WO2003021230A3 - High-throughput electrophysiological measurement system - Google Patents

High-throughput electrophysiological measurement system Download PDF

Info

Publication number
WO2003021230A3
WO2003021230A3 PCT/US2002/028398 US0228398W WO03021230A3 WO 2003021230 A3 WO2003021230 A3 WO 2003021230A3 US 0228398 W US0228398 W US 0228398W WO 03021230 A3 WO03021230 A3 WO 03021230A3
Authority
WO
WIPO (PCT)
Prior art keywords
samples
measurement system
computer
methods
throughput
Prior art date
Application number
PCT/US2002/028398
Other languages
French (fr)
Other versions
WO2003021230A2 (en
Inventor
Kirk S Schroeder
Bradley D Neagle
Original Assignee
Essen Instr Inc
Kirk S Schroeder
Bradley D Neagle
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2002/016122 external-priority patent/WO2002095357A2/en
Application filed by Essen Instr Inc, Kirk S Schroeder, Bradley D Neagle filed Critical Essen Instr Inc
Priority to AU2002343338A priority Critical patent/AU2002343338A1/en
Priority to EP02780275A priority patent/EP1434850A2/en
Publication of WO2003021230A2 publication Critical patent/WO2003021230A2/en
Publication of WO2003021230A3 publication Critical patent/WO2003021230A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid

Abstract

Apparatus and methods are provided for performing electrophysiological measurements on membranous samples, including living cells, isolated cell fragments, such as organelles, and/or artificial membranes, such as vesicles. The apparatus may include a high-throughput electrophysiological measurement system, and components thereof. The measurement system may include a fluidics head (80) for transferring samples and/or other compounds to a perforated measurement substrate; pressure-regulated plenum system (72) for positioning samples on the substrate and subsequently forming a high-resistance electrical seal; activation system, such as a computer-controlled pulsed UV illumination module, for activating caged compounds; electronics head (84) for applying and/or measuring voltage and/or measuring voltage and/or current; and/or computer- controlled analysis system (96) for collecting and/or analyzing data. The methods are for performing high-throughput electrophysiological measurements on transporters and/or voltage or ligand-gated ion channels sequentially and/or simultaneously.
PCT/US2002/028398 2001-09-05 2002-09-05 High-throughput electrophysiological measurement system WO2003021230A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002343338A AU2002343338A1 (en) 2001-09-05 2002-09-05 High-throughput electrophysiological measurement system
EP02780275A EP1434850A2 (en) 2001-09-05 2002-09-05 High-throughput electrophysiological measurement system

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US31711201P 2001-09-05 2001-09-05
US60/317,112 2001-09-05
USPCT/US02/16122 2002-05-21
PCT/US2002/016122 WO2002095357A2 (en) 2001-05-21 2002-05-21 Chemical activation in electrophysiological measurements
US38319602P 2002-05-22 2002-05-22
US60/383,196 2002-05-22

Publications (2)

Publication Number Publication Date
WO2003021230A2 WO2003021230A2 (en) 2003-03-13
WO2003021230A3 true WO2003021230A3 (en) 2003-10-30

Family

ID=27359127

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/028398 WO2003021230A2 (en) 2001-09-05 2002-09-05 High-throughput electrophysiological measurement system

Country Status (2)

Country Link
EP (1) EP1434850A2 (en)
WO (1) WO2003021230A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7514046B2 (en) 2000-10-31 2009-04-07 Caliper Life Sciences, Inc. Methods and systems for processing microscale devices for reuse
US20040181343A1 (en) * 2002-11-01 2004-09-16 Cellectricon Ab Computer program products and systems for rapidly changing the solution environment around sensors
US20050014288A1 (en) * 2003-07-15 2005-01-20 Amphora Discovery Corporation Motion induced electrical field stimulation
CA2516340A1 (en) * 2003-08-04 2005-02-24 Caliper Life Sciences, Inc. Methods and systems for processing microscale devices for reuse
SE0400421D0 (en) * 2004-02-23 2004-02-23 Astrazeneca Ab assay
US9080999B2 (en) * 2011-09-02 2015-07-14 Molecular Devices, Llc Voltage offset correction in high-throughput electrophysiological measurement system
CN108624496B (en) * 2017-03-23 2022-01-25 洛阳华清天木生物科技有限公司 Sample conveying and collecting device for microorganism mutation breeding instrument

Citations (15)

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US4071315A (en) * 1976-06-02 1978-01-31 Guy Chateau Analytical tape medium
US4952518A (en) * 1984-10-01 1990-08-28 Cetus Corporation Automated assay machine and assay tray
US5169600A (en) * 1987-07-15 1992-12-08 Fuji Photo Film Co., Ltd. Biochemical analysis apparatus for incubating and analyzing test sites on a long tape test film
US5262128A (en) * 1989-10-23 1993-11-16 The United States Of America As Represented By The Department Of Health And Human Services Array-type multiple cell injector
US5508200A (en) * 1992-10-19 1996-04-16 Tiffany; Thomas Method and apparatus for conducting multiple chemical assays
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US6063260A (en) * 1994-10-28 2000-05-16 Neurosearch A/S Patch clamp apparatus and technique having high throughput and low fluid volume requirements
US6132582A (en) * 1998-09-14 2000-10-17 The Perkin-Elmer Corporation Sample handling system for a multi-channel capillary electrophoresis device
US20010005774A1 (en) * 1999-12-24 2001-06-28 Hirokazu Kato Automatic electrophysiological measuring apparatus / method
US20010005489A1 (en) * 1998-07-02 2001-06-28 Roach David J. Apparatus and method for filling and cleaning channels and inlet ports in microchips used for biological analysis
US6368851B1 (en) * 1998-05-27 2002-04-09 Micronas Gmbh Method and apparatus for measuring a state variable
US6377057B1 (en) * 1999-02-18 2002-04-23 The Board Of Trustees Of The Leland Stanford Junior University Classification of biological agents according to the spectral density signature of evoked changes in cellular electric potential
US6461860B2 (en) * 2001-01-25 2002-10-08 Axon Instruments, Inc. Alignment mechanism for two-electrode voltage-clamp perfusion chamber for electrophysiological testing of oocytes
US6470226B1 (en) * 1997-05-01 2002-10-22 Sophion Bioscience A/S Automatic electrode positioning apparatus
US6488829B1 (en) * 1999-08-05 2002-12-03 Essen Instruments Inc High-throughput electrophysiological measurement apparatus

Patent Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4071315A (en) * 1976-06-02 1978-01-31 Guy Chateau Analytical tape medium
US4952518A (en) * 1984-10-01 1990-08-28 Cetus Corporation Automated assay machine and assay tray
US5169600A (en) * 1987-07-15 1992-12-08 Fuji Photo Film Co., Ltd. Biochemical analysis apparatus for incubating and analyzing test sites on a long tape test film
US5262128A (en) * 1989-10-23 1993-11-16 The United States Of America As Represented By The Department Of Health And Human Services Array-type multiple cell injector
US5508200A (en) * 1992-10-19 1996-04-16 Tiffany; Thomas Method and apparatus for conducting multiple chemical assays
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US6063260A (en) * 1994-10-28 2000-05-16 Neurosearch A/S Patch clamp apparatus and technique having high throughput and low fluid volume requirements
US6470226B1 (en) * 1997-05-01 2002-10-22 Sophion Bioscience A/S Automatic electrode positioning apparatus
US6368851B1 (en) * 1998-05-27 2002-04-09 Micronas Gmbh Method and apparatus for measuring a state variable
US20010005489A1 (en) * 1998-07-02 2001-06-28 Roach David J. Apparatus and method for filling and cleaning channels and inlet ports in microchips used for biological analysis
US6132582A (en) * 1998-09-14 2000-10-17 The Perkin-Elmer Corporation Sample handling system for a multi-channel capillary electrophoresis device
US6377057B1 (en) * 1999-02-18 2002-04-23 The Board Of Trustees Of The Leland Stanford Junior University Classification of biological agents according to the spectral density signature of evoked changes in cellular electric potential
US6488829B1 (en) * 1999-08-05 2002-12-03 Essen Instruments Inc High-throughput electrophysiological measurement apparatus
US20010005774A1 (en) * 1999-12-24 2001-06-28 Hirokazu Kato Automatic electrophysiological measuring apparatus / method
US6461860B2 (en) * 2001-01-25 2002-10-08 Axon Instruments, Inc. Alignment mechanism for two-electrode voltage-clamp perfusion chamber for electrophysiological testing of oocytes

Also Published As

Publication number Publication date
EP1434850A2 (en) 2004-07-07
WO2003021230A2 (en) 2003-03-13

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