WO2003064991A3 - Method and apparatus using a nonlinear optical technique for detection of probe-target interactions in a field - Google Patents

Method and apparatus using a nonlinear optical technique for detection of probe-target interactions in a field Download PDF

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Publication number
WO2003064991A3
WO2003064991A3 PCT/US2002/022681 US0222681W WO03064991A3 WO 2003064991 A3 WO2003064991 A3 WO 2003064991A3 US 0222681 W US0222681 W US 0222681W WO 03064991 A3 WO03064991 A3 WO 03064991A3
Authority
WO
WIPO (PCT)
Prior art keywords
nonlinear
probe
optical technique
nonlinear optical
external force
Prior art date
Application number
PCT/US2002/022681
Other languages
French (fr)
Other versions
WO2003064991A2 (en
Inventor
Joshua S Salafsky
Original Assignee
Joshua S Salafsky
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joshua S Salafsky filed Critical Joshua S Salafsky
Priority to AU2002365214A priority Critical patent/AU2002365214A1/en
Publication of WO2003064991A2 publication Critical patent/WO2003064991A2/en
Publication of WO2003064991A3 publication Critical patent/WO2003064991A3/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

Abstract

A nonlinear optical technique, such as second or third harmonic or sum or difference frequency generation, is used to detect binding interactions, or the degree or extent of binding, in an applied external force field. The external field is used to create non-centrosymmetric region in a sample comprising the probes and/or targets. Targets and probes can be labeled with non-linear-active moieties, or modulators of the nonlinear active properties of these moieties. In one aspect of the present invention, the nonlinear optical technique detects a change in nonlinear activity in an applied external force field of a probe due to target binding. In another aspect of the invention, the nonlinear optical technique screens candidate probes by detecting a change in nonlinear activity in an applied external force field due to a probe-target interaction. In another aspect of the invention, the nonlinear optical technique screens candidate modulators of a probe-target interaction by detecting a change in nonlinear activity in an applied external force field in the presence of the modulator.
PCT/US2002/022681 2001-07-17 2002-07-17 Method and apparatus using a nonlinear optical technique for detection of probe-target interactions in a field WO2003064991A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002365214A AU2002365214A1 (en) 2001-07-17 2002-07-17 Method and apparatus using a nonlinear optical technique for detection of probe-target interactions in a field

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US30604001P 2001-07-17 2001-07-17
US60/306,040 2001-07-17
US34782101P 2001-10-23 2001-10-23
US60/347,821 2001-10-23
US35466802P 2002-02-06 2002-02-06
US60/354,668 2002-02-06

Publications (2)

Publication Number Publication Date
WO2003064991A2 WO2003064991A2 (en) 2003-08-07
WO2003064991A3 true WO2003064991A3 (en) 2006-05-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/022681 WO2003064991A2 (en) 2001-07-17 2002-07-17 Method and apparatus using a nonlinear optical technique for detection of probe-target interactions in a field

Country Status (2)

Country Link
AU (1) AU2002365214A1 (en)
WO (1) WO2003064991A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030148391A1 (en) 2002-01-24 2003-08-07 Salafsky Joshua S. Method using a nonlinear optical technique for detection of interactions involving a conformational change
EP2101168A1 (en) * 2008-03-12 2009-09-16 Ecole Normale Superieure De Lyon Surface plasmon resonance enhanced nonlinear microscopy
US9182406B2 (en) 2008-08-04 2015-11-10 Biodesy, Inc. Nonlinear optical detection of molecules comprising an unnatural amino acid possessing a hyperpolarizability
US9428789B2 (en) 2011-03-21 2016-08-30 Biodesy, Inc. Classification of kinase inhibitors using nonlinear optical techniques
US9938560B2 (en) 2012-02-05 2018-04-10 Biodesy, Inc. Methods for identifying modulators of Ras using nonlinear techniques
US9395358B2 (en) 2012-02-05 2016-07-19 Biodesy, Inc. Methods for detecting allosteric modulators of protein
US20130288271A1 (en) 2012-04-25 2013-10-31 Biodesy, Llc Methods for detecting allosteric modulators of protein
CN107075575B (en) * 2014-06-30 2021-12-03 蓝光治疗公司 Systems and methods for high throughput analysis of conformation in biological entities
WO2016106286A1 (en) 2014-12-23 2016-06-30 Biodesy, Inc. Attachment of proteins to interfaces for use in nonlinear optical detection
GB201502194D0 (en) 2015-02-10 2015-03-25 Univ St Andrews Scattered light integrating collector
EP3278087A4 (en) 2015-04-02 2018-12-19 Biodesy, Inc. Methods for determining protein structure using a surface-selective nonlinear optical technique
EP3455628A4 (en) 2016-05-09 2019-12-04 Biodesy, Inc. Methods and devices for detection of peripheral membrane protein interactions using nonlinear optical techniques

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5800992A (en) * 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US6096497A (en) * 1998-06-15 2000-08-01 Biosensor Systems Design, Inc. Electrostatic enzyme biosensor
US6180415B1 (en) * 1997-02-20 2001-01-30 The Regents Of The University Of California Plasmon resonant particles, methods and apparatus
US6455303B1 (en) * 1997-06-04 2002-09-24 Cellectricon Ab Detection of biologically active molecules by use of pre-activated cell-based biosensors in liquid-based separation systems

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5800992A (en) * 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US6180415B1 (en) * 1997-02-20 2001-01-30 The Regents Of The University Of California Plasmon resonant particles, methods and apparatus
US6455303B1 (en) * 1997-06-04 2002-09-24 Cellectricon Ab Detection of biologically active molecules by use of pre-activated cell-based biosensors in liquid-based separation systems
US6096497A (en) * 1998-06-15 2000-08-01 Biosensor Systems Design, Inc. Electrostatic enzyme biosensor

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DELPRINCIPE ET AL: "Two Photo and US laser flash photolysis of Ca cage and dimethoxynitrophenyl-EGTA-4", CELL CALCIUM, vol. 25, 1999, pages 85 *

Also Published As

Publication number Publication date
AU2002365214A1 (en) 2003-09-02
WO2003064991A2 (en) 2003-08-07
AU2002365214A8 (en) 2006-11-02

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