长等 Multiple emulsions and techniques for the formation of multiple emulsions
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013016413A2 (en) 2011-07-25 2013-01-31 Advanced Liquid Logic Inc Droplet actuator apparatus and system
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
JP2014526899A (en) 2011-08-26 2014-10-09 ジェン９・インコーポレイテッド Compositions and methods for high fidelity assembly of nucleic acids
CN103764272A (en) 2011-08-30 2014-04-30 哈佛学院院长等 Systems and methods for shell encapsulation
SG11201401810QA (en) 2011-09-28 2014-10-30 Harvard College Systems and methods for droplet production and/or fluidic manipulation
EP3069785A1 (en) 2011-10-25 2016-09-21 The University Of British Columbia Limit size lipid nanoparticles and related methods
CN102500489A (en) * 2011-11-06 2012-06-20 中国科学技术大学 Spray gun spray nozzle capable of realizing minuteness atomization
WO2013078216A1 (en) 2011-11-21 2013-05-30 Advanced Liquid Logic Inc Glucose-6-phosphate dehydrogenase assays
US8771611B2 (en) 2011-11-28 2014-07-08 Auburn University System and methods of log-scale concentration gradients
EP2812103B1 (en) 2012-02-08 2016-08-03 President and Fellows of Harvard College Droplet formation using fluid breakup
EP3495817A1 (en) 2012-02-10 2019-06-12 Raindance Technologies, Inc. Molecular diagnostic screening assay
WO2013126741A1 (en) 2012-02-24 2013-08-29 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
WO2013134261A1 (en) 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
JP6115930B2 (en) * 2012-03-12 2017-04-19 国立研究開発法人産業技術総合研究所 Multi-stage split channel mixer
US10080997B2 (en) * 2012-03-16 2018-09-25 Versitech Limited System and method for generation of emulsions with low interfacial tension and measuring frequency vibrations in the system
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
WO2013141695A1 (en) * 2012-03-22 2013-09-26 Universiteit Twente Apparatus and method for mass producing a monodisperse microbubble agent
US8936353B2 (en) 2012-03-28 2015-01-20 Eastman Kodak Company Digital drop patterning device and method
US8936354B2 (en) 2012-03-28 2015-01-20 Eastman Kodak Company Digital drop patterning device and method
US8602535B2 (en) 2012-03-28 2013-12-10 Eastman Kodak Company Digital drop patterning device and method
US8939551B2 (en) 2012-03-28 2015-01-27 Eastman Kodak Company Digital drop patterning device and method
US20150177115A1 (en) 2012-04-06 2015-06-25 Slingshot Biosciences Hydrogel particles with tunable optical properties
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9808798B2 (en) 2012-04-20 2017-11-07 California Institute Of Technology Fluidic devices for biospecimen preservation
CN104428651B (en) 2012-04-20 2019-01-11 达丽斯生物医学公司 The fluid means and system for sample preparation or independently analyzed
US9803237B2 (en) 2012-04-24 2017-10-31 California Institute Of Technology Slip-induced compartmentalization
EP4001427A1 (en) 2012-04-24 2022-05-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2013163246A2 (en) 2012-04-25 2013-10-31 President And Fellows Of Harvard College Polymerization reactions within microfluidic devices
EP2844768B1 (en) 2012-04-30 2019-03-13 Raindance Technologies, Inc. Digital analyte analysis
AU2013280661A1 (en) 2012-06-25 2015-01-22 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
JP6222671B2 (en) 2012-06-27 2017-11-01 アドバンスト リキッド ロジック インコーポレイテッドＡｄｖａｎｃｅｄ Ｌｉｑｕｉｄ Ｌｏｇｉｃ， Ｉｎｃ． Technology and droplet actuator design to reduce bubble formation
CA2881783A1 (en) 2012-08-13 2014-02-20 The Regents Of The University Of California Methods and systems for detecting biological components
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
CN111748607A (en) 2012-08-14 2020-10-09 10X基因组学有限公司 Microcapsule compositions and methods
US9328376B2 (en) * 2012-09-05 2016-05-03 Bio-Rad Laboratories, Inc. Systems and methods for stabilizing droplets
CN108479872B (en) 2012-09-12 2021-02-12 基纽拜奥股份有限公司 Integrated microfluidic systems, methods and kits for performing assays
WO2014047236A2 (en) 2012-09-21 2014-03-27 President And Fellows Of Harvard College Systems and methods for spray drying in microfluidic and other systems
WO2014085801A1 (en) 2012-11-30 2014-06-05 The Broad Institute, Inc. Cryo-treatment in a microfluidic device
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2900543C (en) 2013-02-08 2023-01-31 10X Genomics, Inc. Partitioning and processing of analytes and other species
CN108212237B (en) * 2013-03-06 2020-12-08 哈佛学院院长及董事 Apparatus and method for forming relatively monodisperse droplets
WO2014153071A1 (en) 2013-03-14 2014-09-25 The Broad Institute, Inc. Methods for quantitating dna using digital multiple displacement amplification
WO2014172045A1 (en) 2013-03-15 2014-10-23 The University Of British Columbia Lipid nanoparticles for transfection and related methods
GB2525568B (en) 2013-03-15 2020-10-14 Abvitro Llc Single cell barcoding for antibody discovery
GB201306444D0 (en) 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
GB201306445D0 (en) 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
EP2986762B1 (en) 2013-04-19 2019-11-06 Bio-Rad Laboratories, Inc. Digital analyte analysis
CN103285946A (en) * 2013-05-27 2013-09-11 苏州扬清芯片科技有限公司 Biochip and control method thereof
CN103285947A (en) * 2013-05-27 2013-09-11 苏州扬清芯片科技有限公司 Droplet micro-fluidic chip and operation method thereof
KR20140144408A (en) * 2013-06-11 2014-12-19 삼성전기주식회사 Droplet forming device and method for forming droplet using the same
US11141730B2 (en) 2013-06-14 2021-10-12 President And Fellows Of Harvard College Coalescence of droplets
US9557318B2 (en) 2013-07-09 2017-01-31 Curiox Biosystems Pte Ltd. Array plates for washing samples
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9233859B2 (en) 2013-09-30 2016-01-12 Uchicago Argonne, Llc. Microfluidic process monitor for industrial solvent extraction system
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US20160279068A1 (en) 2013-11-08 2016-09-29 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
WO2015077717A1 (en) 2013-11-25 2015-05-28 The Broad Institute Inc. Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status
US11725237B2 (en) 2013-12-05 2023-08-15 The Broad Institute Inc. Polymorphic gene typing and somatic change detection using sequencing data
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US11452768B2 (en) 2013-12-20 2022-09-27 The Broad Institute, Inc. Combination therapy with neoantigen vaccine
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
EP3514246B1 (en) 2014-02-27 2021-11-17 The Broad Institute, Inc. T cell balance gene expression and methods of use thereof
EP3117897A4 (en) * 2014-03-11 2017-10-18 Toppan Printing Co., Ltd. Droplet producing device, droplet producing method, liposome producing method, fixture, and droplet producing kit
MX2016013156A (en) 2014-04-10 2017-02-14 10X Genomics Inc Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same.
WO2015160919A1 (en) 2014-04-16 2015-10-22 President And Fellows Of Harvard College Systems and methods for producing droplet emulsions with relatively thin shells
AU2015250034B2 (en) 2014-04-21 2019-06-20 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
WO2015173651A1 (en) 2014-05-14 2015-11-19 Mark Davies Microfluidic device with channel plates
WO2015195698A1 (en) 2014-06-16 2015-12-23 Gnubio, Inc. Size alternating injection into drops to facilitate sorting
SG11201610691QA (en) 2014-06-26 2017-01-27 10X Genomics Inc Processes and systems for nucleic acid sequence assembly
KR102531677B1 (en) 2014-06-26 2023-05-10 10엑스 제노믹스, 인크. Methods of analyzing nucleic acids from individual cells or cell populations
WO2015200616A1 (en) 2014-06-26 2015-12-30 President And Fellows Of Harvard College Fluid injection using acoustic waves
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
AR101397A1 (en) 2014-08-06 2016-12-14 Johnson & Son Inc S C INSERT FOR SPRAYERS
JP2017532024A (en) 2014-09-09 2017-11-02 ザ・ブロード・インスティテュート・インコーポレイテッド Droplet-based methods and instruments for composite single cell nucleic acid analysis
EP3536786B1 (en) 2014-09-15 2021-06-30 AbVitro LLC High-throughput nucleotide library sequencing
CA3001986C (en) 2014-10-22 2023-02-21 The Regents Of The University Of California High definition microdroplet printer
EP3212807B1 (en) 2014-10-29 2020-09-02 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
JP6742311B2 (en) 2014-11-14 2020-08-19 アテナ ダイアグナスティクス，インコーポレイテッド Method for detecting silent carrier genotype
WO2016085743A1 (en) 2014-11-24 2016-06-02 President And Fellows Of Harvard College Methods and systems for encapsulation of actives within droplets and other compartments
US10993997B2 (en) 2014-12-19 2021-05-04 The Broad Institute, Inc. Methods for profiling the t cell repertoire
WO2016100975A1 (en) 2014-12-19 2016-06-23 Massachsetts Institute Ot Technology Molecular biomarkers for cancer immunotherapy
MX367432B (en) 2015-01-12 2019-08-08 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same.
EP4092681A1 (en) 2015-01-13 2022-11-23 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
NL2014178B1 (en) * 2015-01-22 2017-01-05 Tide Microfluidics B V System and method for controlled manufacturing of mono-disperse microbubbles.
EP3247675A4 (en) 2015-01-23 2018-07-04 Neofluidics LLC A microfluidic serial dilution platform based well-plate using an oil-free immiscible phase driven by manual or electronic pipettors
US20160238506A1 (en) * 2015-02-02 2016-08-18 Derek Oberreit Ice nucleii counter technology
JP2018508198A (en) 2015-02-04 2018-03-29 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Nucleic acid sequencing by barcode addition in separate entities
US20160358722A1 (en) * 2015-02-05 2016-12-08 Ramasamy Lakshmanan Intelligent wireless and wired control of devices
CN107208156B (en) 2015-02-09 2021-10-08 10X基因组学有限公司 System and method for determining structural variation and phasing using variation recognition data
KR102564360B1 (en) 2015-02-09 2023-08-04 슬링샷 바이오사이언시즈 인코포레이티드 Hydrogel particles with tunable optical properties and methods for using the same
KR20170119710A (en) 2015-02-24 2017-10-27 10엑스 제노믹스, 인크. Targeted nucleic acid sequence coverage method
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
WO2016138488A2 (en) 2015-02-26 2016-09-01 The Broad Institute Inc. T cell balance gene expression, compositions of matters and methods of use thereof
WO2016145409A1 (en) 2015-03-11 2016-09-15 The Broad Institute, Inc. Genotype and phenotype coupling
US10876156B2 (en) 2015-03-13 2020-12-29 President And Fellows Of Harvard College Determination of cells using amplification
US11746367B2 (en) 2015-04-17 2023-09-05 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
JP6913032B2 (en) 2015-05-20 2021-08-04 ザ・ブロード・インスティテュート・インコーポレイテッド Common neoantigen
CN107405633A (en) * 2015-05-22 2017-11-28 香港科技大学 Droplet generator based on high-aspect-ratio inductive formation drop
US10545139B2 (en) 2015-06-16 2020-01-28 Curiox Biosystems Pte Ltd. Methods and devices for performing biological assays using magnetic components
WO2016205728A1 (en) 2015-06-17 2016-12-22 Massachusetts Institute Of Technology Crispr mediated recording of cellular events
WO2016207721A1 (en) 2015-06-25 2016-12-29 University Of Limerick Mechanical device for generating combinatorial library
WO2017015414A1 (en) 2015-07-23 2017-01-26 Zito Jr Arthur J Responsive dispersion from compartment in aqueous solution
WO2017034925A1 (en) 2015-08-25 2017-03-02 Bio-Rad Laboratories, Inc. Digital immunoassay
WO2017035287A1 (en) 2015-08-27 2017-03-02 President And Fellows Of Harvard College Acoustic wave sorting
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
CA2999888A1 (en) 2015-09-24 2017-03-30 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
US20180274021A1 (en) 2015-09-24 2018-09-27 Abvitro Llc Single amplicon activated exclusion pcr
EP3353550A1 (en) 2015-09-25 2018-08-01 AbVitro LLC High throughput process for t cell receptor target identification of natively-paired t cell receptor sequences
EP3362032A4 (en) 2015-10-13 2019-05-01 President and Fellows of Harvard College Systems and methods for making and using gel microspheres
EP3862088A1 (en) 2015-10-27 2021-08-11 Berkeley Lights, Inc. Method of manufcturing microfluidic electrowetting device having a covalently bound hydrophobic surface
US11092607B2 (en) 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
WO2017075297A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. High-throughput dynamic reagent delivery system
CN105435869B (en) * 2015-11-06 2017-05-10 常州工学院 Apparatus and method for micro-droplet split in microchannel
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
WO2017087554A1 (en) 2015-11-20 2017-05-26 Gnubio, Inc. Sparse identity spaces in droplet sequencing
PT3882357T (en) 2015-12-04 2022-09-05 10X Genomics Inc Methods and compositions for nucleic acid analysis
EP3263715B1 (en) 2016-06-28 2020-01-08 Hifibio Method for transcriptome analysis of single cells
WO2017124101A2 (en) 2016-01-15 2017-07-20 The Broad Institute Inc. Semi-permeable arrays for analyzing biological systems and methods of using same
US10962527B2 (en) 2016-02-05 2021-03-30 The Broad Institute, Inc. Multi-stage, multiplexed target isolation and processing from heterogeneous populations
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
EP3420102B1 (en) 2016-02-22 2024-04-03 Massachusetts Institute of Technology Methods for identifying and modulating immune phenotypes
US11427861B2 (en) 2016-03-17 2022-08-30 Massachusetts Institute Of Technology Methods for identifying and modulating co-occurant cellular phenotypes
ES2952832T3 (en) 2016-03-28 2023-11-06 Ncan Genomics Inc Linked Duplex Target Capture
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
US11925933B2 (en) 2016-04-15 2024-03-12 President And Fellows Of Harvard College Systems and methods for the collection of droplets and/or other entities
CN105712319B (en) * 2016-04-29 2018-01-23 清华大学 The preparation facilities of macroscopical aggregation of micro Nano material
CA3022916C (en) 2016-05-03 2020-03-10 Pneuma Respiratory, Inc. Droplet delivery device for delivery of fluids to the pulmonary system and methods of use
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
EP3481540B1 (en) 2016-07-08 2022-12-21 President and Fellows of Harvard College Formation of colloids or gels within droplets
EP3481968A4 (en) 2016-07-08 2019-12-18 California Institute of Technology Methods and devices for performing flow-through capture of low-concentration analytes
CN110088290A (en) 2016-08-10 2019-08-02 加利福尼亚大学董事会 Multiple displacement amplification and PCR are combined in lotion droplet
US10654040B2 (en) 2016-08-18 2020-05-19 Northeastern University Platform for liquid droplet formation and isolation
AU2017332495A1 (en) 2016-09-24 2019-04-11 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
CA3048420A1 (en) 2016-12-09 2018-06-14 Boreal Genomics, Inc. Linked ligation
EP3571308A4 (en) 2016-12-21 2020-08-19 The Regents of The University of California Single cell genomic sequencing using hydrogel based droplets
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140391A1 (en) 2017-01-24 2018-08-02 The Broad Institute, Inc. Compositions and methods for detecting a mutant variant of a polynucleotide
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
LT3375889T (en) 2017-03-17 2020-06-25 Hifibio Sas Single cell analysis
US20200115753A1 (en) 2017-03-17 2020-04-16 Massachusetts Institute Of Technology Methods for identifying and modulating co-occurant cellular phenotypes
WO2018185554A1 (en) 2017-04-05 2018-10-11 Curiox Biosystems Pte Ltd. Methods, devices, and apparatus for washing samples on array plates
US20210293783A1 (en) 2017-04-18 2021-09-23 The General Hospital Corporation Compositions for detecting secretion and methods of use
EP3615220A4 (en) 2017-04-28 2020-12-30 Neofluidics, LLC Fluidic devices with reaction wells and uses thereof
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
CN110621786A (en) 2017-05-15 2019-12-27 贝斯4创新公司 Mononucleotide detecting method and relevant probe
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
WO2018213643A1 (en) 2017-05-18 2018-11-22 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP3634552A4 (en) 2017-05-19 2021-03-03 Pneuma Respiratory, Inc. Dry powder delivery device and methods of use
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
WO2018217831A1 (en) 2017-05-22 2018-11-29 Arizona Board Of Regents On Behalf Of Arizona State University Metal electrode based 3d printed device for tuning microfluidic droplet generation frequency and synchronizing phase for serial femtosecond crystallography
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
KR102550778B1 (en) 2017-05-26 2023-07-03 에이비비트로 엘엘씨 High-throughput polynucleotide library sequencing and transcriptome analysis
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2019032690A1 (en) 2017-08-09 2019-02-14 Neofluidics, Llc Devices and methods for bioassay
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
WO2019051335A1 (en) 2017-09-07 2019-03-14 Juno Therapeutics, Inc. Methods of identifying cellular attributes related to outcomes associated with cell therapy
JP2020536614A (en) 2017-10-04 2020-12-17 ニューマ・リスパイラトリー・インコーポレイテッド In-line droplet delivery device and usage that is electrically operated by breathing
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
CA3079189A1 (en) 2017-10-17 2019-04-25 Pneuma Respiratory, Inc. Nasal drug delivery apparatus and methods of use
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
WO2019079301A2 (en) 2017-10-18 2019-04-25 Group K Diagnostics, Inc. Single-layer microfluidic device and methods of manufacture and use thereof
US11732257B2 (en) 2017-10-23 2023-08-22 Massachusetts Institute Of Technology Single cell sequencing libraries of genomic transcript regions of interest in proximity to barcodes, and genotyping of said libraries
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Microfluidic channel networks for partitioning
WO2019084165A1 (en) 2017-10-27 2019-05-02 10X Genomics, Inc. Methods and systems for sample preparation and analysis
CN111479604B (en) 2017-11-08 2022-12-30 精呼吸股份有限公司 Electrically breath actuated in-line droplet delivery device with small volume ampoule and method of use
JP7256198B2 (en) 2017-11-10 2023-04-11 ネオフルーイディクス，リミティド ライアビリティ カンパニー Integrated fluidic circuits and devices for droplet manipulation and methods thereof
CN111051523B (en) 2017-11-15 2024-03-19 10X基因组学有限公司 Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN107941659B (en) * 2017-11-20 2020-05-19 武汉科技大学 Seepage velocity measuring device in fracture water freezing process
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
US11173487B2 (en) 2017-12-19 2021-11-16 Arizona Board Of Regents On Behalf Of Arizona State University Deterministic ratchet for sub-micrometer bioparticle separation
BR112020013122A2 (en) 2017-12-28 2020-12-01 Ethicon Llc surgical instrument comprising a control system that uses signals from a strain gauge circuit
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
WO2019241488A1 (en) * 2018-06-14 2019-12-19 Regents Of The University Of Minnesota Counterflow mixer and atomizer
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN112996605A (en) * 2018-10-01 2021-06-18 精呼吸股份有限公司 Delivery of low surface tension compositions to the pulmonary system via an electronic breath-actuated droplet delivery device
US20220411783A1 (en) 2018-10-12 2022-12-29 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
GB201817321D0 (en) 2018-10-24 2018-12-05 Nanna Therapeutics Ltd Microbeads for tagless encoded chemical library screening
USD879999S1 (en) 2018-11-02 2020-03-31 Group K Diagnostics, Inc. Microfluidic device
SG11202105082SA (en) 2018-11-14 2021-06-29 Harvard College Multiplexing highly evolving viral variants with sherlock detection method
WO2020102610A1 (en) 2018-11-14 2020-05-22 The Broad Institute, Inc. Crispr system based droplet diagnostic systems and methods
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123657A2 (en) 2018-12-11 2020-06-18 10X Genomics, Inc. Methods and devices for detecting and sorting droplets or particles
WO2020124050A1 (en) 2018-12-13 2020-06-18 The Broad Institute, Inc. Tiled assays using crispr-cas based detection
WO2020131586A2 (en) 2018-12-17 2020-06-25 The Broad Institute, Inc. Methods for identifying neoantigens
EP3670667A1 (en) 2018-12-19 2020-06-24 Paris Sciences et Lettres - Quartier Latin Identification of cognate pairs of ligands and receptors
WO2020139844A1 (en) 2018-12-24 2020-07-02 10X Genomics, Inc. Devices, systems, and methods for controlling liquid flow
US11473136B2 (en) 2019-01-03 2022-10-18 Ncan Genomics, Inc. Linked target capture
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US20220119871A1 (en) 2019-01-28 2022-04-21 The Broad Institute, Inc. In-situ spatial transcriptomics
EP3924505A1 (en) 2019-02-12 2021-12-22 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
EP3698871A1 (en) 2019-02-19 2020-08-26 Gottfried Wilhelm Leibniz Universität Hannover Laser based sorting of droplets in microfluidic streams
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020176449A1 (en) 2019-02-26 2020-09-03 President And Fellows Of Harvard College Systems and methods for high throughput selection
CN113747974A (en) 2019-02-28 2021-12-03 10X基因组学有限公司 Apparatus, system, and method for improving droplet formation efficiency
CN113767178A (en) 2019-03-11 2021-12-07 10X基因组学有限公司 Systems and methods for processing optically labeled beads
US11318487B2 (en) * 2019-05-14 2022-05-03 Arizona Board Of Regents On Behalf Of Arizona State University Co-flow injection for serial crystallography
US11624718B2 (en) 2019-05-14 2023-04-11 Arizona Board Of Regents On Behalf Of Arizona State University Single piece droplet generation and injection device for serial crystallography
EP3990071A4 (en) * 2019-06-27 2023-07-19 Pneuma Respiratory, Inc. Delivery of small droplets to the respiratory system via electronic breath actuated droplet delivery device
EP3763439A1 (en) * 2019-07-12 2021-01-13 Curiosity Diagnostics sp. z o.o Microfluidic chip and valve, production process and uses
CN110404701A (en) * 2019-07-16 2019-11-05 清华大学 A kind of control method and device of jet crushing
US11701658B2 (en) 2019-08-09 2023-07-18 President And Fellows Of Harvard College Systems and methods for microfluidic particle selection, encapsulation, and injection using surface acoustic waves
US11919002B2 (en) 2019-08-20 2024-03-05 10X Genomics, Inc. Devices and methods for generating and recovering droplets
GB201914537D0 (en) 2019-10-08 2019-11-20 Univ Southampton Transcript analysis
CN114829626A (en) 2019-10-10 2022-07-29 1859公司 Methods and systems for microfluidic screening
CN110787846B (en) * 2019-11-05 2021-04-16 华中科技大学 One-step double-layer micro-droplet generation device and method
AU2021209907A1 (en) 2020-01-24 2022-07-07 Slingshot Biosciences, Inc. Compositions and methods for cell-like calibration particles
US20230158502A1 (en) 2020-04-17 2023-05-25 Sphere Fluidics Limited Droplet spacing
CN115485556A (en) 2020-05-04 2022-12-16 弹弓生物科学公司 Compositions and methods for passive optical barcoding for multiplexed assays
US11701668B1 (en) 2020-05-08 2023-07-18 10X Genomics, Inc. Methods and devices for magnetic separation
US11594340B2 (en) 2020-05-13 2023-02-28 Battelle Savannah River Alliance, Llc Manufacture of particulate reference materials
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US11946038B1 (en) 2020-05-29 2024-04-02 10X Genomics, Inc. Methods and systems including flow and magnetic modules
CN115997125A (en) 2020-06-24 2023-04-21 高诚生物医药(香港)有限公司 Method for identifying cognate pairs of ligand and receptor
EP3950772A1 (en) 2020-08-05 2022-02-09 Emulseo SAS Novel fluorosurfactants and uses thereof in microfluidics
WO2022051529A1 (en) 2020-09-02 2022-03-10 10X Genomics, Inc. Devices, systems, and methods for high throughput droplet formation
CN116367913A (en) 2020-09-02 2023-06-30 10X基因组学有限公司 Flow focusing apparatus, systems, and methods for high throughput drop formation
US11485632B2 (en) 2020-10-09 2022-11-01 Arizona Board Of Regents On Behalf Of Arizona State University Modular 3-D printed devices for sample delivery and method
WO2022146770A1 (en) 2020-12-28 2022-07-07 Neofluidics Llc A microfluidic serial dilution platform based well-plate using an oil-free immiscible phase driven by manual or electronic pipettors and method of operation
CN117098607A (en) 2021-02-24 2023-11-21 10X基因组学有限公司 Method for concentrating droplets in an emulsion
EP4313412A1 (en) 2021-03-26 2024-02-07 10X Genomics, Inc. Devices, methods, and systems for improved droplet recovery
EP4330421A1 (en) 2021-04-26 2024-03-06 The Brigham and Women's Hospital, Inc. Compositions and methods for characterizing polynucleotide sequence alterations
KR20240037245A (en) 2021-06-22 2024-03-21 뉴마 레스퍼러토리 인코포레이티드 Droplet delivery device by push ejection
WO2023004068A2 (en) 2021-07-21 2023-01-26 10X Genomics, Inc. Methods, devices, and kits for purifying and lysing biological particles
WO2023021295A2 (en) 2021-08-18 2023-02-23 Nuclera Nucleics Ltd Methods and compositions for improved biomolecule assays on digital microfluidic devices
WO2023076629A1 (en) 2021-10-29 2023-05-04 Slingshot Biosciences, Inc. Hydrogel particles as feeder cells and as synthetic antigen presenting cells
WO2023099667A1 (en) 2021-12-01 2023-06-08 Vilnius University Methods for processing and barcoding nucleic acids
WO2023168423A1 (en) 2022-03-04 2023-09-07 10X Genomics, Inc. Droplet forming devices and methods having fluoropolymer silane coating agents
CN114643088A (en) * 2022-03-14 2022-06-21 常熟理工学院 Micro-droplet generation chip based on Karman vortex street
WO2023215886A1 (en) 2022-05-05 2023-11-09 Slingshot Biosciences, Inc. Engineered particles as red blood cell mimics and compositions containing same for hematology
EP4306651A1 (en) 2022-07-10 2024-01-17 Vilnius University Composition and the use of cell lysis reagents
WO2024039763A2 (en) 2022-08-18 2024-02-22 10X Genomics, Inc. Droplet forming devices and methods having flourous diol additives
WO2024058036A1 (en) * 2022-09-14 2024-03-21 国立大学法人 鹿児島大学 Nozzle for bubble formation, bubble formation device, bubble formation method, and method for producing nozzle for bubble formation

Family Cites Families (163)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2692800A (en) 1951-10-08 1954-10-26 Gen Electric Nozzle flow control
US3980541A (en) 1967-06-05 1976-09-14 Aine Harry E Electrode structures for electric treatment of fluids and filters using same
US3816331A (en) 1972-07-05 1974-06-11 Ncr Continuous encapsulation and device therefor
CH563807A5 (en) * 1973-02-14 1975-07-15 Battelle Memorial Institute Fine granules and microcapsules mfrd. from liquid droplets - partic. of high viscosity requiring forced sepn. of droplets
CH564966A5 (en) * 1974-02-25 1975-08-15 Sauter Fr Ag Fabrik Elektrisch
US4059552A (en) 1974-06-21 1977-11-22 The Dow Chemical Company Cross-linked water-swellable polymer particles
US3982541A (en) 1974-07-29 1976-09-28 Esperance Jr Francis A L Eye surgical instrument
JPS5372016A (en) * 1976-12-08 1978-06-27 Toyo Tire & Rubber Co Ltd Apparatus for preparation and supply of heavy oil w/o emulsion fuel
US4279345A (en) 1979-08-03 1981-07-21 Allred John C High speed particle sorter using a field emission electrode
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
JPS6057907B2 (en) 1981-06-18 1985-12-17 工業技術院長 Liquid mixing and atomization method
DE3230289A1 (en) * 1982-08-14 1984-02-16 Bayer Ag, 5090 Leverkusen PRODUCTION OF PHARMACEUTICAL OR COSMETIC DISPERSIONS
US4853336A (en) 1982-11-15 1989-08-01 Technicon Instruments Corporation Single channel continuous flow system
US4618476A (en) * 1984-02-10 1986-10-21 Eastman Kodak Company Capillary transport device having speed and meniscus control means
CA1235367A (en) * 1984-04-05 1988-04-19 Gary J. Green Method and apparatus for producing uniform liquid droplets
US4865444A (en) * 1984-04-05 1989-09-12 Mobil Oil Corporation Apparatus and method for determining luminosity of hydrocarbon fuels
GB8604328D0 (en) 1986-02-21 1986-03-26 Ici Plc Producing spray of droplets of liquid
US4916070A (en) 1986-04-14 1990-04-10 The General Hospital Corporation Fibrin-specific antibodies and method of screening for the antibodies
US5204112A (en) 1986-06-16 1993-04-20 The Liposome Company, Inc. Induction of asymmetry in vesicles
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US4931225A (en) * 1987-12-30 1990-06-05 Union Carbide Industrial Gases Technology Corporation Method and apparatus for dispersing a gas into a liquid
US5093602A (en) * 1989-11-17 1992-03-03 Charged Injection Corporation Methods and apparatus for dispersing a fluent material utilizing an electron beam
JP3176607B2 (en) 1990-02-07 2001-06-18 群馬大学長 Method for forming uniform droplets
US6149789A (en) 1990-10-31 2000-11-21 Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Process for manipulating microscopic, dielectric particles and a device therefor
DE4127405C2 (en) 1991-08-19 1996-02-29 Fraunhofer Ges Forschung Process for the separation of mixtures of microscopic dielectric particles suspended in a liquid or a gel and device for carrying out the process
SE500071C2 (en) 1992-06-25 1994-04-11 Vattenfall Utveckling Ab Device for mixing two fluids, in particular liquids of different temperature
DE4308839C2 (en) * 1993-03-19 1997-04-30 Jordanow & Co Gmbh Device for mixing flow media
US5512131A (en) 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
PT687858E (en) * 1994-06-13 2001-01-31 Praxair Technology Inc LIQUID COMBUSTION COMBUSTION ATOMISTERS WITH NARROW ASPIRATION ANGLE
US5935331A (en) 1994-09-09 1999-08-10 Matsushita Electric Industrial Co., Ltd. Apparatus and method for forming films
US5762775A (en) 1994-09-21 1998-06-09 Lockheed Martin Energy Systems, Inc. Method for electrically producing dispersions of a nonconductive fluid in a conductive medium
JPH08153669A (en) 1994-11-30 1996-06-11 Hitachi Ltd Thin film forming method and formation device
EP0812434B1 (en) 1995-03-01 2013-09-18 President and Fellows of Harvard College Microcontact printing on surfaces and derivative articles
JP3232525B2 (en) 1995-08-22 2001-11-26 信越化学工業株式会社 Water repellent agent
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
JP3759986B2 (en) 1995-12-07 2006-03-29 フロイント産業株式会社 Seamless capsule and manufacturing method thereof
US5681600A (en) * 1995-12-18 1997-10-28 Abbott Laboratories Stabilization of liquid nutritional products and method of making
US5868322A (en) 1996-01-31 1999-02-09 Hewlett-Packard Company Apparatus for forming liquid droplets having a mechanically fixed inner microtube
US6355198B1 (en) 1996-03-15 2002-03-12 President And Fellows Of Harvard College Method of forming articles including waveguides via capillary micromolding and microtransfer molding
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US6116516A (en) 1996-05-13 2000-09-12 Universidad De Sevilla Stabilized capillary microjet and devices and methods for producing same
US6386463B1 (en) 1996-05-13 2002-05-14 Universidad De Sevilla Fuel injection nozzle and method of use
US6299145B1 (en) 1996-05-13 2001-10-09 Universidad De Sevilla Device and method for fluid aeration via gas forced through a liquid within an orifice of a pressure chamber
US6189803B1 (en) 1996-05-13 2001-02-20 University Of Seville Fuel injection nozzle and method of use
US6187214B1 (en) 1996-05-13 2001-02-13 Universidad De Seville Method and device for production of components for microfabrication
US6248378B1 (en) 1998-12-16 2001-06-19 Universidad De Sevilla Enhanced food products
US6196525B1 (en) 1996-05-13 2001-03-06 Universidad De Sevilla Device and method for fluid aeration via gas forced through a liquid within an orifice of a pressure chamber
US6405936B1 (en) 1996-05-13 2002-06-18 Universidad De Sevilla Stabilized capillary microjet and devices and methods for producing same
ES2140998B1 (en) 1996-05-13 2000-10-16 Univ Sevilla LIQUID ATOMIZATION PROCEDURE.
EP0907412B1 (en) 1996-06-28 2008-08-27 Caliper Life Sciences, Inc. High-throughput screening assay systems in microscale fluidic devices
US6252129B1 (en) 1996-07-23 2001-06-26 Electrosols, Ltd. Dispensing device and method for forming material
US6143248A (en) * 1996-08-12 2000-11-07 Gamera Bioscience Corp. Capillary microvalve
CA2264389A1 (en) 1996-09-04 1998-03-12 Technical University Of Denmark A micro flow system for particle separation and analysis
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US6120666A (en) 1996-09-26 2000-09-19 Ut-Battelle, Llc Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same
JPH10217477A (en) 1997-02-07 1998-08-18 Fuji Xerox Co Ltd Ink jet recording device
DK1019496T3 (en) 1997-07-07 2005-01-10 Medical Res Council In vitro sorting method
US5980936A (en) 1997-08-07 1999-11-09 Alliance Pharmaceutical Corp. Multiple emulsions comprising a hydrophobic continuous phase
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
HU224664B1 (en) * 1997-09-25 2005-12-28 Ge Bayer Silicones Gmbh & Co.Kg, Device and method for producing silicone emulsions
JP3081880B2 (en) * 1998-03-30 2000-08-28 農林水産省食品総合研究所長 Microsphere continuous manufacturing equipment
EP1190229B1 (en) 1998-05-22 2011-10-26 California Institute Of Technology Microfabricated cell sorter
US6003794A (en) 1998-08-04 1999-12-21 Progressive Grower Technologies, Inc. Electrostatic spray module
US6614598B1 (en) 1998-11-12 2003-09-02 Institute Of Technology, California Microlensing particles and applications
US6450189B1 (en) 1998-11-13 2002-09-17 Universidad De Sevilla Method and device for production of components for microfabrication
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6633031B1 (en) 1999-03-02 2003-10-14 Advion Biosciences, Inc. Integrated monolithic microfabricated dispensing nozzle and liquid chromatography-electrospray system and method
DE19911777A1 (en) * 1999-03-17 2000-09-21 Merck Patent Gmbh Process for the preparation of cosmetic formulations
US6592821B1 (en) 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
CA2373347A1 (en) 1999-05-17 2000-11-23 Caliper Technologies Corporation Focusing of microparticles in microfluidic systems
US20060169800A1 (en) * 1999-06-11 2006-08-03 Aradigm Corporation Aerosol created by directed flow of fluids and devices and methods for producing same
ES2424713T4 (en) * 1999-06-11 2014-01-23 Aradigm Corporation Method of producing a spray
MXPA01012959A (en) 1999-06-28 2002-07-30 California Inst Of Techn Microfabricated elastomeric valve and pump systems.
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US20010050881A1 (en) 1999-09-20 2001-12-13 Depaoli David W. Continuous flow, electrohydrodynamic micromixing apparatus and methods
US6890487B1 (en) * 1999-09-30 2005-05-10 Science & Technology Corporation ©UNM Flow cytometry for high throughput screening
DE19961257C2 (en) 1999-12-18 2002-12-19 Inst Mikrotechnik Mainz Gmbh micromixer
AU2001232805A1 (en) 2000-01-12 2001-07-24 Ut-Battelle, Llc A microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream
MXPA02008787A (en) * 2000-03-10 2004-03-26 Flow Focusing Inc Methods for producing optical fiber by focusing high viscosity liquid.
US7485454B1 (en) * 2000-03-10 2009-02-03 Bioprocessors Corp. Microreactor
DE10015109A1 (en) * 2000-03-28 2001-10-04 Peter Walzel Processes and devices for producing drops of equal size
AU2001255458A1 (en) 2000-04-18 2001-10-30 Waters Investments Limited Improved electrospray and other lc/ms interfaces
JP2001301154A (en) 2000-04-20 2001-10-30 Dainippon Printing Co Ltd Field jet sticking method of liquid having surface tension lowering upon application of voltage
DE10025290B4 (en) 2000-05-22 2005-03-24 Fico I.T.M. S.A. Sun visor outer surfaces
US20010048637A1 (en) * 2000-05-24 2001-12-06 Weigl Bernhard H. Microfluidic system and method
US6686184B1 (en) 2000-05-25 2004-02-03 President And Fellows Of Harvard College Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US6777450B1 (en) 2000-05-26 2004-08-17 Color Access, Inc. Water-thin emulsions with low emulsifier levels
US20060263888A1 (en) 2000-06-02 2006-11-23 Honeywell International Inc. Differential white blood count on a disposable card
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6301055B1 (en) 2000-08-16 2001-10-09 California Institute Of Technology Solid immersion lens structures and methods for producing solid immersion lens structures
DE10041823C2 (en) * 2000-08-25 2002-12-19 Inst Mikrotechnik Mainz Gmbh Method and static micromixer for mixing at least two fluids
US6610499B1 (en) 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
EP1334347A1 (en) * 2000-09-15 2003-08-13 California Institute Of Technology Microfabricated crossflow devices and methods
US6508988B1 (en) 2000-10-03 2003-01-21 California Institute Of Technology Combinatorial synthesis system
US6778724B2 (en) 2000-11-28 2004-08-17 The Regents Of The University Of California Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices
US20040096515A1 (en) 2001-12-07 2004-05-20 Bausch Andreas R. Methods and compositions for encapsulating active agents
WO2002047665A2 (en) 2000-12-07 2002-06-20 President And Fellows Of Harvard College Methods and compositions for encapsulating active agents
WO2002060275A1 (en) 2001-01-31 2002-08-08 Kraft Foods Holdings, Inc. Production of capsules and particles for improvement of food products
ES2180405B1 (en) 2001-01-31 2004-01-16 Univ Sevilla DEVICE AND PROCEDURE FOR PRODUCING MULTICOMPONENT COMPOSITE LIQUID JEANS AND MULTICOMPONENT AND / OR MULTI-PAPER MICRO AND NANOMETRIC SIZE CAPSULES.
EP1741482B1 (en) 2001-02-23 2008-10-15 Japan Science and Technology Agency Process and apparatus for producing microcapsules
EP1362634B1 (en) 2001-02-23 2006-05-31 Japan Science and Technology Agency Process for producing emulsion and apparatus therefor
US7037417B2 (en) * 2001-03-19 2006-05-02 Ecole Polytechnique Federale De Lausanne Mechanical control of fluids in micro-analytical devices
US6752922B2 (en) 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
US7318642B2 (en) 2001-04-10 2008-01-15 Essilor International (Compagnie Générale d'Optique) Progressive addition lenses with reduced unwanted astigmatism
JP4194934B2 (en) 2001-05-26 2008-12-10 ワン セル システムズ インコーポレイテッド Protein secretion by encapsulated cells
GB0114854D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
US20030015425A1 (en) 2001-06-20 2003-01-23 Coventor Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
EP1412065A2 (en) 2001-07-27 2004-04-28 President And Fellows Of Harvard College Laminar mixing apparatus and methods
US6520425B1 (en) 2001-08-21 2003-02-18 The University Of Akron Process and apparatus for the production of nanofibers
JP4182195B2 (en) * 2001-09-03 2008-11-19 独立行政法人農業・食品産業技術総合研究機構 Monodispersed complex emulsion production equipment
US7147763B2 (en) 2002-04-01 2006-12-12 Palo Alto Research Center Incorporated Apparatus and method for using electrostatic force to cause fluid movement
US6976590B2 (en) 2002-06-24 2005-12-20 Cytonome, Inc. Method and apparatus for sorting particles
EP2282214B1 (en) * 2002-05-09 2022-10-05 The University of Chicago Device and method for pressure-driven plug transport and reaction
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
US7329545B2 (en) 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
GB2395196B (en) 2002-11-14 2006-12-27 Univ Cardiff Microfluidic device and methods for construction and application
US7595195B2 (en) 2003-02-11 2009-09-29 The Regents Of The University Of California Microfluidic devices for controlled viscous shearing and formation of amphiphilic vesicles
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US7045040B2 (en) 2003-03-20 2006-05-16 Asm Nutool, Inc. Process and system for eliminating gas bubbles during electrochemical processing
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
WO2004091763A2 (en) 2003-04-10 2004-10-28 President And Fellows Of Harvard College Formation and control of fluidic species
EP1629286A1 (en) 2003-05-16 2006-03-01 Global Technologies (NZ) Ltd. Method and apparatus for mixing sample and reagent in a suspension fluid
WO2004103565A2 (en) 2003-05-19 2004-12-02 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Device and method for structuring liquids and for dosing reaction liquids into liquid compartments immersed in a separation medium
JP2005037346A (en) 2003-06-25 2005-02-10 Aisin Seiki Co Ltd Micro fluid control system
US7115230B2 (en) 2003-06-26 2006-10-03 Intel Corporation Hydrodynamic focusing devices
GB0315438D0 (en) 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
US20050032238A1 (en) 2003-08-07 2005-02-10 Nanostream, Inc. Vented microfluidic separation devices and methods
KR20070029618A (en) 2003-08-27 2007-03-14 더 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Electronic control of fluidic species
CA2536360C (en) 2003-08-28 2013-08-06 Celula, Inc. Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
US7204431B2 (en) 2003-10-31 2007-04-17 Agilent Technologies, Inc. Electrospray ion source for mass spectroscopy
EP1533605A3 (en) 2003-11-19 2006-05-31 Aisin Seiki Kabushiki Kaisha Micro control system for transfer of liquids
WO2005049787A2 (en) 2003-11-24 2005-06-02 Yeda Research And Development Co.Ltd. Compositions and methods for in vitro sorting of molecular and cellular libraries
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US8696952B2 (en) 2004-04-23 2014-04-15 Eugenia Kumacheva Method of producing polymeric particles with selected size, shape, morphology and composition
WO2006002641A1 (en) 2004-07-02 2006-01-12 Versamatrix A/S Spherical radiofrequency-encoded beads
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7759111B2 (en) 2004-08-27 2010-07-20 The Regents Of The University Of California Cell encapsulation microfluidic device
US9566558B2 (en) 2004-09-09 2017-02-14 Institut Curie Device for manipulation of packets in micro-containers, in particular in microchannels
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20080004436A1 (en) 2004-11-15 2008-01-03 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed Evolution and Selection Using in Vitro Compartmentalization
US20080213593A1 (en) 2005-01-21 2008-09-04 President And Fellows Of Harvard College Systems And Methods For Forming Fluidic Droplets Encapsulated In Particles Such As Colloidal Particles
CA2599683A1 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US20070054119A1 (en) 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
FR2882939B1 (en) * 2005-03-11 2007-06-08 Centre Nat Rech Scient FLUIDIC SEPARATION DEVICE
US8734003B2 (en) 2005-09-15 2014-05-27 Alcatel Lucent Micro-chemical mixing
WO2007081387A1 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices, methods of use, and kits for performing diagnostics
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
AU2007210152A1 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
WO2007114794A1 (en) 2006-03-31 2007-10-11 Nam Trung Nguyen Active control for droplet-based microfluidics
US20080003142A1 (en) 2006-05-11 2008-01-03 Link Darren R Microfluidic devices
FR2901717A1 (en) 2006-05-30 2007-12-07 Centre Nat Rech Scient METHOD FOR TREATING DROPS IN A MICROFLUIDIC CIRCUIT
US7776927B2 (en) 2007-03-28 2010-08-17 President And Fellows Of Harvard College Emulsions and techniques for formation
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
US20090068170A1 (en) 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
JP2010198393A (en) 2009-02-26 2010-09-09 Alpine Electronics Inc Map display device
WO2012048341A1 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding

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