WO2003062270A8 - Fluorescent protein from aequorea coerulscens and uses therof - Google Patents

Fluorescent protein from aequorea coerulscens and uses therof

Info

Publication number
WO2003062270A8
WO2003062270A8 PCT/IB2003/000907 IB0300907W WO03062270A8 WO 2003062270 A8 WO2003062270 A8 WO 2003062270A8 IB 0300907 W IB0300907 W IB 0300907W WO 03062270 A8 WO03062270 A8 WO 03062270A8
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescent
nucleic acid
fluorescent protein
acid compositions
coerulscens
Prior art date
Application number
PCT/IB2003/000907
Other languages
French (fr)
Other versions
WO2003062270A2 (en
WO2003062270B1 (en
WO2003062270A3 (en
Inventor
Nadejda Gurskaya
Arkadiy Fradlov
Sergey Lukyanov
Natalia Punkova
Original Assignee
Nadejda Gurskaya
Arkadiy Fradlov
Sergey Lukyanov
Natalia Punkova
Evrogen Jsc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AT03706812T priority Critical patent/ATE458816T1/en
Priority to JP2003562147A priority patent/JP4510464B2/en
Priority to IL16256803A priority patent/IL162568A0/en
Application filed by Nadejda Gurskaya, Arkadiy Fradlov, Sergey Lukyanov, Natalia Punkova, Evrogen Jsc filed Critical Nadejda Gurskaya
Priority to US10/501,629 priority patent/US7432053B2/en
Priority to DK03706812.9T priority patent/DK1485481T3/en
Priority to CA2474108A priority patent/CA2474108C/en
Priority to AU2003208520A priority patent/AU2003208520B2/en
Priority to EP03706812A priority patent/EP1485481B1/en
Priority to DE60331422T priority patent/DE60331422D1/en
Publication of WO2003062270A2 publication Critical patent/WO2003062270A2/en
Publication of WO2003062270A3 publication Critical patent/WO2003062270A3/en
Publication of WO2003062270B1 publication Critical patent/WO2003062270B1/en
Priority to IL162568A priority patent/IL162568A/en
Publication of WO2003062270A8 publication Critical patent/WO2003062270A8/en
Priority to US12/199,983 priority patent/US7667016B2/en
Priority to US12/200,064 priority patent/US7897726B2/en
Priority to US12/200,113 priority patent/US7879988B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

Abstract

The present invention provides nucleic acid compositions encoding a novel colorless GFP-like protein, acGFP, from Aequorea coerulscens and fluorescent and non-fluorescent mutants and derivatives thereof, as well as peptides and proteins encoded by these nucleic acid compositions. The subject protein and nucleic acid compositions of the present invention are colored and/or fluorescent and/or can be photoactivated, and can be used in a variety of different biological applications, particularly for labeling. Finally, kits for use in such biological applications are provided.
PCT/IB2003/000907 2002-01-22 2003-01-17 Fluorescent protein from aequorea coerulscens and uses therof WO2003062270A2 (en)

Priority Applications (13)

Application Number Priority Date Filing Date Title
DE60331422T DE60331422D1 (en) 2002-01-22 2003-01-17 FLUORESCENT PROTEIN FROM AEQUOREA COERULESCENS AND ITS USES
JP2003562147A JP4510464B2 (en) 2002-01-22 2003-01-17 Novel fluorescent protein derived from Aequorea coerulescens and method of use thereof
EP03706812A EP1485481B1 (en) 2002-01-22 2003-01-17 Fluorescent protein from aequorea coerulescens and uses thereof
US10/501,629 US7432053B2 (en) 2002-01-22 2003-01-17 Fluorescent protein from Aequorea coerulscens and methods for using the same
DK03706812.9T DK1485481T3 (en) 2002-01-22 2003-01-17 Fluorescent protein from Aequorea Coerulescens and uses thereof
CA2474108A CA2474108C (en) 2002-01-22 2003-01-17 Novel fluorescent proteins from aequorea coerulscens and methods for using same
AU2003208520A AU2003208520B2 (en) 2002-01-22 2003-01-17 Fluorescent protein from aequorea coerulscens and uses therof
AT03706812T ATE458816T1 (en) 2002-01-22 2003-01-17 FLUORESCENT PROTEIN FROM AEQUOREA COERULESCENS AND THEIR USES
IL16256803A IL162568A0 (en) 2002-01-22 2003-01-17 fluorescent protein from aequorea coerulscens and uses thereof
IL162568A IL162568A (en) 2002-01-22 2004-06-16 Isolated nucleic acid encoding a fluorescent mutant of an aequorea coerulescens protein
US12/199,983 US7667016B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same
US12/200,064 US7897726B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same
US12/200,113 US7879988B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35151802P 2002-01-22 2002-01-22
US60/351,518 2002-01-22

Related Child Applications (4)

Application Number Title Priority Date Filing Date
US10501629 A-371-Of-International 2003-01-17
US12/200,113 Division US7879988B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same
US12/200,064 Division US7897726B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same
US12/199,983 Division US7667016B2 (en) 2002-01-22 2008-08-28 Fluorescent proteins from Aequorea coerulscens and methods for using the same

Publications (4)

Publication Number Publication Date
WO2003062270A2 WO2003062270A2 (en) 2003-07-31
WO2003062270A3 WO2003062270A3 (en) 2003-11-27
WO2003062270B1 WO2003062270B1 (en) 2004-04-01
WO2003062270A8 true WO2003062270A8 (en) 2004-11-04

Family

ID=27613506

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2003/000907 WO2003062270A2 (en) 2002-01-22 2003-01-17 Fluorescent protein from aequorea coerulscens and uses therof

Country Status (12)

Country Link
US (4) US7432053B2 (en)
EP (1) EP1485481B1 (en)
JP (3) JP4510464B2 (en)
AT (1) ATE458816T1 (en)
AU (1) AU2003208520B2 (en)
CA (1) CA2474108C (en)
DE (1) DE60331422D1 (en)
DK (1) DK1485481T3 (en)
ES (1) ES2340154T3 (en)
IL (2) IL162568A0 (en)
RU (1) RU2330886C2 (en)
WO (1) WO2003062270A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2330886C2 (en) * 2002-01-22 2008-08-10 Закрытое Акционерное Общество "Евроген Айпи" NEW FLUORESCENT PROTEINS Aequorea coerulscens AND METHODS OF APPLICATION THEREOF
DE03781176T1 (en) 2002-12-26 2006-06-22 Zakrytoe Aktsionernoe Obschestvo "Evrogen" FLUORESCENCE PROTEINS FROM COPEPODA SPECIES AND METHOD FOR USE THEREOF
EP1820024B1 (en) 2004-12-01 2012-06-13 Proteologics, Ltd Ubiquitin ligase assays and related reagents
UA93922C2 (en) * 2009-03-20 2011-03-25 Михаил Викторович Разуменко Process for production of peptides, specifically recognizing the cells of particular type, meant for therapeutic goals
US8268550B2 (en) * 2009-06-26 2012-09-18 Massachusetts Institute Of Technology Compositions and methods for identification of PARP function, inhibitors, and activators
WO2010151664A2 (en) 2009-06-26 2010-12-29 Massachusetts Institute Of Technology Compositions and methods for treating cancer and modulating stress granule formation
US8435961B2 (en) 2009-06-26 2013-05-07 Massachusetts Institute Of Technology Methods and compositions for increasing the activity of inhibitory RNA
KR101800878B1 (en) * 2010-03-31 2017-11-27 전남대학교 산학협력단 Methods for Analyzing a Genotoxicity Using Non-fluorescent Fluorescence Proteins
WO2011135040A1 (en) 2010-04-30 2011-11-03 F. Hoffmann-La Roche Ag Fluorescent antibody fusion protein, its production and use
US9134301B2 (en) * 2010-11-22 2015-09-15 Bio-Rad Laboratories, Inc. Sorting of adherent cells by selective transformation of labels
US9040248B2 (en) 2012-06-06 2015-05-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Kits for detecting and monitoring endocrine disrupting chemicals (EDCs)
US20140024066A1 (en) * 2012-06-20 2014-01-23 California Institute Of Technology Animal model having photo-activatable mitochondria
RU2602452C2 (en) * 2014-05-14 2016-11-20 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Method of substances and their compositions detection with anticancer activity
EP4321539A1 (en) * 2021-04-07 2024-02-14 Riken Novel polypeptide exhibiting fluorescent properties and use for same

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5668750A (en) * 1995-07-28 1997-09-16 Nec Corporation Bipolar multiplier with wide input voltage range using multitail cell
US5874304A (en) * 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods
US6096865A (en) * 1996-05-06 2000-08-01 Amgen Inc. Mutants of the green fluorescent protein having improved fluorescent properties at 37°
WO2000034326A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
ATE317881T1 (en) * 2000-12-01 2006-03-15 3M Innovative Properties Co CROSS-LINKED ADHESIVE COMPOSITIONS AND ADHESIVE OBJECTS BASED THEREOF USABLE FOR HIGH TEMPERATURE APPLICATIONS
GB0109858D0 (en) * 2001-04-23 2001-06-13 Amersham Pharm Biotech Uk Ltd Fluorscent proteins
RU2330886C2 (en) 2002-01-22 2008-08-10 Закрытое Акционерное Общество "Евроген Айпи" NEW FLUORESCENT PROTEINS Aequorea coerulscens AND METHODS OF APPLICATION THEREOF

Also Published As

Publication number Publication date
EP1485481B1 (en) 2010-02-24
US20090148940A1 (en) 2009-06-11
JP2010046084A (en) 2010-03-04
ATE458816T1 (en) 2010-03-15
ES2340154T3 (en) 2010-05-31
JP4510464B2 (en) 2010-07-21
WO2003062270A2 (en) 2003-07-31
WO2003062270B1 (en) 2004-04-01
US7897726B2 (en) 2011-03-01
US7879988B2 (en) 2011-02-01
US20060167225A1 (en) 2006-07-27
CA2474108A1 (en) 2003-07-31
JP2011087581A (en) 2011-05-06
JP2005526495A (en) 2005-09-08
EP1485481A2 (en) 2004-12-15
DE60331422D1 (en) 2010-04-08
IL162568A0 (en) 2005-11-20
US20090137033A1 (en) 2009-05-28
US7432053B2 (en) 2008-10-07
IL162568A (en) 2010-12-30
DK1485481T3 (en) 2010-06-07
CA2474108C (en) 2012-06-19
US20090148939A1 (en) 2009-06-11
AU2003208520B2 (en) 2008-06-05
JP5465649B2 (en) 2014-04-09
RU2330886C2 (en) 2008-08-10
WO2003062270A3 (en) 2003-11-27
RU2004125585A (en) 2005-04-20
US7667016B2 (en) 2010-02-23

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