WO2003064596A3 - Desaturase genes, enzymes encoded thereby, and uses thereof - Google Patents

Desaturase genes, enzymes encoded thereby, and uses thereof Download PDF

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Publication number
WO2003064596A3
WO2003064596A3 PCT/US2003/001698 US0301698W WO03064596A3 WO 2003064596 A3 WO2003064596 A3 WO 2003064596A3 US 0301698 W US0301698 W US 0301698W WO 03064596 A3 WO03064596 A3 WO 03064596A3
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WIPO (PCT)
Prior art keywords
desaturase
isolated polynucleotides
enzymes
enzymes encoded
epa
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PCT/US2003/001698
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French (fr)
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WO2003064596A8 (en
WO2003064596A2 (en
Inventor
Pradip Mukerji
Suzette L Pereira
Yung-Sheng Huang
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Abbott Lab
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Application filed by Abbott Lab filed Critical Abbott Lab
Priority to CA2474894A priority Critical patent/CA2474894C/en
Priority to MXPA04007588A priority patent/MXPA04007588A/en
Priority to ES03710700T priority patent/ES2310652T5/en
Priority to JP2003564192A priority patent/JP4780916B2/en
Priority to DE60322515T priority patent/DE60322515D1/en
Priority to EP03710700A priority patent/EP1480993B2/en
Priority to DK03710700.0T priority patent/DK1480993T4/en
Publication of WO2003064596A2 publication Critical patent/WO2003064596A2/en
Publication of WO2003064596A3 publication Critical patent/WO2003064596A3/en
Publication of WO2003064596A8 publication Critical patent/WO2003064596A8/en

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    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
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    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

Disclosed are isolated polynucleotides encoding an omega-3 desaturase and a delta-12 desaturase, the enzymes encoded by the isolated polynucleotides, vectors containing the isolated polynucleotides, transgenic hosts that contain the isolated polynucleotides that express the enzymes encoded thereby, methods for producing the desaturase enzymes, and method of using the enzymes to make polyunsaturated fatty acids. The isolated polynucleotides are derived from a fungus, Saprolegnia diclina (ATCC 56851). In particular, omega-3-desaturase may be utilized, for example, in the conversion of arachidonic acid (AA) to eicosapentaenoic acid (EPA). Delta-12 desaturase may be used, for example, in the conversion of oleic acid (OA) to linoleic (LA). EPA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.
PCT/US2003/001698 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded thereby, and uses thereof WO2003064596A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA2474894A CA2474894C (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded thereby, and uses thereof
MXPA04007588A MXPA04007588A (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded thereby, and uses thereof.
ES03710700T ES2310652T5 (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded by these genes and their use
JP2003564192A JP4780916B2 (en) 2002-01-30 2003-01-21 Desaturase gene, enzyme encoded by said gene and use thereof
DE60322515T DE60322515D1 (en) 2002-01-30 2003-01-21 DESATURASEGENE, OF THESE ENCYMES ENCODED AND USES THEREOF
EP03710700A EP1480993B2 (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded thereby, and uses thereof
DK03710700.0T DK1480993T4 (en) 2002-01-30 2003-01-21 Desaturase genes, enzymes encoded and applications thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/060,793 US7211656B2 (en) 2002-01-30 2002-01-30 Desaturase genes, enzymes encoded thereby, and uses thereof
US10/060,793 2002-01-30

Publications (3)

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WO2003064596A2 WO2003064596A2 (en) 2003-08-07
WO2003064596A3 true WO2003064596A3 (en) 2003-12-04
WO2003064596A8 WO2003064596A8 (en) 2008-07-31

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US (3) US7211656B2 (en)
EP (1) EP1480993B2 (en)
JP (1) JP4780916B2 (en)
AT (1) ATE402944T1 (en)
CA (2) CA2474894C (en)
DE (1) DE60322515D1 (en)
DK (1) DK1480993T4 (en)
ES (1) ES2310652T5 (en)
MX (1) MXPA04007588A (en)
PT (1) PT1480993E (en)
WO (1) WO2003064596A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8853432B2 (en) 2004-04-22 2014-10-07 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
US9453183B2 (en) 2004-04-22 2016-09-27 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
US9550718B2 (en) 2012-06-15 2017-01-24 Commonwealth Scientific And Industrial Research Organisation Lipid comprising polyunsaturated fatty acids

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7238851B2 (en) * 2001-03-12 2007-07-03 The General Hospital Corporation Non-human transgenic mammals expressing an n-3 desaturase gene
US20040172682A1 (en) * 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US8313911B2 (en) * 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
EP3395945A1 (en) 2003-08-01 2018-10-31 BASF Plant Science GmbH Method for producing polyunsatured fatty acids in transgenic organisms
US11952581B2 (en) 2003-08-01 2024-04-09 Basf Plant Science Gmbh Process for the production of polyunsaturated fatty acids in transgenic organisms
AU2004290052B2 (en) 2003-11-12 2008-12-04 Corteva Agriscience Llc Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous plants and yeast
US7504259B2 (en) * 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
WO2005077022A2 (en) * 2004-02-04 2005-08-25 The General Hospital Corporation Compositions and methods for modifying the content of polyunsaturated fatty acids in biological cells
WO2005083053A2 (en) * 2004-02-27 2005-09-09 Basf Plant Science Gmbh Method for producing unsaturated omega-3 fatty acids in transgenic organisms
ES2421440T3 (en) 2004-02-27 2013-09-02 Basf Plant Science Gmbh Method for preparing polyunsaturated fatty acids in transgenic plants
PT1766023E (en) * 2004-06-04 2010-12-09 Fluxome Sciences As Metabolically engineered cells for the production of polyunsaturated fatty acids
JP4587451B2 (en) * 2004-08-20 2010-11-24 サントリーホールディングス株式会社 Polypeptide having ω3 fatty acid desaturation activity, polynucleotide encoding the polypeptide, and use thereof
US7588931B2 (en) * 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
AR059376A1 (en) 2006-02-21 2008-03-26 Basf Plant Science Gmbh PROCEDURE FOR THE PRODUCTION OF POLYINSATURATED FATTY ACIDS
US7943823B2 (en) 2006-04-28 2011-05-17 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US20070254299A1 (en) * 2006-05-01 2007-11-01 Yadav Narendra S Fungal delta 12 desaturase and delta 15 desaturase motifs
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
AU2013202498B2 (en) * 2006-08-24 2015-04-30 Basf Plant Science Gmbh Isolation and characterization of a novel Pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains
US8816106B2 (en) 2006-08-29 2014-08-26 Commonwealth Scientific And Industrial Research Organisation Synthesis of fatty acids
EP2121934A2 (en) * 2007-02-12 2009-11-25 E. I. Du Pont de Nemours and Company Production of arachidonic acid in oilseed plants
US7943365B2 (en) 2007-05-03 2011-05-17 E.I. Du Pont De Nemours And Company Δ-5 desaturases and their use in making polyunsaturated fatty acids
DE102007058991A1 (en) 2007-12-07 2009-06-25 Bayer Materialscience Ag The use of low-chloride, low-carbonate silica sand as retention material for solid acid catalysts, especially in solid-bed reactors for condensation reactions, preferably for production of Bisphenol A
IT1390848B1 (en) * 2008-07-31 2011-10-19 Neuroscienze Pharmaness S C A R L PHARMACEUTICAL COMPOUNDS
EP2149370A1 (en) 2008-07-31 2010-02-03 Neuroscienze Pharmaness S.C. A R.L. Diazabicyclic compounds and microemulsions thereof
AR074364A1 (en) 2008-11-18 2011-01-12 Commw Scient Ind Res Org ENZYMES AND METHOD TO PRODUCE OMEGA FATTY ACIDS -3
AU2012226528B2 (en) 2011-03-07 2015-08-20 Dsm Nutritional Products Ag Engineering thraustochytrid microorganisms
WO2013153404A1 (en) 2012-04-12 2013-10-17 Rothamsted Research Ltd Production of omega-3 long chain polyunsaturated fatty acids
GB201217524D0 (en) 2012-10-01 2012-11-14 Rothamsted Res Ltd Recombinant organisms
PT2970926T (en) 2013-03-13 2018-03-22 Dsm Nutritional Products Ag Engineering microorganisms
EP3040415A4 (en) 2013-08-27 2017-04-12 Kyoto University Omega-3 UNSATURATED FATTY ACID ENZYME AND METHOD FOR PRODUCING EICOSAPENTAENOIC ACID
WO2015089587A1 (en) 2013-12-18 2015-06-25 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
CN105219789B (en) 2014-06-27 2023-04-07 联邦科学技术研究组织 Extracted plant lipids comprising docosapentaenoic acid
CN107109378B (en) 2014-12-25 2021-12-07 国立大学法人京都大学 Novel omega-3 fatty acid desaturase and method for producing eicosapentaenoic acid

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658767A (en) * 1991-01-24 1997-08-19 Martek Corporation Arachidonic acid and methods for the production and use thereof
US6150144A (en) * 1996-08-23 2000-11-21 Suntory Limited Process for producing omega-9 highly unsaturated fatty acid and lipid containing the same

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57501692A (en) 1980-09-24 1982-09-16
DE3381518D1 (en) 1982-01-22 1990-06-07 Cetus Corp METHOD FOR CHARACTERIZING HLA AND THE CDNS TEST AGENTS USED IN IT.
US4582788A (en) 1982-01-22 1986-04-15 Cetus Corporation HLA typing method and cDNA probes used therein
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4943674A (en) * 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US5420034A (en) 1986-07-31 1995-05-30 Calgene, Inc. Seed-specific transcriptional regulation
GB8507058D0 (en) 1985-03-19 1985-04-24 Efamol Ltd Pharmaceutical & dietary compositions
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
GB8524276D0 (en) 1985-10-02 1985-11-06 Efamol Ltd Pharmaceutical & dietary compositions
GB8524275D0 (en) 1985-10-02 1985-11-06 Efamol Ltd Pharmaceutical & dietary compositions
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US5107065A (en) * 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
CA1338683C (en) 1988-09-13 1996-10-29 Efamol Holdings Plc Fatty acid therapy and compositions
US5231020A (en) * 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5106739A (en) 1989-04-18 1992-04-21 Calgene, Inc. CaMv 355 enhanced mannopine synthase promoter and method for using same
US5175095A (en) 1989-07-19 1992-12-29 Calgene, Inc. Ovary-tissue transcriptional factors
ATE110778T1 (en) 1990-05-25 1994-09-15 Du Pont NUCLEOTIDE SEQUENCE OF THE GENE FOR SOYA STEAROYL-ACP DESATURASE.
DE69033623T2 (en) * 1990-10-22 2001-05-23 Aicello Chemical Co ENGRAVING PROCESS WITH IMAGE MASK AND LIGHT-SENSITIVE LAMINATE FILM FOR THIS IMAGE MASK
US5518908A (en) 1991-09-23 1996-05-21 Monsanto Company Method of controlling insects
PH31293A (en) 1991-10-10 1998-07-06 Rhone Poulenc Agrochimie Production of y-linolenic acid by a delta6-desaturage.
US5614393A (en) * 1991-10-10 1997-03-25 Rhone-Poulenc Agrochimie Production of γ-linolenic acid by a Δ6-desaturase
EP0616644B1 (en) 1991-12-04 2003-07-02 E.I. Du Pont De Nemours And Company Fatty acid desaturase genes from plants
US5912120A (en) 1992-04-09 1999-06-15 The United States Of America As Represented By The Department Of Health And Human Services, Cloning, expression and diagnosis of human cytochrome P450 2C19: the principal determinant of s-mephenytoin metabolism
DK0668919T3 (en) 1992-11-17 2003-09-15 Du Pont Genes for microsomal delta-12 fatty acid desaturases and related plant enzymes
EP0686197B1 (en) 1993-02-26 2000-06-07 Calgene LLC Geminivirus-based gene expression system
DE4339010C2 (en) * 1993-06-25 2000-05-18 Pt Sub Inc Photohardenable product for printing plates
CA2184688A1 (en) * 1994-03-09 1995-09-14 Pedro Antonio Prieto Transgenic animals producing oligosaccharides and glycoconjugates
NZ279676A (en) 1994-03-09 1998-04-27 Abbott Lab Humanized milk produced by non-human transgenic mammal transformed with a heterologous gene coding for human enzyme producing human oligosaccharides and glycoconjugates
US5750176A (en) * 1994-03-09 1998-05-12 Abbott Laboratories Transgenic non-human mammal milk comprising 2'-fucosyl-lactose
US5922506A (en) * 1994-10-20 1999-07-13 Agfa-Gevaert, N.V. Imaging element comprising a hydrophobic photopolymerizable composition and method for producing lithographic plates therewith
US5631152A (en) 1994-10-26 1997-05-20 Monsanto Company Rapid and efficient regeneration of transgenic plants
US5654402A (en) 1994-10-26 1997-08-05 Michigan State University Methods and compositions relating to plant Δ6 palmitoyl-acyl carrier protein desaturase
US5677108A (en) * 1995-04-28 1997-10-14 Polaroid Corporation On-press removable quenching overcoat for lithographic plates
US6143470A (en) * 1995-06-23 2000-11-07 Nguyen; My T. Digital laser imagable lithographic printing plates
US5820932A (en) * 1995-11-30 1998-10-13 Sun Chemical Corporation Process for the production of lithographic printing plates
US6140005A (en) * 1996-04-23 2000-10-31 Agfa-Gevaert, N.V. Imaging element and a method for producing a lithographic plate therewith
US6106992A (en) * 1996-11-14 2000-08-22 Dai Nippon Printing Co., Ltd. Photoresist film and process for producing back plate of plasma display panel
US6194167B1 (en) 1997-02-18 2001-02-27 Washington State University Research Foundation ω-3 fatty acid desaturase
US5968809A (en) † 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
TR199902474T2 (en) † 1997-04-11 2000-02-21 Calgene Llc Methods and compositions for the synthesis of long chain polyunsaturated fatty acids in plants.
US7008664B1 (en) * 1998-06-11 2006-03-07 E. I. Du Pont De Nemours And Company Method for improving the carcass quality of an animal
US6403349B1 (en) * 1998-09-02 2002-06-11 Abbott Laboratories Elongase gene and uses thereof
DE19941609A1 (en) * 1999-09-01 2001-03-08 Inst Pflanzenbiochemie Ipb Fatty acid desaturase gene from plants
JP2001169731A (en) * 1999-12-17 2001-06-26 Showa Denko Kk Lipid metabolism improving agent for animal
GB0016452D0 (en) * 2000-07-04 2000-08-23 Kilgowan Limited Vitamin K and essential fatty acids
US6849383B2 (en) * 2000-07-26 2005-02-01 Toyo Boseki Kabushiki Kaisha Photosensitive resin laminate, and signboard plate and signboard made of the laminate
TWI324181B (en) † 2001-04-16 2010-05-01 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658767A (en) * 1991-01-24 1997-08-19 Martek Corporation Arachidonic acid and methods for the production and use thereof
US6150144A (en) * 1996-08-23 2000-11-21 Suntory Limited Process for producing omega-9 highly unsaturated fatty acid and lipid containing the same

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8853432B2 (en) 2004-04-22 2014-10-07 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
US9453183B2 (en) 2004-04-22 2016-09-27 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
US9458410B2 (en) 2004-04-22 2016-10-04 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
US9550718B2 (en) 2012-06-15 2017-01-24 Commonwealth Scientific And Industrial Research Organisation Lipid comprising polyunsaturated fatty acids
US9556102B2 (en) 2012-06-15 2017-01-31 Commonwealth Scientific And Industrial Research Organisation Process for producing ethyl esters of polyunsaturated fatty acids

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