WO2003052134A3 - Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof - Google Patents
Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof Download PDFInfo
- Publication number
- WO2003052134A3 WO2003052134A3 PCT/DK2002/000877 DK0200877W WO03052134A3 WO 2003052134 A3 WO2003052134 A3 WO 2003052134A3 DK 0200877 W DK0200877 W DK 0200877W WO 03052134 A3 WO03052134 A3 WO 03052134A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- oligonucleotides
- target sequence
- methods
- nucleic acids
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/13—Decoys
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
- C12N2310/3181—Peptide nucleic acid, PNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/321—2'-O-R Modification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/323—Chemical structure of the sugar modified ring structure
- C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3511—Conjugate intercalating or cleaving agent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3517—Marker; Tag
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2002361946A AU2002361946A1 (en) | 2001-12-18 | 2002-12-18 | Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof |
Applications Claiming Priority (18)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200101900 | 2001-12-18 | ||
DKPA200101899 | 2001-12-18 | ||
DKPA200101900 | 2001-12-18 | ||
DKPA200101897 | 2001-12-18 | ||
DKPA200101897 | 2001-12-18 | ||
DKPA200101899 | 2001-12-18 | ||
DKPA200101898 | 2001-12-18 | ||
DKPA200101898 | 2001-12-18 | ||
US36554502P | 2002-03-20 | 2002-03-20 | |
US60/365,545 | 2002-03-20 | ||
DKPA200201575 | 2002-10-14 | ||
DKPA200201576 | 2002-10-14 | ||
DKPA200201577 | 2002-10-14 | ||
DKPA200201578 | 2002-10-14 | ||
DKPA200201577 | 2002-10-14 | ||
DKPA200201575 | 2002-10-14 | ||
DKPA200201578 | 2002-10-14 | ||
DKPA200201576 | 2002-10-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003052134A2 WO2003052134A2 (en) | 2003-06-26 |
WO2003052134A3 true WO2003052134A3 (en) | 2004-03-25 |
Family
ID=27576036
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK2002/000876 WO2003051901A2 (en) | 2001-12-18 | 2002-12-18 | Pseudonucleotide comprising an intercalator |
PCT/DK2002/000877 WO2003052134A2 (en) | 2001-12-18 | 2002-12-18 | Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof |
PCT/DK2002/000875 WO2003052133A2 (en) | 2001-12-18 | 2002-12-18 | Oligonucleotide analogues comprising intercalator pseudonucleotides |
PCT/DK2002/000874 WO2003052132A2 (en) | 2001-12-18 | 2002-12-18 | Linear and hairpin oligonucleotide analogues comprising intercalator pseudonucleotides |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK2002/000876 WO2003051901A2 (en) | 2001-12-18 | 2002-12-18 | Pseudonucleotide comprising an intercalator |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK2002/000875 WO2003052133A2 (en) | 2001-12-18 | 2002-12-18 | Oligonucleotide analogues comprising intercalator pseudonucleotides |
PCT/DK2002/000874 WO2003052132A2 (en) | 2001-12-18 | 2002-12-18 | Linear and hairpin oligonucleotide analogues comprising intercalator pseudonucleotides |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1468007A2 (en) |
CN (1) | CN1653079B (en) |
AU (4) | AU2002358464B2 (en) |
WO (4) | WO2003051901A2 (en) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
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AU2003900368A0 (en) * | 2003-01-24 | 2003-02-13 | Human Genetic Signatures Pty Ltd | Assay for nucleic acid molecules |
EP1594987B1 (en) | 2003-02-21 | 2009-05-27 | GeneForm Technologies Limited | Nucleic acid sequencing methods, kits and reagents |
WO2004101816A1 (en) * | 2003-05-15 | 2004-11-25 | Human Genetic Signatures Pty Ltd | Decoys for nucleic acid-binding proteins |
DE602004028478D1 (en) | 2003-06-17 | 2010-09-16 | Human Genetic Signatures Pty | PROCESS FOR GENOMA PLIFICATION |
EP1668148B1 (en) | 2003-09-04 | 2008-12-31 | Human Genetic Signatures PTY Ltd. | Nucleic acid detection assay |
JP4397659B2 (en) * | 2003-09-11 | 2010-01-13 | 独立行政法人科学技術振興機構 | DNA detection method using molecular beacon using switching between monomer emission and excimer emission of fluorescent molecules |
US8168777B2 (en) | 2004-04-29 | 2012-05-01 | Human Genetic Signatures Pty. Ltd. | Bisulphite reagent treatment of nucleic acid |
DK1794173T3 (en) | 2004-09-10 | 2010-10-25 | Human Genetic Signatures Pty | Amplification blocks containing intercalating nucleic acids (INA) containing intercalating pseudonucleotides (IPN) |
US7671184B2 (en) | 2004-09-24 | 2010-03-02 | Universitact Bern | Molecular beacons |
CN101111606B (en) | 2004-12-03 | 2012-05-16 | 人类遗传标记控股有限公司 | Methods for simplifying microbial nucleic acids by chemical modification of cytosines |
SI1888748T1 (en) * | 2005-05-25 | 2014-02-28 | Tina Holding Aps | Stable and selective formation of hoogsteen-type triplexes and duplexes using twisted intercalating nucleic acids (tina) and process for the preparation of tina |
CN101203618B (en) | 2005-05-26 | 2013-03-13 | 人类遗传标记控股有限公司 | Isothermal strand displacement amplification using primers containing a non-regular base |
US8343738B2 (en) | 2005-09-14 | 2013-01-01 | Human Genetic Signatures Pty. Ltd. | Assay for screening for potential cervical cancer |
CN101448957A (en) * | 2006-03-16 | 2009-06-03 | 潘塔贝斯公司 | Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids |
EP1845166A1 (en) * | 2006-04-13 | 2007-10-17 | BioSpring GmbH | Nucleic acid probes with covalently bound intercalating fluorescent dyes for hybridization with target nucleic acids |
US8299237B2 (en) | 2007-08-30 | 2012-10-30 | Hadasit Medical Research Services & Development Ltd. | Nucleic acid sequences comprising NF-κB binding site within O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter region and uses thereof for the treatment of cancer and immune-related disorders |
US8685675B2 (en) | 2007-11-27 | 2014-04-01 | Human Genetic Signatures Pty. Ltd. | Enzymes for amplification and copying bisulphite modified nucleic acids |
AU2008338530A1 (en) | 2007-12-14 | 2009-06-25 | Minitube Of America, Inc. | Gender-specific separation of sperm cells and embryos |
WO2011032034A2 (en) | 2009-09-10 | 2011-03-17 | University Of Idaho | Nucleobase-functionalized conformationally restricted nucleotides and oligonucleotides for targeting nucleic acids |
EP2566981A2 (en) | 2010-05-07 | 2013-03-13 | Quantibact A/s | Method for generating a double stranded nucleic acid with a single stranded overhang |
AR088140A1 (en) | 2011-07-19 | 2014-05-14 | Univ Idaho | PROBE AND METHOD FOR DIRECTING NUCLEIC ACIDS |
MY167564A (en) | 2011-09-07 | 2018-09-14 | Human Genetic Signatures Pty Ltd | Molecular detection assay |
WO2017045689A1 (en) | 2015-09-14 | 2017-03-23 | Pentabase Aps | Methods and materials for detection of mutations |
IL291323B2 (en) | 2016-07-15 | 2023-09-01 | Am Chemicals Llc | Non-nucleosidic solid supports and phosphoramidite building blocks for oligonucleotide synthesis |
CA3101215A1 (en) * | 2018-06-08 | 2019-12-12 | Carnegie Mellon University | Modified nucleobases with uniform h-bonding interactions, homo- and hetero-basepair bias, and mismatch discrimination |
WO2020010495A1 (en) * | 2018-07-09 | 2020-01-16 | 深圳华大智造极创科技有限公司 | Method for nucleic acid sequencing |
EP3969614A1 (en) | 2019-05-13 | 2022-03-23 | PentaBase ApS | Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids |
CN111073884A (en) * | 2020-02-14 | 2020-04-28 | 昆明理工大学 | Method for improving accuracy of SNP locus detection with functional effect in SNP with distance of non-coding region less than 50bp |
CA3183054A1 (en) | 2020-06-29 | 2022-01-06 | Pentabase Aps | Detection of methylation status |
Citations (6)
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US5414077A (en) * | 1990-02-20 | 1995-05-09 | Gilead Sciences | Non-nucleoside linkers for convenient attachment of labels to oligonucleotides using standard synthetic methods |
WO1997039008A1 (en) * | 1996-04-12 | 1997-10-23 | The Public Health Research Institute Of The City Of New York, Inc. | Detection probes, kits and assays |
WO1997043298A1 (en) * | 1996-05-15 | 1997-11-20 | Research Corporation Technologies, Inc. | Novel nucleoside analogs with polycyclic aromatic groups attached, methods of synthesis and uses therefor |
WO1998018965A1 (en) * | 1996-10-29 | 1998-05-07 | University Of Nebraska At Lincoln | Method for detecting point mutations in dna utilizing fluorescence energy transfer |
US6225052B1 (en) * | 1997-02-24 | 2001-05-01 | Roche Diagnostics Gmbh | Method for determining a nucleic acid |
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2002
- 2002-12-18 WO PCT/DK2002/000876 patent/WO2003051901A2/en not_active Application Discontinuation
- 2002-12-18 AU AU2002358464A patent/AU2002358464B2/en not_active Ceased
- 2002-12-18 EP EP02792708A patent/EP1468007A2/en not_active Withdrawn
- 2002-12-18 AU AU2002358462A patent/AU2002358462A1/en not_active Abandoned
- 2002-12-18 CN CN028282140A patent/CN1653079B/en not_active Expired - Fee Related
- 2002-12-18 AU AU2002361946A patent/AU2002361946A1/en not_active Abandoned
- 2002-12-18 WO PCT/DK2002/000877 patent/WO2003052134A2/en not_active Application Discontinuation
- 2002-12-18 WO PCT/DK2002/000875 patent/WO2003052133A2/en not_active Application Discontinuation
- 2002-12-18 WO PCT/DK2002/000874 patent/WO2003052132A2/en not_active Application Discontinuation
- 2002-12-18 AU AU2002358463A patent/AU2002358463A1/en not_active Abandoned
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US5332659A (en) * | 1992-04-09 | 1994-07-26 | The United States Of America As Represented By The Secretary Of The Navy | Light emission-or absorbance-based binding assays for polynucleic acids |
WO1997039008A1 (en) * | 1996-04-12 | 1997-10-23 | The Public Health Research Institute Of The City Of New York, Inc. | Detection probes, kits and assays |
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CHRISTENSEN U B ET AL: "Intercalating nucleic acids containing insertions of 1-O-(1-pyrenylmethyl)glycerol: stabilisation of dsDNA and discrimination of DNA over RNA", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 30, no. 22, 15 November 2002 (2002-11-15), pages 4918 - 4925, XP002246723, ISSN: 0305-1048 * |
DE MESMAEKER A ET AL: "Amide Backbone Modifications for Antisense Oligonucleotides Carrying Potential Intercalating Substituents: Influence on the Thermodynamic Stability of the Corresponding Duplexes with RNA- and DNA-Complements", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, OXFORD, GB, vol. 7, no. 14, 22 July 1997 (1997-07-22), pages 1869 - 1874, XP004136348, ISSN: 0960-894X * |
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Also Published As
Publication number | Publication date |
---|---|
WO2003052134A2 (en) | 2003-06-26 |
AU2002358463A1 (en) | 2003-06-30 |
AU2002358464B2 (en) | 2006-04-27 |
AU2002358462A1 (en) | 2003-06-30 |
AU2002358463A8 (en) | 2003-06-30 |
AU2002361946A1 (en) | 2003-06-30 |
AU2002358462A8 (en) | 2003-06-30 |
WO2003051901A3 (en) | 2003-11-27 |
WO2003052132A3 (en) | 2003-10-09 |
WO2003051901A2 (en) | 2003-06-26 |
CN1653079B (en) | 2010-06-16 |
CN1653079A (en) | 2005-08-10 |
WO2003052132A2 (en) | 2003-06-26 |
EP1468007A2 (en) | 2004-10-20 |
WO2003052133A3 (en) | 2003-10-02 |
WO2003052133A2 (en) | 2003-06-26 |
AU2002358464A1 (en) | 2003-06-30 |
AU2002361946A8 (en) | 2003-06-30 |
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