WO2003064590A3 - Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate - Google Patents

Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate Download PDF

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Publication number
WO2003064590A3
WO2003064590A3 PCT/US2002/033295 US0233295W WO03064590A3 WO 2003064590 A3 WO2003064590 A3 WO 2003064590A3 US 0233295 W US0233295 W US 0233295W WO 03064590 A3 WO03064590 A3 WO 03064590A3
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WO
WIPO (PCT)
Prior art keywords
analysis
methods
microfluidic systems
transport
lysis
Prior art date
Application number
PCT/US2002/033295
Other languages
French (fr)
Other versions
WO2003064590A2 (en
Inventor
Christopher T Culbertson
Stephen C Jacobson
Maxine A Mcclain
J Michael Ramsey
Original Assignee
Ut Battelle Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ut Battelle Llc filed Critical Ut Battelle Llc
Priority to AU2002365220A priority Critical patent/AU2002365220A1/en
Publication of WO2003064590A2 publication Critical patent/WO2003064590A2/en
Publication of WO2003064590A3 publication Critical patent/WO2003064590A3/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Abstract

Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods (Figure 5A), which employ an electric field to rupture the cell membrane (Figure 5B), cause unusually rapid lysis (Figure 5C), thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes (Figure 5D).
PCT/US2002/033295 2001-10-19 2002-10-18 Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate WO2003064590A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002365220A AU2002365220A1 (en) 2001-10-19 2002-10-18 Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/041,821 2001-10-19
US10/041,821 US6783647B2 (en) 2001-10-19 2001-10-19 Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate

Publications (2)

Publication Number Publication Date
WO2003064590A2 WO2003064590A2 (en) 2003-08-07
WO2003064590A3 true WO2003064590A3 (en) 2003-12-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/033295 WO2003064590A2 (en) 2001-10-19 2002-10-18 Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate

Country Status (3)

Country Link
US (2) US6783647B2 (en)
AU (1) AU2002365220A1 (en)
WO (1) WO2003064590A2 (en)

Families Citing this family (144)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6868289B2 (en) * 2002-10-02 2005-03-15 Standen Ltd. Apparatus for treating a tumor or the like and articles incorporating the apparatus for treatment of the tumor
US6913697B2 (en) 2001-02-14 2005-07-05 Science & Technology Corporation @ Unm Nanostructured separation and analysis devices for biological membranes
US6783647B2 (en) * 2001-10-19 2004-08-31 Ut-Battelle, Llc Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate
US6815209B2 (en) * 2001-11-16 2004-11-09 Cornell Research Foundation, Inc. Laser-induced cell lysis system
AU2003216175A1 (en) * 2002-02-04 2003-09-02 Colorado School Of Mines Laminar flow-based separations of colloidal and cellular particles
CA2477702A1 (en) * 2002-03-05 2003-09-18 Caliper Life Sciences, Inc. Mixed mode microfluidic systems abstract of the disclosure
EP1517752A4 (en) * 2002-05-03 2009-07-08 Univ California Fast electrical lysis of cells and rapid collection of the contents thereof using capillary electrophoresis
US7901939B2 (en) * 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
JP4855680B2 (en) 2002-05-09 2012-01-18 ザ・ユニバーシティ・オブ・シカゴ Apparatus and method for transport and reaction by pressure driven plug
ES2375724T3 (en) 2002-09-27 2012-03-05 The General Hospital Corporation MICROFLUDE DEVICE FOR SEPERATION OF CELLS AND ITS USES.
US20040091850A1 (en) * 2002-11-08 2004-05-13 Travis Boone Single cell analysis of membrane molecules
DE10311716A1 (en) 2003-03-17 2004-10-14 Evotec Oai Ag Method and device for separating particles in a liquid flow
JP4356694B2 (en) * 2003-03-19 2009-11-04 日本電気株式会社 Microchip and sample extraction method, sample separation method, sample analysis method, and sample recovery method
US7592139B2 (en) 2004-09-24 2009-09-22 Sandia National Laboratories High temperature flow-through device for rapid solubilization and analysis
US7608446B2 (en) * 2004-09-30 2009-10-27 Alcatel-Lucent Usa Inc. Nanostructured surface for microparticle analysis and manipulation
DE102004055662A1 (en) * 2004-11-18 2006-06-01 Evotec Technologies Gmbh Microfluidic system with channel widening
US7488596B2 (en) * 2004-12-17 2009-02-10 Samsung Electronics Co., Ltd. Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same
US20060171846A1 (en) * 2005-01-10 2006-08-03 Marr David W M Microfluidic systems incorporating integrated optical waveguides
US7727363B2 (en) * 2005-02-02 2010-06-01 Ut-Battelle, Llc Microfluidic device and methods for focusing fluid streams using electroosmotically induced pressures
US9097723B2 (en) * 2005-04-01 2015-08-04 Caliper Life Sciences, Inc. Method and apparatus for performing peptide digestion on a microfluidic device
WO2006108087A2 (en) * 2005-04-05 2006-10-12 Cellpoint Diagnostics Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070196820A1 (en) * 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8158377B2 (en) 2005-08-31 2012-04-17 Ut-Battelle, Llc Biosensor method and system based on feature vector extraction
US8198075B2 (en) * 2005-08-31 2012-06-12 Ut-Battelle, Llc Method and apparatus for enhanced detection of toxic agents
EP1764418B1 (en) * 2005-09-14 2012-08-22 STMicroelectronics Srl Method and device for the treatment of biological samples using dielectrophoresis
KR100670590B1 (en) 2005-10-05 2007-01-17 주식회사 디지탈바이오테크놀러지 Microchip with expansion channel and flowcytometer using this microchip
US20070105206A1 (en) * 2005-10-19 2007-05-10 Chang Lu Fluidic device
CN101365781A (en) * 2005-11-21 2009-02-11 阿普尔拉公司 Portable preparation, analysis, and detection apparatus for nucleic acid processing
ATE466537T1 (en) * 2006-01-03 2010-05-15 Alcon Inc SYSTEM FOR CLIVING AND REMOVAL OF PROTEINOUS TISSUE
US9878326B2 (en) * 2007-09-26 2018-01-30 Colorado School Of Mines Fiber-focused diode-bar optical trapping for microfluidic manipulation
US9487812B2 (en) 2012-02-17 2016-11-08 Colorado School Of Mines Optical alignment deformation spectroscopy
US8119976B2 (en) * 2007-07-03 2012-02-21 Colorado School Of Mines Optical-based cell deformability
US9885644B2 (en) 2006-01-10 2018-02-06 Colorado School Of Mines Dynamic viscoelasticity as a rapid single-cell biomarker
KR100723424B1 (en) * 2006-04-07 2007-05-30 삼성전자주식회사 Microfluidic device and method for concentrate and lyse cells or viruses, and method for manufacturing the microfluidic device
ITTO20060273A1 (en) * 2006-04-12 2007-10-13 Silicon Biosystem S P A METHODS AND EQUIPMENT FOR THE SELECTION AND / OR PROCESSING OF PARTICLES, IN PARTICULAR FOR THE SELECTIVE AND / OR OPTIMIZED CELL LYSIS
ITTO20060278A1 (en) 2006-04-13 2007-10-14 Silicon Biosystem S P A METHOD FOR THE SELECTION AND / OR PROCESSING OF PARTICLES, IN PARTICULAR CELLS
US9862984B2 (en) 2006-04-21 2018-01-09 Nanobiosym, Inc. Single-molecule platform for drug discovery: methods and apparatuses for drug discovery, including discovery of anticancer and antiviral agents
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2589668A1 (en) 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
US8691164B2 (en) * 2007-04-20 2014-04-08 Celula, Inc. Cell sorting system and methods
WO2009015390A2 (en) * 2007-07-26 2009-01-29 University Of Chicago Co-incuating confined microbial communities
EP2178641B1 (en) * 2007-08-09 2018-04-11 Progenity, Inc. Methods and devices for correlated, multi-parameter single cell measurements and recovery of remnant biological material
WO2009028093A1 (en) * 2007-08-31 2009-03-05 Shimadzu Corporation Flow cell, apparatus for concentrating radioactive fluorine anion, and method of concentrating radioactive fluorine anion
WO2009029613A1 (en) * 2007-08-31 2009-03-05 The Board Of Regents, The University Of Texas System Apparatus for performing magnetic electroporation
WO2009032827A2 (en) * 2007-09-04 2009-03-12 Purdue Research Foundation Electroporative flow cytometry
US20090062828A1 (en) * 2007-09-04 2009-03-05 Colorado School Of Mines Magnetic field-based colloidal atherectomy
US10722250B2 (en) 2007-09-04 2020-07-28 Colorado School Of Mines Magnetic-field driven colloidal microbots, methods for forming and using the same
FR2921157A1 (en) * 2007-09-18 2009-03-20 Saint Gobain ELECTRODE FOR MICROFLUIDIC SYSTEM
US8304245B2 (en) * 2007-11-02 2012-11-06 Children's Hospital And Research Center At Oakland Microfluidic flow lysometer device, system and method
US20090250347A1 (en) * 2008-04-03 2009-10-08 Protea Biosciences, Inc. Microfluidic devices & processes for electrokinetic transport
US20090250345A1 (en) * 2008-04-03 2009-10-08 Protea Biosciences, Inc. Microfluidic electroelution devices & processes
US20110095225A1 (en) * 2009-04-20 2011-04-28 Origin Oil, Inc. Systems, apparatuses, and methods for extracting non-polar lipids from an aqueous algae slurry and lipids produced therefrom
AU2010257118B2 (en) 2009-06-04 2014-08-28 Lockheed Martin Corporation Multiple-sample microfluidic chip for DNA analysis
CA2769320C (en) * 2009-08-02 2021-01-26 Qvella Corporation Cell concentration, capture and lysis devices and methods of use thereof
US8834807B2 (en) * 2009-08-12 2014-09-16 Caliper Life Sciences, Inc. Device and method for improving sample injection and stacking
US20110065161A1 (en) * 2009-09-14 2011-03-17 Board Of Regents, The University Of Texas System Bipolar solid state marx generator
US20110118729A1 (en) * 2009-11-13 2011-05-19 Alcon Research, Ltd High-intensity pulsed electric field vitrectomy apparatus with load detection
US20110135626A1 (en) * 2009-12-08 2011-06-09 Alcon Research, Ltd. Localized Chemical Lysis of Ocular Tissue
US20110144562A1 (en) * 2009-12-14 2011-06-16 Alcon Research, Ltd. Localized Pharmacological Treatment of Ocular Tissue Using High-Intensity Pulsed Electrical Fields
WO2011081897A1 (en) * 2009-12-15 2011-07-07 Alcon Research, Ltd. High-intensity pulsed electric field vitrectomy apparatus
WO2012009320A2 (en) * 2010-07-15 2012-01-19 Advanced Liquid Logic, Inc. Systems for and methods of promoting cell lysis in droplet actuators
US20120021481A1 (en) * 2010-07-20 2012-01-26 Board Of Regents, The University Of Texas System Electromechanical lysing of algae cells
US8546979B2 (en) 2010-08-11 2013-10-01 Alcon Research, Ltd. Self-matching pulse generator with adjustable pulse width and pulse frequency
MX2013004184A (en) 2010-10-15 2013-07-29 Lockheed Corp Micro fluidic optic design.
ES2759824T3 (en) 2011-07-25 2020-05-12 Qvella Corp Methods and devices for electrical sample preparation
US11053535B2 (en) * 2011-09-12 2021-07-06 The University Of North Carolina At Chapel Hill Devices with a fluid transport nanochannel intersected by a fluid sensing nanochannel and related methods
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
US9304065B2 (en) 2012-02-29 2016-04-05 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
CA2889415C (en) 2012-10-24 2020-06-02 Genmark Diagnostics, Inc. Integrated multiplex target analysis
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
TWI499552B (en) * 2012-12-07 2015-09-11 Univ Nat Cheng Kung Droplet-generating method and device
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3862435A1 (en) 2013-02-08 2021-08-11 10X Genomics, Inc. Polynucleotide barcode generation
AU2014228937A1 (en) 2013-03-15 2015-10-15 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
EP2969217A2 (en) 2013-03-15 2016-01-20 Genmark Diagnostics Inc. Systems, methods, and apparatus for manipulating deformable fluid vessels
US10954546B2 (en) 2013-09-24 2021-03-23 Ait Austrian Institute Of Technology Gmbh Method for pathogen isolation
CA2866542A1 (en) * 2013-10-08 2015-04-08 Ponnambalam Selvaganapathy Integrated reagentless sample preprocessing for molecular diagnostics using a nanoporous membrane based microfluidic device
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015103331A1 (en) * 2013-12-31 2015-07-09 Canon U.S. Life Sciences, Inc. Methods and systems for continuous flow cell lysis in a microfluidic device
US10117563B2 (en) 2014-01-09 2018-11-06 Gyrus Acmi, Inc. Polyp detection from an image
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
CN106795553B (en) 2014-06-26 2021-06-04 10X基因组学有限公司 Methods of analyzing nucleic acids from individual cells or cell populations
CA2964472A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
EP3244992B1 (en) 2015-01-12 2023-03-08 10X Genomics, Inc. Processes for barcoding nucleic acids
BR112017018054A2 (en) 2015-02-24 2018-07-24 10X Genomics Inc Methods for Covering Targeted Nucleic Acid Sequences
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
CA3019814A1 (en) 2016-04-04 2017-10-12 CyteQuest, Inc. System, device and method for electroporation of cells
WO2017184178A1 (en) * 2016-04-22 2017-10-26 Hewlett-Packard Development Company, L.P. Cell lysis
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
EP3700672B1 (en) 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
WO2020032969A1 (en) * 2018-08-10 2020-02-13 Hewlett-Packard Development Company, L.P. Conductivity-based lysis monitors
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US20220040697A1 (en) * 2019-02-01 2022-02-10 Hewlett-Packard Development Company, L.P. Cell analysis systems
US20220056397A1 (en) * 2019-02-01 2022-02-24 Hewlett-Packard Development Company, L.P. Cell analysis systems
WO2020159545A1 (en) * 2019-02-01 2020-08-06 Hewlett-Packard Development Company, L.P. Chemical lysis systems
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
US20230011683A1 (en) * 2020-01-23 2023-01-12 Hewlett-Packard Development Company, L.P. Thermal cell lysis chamber with lysis control circuitry
WO2021221658A1 (en) * 2020-04-30 2021-11-04 Hewlett-Packard Development Company, L.P. Reagent injections into cells
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN112011446A (en) * 2020-08-31 2020-12-01 吉林大学 Micro-fluidic chip for bacterial lysis, bacterial lysis device and application thereof
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999025816A1 (en) * 1997-11-14 1999-05-27 California Institute Of Technology Cell lysis device
WO1999038612A1 (en) * 1998-01-30 1999-08-05 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
GB2355717A (en) * 1999-10-28 2001-05-02 Amersham Pharm Biotech Uk Ltd DNA isolation method
US6322683B1 (en) * 1999-04-14 2001-11-27 Caliper Technologies Corp. Alignment of multicomponent microfabricated structures
US6544734B1 (en) * 1998-10-09 2003-04-08 Cynthia G. Briscoe Multilayered microfluidic DNA analysis system and method

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4676274A (en) 1985-02-28 1987-06-30 Brown James F Capillary flow control
US5304487A (en) 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US6120666A (en) 1996-09-26 2000-09-19 Ut-Battelle, Llc Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same
US5858187A (en) 1996-09-26 1999-01-12 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing electrodynamic focusing on a microchip
US6110343A (en) 1996-10-04 2000-08-29 Lockheed Martin Energy Research Corporation Material transport method and apparatus
EP1003847A4 (en) 1997-09-04 2005-04-06 Science Res Lab Inc Cell separation using electric fields
US6787339B1 (en) * 2000-10-02 2004-09-07 Motorola, Inc. Microfluidic devices having embedded metal conductors and methods of fabricating said devices
US6783647B2 (en) * 2001-10-19 2004-08-31 Ut-Battelle, Llc Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999025816A1 (en) * 1997-11-14 1999-05-27 California Institute Of Technology Cell lysis device
WO1999038612A1 (en) * 1998-01-30 1999-08-05 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US6544734B1 (en) * 1998-10-09 2003-04-08 Cynthia G. Briscoe Multilayered microfluidic DNA analysis system and method
US6572830B1 (en) * 1998-10-09 2003-06-03 Motorola, Inc. Integrated multilayered microfludic devices and methods for making the same
US6322683B1 (en) * 1999-04-14 2001-11-27 Caliper Technologies Corp. Alignment of multicomponent microfabricated structures
GB2355717A (en) * 1999-10-28 2001-05-02 Amersham Pharm Biotech Uk Ltd DNA isolation method

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US7419575B2 (en) 2008-09-02
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