WO2003046213A3 - Method for concentrating and detecting hiv - Google Patents

Method for concentrating and detecting hiv Download PDF

Info

Publication number
WO2003046213A3
WO2003046213A3 PCT/EP2002/011931 EP0211931W WO03046213A3 WO 2003046213 A3 WO2003046213 A3 WO 2003046213A3 EP 0211931 W EP0211931 W EP 0211931W WO 03046213 A3 WO03046213 A3 WO 03046213A3
Authority
WO
WIPO (PCT)
Prior art keywords
concentrating
detecting hiv
viruses
detecting
lentiviruses
Prior art date
Application number
PCT/EP2002/011931
Other languages
German (de)
French (fr)
Other versions
WO2003046213A2 (en
Inventor
Uwe Michelsen
Achim Schwaemmle
Willi Roth
Original Assignee
Merck Patent Gmbh
Uwe Michelsen
Achim Schwaemmle
Willi Roth
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh, Uwe Michelsen, Achim Schwaemmle, Willi Roth filed Critical Merck Patent Gmbh
Priority to AU2002351787A priority Critical patent/AU2002351787A1/en
Publication of WO2003046213A2 publication Critical patent/WO2003046213A2/en
Publication of WO2003046213A3 publication Critical patent/WO2003046213A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The invention relates to a method and a test kit for enriching and detecting lentiviruses, especially HIV (Human Immunodeficiency Viruses) in complex biological samples e.g. body fluids. The viruses are bonded in suitable conditions to a solid phase, isolated, lysed and subsequently the viral nucleic acids are separated and optionally detected.
PCT/EP2002/011931 2001-11-26 2002-10-25 Method for concentrating and detecting hiv WO2003046213A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002351787A AU2002351787A1 (en) 2001-11-26 2002-10-25 Method for concentrating and detecting hiv

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10157624A DE10157624A1 (en) 2001-11-26 2001-11-26 Methods for HIV enrichment and detection
DE10157624.2 2001-11-26

Publications (2)

Publication Number Publication Date
WO2003046213A2 WO2003046213A2 (en) 2003-06-05
WO2003046213A3 true WO2003046213A3 (en) 2003-10-02

Family

ID=7706795

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/011931 WO2003046213A2 (en) 2001-11-26 2002-10-25 Method for concentrating and detecting hiv

Country Status (3)

Country Link
AU (1) AU2002351787A1 (en)
DE (1) DE10157624A1 (en)
WO (1) WO2003046213A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10258258A1 (en) * 2002-12-13 2004-06-24 Merck Patent Gmbh Isolating nucleic acids from liquid samples, for isolating viral nucleic acid from donated blood, by retention on an inorganic oxide carrier, comprises treatment with protease to improve sensitivity and efficiency
WO2005093065A1 (en) * 2004-03-16 2005-10-06 Roche Diagnostics Gmbh Improved method of isolating nucleic acids
JPWO2006123781A1 (en) * 2005-05-20 2008-12-25 アークレイ株式会社 Microbial and nucleic acid recovery method using fine particles and kit used therefor
DE102006032610C5 (en) * 2006-07-11 2016-09-15 Aj Innuscreen Gmbh Method for the parallel isolation of viral nucleic acids
US10131935B2 (en) 2006-07-11 2018-11-20 Aj Innuscreen Gmbh Method for parallel isolation of viral nucleic acids

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5681946A (en) * 1990-02-13 1997-10-28 Amersham International Plc Precipitating polymers
EP0897978A2 (en) * 1997-08-22 1999-02-24 Becton, Dickinson and Company Zirconium oxide and related compounds for purification of nucleic acids
WO1999040098A1 (en) * 1998-02-04 1999-08-12 Merck Patent Gmbh Method for isolating and purifying nucleic acids
US5990302A (en) * 1996-07-12 1999-11-23 Toyo Boseki Kabushiki Kaisha Method for isolating ribonucleic acid

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5681946A (en) * 1990-02-13 1997-10-28 Amersham International Plc Precipitating polymers
US5990302A (en) * 1996-07-12 1999-11-23 Toyo Boseki Kabushiki Kaisha Method for isolating ribonucleic acid
EP0897978A2 (en) * 1997-08-22 1999-02-24 Becton, Dickinson and Company Zirconium oxide and related compounds for purification of nucleic acids
WO1999040098A1 (en) * 1998-02-04 1999-08-12 Merck Patent Gmbh Method for isolating and purifying nucleic acids

Also Published As

Publication number Publication date
AU2002351787A8 (en) 2003-06-10
WO2003046213A2 (en) 2003-06-05
DE10157624A1 (en) 2003-06-05
AU2002351787A1 (en) 2003-06-10

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