WO2003027258A3 - RECOMBINANT DNA PROCESSES USING A dNTP MIXTURE CONTAINING MODIFIED NUCLEOTIDES - Google Patents

RECOMBINANT DNA PROCESSES USING A dNTP MIXTURE CONTAINING MODIFIED NUCLEOTIDES Download PDF

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Publication number
WO2003027258A3
WO2003027258A3 PCT/US2002/030943 US0230943W WO03027258A3 WO 2003027258 A3 WO2003027258 A3 WO 2003027258A3 US 0230943 W US0230943 W US 0230943W WO 03027258 A3 WO03027258 A3 WO 03027258A3
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WO
WIPO (PCT)
Prior art keywords
modified nucleotides
recombinant dna
mixture containing
containing modified
processes
Prior art date
Application number
PCT/US2002/030943
Other languages
French (fr)
Other versions
WO2003027258A2 (en
Inventor
Brian Ward
Leanne M Snyder
Chuan Li
Keming Song
Kristen E Opper
Stephanie Uder
Ron Hernan
Original Assignee
Sigma Aldrich Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26670194&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2003027258(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Sigma Aldrich Co filed Critical Sigma Aldrich Co
Priority to EP02768916A priority Critical patent/EP1446475A4/en
Priority to AU2002331920A priority patent/AU2002331920A1/en
Publication of WO2003027258A2 publication Critical patent/WO2003027258A2/en
Publication of WO2003027258A3 publication Critical patent/WO2003027258A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Abstract

Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.
PCT/US2002/030943 2001-09-28 2002-09-27 RECOMBINANT DNA PROCESSES USING A dNTP MIXTURE CONTAINING MODIFIED NUCLEOTIDES WO2003027258A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02768916A EP1446475A4 (en) 2001-09-28 2002-09-27 RECOMBINANT DNA PROCESSES USING A dNTP MIXTURE CONTAINING MODIFIED NUCLEOTIDES
AU2002331920A AU2002331920A1 (en) 2001-09-28 2002-09-27 Recombinant dna processes using a dntp mixture containing modified nucleotides

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32561201P 2001-09-28 2001-09-28
US60/325,612 2001-09-28
US10/002,292 2001-11-15
US10/002,292 US6902914B2 (en) 2001-09-28 2001-11-15 Recombinant DNA processes using a dNTP mixture containing modified nucleotides

Publications (2)

Publication Number Publication Date
WO2003027258A2 WO2003027258A2 (en) 2003-04-03
WO2003027258A3 true WO2003027258A3 (en) 2003-11-13

Family

ID=26670194

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/030943 WO2003027258A2 (en) 2001-09-28 2002-09-27 RECOMBINANT DNA PROCESSES USING A dNTP MIXTURE CONTAINING MODIFIED NUCLEOTIDES

Country Status (4)

Country Link
US (3) US6902914B2 (en)
EP (1) EP1446475A4 (en)
AU (1) AU2002331920A1 (en)
WO (1) WO2003027258A2 (en)

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US8691300B2 (en) 2009-08-28 2014-04-08 Mary Kay Inc. Skin care formulations

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CA3123249A1 (en) 2006-01-19 2007-07-26 Mary Kay, Inc. Skin care compositions comprising kakadu plum extract and acai berry extract
ES2700606T3 (en) 2006-04-28 2019-02-18 Igor Kutyavin Use of modified deoxynucleoside triphosphates in their bases to improve the detection of nucleic acids
EP2147010A1 (en) 2007-04-18 2010-01-27 Roche Diagnostics GmbH Nucleotide with an alpha-phosphate mimetic
RU2010142289A (en) * 2008-03-17 2012-04-27 Экспрессив Рисерч Б.В. (Nl) DETECTION OF EXPRESSION-related GENES
KR101417989B1 (en) * 2013-02-08 2014-07-09 주식회사 엔지노믹스 Method for regulating length of overhang of double stranded DNA
CN105358709B (en) * 2013-03-15 2018-12-07 雅培分子公司 System and method for detecting genome copy numbers variation
DK3102722T3 (en) 2014-02-04 2020-11-16 Jumpcode Genomics Inc THROUGH FRACTIONING
US20180362965A1 (en) * 2017-02-22 2018-12-20 Ingenza Ltd. Method for assembly of polynucleic acid sequences using phosphorothioate bonds within linker oligos
US11142788B2 (en) 2017-06-13 2021-10-12 Genetics Research, Llc Isolation of target nucleic acids
US10527608B2 (en) 2017-06-13 2020-01-07 Genetics Research, Llc Methods for rare event detection
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
US10947599B2 (en) 2017-06-13 2021-03-16 Genetics Research, Llc Tumor mutation burden
WO2019014359A2 (en) * 2017-07-12 2019-01-17 The Scripps Research Institute Polymerase chain transcription (pct): exponential synthesis of rna and modified rna
WO2022035950A2 (en) * 2020-08-12 2022-02-17 Jumpcode Genomics, Inc. Methods for targeted depletion of nucleic acids

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US5659027A (en) * 1989-12-20 1997-08-19 University Of North Carolina Boronated compounds
US5683869A (en) * 1993-09-03 1997-11-04 Duke University Method of nucleic acid sequencing
US5859231A (en) * 1993-09-03 1999-01-12 Duke University Synthesis of oligonucleotides with boranophosphonate linkages
US6495318B2 (en) * 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
US6087103A (en) * 1998-03-04 2000-07-11 Lifespan Biosciences, Inc. Tagged ligand arrays for identifying target-ligand interactions
US6087112A (en) * 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691300B2 (en) 2009-08-28 2014-04-08 Mary Kay Inc. Skin care formulations
US8895082B2 (en) 2009-08-28 2014-11-25 Mary Kay Inc. Skin care formulations

Also Published As

Publication number Publication date
US20050208564A1 (en) 2005-09-22
AU2002331920A1 (en) 2003-04-07
US7504238B2 (en) 2009-03-17
EP1446475A2 (en) 2004-08-18
WO2003027258A2 (en) 2003-04-03
US6902914B2 (en) 2005-06-07
US20030099956A1 (en) 2003-05-29
EP1446475A4 (en) 2007-11-07
US20060003353A1 (en) 2006-01-05

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