WO2003070875A3 - Behälter mit zumindest einer elektrode - Google Patents

Behälter mit zumindest einer elektrode Download PDF

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Publication number
WO2003070875A3
WO2003070875A3 PCT/DE2003/000536 DE0300536W WO03070875A3 WO 2003070875 A3 WO2003070875 A3 WO 2003070875A3 DE 0300536 W DE0300536 W DE 0300536W WO 03070875 A3 WO03070875 A3 WO 03070875A3
Authority
WO
WIPO (PCT)
Prior art keywords
container
electrode
receiving
solution
conductive
Prior art date
Application number
PCT/DE2003/000536
Other languages
English (en)
French (fr)
Other versions
WO2003070875B1 (de
WO2003070875A2 (de
Inventor
Herbert Mueller-Hartmann
Michael Habig
Gregor Siebenkotten
Peter Hoffmann
Original Assignee
Amaxa Gmbh
Herbert Mueller-Hartmann
Michael Habig
Gregor Siebenkotten
Peter Hoffmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amaxa Gmbh, Herbert Mueller-Hartmann, Michael Habig, Gregor Siebenkotten, Peter Hoffmann filed Critical Amaxa Gmbh
Priority to AT03742495T priority Critical patent/ATE299528T1/de
Priority to AU2003227006A priority patent/AU2003227006B2/en
Priority to EP03742495A priority patent/EP1476537B1/de
Priority to CA2476439A priority patent/CA2476439C/en
Priority to JP2003569768A priority patent/JP4369241B2/ja
Priority to IL16365603A priority patent/IL163656A0/xx
Priority to DE50300761T priority patent/DE50300761D1/de
Priority to KR1020047012997A priority patent/KR100852288B1/ko
Priority to US10/505,149 priority patent/US7678564B2/en
Publication of WO2003070875A2 publication Critical patent/WO2003070875A2/de
Publication of WO2003070875A3 publication Critical patent/WO2003070875A3/de
Publication of WO2003070875B1 publication Critical patent/WO2003070875B1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

Die Erfindung betrifft einen Behälter 20, 30 zur Aufnahme einer wässrigen Lösung, welcher zumindest teilweise von einer äußeren Begrenzung 21 gebildet ist, der einen Innenraum 22, 32 zur Aufnahme der Lösung bildet, und welcher mindestens einen Bereich aufweist, der beim Anlegen einer elektrischen Spannung und einer anschließenden Entladung als Elektrode 25, 26, 33, 34 dient, wobei mindestens eine Elektrode 25, 26, 33, 34 aus einem leitfähigen Kunststoffmaterial besteht, das zumindest auf einem Kunststoff basiert, der mit mindestens einem leitfähigen Stoff dotiert ist. Hierdurch wurde ein Behälter 20, 30 der eingangs genannten Art geschaffen, der einfach und kostengünstig herzustellen ist und darüber hinaus beispielsweise eine effiziente Transfektion von lebenden Zellen mittels Elektroporation oder eine effektive Elektrofusion ermöglicht.
PCT/DE2003/000536 2002-02-20 2003-02-20 Behälter mit zumindest einer elektrode WO2003070875A2 (de)

Priority Applications (9)

Application Number Priority Date Filing Date Title
AT03742495T ATE299528T1 (de) 2002-02-20 2003-02-20 Behälter mit zumindest einer elektrode
AU2003227006A AU2003227006B2 (en) 2002-02-20 2003-02-20 Container with at least one electrode
EP03742495A EP1476537B1 (de) 2002-02-20 2003-02-20 Behälter mit zumindest einer elektrode
CA2476439A CA2476439C (en) 2002-02-20 2003-02-20 Container with at least one electrode
JP2003569768A JP4369241B2 (ja) 2002-02-20 2003-02-20 少なくとも１つの電極をもつ容器
IL16365603A IL163656A0 (en) 2002-02-20 2003-02-20 Container with at least one electrode
DE50300761T DE50300761D1 (de) 2002-02-20 2003-02-20 Behälter mit zumindest einer elektrode
KR1020047012997A KR100852288B1 (ko) 2002-02-20 2003-02-20 적어도 하나의 전극을 구비하는 컨테이너
US10/505,149 US7678564B2 (en) 2002-02-20 2003-02-20 Container with at least one electrode

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10208188A DE10208188B4 (de) 2002-02-20 2002-02-20 Behälter mit zumindest einer Elektrode
DE10208188.3 2002-02-20

Publications (3)

Publication Number Publication Date
WO2003070875A2 WO2003070875A2 (de) 2003-08-28
WO2003070875A3 true WO2003070875A3 (de) 2004-01-08
WO2003070875B1 WO2003070875B1 (de) 2004-03-25

Family

ID=27674978

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2003/000536 WO2003070875A2 (de) 2002-02-20 2003-02-20 Behälter mit zumindest einer elektrode

Country Status (11)

Country Link
US (1) US7678564B2 (de)
EP (1) EP1476537B1 (de)
JP (1) JP4369241B2 (de)
KR (1) KR100852288B1 (de)
CN (1) CN1312275C (de)
AT (1) ATE299528T1 (de)
AU (1) AU2003227006B2 (de)
CA (1) CA2476439C (de)
DE (2) DE10208188B4 (de)
IL (1) IL163656A0 (de)
WO (1) WO2003070875A2 (de)

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US6897069B1 (en) * 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
CN103060191B (zh) 2004-06-12 2015-03-11 英杰生命新加坡私人有限公司 一种电穿孔系统
US7850645B2 (en) 2005-02-11 2010-12-14 Boston Scientific Scimed, Inc. Internal medical devices for delivery of therapeutic agent in conjunction with a source of electrical power
US8101169B2 (en) * 2005-02-23 2012-01-24 The Board Of Trustees Of The Leland Stanford Junior University Ocular gene therapy using avalanche-mediated transfection
US7923251B2 (en) * 2005-02-23 2011-04-12 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus for avalanche-mediated transfer of agents into cells
US7704743B2 (en) * 2005-03-30 2010-04-27 Georgia Tech Research Corporation Electrosonic cell manipulation device and method of use thereof
DE112006003105T5 (de) * 2005-11-15 2008-10-09 Credant Technologies, Inc., Addison System und Verfahren für die sichere, transparente und kontinuierliche Synchronisierung von Zugriffsbeglaubigungen in einem beliebigen System einer dritten Seite
WO2008102728A1 (ja) * 2007-02-19 2008-08-28 Kyoto University 核酸導入用導電性基板および核酸導入方法
US8043838B2 (en) * 2008-02-20 2011-10-25 Bio-Rad Laboratories, Inc. Electroporation cuvette with spatially variable electric field
KR101084528B1 (ko) 2008-04-15 2011-11-18 인비트로겐 싱가포르 피티이. 엘티디. 전기천공 장치용 파이펫 팁
DE102008039757A1 (de) * 2008-08-20 2010-02-25 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Aktorelement sowie seine Verwendung
DE102009049783A1 (de) 2009-10-19 2011-04-21 Eppendorf Ag Elektrisch leitfähige Pipettenspitze
WO2012098260A1 (en) 2011-01-21 2012-07-26 Axiogenesis Ag A non-viral system for the generation of induced pluripotent stem (ips) cells
US9382510B2 (en) 2011-08-25 2016-07-05 Jian Chen Methods and devices for electroporation
US10947526B2 (en) 2014-07-03 2021-03-16 Massachusetts Institute Of Technology Microfluidic assay for rapid optimization of cell electroporation
CN109679844B (zh) * 2017-10-19 2023-08-22 苏州壹达生物科技有限公司 一种流式电穿孔装置
US20210231637A1 (en) * 2018-04-27 2021-07-29 Ion Chat Research Corporate Method for measuring intracellular potential with a capacitance type potential measurement device
CN110448293A (zh) * 2019-08-05 2019-11-15 苏州米特希赛尔人工智能有限公司 一种心电图检测干电极

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US6040184A (en) * 1998-10-09 2000-03-21 Stratagene Method for more efficient electroporation
WO2001070928A1 (en) * 2000-03-21 2001-09-27 Skwarczuk, Vanda Method and apparatus for electroporation of cells using electrical pulses of long duration

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Also Published As

Publication number Publication date
US7678564B2 (en) 2010-03-16
DE50300761D1 (de) 2005-08-18
DE10208188A1 (de) 2003-09-04
IL163656A0 (en) 2005-12-18
CA2476439A1 (en) 2003-08-28
JP4369241B2 (ja) 2009-11-18
CN1643132A (zh) 2005-07-20
WO2003070875B1 (de) 2004-03-25
KR100852288B1 (ko) 2008-08-14
EP1476537B1 (de) 2005-07-13
AU2003227006B8 (en) 2003-09-09
DE10208188B4 (de) 2006-05-24
WO2003070875A2 (de) 2003-08-28
CN1312275C (zh) 2007-04-25
EP1476537A2 (de) 2004-11-17
ATE299528T1 (de) 2005-07-15
AU2003227006A1 (en) 2003-09-09
KR20040085211A (ko) 2004-10-07
CA2476439C (en) 2010-04-20
US20050064578A1 (en) 2005-03-24
JP2005526499A (ja) 2005-09-08
AU2003227006B2 (en) 2007-07-26

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