WO2003074659A3 - Magnetic immobilization of cells - Google Patents

Magnetic immobilization of cells Download PDF

Info

Publication number
WO2003074659A3
WO2003074659A3 PCT/US2003/005841 US0305841W WO03074659A3 WO 2003074659 A3 WO2003074659 A3 WO 2003074659A3 US 0305841 W US0305841 W US 0305841W WO 03074659 A3 WO03074659 A3 WO 03074659A3
Authority
WO
WIPO (PCT)
Prior art keywords
magnetic
substrate
magnetic field
localized
receptacle
Prior art date
Application number
PCT/US2003/005841
Other languages
French (fr)
Other versions
WO2003074659A2 (en
Inventor
Rocco Casagrade
Evelyn Wang
Gregory Kirk
Michael Nussbaum
Enoch Kim
Aaron Raphel
Original Assignee
Surface Logix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/084,063 external-priority patent/US7285412B2/en
Application filed by Surface Logix Inc filed Critical Surface Logix Inc
Priority to AU2003217743A priority Critical patent/AU2003217743A1/en
Publication of WO2003074659A2 publication Critical patent/WO2003074659A2/en
Publication of WO2003074659A3 publication Critical patent/WO2003074659A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00655—Making arrays on substantially continuous surfaces the compounds being bound to magnets embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/0074—Biological products
    • B01J2219/00743—Cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation

Abstract

The present invention relates to an apparatus and methods that immobilize one or more cells associated with magnetic material on a substrate on which are located one or more magnetic receptacle(s). Alternatively, in another aspect the present invention, the device arrays cells associated with magnetic material on a substrate having a pattern of magnetic receptacles disposed thereon. The size of the magnetic receptacle(s) determines the number of target cells that it is capable of immobilizing. The size of the magnetic receptacle is defined by the strength of a localized magnetic field gradient. The localized magnetic field gradient may be derived from 1) permanent magnets embedded in the substrate or alternatively, the localized magnetic field gradient may be derived from an 2) external magnet whose strength is focused by objects of highly-permeable-magnetic material which create localized magnetic field gradients. The invention apparatus comprises a removable cell delivery device and a substrate, which has one or more magnetic receptacles disposed thereon.
PCT/US2003/005841 2002-02-28 2003-02-27 Magnetic immobilization of cells WO2003074659A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003217743A AU2003217743A1 (en) 2002-02-28 2003-02-27 Magnetic immobilization of cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/084,063 US7285412B2 (en) 2001-07-27 2002-02-28 Device for magnetic immobilization of cells
US10/084,063 2002-02-28

Publications (2)

Publication Number Publication Date
WO2003074659A2 WO2003074659A2 (en) 2003-09-12
WO2003074659A3 true WO2003074659A3 (en) 2007-03-15

Family

ID=27787458

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/005841 WO2003074659A2 (en) 2002-02-28 2003-02-27 Magnetic immobilization of cells

Country Status (2)

Country Link
AU (1) AU2003217743A1 (en)
WO (1) WO2003074659A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006036380A1 (en) 2006-08-02 2008-02-07 Universität des Saarlandes Method for influencing living cells by cell-surface interaction
JP6037701B2 (en) * 2012-08-03 2016-12-07 株式会社日立ハイテクノロジーズ Immune analyzer
CN104897300B (en) * 2015-05-18 2017-05-31 宁波大学 The method that noncontact utilizes fluorescence measurement ferroelectric Curie temperature
US11039413B2 (en) * 2017-05-05 2021-06-15 Idac Holdings, Inc. Methods, apparatus and systems for supporting mobile initiated connection only (MICO) wireless transmit/receive units (WTRUs)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5567326A (en) * 1994-09-19 1996-10-22 Promega Corporation Multisample magnetic separation device
US5968820A (en) * 1997-02-26 1999-10-19 The Cleveland Clinic Foundation Method for magnetically separating cells into fractionated flow streams
US6013532A (en) * 1990-09-26 2000-01-11 Immunivest Corporation Methods for magnetic immobilization and manipulation of cells
US6136182A (en) * 1996-06-07 2000-10-24 Immunivest Corporation Magnetic devices and sample chambers for examination and manipulation of cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013532A (en) * 1990-09-26 2000-01-11 Immunivest Corporation Methods for magnetic immobilization and manipulation of cells
US5567326A (en) * 1994-09-19 1996-10-22 Promega Corporation Multisample magnetic separation device
US6136182A (en) * 1996-06-07 2000-10-24 Immunivest Corporation Magnetic devices and sample chambers for examination and manipulation of cells
US5968820A (en) * 1997-02-26 1999-10-19 The Cleveland Clinic Foundation Method for magnetically separating cells into fractionated flow streams

Also Published As

Publication number Publication date
AU2003217743A8 (en) 2003-09-16
AU2003217743A1 (en) 2003-09-16
WO2003074659A2 (en) 2003-09-12

Similar Documents

Publication Publication Date Title
FI980399A (en) Method and means for transferring the substance
EP0829900A3 (en) Plasma process device
EP1106709A3 (en) Pulsed-mode RF bias for side-wall coverage improvement
EP1465211A3 (en) Printed high strength permanent magnet targets for magnetic sensor
ATE328065T1 (en) ARTIFICIALLY PRODUCED THREE-DIMENSIONAL MAMMAL HEART MUSCLE TISSUE AND MANUFACTURING METHOD
EP2500076A3 (en) Structure and method for handling magnetic particles in biological assays
WO2002073228A3 (en) Method for high resolution magnetic resonance analysis using magic angle technique
EP1647831A3 (en) Open superconducting magnet apparatus
IL177335A0 (en) Magnetic manipulation of biological samples
DE60334815D1 (en) SYSTEM FOR DIAMAGNETIC LEVITATION
MY122866A (en) Apparatus and method for testing semiconductor devices
EP1124310A3 (en) Two-dimensional electric motor, exposure apparatus and semiconductor fabrication method
CN104851828A (en) Universal process carrier for substrates
DE60043871D1 (en) Surface treatment method, surface treatment apparatus, vapor deposition material, and rare earth based permanent magnet with treated surface
WO2002000870A3 (en) Method for transfecting cells using a magnetic field
EP1780601A3 (en) Lithographic apparatus and device manufacturing method
EP0887655A3 (en) Open MRI magnet with inhomogeneous field
WO2003074659A3 (en) Magnetic immobilization of cells
DE3886754D1 (en) Device for plasma or reactive ion etching and method for etching poorly heat-conducting substrates.
ATE404269T1 (en) METHOD AND DEVICE FOR MAGNETIC SEPARATION
WO2001092906A3 (en) Method and apparatus for determining the pass through flux of magnetic materials
JP2006006936A (en) Magnetic field generating device and shimming method therefor
DE19981147T1 (en) Thin film processing electromagnet for low-distortion magnetic orientation
AU7269500A (en) Diaphragm transducer
WO1998041319A3 (en) Device for magnetic fluid treatment

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SK SL TJ TM TN TR TT TZ UA UG UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP