WO2003019137A3 - Methods for screening antibody-producing cells on heterogenous antigen substrates - Google Patents

Methods for screening antibody-producing cells on heterogenous antigen substrates Download PDF

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Publication number
WO2003019137A3
WO2003019137A3 PCT/US2002/026529 US0226529W WO03019137A3 WO 2003019137 A3 WO2003019137 A3 WO 2003019137A3 US 0226529 W US0226529 W US 0226529W WO 03019137 A3 WO03019137 A3 WO 03019137A3
Authority
WO
WIPO (PCT)
Prior art keywords
antigens
methods
producing cells
binding
screening antibody
Prior art date
Application number
PCT/US2002/026529
Other languages
French (fr)
Other versions
WO2003019137A2 (en
Inventor
Steven Kessler
Original Assignee
Steven Kessler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Steven Kessler filed Critical Steven Kessler
Priority to AU2002332595A priority Critical patent/AU2002332595A1/en
Publication of WO2003019137A2 publication Critical patent/WO2003019137A2/en
Publication of WO2003019137A3 publication Critical patent/WO2003019137A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3069—Reproductive system, e.g. ovaria, uterus, testes, prostate

Abstract

Methods and compounds are disclosed that relate to screening and selection of monoclonal antibodies specific for antigens in heterogeneous antigen mixtures. Antibody-secreting cells such as hybridomas are modified to make them capable of directly binding antigens by capturing their secreted antibody products onto their surface membranes in appropriate binding density and orientation. Selectivity of binding to novel or desired antigens is achieved by first reacting the antigen mixtures affixed to a solid substrate with a polyclonal antibody library that prevents access to the majority of antigens or epitopes other than those that are novel or desired.
PCT/US2002/026529 2001-08-22 2002-08-21 Methods for screening antibody-producing cells on heterogenous antigen substrates WO2003019137A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002332595A AU2002332595A1 (en) 2001-08-22 2002-08-21 Methods for screening antibody-producing cells on heterogenous antigen substrates

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31407001P 2001-08-22 2001-08-22
US60/314,070 2001-08-22

Publications (2)

Publication Number Publication Date
WO2003019137A2 WO2003019137A2 (en) 2003-03-06
WO2003019137A3 true WO2003019137A3 (en) 2003-11-27

Family

ID=23218424

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/026529 WO2003019137A2 (en) 2001-08-22 2002-08-21 Methods for screening antibody-producing cells on heterogenous antigen substrates

Country Status (2)

Country Link
AU (1) AU2002332595A1 (en)
WO (1) WO2003019137A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2573313A1 (en) * 2004-07-10 2006-02-16 Alexion Pharmaceuticals, Inc. Antibodies against cancer produced using masked cancer cells as immunogen
EP2708890B1 (en) 2012-09-14 2015-11-18 Molecular Devices (New Milton) Ltd Method of selecting a monoclonal cell colony

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6250050B1 (en) * 2000-03-13 2001-06-26 North American Container Corp. Wing-end wood-cleated corrugated paperboard container and method
US6335163B1 (en) * 1994-01-31 2002-01-01 The Trustees Of Boston University Polyclonal antibody libraries

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335163B1 (en) * 1994-01-31 2002-01-01 The Trustees Of Boston University Polyclonal antibody libraries
US6250050B1 (en) * 2000-03-13 2001-06-26 North American Container Corp. Wing-end wood-cleated corrugated paperboard container and method

Also Published As

Publication number Publication date
AU2002332595A1 (en) 2003-03-10
WO2003019137A2 (en) 2003-03-06

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