WO2003037914A3 - High throughput isolation of proteins by charge induction chromatography - Google Patents

High throughput isolation of proteins by charge induction chromatography Download PDF

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Publication number
WO2003037914A3
WO2003037914A3 PCT/DK2002/000717 DK0200717W WO03037914A3 WO 2003037914 A3 WO2003037914 A3 WO 2003037914A3 DK 0200717 W DK0200717 W DK 0200717W WO 03037914 A3 WO03037914 A3 WO 03037914A3
Authority
WO
WIPO (PCT)
Prior art keywords
library
biological compounds
materials
high throughput
tag
Prior art date
Application number
PCT/DK2002/000717
Other languages
French (fr)
Other versions
WO2003037914A2 (en
Inventor
Henrik Frisner
Lars Lehmann Hylli Christensen
Original Assignee
Novozymes As
Henrik Frisner
Lars Lehmann Hylli Christensen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As, Henrik Frisner, Lars Lehmann Hylli Christensen filed Critical Novozymes As
Priority to EP02774472A priority Critical patent/EP1442048A2/en
Priority to AU2002340776A priority patent/AU2002340776A1/en
Priority to US10/493,842 priority patent/US20050086822A1/en
Priority to CA002465543A priority patent/CA2465543A1/en
Publication of WO2003037914A2 publication Critical patent/WO2003037914A2/en
Publication of WO2003037914A3 publication Critical patent/WO2003037914A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Abstract

A method for high throughput micro-purification of a library of tag-free biological compounds is claimed wherein a population of discrete liquid samples comprising the library of tag-free biological compounds is contacted with a solid chromatographic material comprising a functional group selected from ion exchange materials, hydrophobic materials, affinity materials and hydrophobic charge induction materials so as to retain the tag-free biological compounds of the library, isolating the chromatographic material retaining the biological compounds, releasing the biological compounds of the library from the solid chromatographic material and collecting the released biological compounds of the library to produce a population of samples comprising the isolated biological compound.
PCT/DK2002/000717 2001-10-30 2002-10-29 High throughput isolation of proteins by charge induction chromatography WO2003037914A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP02774472A EP1442048A2 (en) 2001-10-30 2002-10-29 High throughput isolation of proteins by charge induction chromatography
AU2002340776A AU2002340776A1 (en) 2001-10-30 2002-10-29 High throughput isolation of proteins by charge induction chromatography
US10/493,842 US20050086822A1 (en) 2001-10-30 2002-10-29 High throughput isolation of biological compounds
CA002465543A CA2465543A1 (en) 2001-10-30 2002-10-29 High throughput isolation of biological compounds

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200101598 2001-10-30
DKPA200101598 2001-10-30

Publications (2)

Publication Number Publication Date
WO2003037914A2 WO2003037914A2 (en) 2003-05-08
WO2003037914A3 true WO2003037914A3 (en) 2004-03-25

Family

ID=8160798

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2002/000717 WO2003037914A2 (en) 2001-10-30 2002-10-29 High throughput isolation of proteins by charge induction chromatography

Country Status (5)

Country Link
US (1) US20050086822A1 (en)
EP (1) EP1442048A2 (en)
AU (1) AU2002340776A1 (en)
CA (1) CA2465543A1 (en)
WO (1) WO2003037914A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
KR101228356B1 (en) * 2004-04-08 2013-02-05 바이오매트리카 인코포레이티드 Integration of sample storage and sample management for life science
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
US7877895B2 (en) * 2006-06-26 2011-02-01 Tokyo Electron Limited Substrate processing apparatus
EP2598661B1 (en) 2010-07-26 2017-09-27 Biomatrica, INC. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
EP2598660B1 (en) 2010-07-26 2017-03-15 Biomatrica, INC. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US20140154233A1 (en) 2012-12-05 2014-06-05 Csl Limited Method of purifying therapeutic proteins
US10188965B2 (en) 2012-12-05 2019-01-29 Csl Behring Gmbh Hydrophobic charge induction chromatographic depletion of a protein from a solution
EP2934572A4 (en) 2012-12-20 2016-11-23 Biomatrica Inc Formulations and methods for stabilizing pcr reagents
EP3942931A1 (en) 2014-06-10 2022-01-26 Biomatrica, INC. Stabilization of thrombocytes at ambient temperatures
EP3387411B1 (en) 2015-12-08 2023-05-10 Biomatrica, INC. Reduction of erythrocyte sedimentation rate
CN107999027A (en) * 2017-12-06 2018-05-08 苏州博进生物技术有限公司 A kind of high mechanical properties affinity chromatography medium

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2003018596A2 (en) * 2001-08-27 2003-03-06 Tanox, Inc. Method for removing endotoxins from protein solutions

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003018596A2 (en) * 2001-08-27 2003-03-06 Tanox, Inc. Method for removing endotoxins from protein solutions

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BIOSEPARATION (2000), 9(4), 211-221, 2000 *
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; BOSCHETTI, EGISTO ET AL: "Purification of antibodies by HCIC and impact of ligand structure", XP002238463, retrieved from STN Database accession no. 137:199823 CA *
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; GUERRIER, L. ET AL: "New method for the selective capture of antibodies under physiological conditions", XP002207091, retrieved from STN Database accession no. 134:365403 CA *
G T WEATHERLY ET AL.: "Initial purification of recombinant botulinum neurotoxin fragments for pharmaceutical production using hydrophobic charge induction chromatography", JOURNAL OF CHROMATOGRAPHY A, vol. 952, no. 1-2, April 2002 (2002-04-01), SCIENCE NL, pages 99 - 110, XP004346350 *
INTERNATIONAL JOURNAL OF BIO-CHROMATOGRAPHY (2001), 6(4), 269-283, 2001 *
L GUERRIER ET AL.: "A dual-mode approach to the selective separation of antibodies and their fragments", JOURNAL OF CHROMATOGRAPHY B : BIOMEDICAL APPLICATIONS., vol. 755, 2001, ELSEVIER SCIENCE PUBLISHERS, NL, pages 37 - 46, XP004234475, ISSN: 0378-4347 *
S C BURTON & D R K HARDING: "Hydrophobic charge induction chromatography; salt independent protein adsorption and facile elution from aqueous buffers", JOURNAL OF CHROMATOGRAPHY A., vol. 814, no. 1-2, 1998, ELSEVIER SCIENCE., NL, pages 71 - 81, XP004145777, ISSN: 0021-9673 *
W SCHWARTZ ET AL.: "Comparison of hydrophobic charge induction chromatography with affinity chromatography on protein A for harvest and purification of antibodies", JOURNAL OF CHROMATOGRAPHY A., vol. 908, no. 1-2, 26 January 2001 (2001-01-26), ELSEVIER SCIENCE., NL, pages 251 - 263, XP004134138, ISSN: 0021-9673 *

Also Published As

Publication number Publication date
US20050086822A1 (en) 2005-04-28
AU2002340776A1 (en) 2003-05-12
WO2003037914A2 (en) 2003-05-08
CA2465543A1 (en) 2003-05-08
EP1442048A2 (en) 2004-08-04

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