WO2003083477A3 - Active solid support and method for surface immobilization of combinatorial compounds or libraries - Google Patents
Active solid support and method for surface immobilization of combinatorial compounds or libraries Download PDFInfo
- Publication number
- WO2003083477A3 WO2003083477A3 PCT/HU2003/000025 HU0300025W WO03083477A3 WO 2003083477 A3 WO2003083477 A3 WO 2003083477A3 HU 0300025 W HU0300025 W HU 0300025W WO 03083477 A3 WO03083477 A3 WO 03083477A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- solid support
- group
- active solid
- reacted
- drug
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 7
- 239000007787 solid Substances 0.000 title abstract 7
- 150000001875 compounds Chemical class 0.000 title 1
- 229940079593 drug Drugs 0.000 abstract 3
- 239000003814 drug Substances 0.000 abstract 3
- 125000000217 alkyl group Chemical group 0.000 abstract 2
- 238000004873 anchoring Methods 0.000 abstract 2
- 125000003710 aryl alkyl group Chemical group 0.000 abstract 2
- WERYXYBDKMZEQL-UHFFFAOYSA-N butane-1,4-diol Chemical compound OCCCCO WERYXYBDKMZEQL-UHFFFAOYSA-N 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 229940000406 drug candidate Drugs 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 238000002493 microarray Methods 0.000 abstract 2
- 239000002516 radical scavenger Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- JHWGFJBTMHEZME-UHFFFAOYSA-N 4-prop-2-enoyloxybutyl prop-2-enoate Chemical compound C=CC(=O)OCCCCOC(=O)C=C JHWGFJBTMHEZME-UHFFFAOYSA-N 0.000 abstract 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 abstract 1
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 150000007513 acids Chemical class 0.000 abstract 1
- 125000003647 acryloyl group Chemical group O=C([*])C([H])=C([H])[H] 0.000 abstract 1
- 125000003277 amino group Chemical group 0.000 abstract 1
- 125000003118 aryl group Chemical group 0.000 abstract 1
- 125000004432 carbon atom Chemical group C* 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 125000003700 epoxy group Chemical group 0.000 abstract 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 1
- 125000000524 functional group Chemical group 0.000 abstract 1
- 229910052736 halogen Inorganic materials 0.000 abstract 1
- 150000002367 halogens Chemical class 0.000 abstract 1
- NHBRUUFBSBSTHM-UHFFFAOYSA-N n'-[2-(3-trimethoxysilylpropylamino)ethyl]ethane-1,2-diamine Chemical compound CO[Si](OC)(OC)CCCNCCNCCN NHBRUUFBSBSTHM-UHFFFAOYSA-N 0.000 abstract 1
- 238000002203 pretreatment Methods 0.000 abstract 1
- 125000003107 substituted aryl group Chemical group 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The subject of the invention is a method for production of an active solid support suitable for anchoring drug molecules through covalent bonds, using the following methods a.) a solid support prepared in advance, preferably by pre-treatment in the presence of strong bases or acids, then reacted with a polyamine-functionalized alcoxy-silane derivative, preferably 3-[2-(2-aminoethylamino)ethylamino]propyl trimethoxy-silane, followed by washing and drying, b1) the solid support obtained in the procedure described above in Section a.) will be reacted with (I) where Z: = bond or a -CO or -CNR 1 group and group 121 is an alkyl, aralkyl or aryl group; B: = bond or a -(CH2)n- or -(CH2)n O-(CH2)n or O-(CH2)n- or -NH(CH2)n- group; A: = a branched or unbranched alkyl or substituted aryl or aralkyl group containing 1-5 carbon atoms; D: = a halogen or -O-A group and n = 0, 1, 2, ... integer - with a bi-functional reagent, preferably reacted with acryloyl/chloride or 1,4-butanediol diacrylate in the presence of an organic or inorganic scavenger or b2) the support prepared using the method described in part (a) is reacted with a bi-functional reagent, preferably 1,4-butanediol diglicidyl-ether or epichlorohydrin, in the presence of an organic or inorganic scavenger containing terminal epoxy groups and an amino-group reactive functional subgroup. The invention also includes the active solid support produced using the several methods described above, as well as application of the active solid support described above for production of chemical microarrays and combinatorial chemical microarrays. The invention further comprises a method for immobilization of what have been termed organic molecules of low molecular weight, having a size less than 1500 Da, preferably between 250 and 750 Da. This is achieved by anchoring an unmodified drug or drug candidate molecule to the active solid support described above by adding the appropriate functional groups of the drug or drug candidate molecule, preferably executed using a micron-sized robotized binding technique.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP03745343A EP1493031A2 (en) | 2002-03-29 | 2003-03-28 | Active solid support and method for surface immobilization of combinatorial compounds or libraries |
| AU2003226574A AU2003226574A1 (en) | 2002-03-29 | 2003-03-28 | Active solid support and method for surface immobilization of combinatorial compounds or libraries |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HUP0201091 | 2002-03-29 | ||
| HU0201091A HU226993B1 (en) | 2002-03-29 | 2002-03-29 | Novel active carrier and process for immobilization of combinatorial compounds and compound libraries |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2003083477A2 WO2003083477A2 (en) | 2003-10-09 |
| WO2003083477A3 true WO2003083477A3 (en) | 2004-05-06 |
Family
ID=89980305
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/HU2003/000025 WO2003083477A2 (en) | 2002-03-29 | 2003-03-28 | Active solid support and method for surface immobilization of combinatorial compounds or libraries |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1493031A2 (en) |
| AU (1) | AU2003226574A1 (en) |
| HU (1) | HU226993B1 (en) |
| WO (1) | WO2003083477A2 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7217512B2 (en) | 2002-05-09 | 2007-05-15 | Corning Incorporated | Reagent and method for attaching target molecules to a surface |
| US7723126B2 (en) * | 2004-03-24 | 2010-05-25 | Wisconsin Alumni Research Foundation | Plasma-enhanced functionalization of inorganic oxide surfaces |
| US20060147943A1 (en) * | 2004-12-30 | 2006-07-06 | Lewis Mark A | Substrates having pendant epoxide groups for binding biomolecules and methods of making and using thereof |
| US7396676B2 (en) | 2005-05-31 | 2008-07-08 | Agilent Technologies, Inc. | Evanescent wave sensor with attached ligand |
| US20070099180A1 (en) * | 2005-10-31 | 2007-05-03 | Robotti Karla M | Evanescent wave sensor with attached ligand |
| HUP0600668A2 (en) | 2006-08-22 | 2008-02-28 | Avicor Kft | Active carrier, process for producing thereof and the use of thereof |
| US8029902B2 (en) | 2006-12-11 | 2011-10-04 | Wisconsin Alumni Research Foundation | Plasma-enhanced functionalization of substrate surfaces with quaternary ammonium and quaternary phosphonium groups |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5919626A (en) * | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
| US5919523A (en) * | 1995-04-27 | 1999-07-06 | Affymetrix, Inc. | Derivatization of solid supports and methods for oligomer synthesis |
| WO2001001143A2 (en) * | 1999-06-25 | 2001-01-04 | Motorola Inc. | Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker |
-
2002
- 2002-03-29 HU HU0201091A patent/HU226993B1/en not_active IP Right Cessation
-
2003
- 2003-03-28 AU AU2003226574A patent/AU2003226574A1/en not_active Abandoned
- 2003-03-28 EP EP03745343A patent/EP1493031A2/en not_active Withdrawn
- 2003-03-28 WO PCT/HU2003/000025 patent/WO2003083477A2/en not_active Application Discontinuation
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5919523A (en) * | 1995-04-27 | 1999-07-06 | Affymetrix, Inc. | Derivatization of solid supports and methods for oligomer synthesis |
| US5919626A (en) * | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
| WO2001001143A2 (en) * | 1999-06-25 | 2001-01-04 | Motorola Inc. | Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker |
Non-Patent Citations (2)
| Title |
|---|
| BLACKBURN CHRISTOPHER: "Polymer supports for solid-phase organic synthesis.", BIOPOLYMERS, vol. 47, no. 5, 25 January 1998 (1998-01-25), pages 311 - 351, XP002255078, ISSN: 0006-3525 * |
| REHMAN F ET AL: "Immobilization of acrylamide-modified oligonucleotides by co-polymerization", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 2, 15 February 1999 (1999-02-15), pages 649 - 655, XP002155808, ISSN: 0305-1048 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003226574A8 (en) | 2003-10-13 |
| WO2003083477A2 (en) | 2003-10-09 |
| HU0201091D0 (en) | 2002-06-29 |
| AU2003226574A1 (en) | 2003-10-13 |
| EP1493031A2 (en) | 2005-01-05 |
| HU226993B1 (en) | 2010-04-28 |
| HUP0201091A2 (en) | 2003-12-29 |
| HUP0201091A3 (en) | 2007-08-28 |
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