WO2003031976A3 - Calibrating microarrays - Google Patents

Calibrating microarrays Download PDF

Info

Publication number
WO2003031976A3
WO2003031976A3 PCT/GB2002/004593 GB0204593W WO03031976A3 WO 2003031976 A3 WO2003031976 A3 WO 2003031976A3 GB 0204593 W GB0204593 W GB 0204593W WO 03031976 A3 WO03031976 A3 WO 03031976A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
ligand
affinity
sample
amount
Prior art date
Application number
PCT/GB2002/004593
Other languages
French (fr)
Other versions
WO2003031976A2 (en
Inventor
John Victor Lamont
Robert Ivan Mcconnell
Stephen Peter Fitzgerald
Maria Luz Rodriguez
Original Assignee
Randox Lab Ltd
John Victor Lamont
Robert Ivan Mcconnell
Stephen Peter Fitzgerald
Maria Luz Rodriguez
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Randox Lab Ltd, John Victor Lamont, Robert Ivan Mcconnell, Stephen Peter Fitzgerald, Maria Luz Rodriguez filed Critical Randox Lab Ltd
Priority to JP2003534908A priority Critical patent/JP4920173B2/en
Priority to CN02822956.8A priority patent/CN1589404B/en
Priority to US10/491,811 priority patent/US7846713B2/en
Priority to EP02767701A priority patent/EP1434995A2/en
Publication of WO2003031976A2 publication Critical patent/WO2003031976A2/en
Publication of WO2003031976A3 publication Critical patent/WO2003031976A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/809—Multifield plates or multicontainer arrays

Abstract

An assay for measuring the amount of a first analyte in a sample, comprises the steps of: (i) contacting the sample with a device that comprises one or more first reaction sites which comprise a first ligand having affinity for the first analyte, and a series of second reaction sites each comprising different known concentrations of an immobilised second analyte; (ii) removing any unbound first analyte; (iii) contacting the device with a second ligand that is detectably labelled and which has affinity for the first analyte, and a third ligand that is detectably labelled and which has affinity for the second analyte; (iv) removing any unbound second and third ligands; and (v) measuring the amount of second and third ligands, wherein measurement of the third ligand is used to establish a calibration curve, used to determine the amount of first analyte present in sample.
PCT/GB2002/004593 2001-10-10 2002-10-10 Calibrating microarrays WO2003031976A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2003534908A JP4920173B2 (en) 2001-10-10 2002-10-10 Calibration microarray
CN02822956.8A CN1589404B (en) 2001-10-10 2002-10-10 Analytical method for measuring content of the first analyte of smaple
US10/491,811 US7846713B2 (en) 2001-10-10 2002-10-10 Calibrating microarrays
EP02767701A EP1434995A2 (en) 2001-10-10 2002-10-10 Calibrating microarrays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0124338.5 2001-10-10
GBGB0124338.5A GB0124338D0 (en) 2001-10-10 2001-10-10 Biochips

Publications (2)

Publication Number Publication Date
WO2003031976A2 WO2003031976A2 (en) 2003-04-17
WO2003031976A3 true WO2003031976A3 (en) 2003-11-27

Family

ID=9923577

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/004593 WO2003031976A2 (en) 2001-10-10 2002-10-10 Calibrating microarrays

Country Status (6)

Country Link
US (1) US7846713B2 (en)
EP (1) EP1434995A2 (en)
JP (1) JP4920173B2 (en)
CN (1) CN1589404B (en)
GB (1) GB0124338D0 (en)
WO (1) WO2003031976A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1289904C (en) 2003-08-01 2006-12-13 博奥生物有限公司 Micro array reaction unit and its use
CA2475240A1 (en) * 2004-07-20 2006-01-20 Biophys, Inc. Method and device to measure dynamic internal calibration true dose response curves
US20140031249A1 (en) * 2004-07-20 2014-01-30 Sqi Diagnostics Systems Inc. Multiplex measure of isotype antigen response
CA2475456A1 (en) 2004-07-20 2006-01-20 Biophys, Inc. Method and device to optimize analyte and antibody substrate binding by least energy adsorption
US7939342B2 (en) 2005-03-30 2011-05-10 Kimberly-Clark Worldwide, Inc. Diagnostic test kits employing an internal calibration system
US7829347B2 (en) 2005-08-31 2010-11-09 Kimberly-Clark Worldwide, Inc. Diagnostic test kits with improved detection accuracy
EP2047269B1 (en) * 2006-07-14 2014-04-30 Eötvös Lorand University Measurement of complement activation products on antigen microarrays
CN102460170B (en) * 2009-04-20 2014-11-05 环球生物研究株式会社 Tube for measuring bio-related substance and quantifying system
GB201211634D0 (en) 2012-06-28 2012-08-15 Randox Lab Assay for benzylpiperazine and metabolites
EP3023792A1 (en) * 2014-11-18 2016-05-25 Biosims Technologies Spot control

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2324866A (en) * 1997-04-21 1998-11-04 Randox Lab Ltd Device for multianalyte assays.
WO2001009607A1 (en) * 1999-07-30 2001-02-08 Large Scale Proteomics, Corp. Microarrays and their manufacture
WO2001020330A1 (en) * 1999-09-17 2001-03-22 The Texas A & M University System Spatially addressed lipid bilayer arrays and lipid bilayers with addressable confined aqueous compartments

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US4459360A (en) 1981-10-05 1984-07-10 Mast Medical Industries, Ltd. Multiple-component binding assay system and method of making and using it
US5200051A (en) * 1988-11-14 1993-04-06 I-Stat Corporation Wholly microfabricated biosensors and process for the manufacture and use thereof
US5726064A (en) * 1990-11-22 1998-03-10 Applied Research Systems Ars Holding Nv Method of assay having calibration within the assay
US5677196A (en) * 1993-05-18 1997-10-14 University Of Utah Research Foundation Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
US7323298B1 (en) * 1994-06-17 2008-01-29 The Board Of Trustees Of The Leland Stanford Junior University Microarray for determining the relative abundances of polynuceotide sequences
US5599668A (en) * 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
WO1996028538A1 (en) * 1995-03-10 1996-09-19 Meso Scale Technologies, Llc Multi-array, multi-specific electrochemiluminescence testing
EP0904542B1 (en) * 1996-03-01 2005-06-29 Beckman Coulter, Inc. System for simultaneously conducting multiple ligand binding assays
AU3568897A (en) 1996-06-07 1998-01-05 Eos Biotechnology, Inc. Immobilised linear oligonucleotide arrays
US5780243A (en) * 1996-06-28 1998-07-14 Thaco Research, Ltd. Methods for the quantitative analysis of organic compounds
DE19731465A1 (en) * 1997-07-22 1999-01-28 Boehringer Mannheim Gmbh Use of control areas for the detection of interference samples in a detection procedure
CA2301539C (en) 1997-09-11 2003-06-17 Genovations, Inc. Method of making high density arrays
DE69731629T2 (en) * 1997-09-11 2005-11-03 Randox Laboratories Ltd., Crumlin Method and device for image analysis
US6232066B1 (en) * 1997-12-19 2001-05-15 Neogen, Inc. High throughput assay system
US6232608B1 (en) * 1998-08-18 2001-05-15 Molecular Devices Corporation Optimization systems in a scanning fluorometer
GB9923146D0 (en) * 1999-09-30 1999-12-01 Imperial College Detector array
ATE418733T1 (en) * 2000-05-04 2009-01-15 Blood Res Center COLLOID COMPOSITIONS FOR SOLID PHASE BIOMOLECULAR ANALYTICAL SYSTEMS
EP1287360A2 (en) 2000-06-02 2003-03-05 Zeptosens AG Kit and method for determining a plurality of analytes
CA2314398A1 (en) * 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US6916621B2 (en) * 2002-03-27 2005-07-12 Spectral Genomics, Inc. Methods for array-based comparitive binding assays
US20030153013A1 (en) * 2002-11-07 2003-08-14 Ruo-Pan Huang Antibody-based protein array system

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2324866A (en) * 1997-04-21 1998-11-04 Randox Lab Ltd Device for multianalyte assays.
WO2001009607A1 (en) * 1999-07-30 2001-02-08 Large Scale Proteomics, Corp. Microarrays and their manufacture
WO2001020330A1 (en) * 1999-09-17 2001-03-22 The Texas A & M University System Spatially addressed lipid bilayer arrays and lipid bilayers with addressable confined aqueous compartments

Also Published As

Publication number Publication date
JP4920173B2 (en) 2012-04-18
EP1434995A2 (en) 2004-07-07
US20040241700A1 (en) 2004-12-02
CN1589404B (en) 2014-08-20
WO2003031976A2 (en) 2003-04-17
CN1589404A (en) 2005-03-02
GB0124338D0 (en) 2001-11-28
US7846713B2 (en) 2010-12-07
JP2005504988A (en) 2005-02-17

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