WO2003012389A3 - Method for analysing macromolecules, analysis device and a method for producing an analysis device - Google Patents

Method for analysing macromolecules, analysis device and a method for producing an analysis device Download PDF

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Publication number
WO2003012389A3
WO2003012389A3 PCT/EP2002/007644 EP0207644W WO03012389A3 WO 2003012389 A3 WO2003012389 A3 WO 2003012389A3 EP 0207644 W EP0207644 W EP 0207644W WO 03012389 A3 WO03012389 A3 WO 03012389A3
Authority
WO
WIPO (PCT)
Prior art keywords
drop
macromolecules
liquid
spots
microarray
Prior art date
Application number
PCT/EP2002/007644
Other languages
German (de)
French (fr)
Other versions
WO2003012389A2 (en
Inventor
Juergen Scriba
Christoph Gauer
Original Assignee
Advalytix Ag
Juergen Scriba
Christoph Gauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advalytix Ag, Juergen Scriba, Christoph Gauer filed Critical Advalytix Ag
Priority to AU2002328867A priority Critical patent/AU2002328867A1/en
Priority to EP02764659A priority patent/EP1412533A2/en
Publication of WO2003012389A2 publication Critical patent/WO2003012389A2/en
Publication of WO2003012389A3 publication Critical patent/WO2003012389A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00533—Sheets essentially rectangular
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0436—Moving fluids with specific forces or mechanical means specific forces vibrational forces acoustic forces, e.g. surface acoustic waves [SAW]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0493—Specific techniques used
    • B01L2400/0496—Travelling waves, e.g. in combination with electrical or acoustic forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

The invention relates to a method for analysing macromolecules using a microarray, which consists of several spots in a known arrangement. Said spots comprise a plurality of first macromolecules that differ at least partially. The microarray is located on the surface of a solid body, preferably a chip. According to the method, at least one drop of liquid containing macromolecules is applied to the surface of the solid body, the quantity of liquid being so small that only one spot or a small group of spots of the microarray comes or come into contact with the drop of liquid. After a predetermined time has elapsed, the drop of liquid is displaced onto a subsequent spot or an additional small group of spots with the help of the transmission pulse of a surface sound wave. The spots that come into contact with the drop of liquid are then analysed to identify whether a reaction with the material of said drop has occurred. The invention also relates to a method for producing an analysis device for macromolecules. According to said method, at least one drop of liquid containing at least one type of macromolecule is applied to the surface of a solid body, preferably a chip, whereby the macromolecules have an active group that is activated during a modification of a corresponding external parameter, in order to allow bonding to the surface or to molecules that have already been bonded. The drop is displaced to a desired location on the surface with the help of the transmission pulse of one or more surface sound waves. The external parameter is modified in a homogeneous, non-local manner, permitting the bonding of the macromolecules to form the spot of a microarray, in which macromolecules from the drop of liquid are bonded to the surface. The invention also relates to an analysis device for carrying out the inventive analysis method.
PCT/EP2002/007644 2001-07-24 2002-07-09 Method for analysing macromolecules, analysis device and a method for producing an analysis device WO2003012389A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002328867A AU2002328867A1 (en) 2001-07-24 2002-07-09 Method for analysing macromolecules, analysis device and a method for producing an analysis device
EP02764659A EP1412533A2 (en) 2001-07-24 2002-07-09 Method for analysing macromolecules, analysis device and a method for producing an analysis device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10136008.8 2001-07-24
DE10136008A DE10136008B4 (en) 2001-07-24 2001-07-24 Method for analyzing macromolecules and method for producing an analysis device

Publications (2)

Publication Number Publication Date
WO2003012389A2 WO2003012389A2 (en) 2003-02-13
WO2003012389A3 true WO2003012389A3 (en) 2003-07-17

Family

ID=7692898

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/007644 WO2003012389A2 (en) 2001-07-24 2002-07-09 Method for analysing macromolecules, analysis device and a method for producing an analysis device

Country Status (4)

Country Link
EP (1) EP1412533A2 (en)
AU (1) AU2002328867A1 (en)
DE (1) DE10136008B4 (en)
WO (1) WO2003012389A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10328624A1 (en) * 2003-06-25 2005-01-20 Advalytix Ag A hybridizing process, for the study of biological material, the spots of an array on a microscope slide are in contact with a hybridizing solution in a set height level for the reaction time
DE102004037348A1 (en) * 2004-08-02 2006-03-16 Infineon Technologies Ag Fluid transport device, sensor assembly, fluid mixing device and method of manufacturing a fluid transport device
DE102004051394B4 (en) * 2004-10-21 2006-08-17 Advalytix Ag Method for moving small amounts of liquid in microchannels and microchannel system
EP1833598B1 (en) 2005-01-05 2008-10-08 Olympus Life Science Research Europa GmbH Method and device for dosing and mixing small amounts of liquid
DE102005000835B3 (en) 2005-01-05 2006-09-07 Advalytix Ag Method and device for dosing small quantities of liquid
DE102005056639A1 (en) * 2005-11-28 2007-06-06 Advalytix Ag Method, device and kit for the study of macromolecules in a sample
DE102006056694B4 (en) * 2006-11-30 2010-08-05 Advalytix Ag Method for carrying out an enzymatic reaction
WO2008080531A2 (en) * 2007-01-05 2008-07-10 Advalytix Ag Method, device, and kit for analyzing a liquid sample
EP2194381B1 (en) * 2008-12-03 2015-12-02 Roche Diagnostics GmbH Testing element with combined control and calibration zone

Citations (4)

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WO1994027719A1 (en) * 1993-05-27 1994-12-08 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US5674742A (en) * 1992-08-31 1997-10-07 The Regents Of The University Of California Microfabricated reactor
US6010316A (en) * 1996-01-16 2000-01-04 The Board Of Trustees Of The Leland Stanford Junior University Acoustic micropump
DE19836110A1 (en) * 1998-08-10 2000-02-24 Biotul Bio Instr Gmbh Mixer effective close to liquid-biosensor interface employs parallel or transverse acoustic wave propagation between optical measurements, to agitate under controlled conditions of frequency and power, avoiding disruption

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US5846708A (en) * 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
JPH0927590A (en) * 1995-07-10 1997-01-28 Nippon Inter Electronics Corp Composite semiconductor device
DE19827900A1 (en) * 1997-10-21 1999-04-22 Roche Diagnostics Gmbh Process for coating surfaces
WO2000013018A2 (en) * 1998-08-28 2000-03-09 Febit Ferrarius Biotechnology Gmbh Support for a method for determining analytes and a method for producing the support
FR2783179B1 (en) * 1998-09-16 2000-10-06 Commissariat Energie Atomique CHEMICAL OR BIOLOGICAL ANALYSIS DEVICE COMPRISING A PLURALITY OF ANALYSIS SITES ON A MEDIUM, AND ITS MANUFACTURING METHOD

Patent Citations (4)

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US5674742A (en) * 1992-08-31 1997-10-07 The Regents Of The University Of California Microfabricated reactor
WO1994027719A1 (en) * 1993-05-27 1994-12-08 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US6010316A (en) * 1996-01-16 2000-01-04 The Board Of Trustees Of The Leland Stanford Junior University Acoustic micropump
DE19836110A1 (en) * 1998-08-10 2000-02-24 Biotul Bio Instr Gmbh Mixer effective close to liquid-biosensor interface employs parallel or transverse acoustic wave propagation between optical measurements, to agitate under controlled conditions of frequency and power, avoiding disruption

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Also Published As

Publication number Publication date
DE10136008B4 (en) 2005-03-31
EP1412533A2 (en) 2004-04-28
WO2003012389A2 (en) 2003-02-13
AU2002328867A1 (en) 2003-02-17
DE10136008A1 (en) 2003-02-20

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