WO2003064596A8 - Desaturase genes, enzymes encoded thereby, and uses thereof - Google Patents

Desaturase genes, enzymes encoded thereby, and uses thereof

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Publication number
WO2003064596A8
WO2003064596A8 PCT/US2003/001698 US0301698W WO03064596A8 WO 2003064596 A8 WO2003064596 A8 WO 2003064596A8 US 0301698 W US0301698 W US 0301698W WO 03064596 A8 WO03064596 A8 WO 03064596A8
Authority
WO
WIPO (PCT)
Prior art keywords
desaturase
isolated polynucleotides
enzymes
enzymes encoded
epa
Prior art date
Application number
PCT/US2003/001698
Other languages
French (fr)
Other versions
WO2003064596A3 (en
WO2003064596A2 (en
Inventor
Pradip Mukerji
Suzette L Pereira
Yung-Sheng Huang
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27658343&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2003064596(A8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Abbott Lab filed Critical Abbott Lab
Priority to DK03710700.0T priority Critical patent/DK1480993T4/en
Priority to JP2003564192A priority patent/JP4780916B2/en
Priority to CA2474894A priority patent/CA2474894C/en
Priority to MXPA04007588A priority patent/MXPA04007588A/en
Priority to EP03710700A priority patent/EP1480993B2/en
Priority to DE60322515T priority patent/DE60322515D1/en
Priority to ES03710700T priority patent/ES2310652T5/en
Publication of WO2003064596A2 publication Critical patent/WO2003064596A2/en
Publication of WO2003064596A3 publication Critical patent/WO2003064596A3/en
Publication of WO2003064596A8 publication Critical patent/WO2003064596A8/en

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

Disclosed are isolated polynucleotides encoding an omega-3 desaturase and a delta-12 desaturase, the enzymes encoded by the isolated polynucleotides, vectors containing the isolated polynucleotides, transgenic hosts that contain the isolated polynucleotides that express the enzymes encoded thereby, methods for producing the desaturase enzymes, and method of using the enzymes to make polyunsaturated fatty acids. The isolated polynucleotides are derived from a fungus, Saprolegnia diclina (ATCC 56851). In particular, omega-3-desaturase may be utilized, for example, in the conversion of arachidonic acid (AA) to eicosapentaenoic acid (EPA). Delta-12 desaturase may be used, for example, in the conversion of oleic acid (OA) to linoleic (LA). EPA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.
PCT/US2003/001698 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded thereby, and uses thereof WO2003064596A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DK03710700.0T DK1480993T4 (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded and applications thereof
JP2003564192A JP4780916B2 (en) 2002-01-30 2003-01-21 Desaturase gene, enzyme encoded by said gene and use thereof
CA2474894A CA2474894C (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded thereby, and uses thereof
MXPA04007588A MXPA04007588A (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded thereby, and uses thereof.
EP03710700A EP1480993B2 (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded thereby, and uses thereof
DE60322515T DE60322515D1 (en) 2002-01-30 2003-01-21 DESATURASEGENE, OF THESE ENCYMES ENCODED AND USES THEREOF
ES03710700T ES2310652T5 (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded by these genes and their use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/060,793 2002-01-30
US10/060,793 US7211656B2 (en) 2002-01-30 2002-01-30 Desaturase genes, enzymes encoded thereby, and uses thereof

Publications (3)

Publication Number Publication Date
WO2003064596A2 WO2003064596A2 (en) 2003-08-07
WO2003064596A3 WO2003064596A3 (en) 2003-12-04
WO2003064596A8 true WO2003064596A8 (en) 2008-07-31

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ID=27658343

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/001698 WO2003064596A2 (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded thereby, and uses thereof

Country Status (11)

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US (3) US7211656B2 (en)
EP (1) EP1480993B2 (en)
JP (1) JP4780916B2 (en)
AT (1) ATE402944T1 (en)
CA (2) CA2474894C (en)
DE (1) DE60322515D1 (en)
DK (1) DK1480993T4 (en)
ES (1) ES2310652T5 (en)
MX (1) MXPA04007588A (en)
PT (1) PT1480993E (en)
WO (1) WO2003064596A2 (en)

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EP1480993B1 (en) 2008-07-30
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US7723503B2 (en) 2010-05-25
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