WO2003062270B1 - Fluorescent protein from aequorea coerulscens and uses therof - Google Patents

Fluorescent protein from aequorea coerulscens and uses therof

Info

Publication number
WO2003062270B1
WO2003062270B1 PCT/IB2003/000907 IB0300907W WO03062270B1 WO 2003062270 B1 WO2003062270 B1 WO 2003062270B1 IB 0300907 W IB0300907 W IB 0300907W WO 03062270 B1 WO03062270 B1 WO 03062270B1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
peptide
cell
detecting
labeling
Prior art date
Application number
PCT/IB2003/000907
Other languages
French (fr)
Other versions
WO2003062270A2 (en
WO2003062270A8 (en
WO2003062270A3 (en
Inventor
Nadejda Gurskaya
Arkadiy Fradlov
Sergey Lukyanov
Natalia Punkova
Original Assignee
Nadejda Gurskaya
Arkadiy Fradlov
Sergey Lukyanov
Natalia Punkova
Evrogen Jsc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CA2474108A priority Critical patent/CA2474108C/en
Priority to IL16256803A priority patent/IL162568A0/en
Priority to DK03706812.9T priority patent/DK1485481T3/en
Application filed by Nadejda Gurskaya, Arkadiy Fradlov, Sergey Lukyanov, Natalia Punkova, Evrogen Jsc filed Critical Nadejda Gurskaya
Priority to EP03706812A priority patent/EP1485481B1/en
Priority to US10/501,629 priority patent/US7432053B2/en
Priority to AU2003208520A priority patent/AU2003208520B2/en
Priority to AT03706812T priority patent/ATE458816T1/en
Priority to DE60331422T priority patent/DE60331422D1/en
Priority to JP2003562147A priority patent/JP4510464B2/en
Publication of WO2003062270A2 publication Critical patent/WO2003062270A2/en
Publication of WO2003062270A3 publication Critical patent/WO2003062270A3/en
Publication of WO2003062270B1 publication Critical patent/WO2003062270B1/en
Priority to IL162568A priority patent/IL162568A/en
Publication of WO2003062270A8 publication Critical patent/WO2003062270A8/en
Priority to US12/200,113 priority patent/US7879988B2/en
Priority to US12/199,983 priority patent/US7667016B2/en
Priority to US12/200,064 priority patent/US7897726B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

Abstract

The present invention provides nucleic acid compositions encoding a novel colorless GFP-like protein, acGFP, from Aequorea coerulscens and fluorescent and non-fluorescent mutants and derivatives thereof, as well as peptides and proteins encoded by these nucleic acid compositions. The subject protein and nucleic acid compositions of the present invention are colored and/or fluorescent and/or can be photoactivated, and can be used in a variety of different biological applications, particularly for labeling. Finally, kits for use in such biological applications are provided.

Claims

AMENDED CLAIMS[received by the International Bureau on 17 November 2003 (17.11.03); original claims 1-20 replaced by new claims 1-32 (3 pages)]
1. An isolated nucleic acid which encodes an Aequorea coerulescens peptide, selected from:
(a) a nucleic acid which encodes a protein comprising an amino acid sequence SEQ ID NO: 02;
(b) a nucleic acid comprising a nucleotide sequence SEQ ID NO: 01.
2. An isolated nucleic acid selected from the group consisting of:
(a) a nucleic acid derived from the nucleic acid of claim 1 using at least one of site-directed mutagenesis and/or random mutagenesis;
(b) a nucleic acid coding for an amino acid sequence selected from SEQ ID NOs: 04, 06, 08, 10, 12, 14, 16, 18, 20, 22, or 24; or
(c) a nucleic acid comprising a nucleotide sequence selected from SEQ ID NOs: 03, 05, 07, 09, 11 , 13, 15, 17, 19, 21 , or 23.
(d) a nucleic acid differing from the nucleic acid of (a) above due to degeneracy of the genetic code
3. An isolated nucleic acid of claim 2, wherein said nucleic acid encodes a fluorescent protein
4. An expression cassette comprising
(a) the nucleic acid of claim 2; and
(b) regulatory elements necessary for expression of the nucleic acid in the cell.
5. An expression cassette comprising
(a) the nucleic acid of claim 3; and
(b) regulatory elements necessary for expression of the nucleic acid in the cell.
6. A cell, or progeny thereof, comprising the expression cassette of
52 AMENDED CLAIMS claim 4.
7. A cell, or progeny thereof, comprising the expression cassette of claim 5.
8. An isolated peptide encoded by the nucleic acid of claim 2.
9. An antibody binding specifically to the peptide of claim 8.
10. A fusion peptide incorporating the peptide of claim 8.
11. A transgenic organism capable of expressing the peptide of claim 8.
12. A method for labeling or detecting a biological molecule comprising coupling said biological molecule to the peptide of claim 8.
13. A method for labeling or detecting a cell or cell organelle comprising production of the peptide of claim 8 in the cell.
14. A method for detecting a gene expression comprising production of the peptide of claim 8 in the cell.
15. An isolated peptide encoded by the nucleic acid of claim 3.
16. An antibody binding specifically to the peptide of claim 15.
17. A fusion peptide incorporating the peptide of claim 15.
18. A transgenic organism capable of expressing the peptide of claim 15.
19. A method for labeling or detecting a biological molecule comprising coupling said biological molecule to the peptide of claim 15.
20. A method for labeling or detecting a cell or cell organelle comprising production of the peptide of claim 15 in the cell.
21. A method for detecting a gene expression comprising production of the peptide of claim 15 in the cell.
22. A nucleic acid fragment, wherein said fragment is selected from the group consisting of:
53 AMENDED CLAIMS
(a) a fragment encoding a peptide of at least 100 amino acid residues in length from SEQ ID NOs: 02, 04, 06, 08, 10, 12, 14, 16, 18, 20, 22, or 24; or
(b) a fragment substantially similar or identical to a nucleotide sequence of at least 300 residues in length from SEQ ID NO: 01 , 03, 05, 07, 09, 11 , 13, 15, 17, 19, 21 , or 23.
23. A nucleic acid molecule comprising a nucleic acid fragment of claim 22, wherein the nucleic acid encodes a fluorescent protein
24. An expression cassette comprising
(a) the nucleic acid molecule of claim 23; and
(b) regulatory elements necessary for expression of the nucleic acid fragment in the cell.
25. A cell, or progeny thereof, comprising the expression cassette of claim 24.
26. An isolated peptide encoded by the nucleic acid fragment of claim 23.
27. An antibody binding specifically to the peptide of claim 26.
28. A fusion peptide incorporating the peptide of claim 26.
29. A transgenic organism capable of expressing the peptide of claim 26.
30. A method for labeling or detecting a biological molecule comprising coupling said biological molecule to the peptide claim 26.
31. A method for labeling or detecting a cell or cell organelle comprising production of the peptide of claim 26 in the cell.
32. A method for detecting a gene expression comprising production of the peptide of claim 26 in a cell.
54
PCT/IB2003/000907 2002-01-22 2003-01-17 Fluorescent protein from aequorea coerulscens and uses therof WO2003062270A2 (en)

Priority Applications (13)

Application Number Priority Date Filing Date Title
JP2003562147A JP4510464B2 (en) 2002-01-22 2003-01-17 Novel fluorescent protein derived from Aequorea coerulescens and method of use thereof
IL16256803A IL162568A0 (en) 2002-01-22 2003-01-17 fluorescent protein from aequorea coerulscens and uses thereof
DE60331422T DE60331422D1 (en) 2002-01-22 2003-01-17 FLUORESCENT PROTEIN FROM AEQUOREA COERULESCENS AND ITS USES
EP03706812A EP1485481B1 (en) 2002-01-22 2003-01-17 Fluorescent protein from aequorea coerulescens and uses thereof
US10/501,629 US7432053B2 (en) 2002-01-22 2003-01-17 Fluorescent protein from Aequorea coerulscens and methods for using the same
AU2003208520A AU2003208520B2 (en) 2002-01-22 2003-01-17 Fluorescent protein from aequorea coerulscens and uses therof
AT03706812T ATE458816T1 (en) 2002-01-22 2003-01-17 FLUORESCENT PROTEIN FROM AEQUOREA COERULESCENS AND THEIR USES
CA2474108A CA2474108C (en) 2002-01-22 2003-01-17 Novel fluorescent proteins from aequorea coerulscens and methods for using same
DK03706812.9T DK1485481T3 (en) 2002-01-22 2003-01-17 Fluorescent protein from Aequorea Coerulescens and uses thereof
IL162568A IL162568A (en) 2002-01-22 2004-06-16 Isolated nucleic acid encoding a fluorescent mutant of an aequorea coerulescens protein
US12/200,113 US7879988B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same
US12/199,983 US7667016B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same
US12/200,064 US7897726B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35151802P 2002-01-22 2002-01-22
US60/351,518 2002-01-22

Related Child Applications (4)

Application Number Title Priority Date Filing Date
US10501629 A-371-Of-International 2003-01-17
US12/200,064 Division US7897726B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same
US12/200,113 Division US7879988B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same
US12/199,983 Division US7667016B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same

Publications (4)

Publication Number Publication Date
WO2003062270A2 WO2003062270A2 (en) 2003-07-31
WO2003062270A3 WO2003062270A3 (en) 2003-11-27
WO2003062270B1 true WO2003062270B1 (en) 2004-04-01
WO2003062270A8 WO2003062270A8 (en) 2004-11-04

Family

ID=27613506

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2003/000907 WO2003062270A2 (en) 2002-01-22 2003-01-17 Fluorescent protein from aequorea coerulscens and uses therof

Country Status (12)

Country Link
US (4) US7432053B2 (en)
EP (1) EP1485481B1 (en)
JP (3) JP4510464B2 (en)
AT (1) ATE458816T1 (en)
AU (1) AU2003208520B2 (en)
CA (1) CA2474108C (en)
DE (1) DE60331422D1 (en)
DK (1) DK1485481T3 (en)
ES (1) ES2340154T3 (en)
IL (2) IL162568A0 (en)
RU (1) RU2330886C2 (en)
WO (1) WO2003062270A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2340154T3 (en) * 2002-01-22 2010-05-31 Evrogen Ip FLUORESCENT PROTEINS OF AEQUOREA COERULESCENS AND USE OF THE SAME.
EP1576157B1 (en) 2002-12-26 2010-07-21 Zakrytoe Aktsionernoe Obschestvo " Evrogen" Fluorescent proteins from copepoda species and methods for using same
AU2005311680A1 (en) 2004-12-01 2006-06-08 Proteologics, Inc. Ubiquitin ligase assays and related reagents
UA93922C2 (en) * 2009-03-20 2011-03-25 Михаил Викторович Разуменко Process for production of peptides, specifically recognizing the cells of particular type, meant for therapeutic goals
US20110097329A1 (en) 2009-06-26 2011-04-28 Massachusetts Institute Of Technology Compositions and methods for treating cancer and modulating stress granule formation
US8435961B2 (en) 2009-06-26 2013-05-07 Massachusetts Institute Of Technology Methods and compositions for increasing the activity of inhibitory RNA
US8268550B2 (en) * 2009-06-26 2012-09-18 Massachusetts Institute Of Technology Compositions and methods for identification of PARP function, inhibitors, and activators
KR101800878B1 (en) * 2010-03-31 2017-11-27 전남대학교 산학협력단 Methods for Analyzing a Genotoxicity Using Non-fluorescent Fluorescence Proteins
WO2011135040A1 (en) 2010-04-30 2011-11-03 F. Hoffmann-La Roche Ag Fluorescent antibody fusion protein, its production and use
US9134301B2 (en) * 2010-11-22 2015-09-15 Bio-Rad Laboratories, Inc. Sorting of adherent cells by selective transformation of labels
US9040248B2 (en) 2012-06-06 2015-05-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Kits for detecting and monitoring endocrine disrupting chemicals (EDCs)
US20140024066A1 (en) * 2012-06-20 2014-01-23 California Institute Of Technology Animal model having photo-activatable mitochondria
RU2602452C2 (en) * 2014-05-14 2016-11-20 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Method of substances and their compositions detection with anticancer activity
JPWO2022215532A1 (en) * 2021-04-07 2022-10-13

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5668750A (en) * 1995-07-28 1997-09-16 Nec Corporation Bipolar multiplier with wide input voltage range using multitail cell
US5874304A (en) * 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods
US6096865A (en) * 1996-05-06 2000-08-01 Amgen Inc. Mutants of the green fluorescent protein having improved fluorescent properties at 37°
WO2000034326A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
US6777079B2 (en) * 2000-12-01 2004-08-17 3M Innovative Properties Company Crosslinked pressure sensitive adhesive compositions, and adhesive articles based thereon, useful in high temperature applications
GB0109858D0 (en) * 2001-04-23 2001-06-13 Amersham Pharm Biotech Uk Ltd Fluorscent proteins
ES2340154T3 (en) 2002-01-22 2010-05-31 Evrogen Ip FLUORESCENT PROTEINS OF AEQUOREA COERULESCENS AND USE OF THE SAME.

Also Published As

Publication number Publication date
AU2003208520B2 (en) 2008-06-05
US7432053B2 (en) 2008-10-07
WO2003062270A2 (en) 2003-07-31
IL162568A0 (en) 2005-11-20
RU2004125585A (en) 2005-04-20
ES2340154T3 (en) 2010-05-31
RU2330886C2 (en) 2008-08-10
WO2003062270A8 (en) 2004-11-04
WO2003062270A3 (en) 2003-11-27
CA2474108A1 (en) 2003-07-31
DK1485481T3 (en) 2010-06-07
US20060167225A1 (en) 2006-07-27
EP1485481A2 (en) 2004-12-15
JP5465649B2 (en) 2014-04-09
US7879988B2 (en) 2011-02-01
US7897726B2 (en) 2011-03-01
CA2474108C (en) 2012-06-19
JP2005526495A (en) 2005-09-08
IL162568A (en) 2010-12-30
DE60331422D1 (en) 2010-04-08
JP2011087581A (en) 2011-05-06
JP2010046084A (en) 2010-03-04
US7667016B2 (en) 2010-02-23
EP1485481B1 (en) 2010-02-24
US20090148940A1 (en) 2009-06-11
JP4510464B2 (en) 2010-07-21
ATE458816T1 (en) 2010-03-15
US20090148939A1 (en) 2009-06-11
US20090137033A1 (en) 2009-05-28

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