WO2002097109A3 - Compositions and methods for extension of nucleic acids - Google Patents

Compositions and methods for extension of nucleic acids Download PDF

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Publication number
WO2002097109A3
WO2002097109A3 PCT/US2002/016063 US0216063W WO02097109A3 WO 2002097109 A3 WO2002097109 A3 WO 2002097109A3 US 0216063 W US0216063 W US 0216063W WO 02097109 A3 WO02097109 A3 WO 02097109A3
Authority
WO
WIPO (PCT)
Prior art keywords
stranded
compositions
methods
polypeptides
nucleic acid
Prior art date
Application number
PCT/US2002/016063
Other languages
French (fr)
Other versions
WO2002097109A2 (en
Inventor
Thomas F Kort
Mekbib Astatke
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Priority to AU2002303827A priority Critical patent/AU2002303827A1/en
Publication of WO2002097109A2 publication Critical patent/WO2002097109A2/en
Publication of WO2002097109A3 publication Critical patent/WO2002097109A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

The present invention relates to polypeptides, compositions and methods for extending nucleic acid molecules. Specifically, the present invention discloses polypeptides able to extend double-stranded and/or single-stranded nucleic acid molecules and/or single-stranded/double-stranded nucleic acid complexes (e.g., primer/template complexes, double-stranded templates, single-stranded templates or single-stranded primers) and more particularly when a mismatched dimer is present at or near the 3' terminus of the primer. Accordingly, the polypeptide of the invention extends nucleic acids in a non-templated fashion. The invention also relates to kits for synthesizing and extending nucleic acid molecules comprising one or more of the polypeptides or compositions of the invention. The invention also relates to compositions prepared for carying out the methods of the invention and to compositions made after or during such methods. The invention also generally relates to polypeptides and compositions useful for detecting and determining the site and charasteristic of single polynucleotide polymorphisms.
PCT/US2002/016063 2001-05-25 2002-05-23 Compositions and methods for extension of nucleic acids WO2002097109A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002303827A AU2002303827A1 (en) 2001-05-25 2002-05-23 Compositions and methods for extension of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29318201P 2001-05-25 2001-05-25
US60/293,182 2001-05-25

Publications (2)

Publication Number Publication Date
WO2002097109A2 WO2002097109A2 (en) 2002-12-05
WO2002097109A3 true WO2002097109A3 (en) 2003-05-08

Family

ID=23128025

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/016063 WO2002097109A2 (en) 2001-05-25 2002-05-23 Compositions and methods for extension of nucleic acids

Country Status (3)

Country Link
US (1) US20030129607A1 (en)
AU (1) AU2002303827A1 (en)
WO (1) WO2002097109A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008070370A2 (en) 2006-11-02 2008-06-12 University Of Utah Research Foundation Oligonucleotides for use in allele-specific pcr

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5037756A (en) * 1986-04-21 1991-08-06 The United States Of America As Represented By The Secretary Of The Army Recombinant DNA molecules for producing terminal transferase-like polypeptides
US5871963A (en) * 1995-11-15 1999-02-16 Cor Therapeutics, Inc. P2u2 purinergic receptor and nucleic acid encoding the receptor
US6235480B1 (en) * 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids

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GB8311018D0 (en) * 1983-04-22 1983-05-25 Amersham Int Plc Detecting mutations in dna
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
GB8606719D0 (en) * 1986-03-19 1986-04-23 Lister Preventive Med Genetic probes
US4962022A (en) * 1986-09-22 1990-10-09 Becton Dickinson And Company Storage and use of liposomes
US5498523A (en) * 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
US5075217A (en) * 1989-04-21 1991-12-24 Marshfield Clinic Length polymorphisms in (dC-dA)n ·(dG-dT)n sequences
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5800992A (en) * 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5252743A (en) * 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5318961A (en) * 1990-08-01 1994-06-07 Merrell Dow Pharmaceuticals Inc. 4-amino-Δ4-steroids and their use as 5α-reductase inhibitors
US5888819A (en) * 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
US6004744A (en) * 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5762876A (en) * 1991-03-05 1998-06-09 Molecular Tool, Inc. Automatic genotype determination
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US5763170A (en) * 1991-04-16 1998-06-09 Amersham International Plc Method for forming an array of biological particles
US5412087A (en) * 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
WO1993014217A1 (en) * 1992-01-10 1993-07-22 Life Technologies, Inc. Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
US5837832A (en) * 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5599695A (en) * 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
GB9604305D0 (en) * 1996-02-29 1996-05-01 Ralston Stuart H Diagnostic method and apparatus
GB9608133D0 (en) * 1996-04-19 1996-06-26 Gemini International Holdings Diagnostic method and apparatus
GB9610281D0 (en) * 1996-05-16 1996-07-24 Ralston Stuart H Diagnostic method and apparatus
US6291164B1 (en) * 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5037756A (en) * 1986-04-21 1991-08-06 The United States Of America As Represented By The Secretary Of The Army Recombinant DNA molecules for producing terminal transferase-like polypeptides
US5871963A (en) * 1995-11-15 1999-02-16 Cor Therapeutics, Inc. P2u2 purinergic receptor and nucleic acid encoding the receptor
US6235480B1 (en) * 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids

Also Published As

Publication number Publication date
AU2002303827A1 (en) 2002-12-09
US20030129607A1 (en) 2003-07-10
WO2002097109A2 (en) 2002-12-05

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