WO2003052108A3 - Improved methods and means for delivering inhibitory rna to plants and applications thereof - Google Patents

Improved methods and means for delivering inhibitory rna to plants and applications thereof Download PDF

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Publication number
WO2003052108A3
WO2003052108A3 PCT/EP2002/013964 EP0213964W WO03052108A3 WO 2003052108 A3 WO2003052108 A3 WO 2003052108A3 EP 0213964 W EP0213964 W EP 0213964W WO 03052108 A3 WO03052108 A3 WO 03052108A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
inhibitory rna
improved methods
applications
delivering inhibitory
Prior art date
Application number
PCT/EP2002/013964
Other languages
French (fr)
Other versions
WO2003052108A2 (en
Inventor
Michael H Metzlaff
Veronique M L Gossele
Frank Meulewaeter
Ina C A Fache
Original Assignee
Bayer Bioscience Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Bioscience Nv filed Critical Bayer Bioscience Nv
Priority to AU2002361997A priority Critical patent/AU2002361997B2/en
Priority to EP02796595A priority patent/EP1458876A2/en
Priority to CA002470426A priority patent/CA2470426A1/en
Publication of WO2003052108A2 publication Critical patent/WO2003052108A2/en
Publication of WO2003052108A3 publication Critical patent/WO2003052108A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation

Abstract

The invention provides improved methods and means for introducing inhibitory RNA into plant cells. The invention also provides kits comprising viral RNA vectors derived from satellite viruses and corresponding helper viruses for the introduction of inhibitory RNA into plant cells and plants. Further, the invention comprises methods for obtaining an enhanced or improved gene-silencing phenotype.
PCT/EP2002/013964 2001-12-18 2002-12-05 Improved methods and means for delivering inhibitory rna to plants and applications thereof WO2003052108A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2002361997A AU2002361997B2 (en) 2001-12-18 2002-12-05 Improved methods and means for delivering inhibitory RNA to plants and applications thereof
EP02796595A EP1458876A2 (en) 2001-12-18 2002-12-05 Improved methods and means for delivering inhibitory rna to plants and applications thereof
CA002470426A CA2470426A1 (en) 2001-12-18 2002-12-05 Improved methods and means for delivering inhibitory rna to plants and applications thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34048801P 2001-12-18 2001-12-18
US60/340,488 2001-12-18

Publications (2)

Publication Number Publication Date
WO2003052108A2 WO2003052108A2 (en) 2003-06-26
WO2003052108A3 true WO2003052108A3 (en) 2004-02-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/013964 WO2003052108A2 (en) 2001-12-18 2002-12-05 Improved methods and means for delivering inhibitory rna to plants and applications thereof

Country Status (5)

Country Link
US (1) US7485774B2 (en)
EP (1) EP1458876A2 (en)
AU (1) AU2002361997B2 (en)
CA (1) CA2470426A1 (en)
WO (1) WO2003052108A2 (en)

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US7566813B2 (en) 2002-03-21 2009-07-28 Monsanto Technology, L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
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EP1516051A4 (en) * 2002-06-21 2007-05-02 Monsanto Technology Llc Intron double stranded rna constructs and uses thereof
AU2003287622A1 (en) * 2002-11-06 2004-06-03 Fraunhofer Usa Expression of foreign sequences in plants using trans-activation system
US7683238B2 (en) * 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
US7692063B2 (en) * 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
EP2192172B1 (en) 2003-02-03 2014-12-03 iBio, Inc. System for expression of genes in plants
US7786349B2 (en) 2003-04-09 2010-08-31 Bayer Bioscience N.V. Methods and means for increasing the tolerance of plants to stress conditions
EP1769068B1 (en) * 2004-02-20 2014-12-31 iBio, Inc. Systems and methods for clonal expression in plants
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US8685679B2 (en) * 2004-11-04 2014-04-01 E I Du Pont De Nemours And Company Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
CN101421406B (en) 2006-02-13 2016-08-31 孟山都技术有限公司 For producing nucleic acid construct and the method that the seed oil of change forms
MX356687B (en) * 2008-09-29 2018-06-07 Monsanto Technology Llc Soybean transgenic event mon87705 and methods for detection thereof.
BR112012018443A8 (en) 2010-01-25 2019-01-29 Bayer Cropscience Nv methods for making plant cell walls comprising chitin
JP2013531502A (en) 2010-07-08 2013-08-08 バイエル・クロップサイエンス・エヌ・ヴェー Glucosinolate transport protein and use thereof
AU2011354240B2 (en) 2011-01-04 2016-12-08 Bayer Cropscience N.V. Fiber selective promoters
EP2668278A1 (en) 2011-01-24 2013-12-04 Bayer CropScience NV Use of the rd29 promoter or fragments thereof for stress-inducible expression of transgenes in cotton
AU2012238601B2 (en) 2011-04-07 2017-01-12 Bayer Cropscience Nv Seed - specific promoter in cotton
EP2742059A1 (en) 2011-08-12 2014-06-18 Bayer CropScience NV Guard cell-specific expression of transgenes in cotton
CN103981149A (en) 2011-08-22 2014-08-13 拜尔作物科学公司 Methods and means to modify a plant genome
BR112015017830A2 (en) 2013-01-29 2017-11-21 Bayer Cropscience Lp processes and means to increase stress tolerance and biomass in plants
EP3017049B1 (en) 2013-07-01 2018-08-22 Bayer CropScience NV Methods and means for modulating flowering time in monocot plants
PL3049517T3 (en) 2013-09-24 2018-10-31 Bayer Cropscience Nv Hetero-transglycosylase and uses thereof
WO2016050512A1 (en) 2014-10-03 2016-04-07 Bayer Cropscience Nv Methods and means for increasing stress tolerance and biomass in plants
WO2016113333A1 (en) 2015-01-16 2016-07-21 Bayer Cropscience Nv Leaf-preferential promoters and uses thereof
WO2016128519A1 (en) 2015-02-12 2016-08-18 Bayer Cropscience Nv Shoot apex-preferential promoters and uses thereof
WO2017060232A1 (en) 2015-10-08 2017-04-13 Bayer Cropscience Nv Seed-preferential promoters and uses thereof
CA3001932A1 (en) 2015-10-16 2017-04-20 Bayer Cropscience Nv Brassica plants with altered properties in seed production
AU2017249663B2 (en) 2016-04-11 2023-04-20 BASF Agricultural Solutions Seed US LLC Seed-specific and endosperm-preferential promoters and uses thereof
EP3443098A1 (en) 2016-04-11 2019-02-20 Bayer CropScience NV Seed-specific and endosperm-preferential promoters and uses thereof
BR112018071103A2 (en) 2016-04-13 2019-02-26 Bayer Cropscience Nv embryo-specific and preferred seed promoters and uses thereof
WO2017178367A1 (en) 2016-04-13 2017-10-19 Bayer Cropscience Nv Seed- and funiculus-preferential promoters and uses thereof
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Also Published As

Publication number Publication date
WO2003052108A2 (en) 2003-06-26
CA2470426A1 (en) 2003-06-26
AU2002361997B8 (en) 2003-06-30
EP1458876A2 (en) 2004-09-22
AU2002361997B2 (en) 2007-10-04
US20030135882A1 (en) 2003-07-17
US7485774B2 (en) 2009-02-03
AU2002361997A1 (en) 2003-06-30

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