WO2003023400A3 - Method and apparatus for assay based on light diffraction - Google Patents

Method and apparatus for assay based on light diffraction Download PDF

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Publication number
WO2003023400A3
WO2003023400A3 PCT/CA2002/001395 CA0201395W WO03023400A3 WO 2003023400 A3 WO2003023400 A3 WO 2003023400A3 CA 0201395 W CA0201395 W CA 0201395W WO 03023400 A3 WO03023400 A3 WO 03023400A3
Authority
WO
WIPO (PCT)
Prior art keywords
diffraction
patterns
optic element
specific receptors
present
Prior art date
Application number
PCT/CA2002/001395
Other languages
French (fr)
Other versions
WO2003023400A2 (en
Inventor
M Cynthia Goh
Richard Loo
Jane B Goh
Richard Mcaloney
Original Assignee
Axela Biosensors Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axela Biosensors Inc filed Critical Axela Biosensors Inc
Priority to CA002460233A priority Critical patent/CA2460233A1/en
Priority to EP02759993A priority patent/EP1434994A2/en
Priority to JP2003527420A priority patent/JP4554205B2/en
Publication of WO2003023400A2 publication Critical patent/WO2003023400A2/en
Publication of WO2003023400A3 publication Critical patent/WO2003023400A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property

Abstract

The present invention relates to a method and apparatus for detecting analytes in a medium, and more particularly the present invention relates to an assay based on light diffraction which appears or changes upon the binding of analytes to their specific receptors laid out in patterns on a substrate, which has high sensitivity due to the appropriate choice of such patterns. The present invention is based on the principle that the pattern of recognition elements, which gives rise to the diffraction of the incident light in a diffraction-based assay, can be chosen in such a way so as to facilitate detection, and to enhance the signal to be detected compared to known gratings such as parallel straight lines. In one aspect the substrate itself has a surface topography designed to enhance the diffraction pattern signals. In another aspect the substrate is a diffractive optic element having the analyte-specific receptors affixed to the optic element. In another aspect the diffractive optic element is used as a master stamp for producing patterns of analyte-specific receptors which give the signal enhancements.
PCT/CA2002/001395 2001-09-13 2002-09-13 Method and apparatus for assay based on light diffraction WO2003023400A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002460233A CA2460233A1 (en) 2001-09-13 2002-09-13 Method and apparatus for assay based on light diffraction
EP02759993A EP1434994A2 (en) 2001-09-13 2002-09-13 Method and apparatus for assay based on light diffraction
JP2003527420A JP4554205B2 (en) 2001-09-13 2002-09-13 Method and apparatus for calibration based on diffraction of light

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31862401P 2001-09-13 2001-09-13
US60/318,624 2001-09-13

Publications (2)

Publication Number Publication Date
WO2003023400A2 WO2003023400A2 (en) 2003-03-20
WO2003023400A3 true WO2003023400A3 (en) 2004-03-11

Family

ID=23238937

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2002/001395 WO2003023400A2 (en) 2001-09-13 2002-09-13 Method and apparatus for assay based on light diffraction

Country Status (5)

Country Link
US (3) US7314749B2 (en)
EP (1) EP1434994A2 (en)
JP (2) JP4554205B2 (en)
CA (2) CA2460233A1 (en)
WO (1) WO2003023400A2 (en)

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Also Published As

Publication number Publication date
JP2009282046A (en) 2009-12-03
US7879596B2 (en) 2011-02-01
CA2743332A1 (en) 2003-03-20
US7314749B2 (en) 2008-01-01
US20030049693A1 (en) 2003-03-13
EP1434994A2 (en) 2004-07-07
WO2003023400A2 (en) 2003-03-20
CA2460233A1 (en) 2003-03-20
US20110111487A1 (en) 2011-05-12
JP2005502065A (en) 2005-01-20
US20080180692A1 (en) 2008-07-31
JP4554205B2 (en) 2010-09-29
US8283156B2 (en) 2012-10-09

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