WO2003023363A3 - Microcantilever apparatus and methods for detection of enzymes - Google Patents

Microcantilever apparatus and methods for detection of enzymes Download PDF

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Publication number
WO2003023363A3
WO2003023363A3 PCT/US2002/028920 US0228920W WO03023363A3 WO 2003023363 A3 WO2003023363 A3 WO 2003023363A3 US 0228920 W US0228920 W US 0228920W WO 03023363 A3 WO03023363 A3 WO 03023363A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzymes
detection
methods
microcantilever
microcantilever apparatus
Prior art date
Application number
PCT/US2002/028920
Other languages
French (fr)
Other versions
WO2003023363A2 (en
Inventor
Lawrence A Bottomley
Madhushree Ghosh
Shanxiang Shen
Richard Saul
Original Assignee
Protiveris Inc
Lawrence A Bottomley
Madhushree Ghosh
Shanxiang Shen
Richard Saul
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Protiveris Inc, Lawrence A Bottomley, Madhushree Ghosh, Shanxiang Shen, Richard Saul filed Critical Protiveris Inc
Priority to JP2003527389A priority Critical patent/JP2005502873A/en
Priority to CA002459462A priority patent/CA2459462A1/en
Priority to EP02768839A priority patent/EP1425548A4/en
Publication of WO2003023363A2 publication Critical patent/WO2003023363A2/en
Publication of WO2003023363A3 publication Critical patent/WO2003023363A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

An apparatus (Figure 1) and a method are provided for detecting an enzyme by measuring a change in defection of a microcantilever having a substrate for the enzyme.
PCT/US2002/028920 2001-09-12 2002-09-11 Microcantilever apparatus and methods for detection of enzymes WO2003023363A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2003527389A JP2005502873A (en) 2001-09-12 2002-09-11 Microcantilever apparatus and method for enzyme detection
CA002459462A CA2459462A1 (en) 2001-09-12 2002-09-11 Microcantilever apparatus and methods for detection of enzymes
EP02768839A EP1425548A4 (en) 2001-09-12 2002-09-11 Microcantilever apparatus and methods for detection of enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/951,131 US20030068655A1 (en) 2001-09-12 2001-09-12 Microcantilever apparatus and methods for detection of enzymes
US09/951,131 2001-09-12

Publications (2)

Publication Number Publication Date
WO2003023363A2 WO2003023363A2 (en) 2003-03-20
WO2003023363A3 true WO2003023363A3 (en) 2003-10-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/028920 WO2003023363A2 (en) 2001-09-12 2002-09-11 Microcantilever apparatus and methods for detection of enzymes

Country Status (5)

Country Link
US (2) US20030068655A1 (en)
EP (1) EP1425548A4 (en)
JP (1) JP2005502873A (en)
CA (1) CA2459462A1 (en)
WO (1) WO2003023363A2 (en)

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Also Published As

Publication number Publication date
US20030068655A1 (en) 2003-04-10
CA2459462A1 (en) 2003-03-20
JP2005502873A (en) 2005-01-27
WO2003023363A2 (en) 2003-03-20
EP1425548A2 (en) 2004-06-09
US20040029108A1 (en) 2004-02-12
EP1425548A4 (en) 2005-07-27
US7141385B2 (en) 2006-11-28

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