WO2003025202A3 - Engineered templates and their use in single primer amplification - Google Patents

Engineered templates and their use in single primer amplification Download PDF

Info

Publication number
WO2003025202A3
WO2003025202A3 PCT/US2002/029889 US0229889W WO03025202A3 WO 2003025202 A3 WO2003025202 A3 WO 2003025202A3 US 0229889 W US0229889 W US 0229889W WO 03025202 A3 WO03025202 A3 WO 03025202A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide
template
primer
anneals
predetermined sequence
Prior art date
Application number
PCT/US2002/029889
Other languages
French (fr)
Other versions
WO2003025202A2 (en
Inventor
Katherine S Bowdish
Shana Frederickson
Toshiaki Maruyama
Ying-Chi Lin
Mark Renshaw
Original Assignee
Alexion Pharma Inc
Katherine S Bowdish
Shana Frederickson
Toshiaki Maruyama
Ying-Chi Lin
Mark Renshaw
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharma Inc, Katherine S Bowdish, Shana Frederickson, Toshiaki Maruyama, Ying-Chi Lin, Mark Renshaw filed Critical Alexion Pharma Inc
Priority to CA2460639A priority Critical patent/CA2460639C/en
Priority to EP02775901A priority patent/EP1436404B1/en
Priority to DK02775901.8T priority patent/DK1436404T3/en
Priority to AU2002341752A priority patent/AU2002341752B2/en
Priority to JP2003529974A priority patent/JP4439262B2/en
Priority to DE60234369T priority patent/DE60234369D1/en
Priority to AT02775901T priority patent/ATE448320T1/en
Publication of WO2003025202A2 publication Critical patent/WO2003025202A2/en
Publication of WO2003025202A3 publication Critical patent/WO2003025202A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Abstract

Methods of amplifying nucleic acid have now been discovered which include the steps of: a) annealing a primer to a template nucleic acid sequence, the primer having a first portion which anneals to the template and a second portion of predetermined sequence; b) synthesizing a polynucleotide that anneals to and is complementary to the portion of the template between the location at which the first portion of the primer anneals to the template and the end of the template, the polynucleotide having a first end and a second end, wherein the first end incorporates the primer; c) separating the polynucleotide synthesized in step (b ) from the template; d) annealing a nested oligonucleotide to the second end of the polynucleotide synthesized in step (b), the nested oligonucleotide having a first portion that anneals to the second end of the polynucleotide and a second portion having the same predetermined sequence as the second portion of the primer; e) extending the polynucleotide synthesized in step (b) to provide a terminal portion thereof that is complementary to the predetermined sequence; and f) amplifying the extended polynucleotide using a single primer having the predetermined sequence.
PCT/US2002/029889 2001-09-19 2002-09-19 Engineered templates and their use in single primer amplification WO2003025202A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA2460639A CA2460639C (en) 2001-09-19 2002-09-19 Engineered templates and their use in single primer amplification
EP02775901A EP1436404B1 (en) 2001-09-19 2002-09-19 Engineered templates and their use in single primer amplification
DK02775901.8T DK1436404T3 (en) 2001-09-19 2002-09-19 Manipulated templates and their use in single-primer amplification
AU2002341752A AU2002341752B2 (en) 2001-09-19 2002-09-19 Engineered templates and their use in single primer amplification
JP2003529974A JP4439262B2 (en) 2001-09-19 2002-09-19 Engineered templates and their use in single primer amplification
DE60234369T DE60234369D1 (en) 2001-09-19 2002-09-19 MANIPULATED MATRICES AND THEIR USE IN SINGLE PRIMER AMPLIFICATION
AT02775901T ATE448320T1 (en) 2001-09-19 2002-09-19 MANIPULATED MATRICES AND THEIR USE IN SINGLE-PRIMER AMPLIFICATION

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32345501P 2001-09-19 2001-09-19
US60/323,455 2001-09-19

Publications (2)

Publication Number Publication Date
WO2003025202A2 WO2003025202A2 (en) 2003-03-27
WO2003025202A3 true WO2003025202A3 (en) 2004-02-12

Family

ID=23259271

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/029889 WO2003025202A2 (en) 2001-09-19 2002-09-19 Engineered templates and their use in single primer amplification

Country Status (12)

Country Link
US (1) US7306906B2 (en)
EP (1) EP1436404B1 (en)
JP (2) JP4439262B2 (en)
AT (1) ATE448320T1 (en)
AU (1) AU2002341752B2 (en)
CA (1) CA2460639C (en)
CY (1) CY1109828T1 (en)
DE (1) DE60234369D1 (en)
DK (1) DK1436404T3 (en)
ES (1) ES2336433T3 (en)
PT (1) PT1436404E (en)
WO (1) WO2003025202A2 (en)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7408041B2 (en) 2000-12-08 2008-08-05 Alexion Pharmaceuticals, Inc. Polypeptides and antibodies derived from chronic lymphocytic leukemia cells and uses thereof
US20060057651A1 (en) 2000-12-08 2006-03-16 Bowdish Katherine S Polypeptides and antibodies derived from chronic lymphocytic leukemia cells and uses thereof
EP1341902A2 (en) 2000-12-08 2003-09-10 Alexion Pharmaceuticals, Inc. Chronic lymphocytic leukemia cell line and its use for producing an antibody
US9249229B2 (en) 2000-12-08 2016-02-02 Alexion Pharmaceuticals, Inc. Polypeptides and antibodies derived from chronic lymphocytic leukemia cells and uses thereof
WO2002048401A2 (en) 2000-12-11 2002-06-20 Alexion Pharmaceuticals, Inc. Nested oligonucleotides containing hairpin for nucleic acid amplification
US7393656B2 (en) 2001-07-10 2008-07-01 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for risk stratification
US7414111B2 (en) * 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
US7593483B2 (en) * 2004-05-07 2009-09-22 Broadcom Corporation Nonlinear mapping in digital-to-analog and analog-to-digital converters
AU2005250432B2 (en) 2004-05-28 2011-09-15 Asuragen, Inc. Methods and compositions involving microRNA
US20060078894A1 (en) * 2004-10-12 2006-04-13 Winkler Matthew M Methods and compositions for analyzing nucleic acids
ES2534304T3 (en) 2004-11-12 2015-04-21 Asuragen, Inc. Procedures and compositions involving miRNA and miRNA inhibitor molecules
CA2621146A1 (en) * 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
RU2520088C2 (en) 2006-01-12 2014-06-20 Алексион Фармасьютикалз, Инк. Antibodies to ox-2/cd200 and their application
WO2007130967A2 (en) 2006-05-01 2007-11-15 Siemens Healthcare Diagnostics Inc. Novel oligonucleotide primers and methods for dna replication
WO2008036776A2 (en) * 2006-09-19 2008-03-27 Asuragen, Inc. Mir-15, mir-26, mir -31,mir -145, mir-147, mir-188, mir-215, mir-216 mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
AU2007333106A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. miR-20 regulated genes and pathways as targets for therapeutic intervention
WO2008073920A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-21 regulated genes and pathways as targets for therapeutic intervention
CN101622349A (en) * 2006-12-08 2010-01-06 奥斯瑞根公司 miR-20 regulated genes and pathways as targets for therapeutic intervention
DK2104737T3 (en) * 2006-12-08 2013-05-27 Asuragen Inc Functions and Purposes of Easy-7 Micro-RNAs
US20090175827A1 (en) * 2006-12-29 2009-07-09 Byrom Mike W miR-16 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
CN101801419A (en) * 2007-06-08 2010-08-11 米尔纳疗法公司 Gene and path as the miR-34 regulation and control for the treatment of the target of intervening
EP2185593B1 (en) 2007-07-25 2017-12-13 Alexion Pharmaceuticals, Inc. Compositions for treating autoimmune disease
US8361714B2 (en) 2007-09-14 2013-01-29 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
US20090186015A1 (en) * 2007-10-18 2009-07-23 Latham Gary J Micrornas differentially expressed in lung diseases and uses thereof
WO2009070805A2 (en) * 2007-12-01 2009-06-04 Asuragen, Inc. Mir-124 regulated genes and pathways as targets for therapeutic intervention
WO2009086156A2 (en) * 2007-12-21 2009-07-09 Asuragen, Inc. Mir-10 regulated genes and pathways as targets for therapeutic intervention
EP3699291A1 (en) 2008-01-17 2020-08-26 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
EP2260110B1 (en) * 2008-02-08 2014-11-12 Asuragen, INC. miRNAs DIFFERENTIALLY EXPRESSED IN LYMPH NODES FROM CANCER PATIENTS
US20090253780A1 (en) * 2008-03-26 2009-10-08 Fumitaka Takeshita COMPOSITIONS AND METHODS RELATED TO miR-16 AND THERAPY OF PROSTATE CANCER
US20090258928A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc. Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
EP2285960B1 (en) 2008-05-08 2015-07-08 Asuragen, INC. Compositions and methods related to mir-184 modulation of neovascularization or angiogenesis
AR072999A1 (en) * 2008-08-11 2010-10-06 Medarex Inc HUMAN ANTIBODIES THAT JOIN GEN 3 OF LYMPHOCYTARY ACTIVATION (LAG-3) AND THE USES OF THESE
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US8691510B2 (en) * 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
CN104195227B (en) * 2008-11-07 2017-04-12 适应生物技术公司 Methods of monitoring conditions by sequence analysis
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8748103B2 (en) * 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
WO2010056737A2 (en) * 2008-11-11 2010-05-20 Mirna Therapeutics, Inc. Methods and compositions involving mirnas in cancer stem cells
EP2387627B1 (en) 2009-01-15 2016-03-30 Adaptive Biotechnologies Corporation Adaptive immunity profiling and methods for generation of monoclonal antibodies
RU2539032C2 (en) 2009-06-25 2015-01-10 Фред Хатчинсон Кансэр Рисёч Сентер Method for measuring artificial immunity
US9043160B1 (en) 2009-11-09 2015-05-26 Sequenta, Inc. Method of determining clonotypes and clonotype profiles
JP6126991B2 (en) * 2010-09-27 2017-05-10 ヤンセン バイオテツク，インコーポレーテツド Antibody binding to human type II collagen
US8906649B2 (en) 2010-09-27 2014-12-09 Janssen Biotech, Inc. Antibodies binding human collagen II
CN103459598B (en) 2011-02-03 2016-08-10 米尔纳医疗股份有限公司 The synthesis analogies of MIR-124
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
AU2012325791B2 (en) 2011-10-21 2018-04-05 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
CA2858070C (en) 2011-12-09 2018-07-10 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
ES2662128T3 (en) 2012-03-05 2018-04-05 Adaptive Biotechnologies Corporation Determination of paired immune receptor chains from the frequency of matching subunits
ES2582554T3 (en) 2012-05-08 2016-09-13 Adaptive Biotechnologies Corporation Compositions and method for measuring and calibrating amplification bias in multiplexed PCR reactions
AR091649A1 (en) 2012-07-02 2015-02-18 Bristol Myers Squibb Co OPTIMIZATION OF ANTIBODIES THAT FIX THE LYMPHOCYTE ACTIVATION GEN 3 (LAG-3) AND ITS USES
CA2886647A1 (en) 2012-10-01 2014-04-10 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
EP2925878A1 (en) * 2012-11-28 2015-10-07 Abwiz Bio Inc. Preparation of gene-specific templates for the use in single primer amplification
GB2525568B (en) 2013-03-15 2020-10-14 Abvitro Llc Single cell barcoding for antibody discovery
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
SG11201601763SA (en) 2013-09-20 2016-04-28 Bristol Myers Squibb Co Combination of anti-lag-3 antibodies and anti-pd-1 antibodies to treat tumors
JOP20200094A1 (en) 2014-01-24 2017-06-16 Dana Farber Cancer Inst Inc Antibody molecules to pd-1 and uses thereof
CA2941612A1 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
KR102442436B1 (en) 2014-03-14 2022-09-15 노파르티스 아게 Antibody molecules to lag-3 and uses thereof
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
ES2777529T3 (en) 2014-04-17 2020-08-05 Adaptive Biotechnologies Corp Quantification of adaptive immune cell genomes in a complex mixture of cells
US10392663B2 (en) 2014-10-29 2019-08-27 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from a large number of samples
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
EP3224384A4 (en) 2014-11-25 2018-04-18 Adaptive Biotechnologies Corp. Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
ES2858306T3 (en) 2015-02-24 2021-09-30 Adaptive Biotechnologies Corp Method for determining HLA status by sequencing the immune repertoire
WO2016161273A1 (en) 2015-04-01 2016-10-06 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
LT3631454T (en) 2017-05-30 2023-11-27 Bristol-Myers Squibb Company Treatment of lag-3 positive tumors
AU2018275209A1 (en) 2017-05-30 2019-10-17 Bristol-Myers Squibb Company Compositions comprising an anti-LAG-3 antibody or an anti-LAG-3 antibody and an anti-PD-1 or anti-PD-L1 antibody
US11761963B2 (en) 2017-09-27 2023-09-19 Alexion Pharmaceuticals, Inc. Biomarker signature for predicting tumor response to anti-CD200 therapy
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5679512A (en) * 1992-07-31 1997-10-21 Behringwerke Ag Method for introducing defined sequences at the 3'end of polynucleotides

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR8108820A (en) 1980-09-24 1982-08-24 Cetus Corp DIAGNOSTIC PROCESS AND PROBE
US4582788A (en) * 1982-01-22 1986-04-15 Cetus Corporation HLA typing method and cDNA probes used therein
ATE53862T1 (en) 1982-01-22 1990-06-15 Cetus Corp METHODS FOR THE CHARACTERIZATION OF HLA AND THE CDNS TEST AGENTS USED THEREIN.
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5508178A (en) * 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
US6291159B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US6291160B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US6291158B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertoire
US6291161B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertiore
US5595891A (en) * 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
DK1471142T3 (en) * 1991-04-10 2009-03-09 Scripps Research Inst Heterodimeric receptor libraries using phagemids
FR2724934B1 (en) * 1994-09-26 1997-01-24 Bio Merieux CHIMERIC OLIGONUCLEOTIDE AND ITS USE IN OBTAINING NUCLEIC ACID TRANSCRIPTS
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
JP4236812B2 (en) 1997-09-12 2009-03-11 エクシコン エ／エス Oligonucleotide analogues
GB9817055D0 (en) * 1998-08-05 1998-09-30 Medical Res Council Reverse transcription and amplification processes and primers therefore
US6465182B1 (en) * 1999-04-29 2002-10-15 The Regents Of The University Of California Comparative fluorescence hybridization to oligonucleotide microarrays
WO2001006004A2 (en) 1999-07-19 2001-01-25 Cambridge University Technical Services Ltd. A method for amplifying low abundance nucleic acid sequences and means for performing said method
WO2001012855A2 (en) * 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags
DK1218542T3 (en) * 1999-09-13 2004-08-02 Nugen Technologies Inc Methods and compositions for linear isothermal amplification of polynucleotide sequences
US6618679B2 (en) 2000-01-28 2003-09-09 Althea Technologies, Inc. Methods for analysis of gene expression
WO2002048401A2 (en) * 2000-12-11 2002-06-20 Alexion Pharmaceuticals, Inc. Nested oligonucleotides containing hairpin for nucleic acid amplification

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5679512A (en) * 1992-07-31 1997-10-21 Behringwerke Ag Method for introducing defined sequences at the 3'end of polynucleotides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATEL ET AL.: "Formation of chimeric DNA primer extension products by template switching onto an annealed downstream oligonucleotide", PROC. NATL. ACAD. SCI. USA, vol. 93, April 1996 (1996-04-01), pages 2969 - 2974, XP002158856 *

Also Published As

Publication number Publication date
US7306906B2 (en) 2007-12-11
AU2002341752B2 (en) 2008-04-03
CA2460639C (en) 2013-07-16
ES2336433T3 (en) 2010-04-13
CY1109828T1 (en) 2014-09-10
JP2005503802A (en) 2005-02-10
PT1436404E (en) 2009-12-16
JP4439262B2 (en) 2010-03-24
EP1436404B1 (en) 2009-11-11
EP1436404A4 (en) 2006-07-05
DE60234369D1 (en) 2009-12-24
US20040072164A1 (en) 2004-04-15
EP1436404A2 (en) 2004-07-14
ATE448320T1 (en) 2009-11-15
WO2003025202A2 (en) 2003-03-27
DK1436404T3 (en) 2010-03-08
JP2009089716A (en) 2009-04-30
CA2460639A1 (en) 2003-03-27
JP5107274B2 (en) 2012-12-26

Similar Documents

Publication Publication Date Title
WO2003025202A3 (en) Engineered templates and their use in single primer amplification
WO2005060641A3 (en) Engineered templates and their use in single primer amplification
WO2003008624A3 (en) Nucleic acid amplification using nicking agents
EP1256630A3 (en) Selective polymerase chain reaction of DNA of which base sequence is completely unknown
WO2001020035A3 (en) Methods and compositions for linear isothermal amplification of polynucleotide sequences
EP1275715A4 (en) Method for amplifying nucleic acid by using double-stranded nucleic acid as template
WO2003097794A3 (en) Universal-tagged oligonucleotide primers and methods of use
EP1201768A3 (en) Amplification using modified primers
WO2004065582A3 (en) Amplification of dna in a hairpin structure, and applications
WO2002101022A3 (en) Method for rapid amplification of dna
WO2006071776A3 (en) Ligation-based rna amplification
EP1765417A4 (en) Immunostimulatory oligonucleotide multimers
EP1241268A3 (en) Method for amplifying RNA
WO2003020979A8 (en) Methods for preparing nucleic acid samples
WO2001064952A3 (en) Method for reducing artifacts in nucleic acid amplification
WO2003039523A3 (en) OLIGONUCLEOTIDES MODIFIED WITH NOVEL α-L-RNA ANALOGUES
JP2005503802A5 (en)
WO2003044216A3 (en) Multiplex oligonucleotide addition and target amplification
WO2004037988A3 (en) Methods for synthesizing complementary dna
IL131966A0 (en) An in vitro method for construction of a dna library
WO2003004659A3 (en) Recombination systems and a method for removing nucleic acid sequences from the genome of eukaryotic organisms
WO2004001062A3 (en) Multiplex nucleic acid reactions
WO2004022770A3 (en) Compositions and methods for synthesizing nucleic acids
WO2002018586A1 (en) Design and construction of transcription template for synthesizing cell-free protein, and dilution batch-type method of synthesizing cell-free wheat germ protein by using the same
WO2003040363A1 (en) Rice transposon genes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BY BZ CA CH CN CO CR CU CZ DE DM DZ EC EE ES FI GB GD GE GH HR HU ID IL IN IS JP KE KG KP KR LC LK LR LS LT LU LV MA MD MG MN MW MX MZ NO NZ OM PH PL PT RU SD SE SG SI SK SL TJ TM TN TR TZ UA UG US UZ VN YU ZA ZM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ UG ZM ZW AM AZ BY KG KZ RU TJ TM AT BE BG CH CY CZ DK EE ES FI FR GB GR IE IT LU MC PT SE SK TR BF BJ CF CG CI GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2002341752

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2002775901

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2460639

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2003529974

Country of ref document: JP

WWP Wipo information: published in national office

Ref document number: 2002775901

Country of ref document: EP