WO2002084271A3 - Methods and sensors for luminescent and optoelectronic detection and analysis of polynucleotides - Google Patents

Methods and sensors for luminescent and optoelectronic detection and analysis of polynucleotides Download PDF

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Publication number
WO2002084271A3
WO2002084271A3 PCT/US2002/012176 US0212176W WO02084271A3 WO 2002084271 A3 WO2002084271 A3 WO 2002084271A3 US 0212176 W US0212176 W US 0212176W WO 02084271 A3 WO02084271 A3 WO 02084271A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
polynucleotide
sensor
sensor polynucleotide
polynucleotides
Prior art date
Application number
PCT/US2002/012176
Other languages
French (fr)
Other versions
WO2002084271A2 (en
Inventor
Daniel E Morse
Galen D Stucky
Jennifer N Cha
Original Assignee
Univ California
Daniel E Morse
Galen D Stucky
Jennifer N Cha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Daniel E Morse, Galen D Stucky, Jennifer N Cha filed Critical Univ California
Publication of WO2002084271A2 publication Critical patent/WO2002084271A2/en
Publication of WO2002084271A3 publication Critical patent/WO2002084271A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

Abstract

Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a single-stranded sensor polynucleotide complementary to the target polynucleotide and an agent that allows the sensor polynucleotide itself, when present in single-stranded form, to fluoresce upon excitation. The sensor polynucleotide is optionally conjugated to a substrate, which may be an optoelectronic sensing device, and can be micro- or nanoaddressable. A chromophore may be used to adsorb energy from the excited sensor polynucleotide and emit light. The methods can be used in multiplex form. Sensing devices incorporating the sensor polynucleotide and optionally the chromophore are also provided. Kits comprising reagents for performing such methods are also provided.
PCT/US2002/012176 2001-04-16 2002-04-16 Methods and sensors for luminescent and optoelectronic detection and analysis of polynucleotides WO2002084271A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83657901A 2001-04-16 2001-04-16
US09/836,579 2001-04-16

Publications (2)

Publication Number Publication Date
WO2002084271A2 WO2002084271A2 (en) 2002-10-24
WO2002084271A3 true WO2002084271A3 (en) 2002-12-12

Family

ID=25272267

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/012176 WO2002084271A2 (en) 2001-04-16 2002-04-16 Methods and sensors for luminescent and optoelectronic detection and analysis of polynucleotides

Country Status (1)

Country Link
WO (1) WO2002084271A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7914984B2 (en) 2003-09-17 2011-03-29 The Regents Of The University Of California Conformationally flexible cationic conjugated polymers

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10001475B2 (en) 2002-06-20 2018-06-19 The Regents Of The University Of California Light harvesting multichromophore compositions and methods of using the same
US9371559B2 (en) 2002-06-20 2016-06-21 The Regents Of The University Of California Compositions for detection and analysis of polynucleotides using light harvesting multichromophores
EP1549772B1 (en) * 2002-08-26 2013-01-02 The Regents of The University of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
WO2004092324A2 (en) 2002-08-26 2004-10-28 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
JP4740111B2 (en) 2003-02-13 2011-08-03 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Methods and compositions for detecting and analyzing polynucleotide binding protein interactions using light collecting multichromophores
WO2006074482A2 (en) 2005-01-10 2006-07-13 The Regents Of The University Of California Methods and kits for strand-specific polynucleotide detection with cationic multichromophores
EP1838752B1 (en) 2005-01-10 2017-10-04 The Regents of The University of California Cationic conjugated polymers suitable for strand-specific polynucleotide detection in homogeneous and solid state assays
WO2006083932A2 (en) 2005-01-31 2006-08-10 The Regents Of The University Methods and compositions for aggregant detection

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4840892A (en) * 1984-05-15 1989-06-20 Smithkline Beckman Corporation Polynucleotide hybridization probes
US5851772A (en) * 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
US5998597A (en) * 1996-10-30 1999-12-07 Photogen, Inc. Method for improved selectivity in photo-activation of molecular agents
US6015880A (en) * 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
US6054277A (en) * 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US6110339A (en) * 1995-06-08 2000-08-29 Visible Genetics Inc. Nanofabricated separation matrix for analysis of biopolymers and methods of making and using same
US6258606B1 (en) * 1996-07-09 2001-07-10 Nanogen, Inc. Multiplexed active biologic array
US6262241B1 (en) * 1990-08-13 2001-07-17 Isis Pharmaceuticals, Inc. Compound for detecting and modulating RNA activity and gene expression
US6274323B1 (en) * 1999-05-07 2001-08-14 Quantum Dot Corporation Method of detecting an analyte in a sample using semiconductor nanocrystals as a detectable label

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4840892A (en) * 1984-05-15 1989-06-20 Smithkline Beckman Corporation Polynucleotide hybridization probes
US6262241B1 (en) * 1990-08-13 2001-07-17 Isis Pharmaceuticals, Inc. Compound for detecting and modulating RNA activity and gene expression
US6015880A (en) * 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
US6110339A (en) * 1995-06-08 2000-08-29 Visible Genetics Inc. Nanofabricated separation matrix for analysis of biopolymers and methods of making and using same
US5851772A (en) * 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
US6054277A (en) * 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US6258606B1 (en) * 1996-07-09 2001-07-10 Nanogen, Inc. Multiplexed active biologic array
US5998597A (en) * 1996-10-30 1999-12-07 Photogen, Inc. Method for improved selectivity in photo-activation of molecular agents
US6274323B1 (en) * 1999-05-07 2001-08-14 Quantum Dot Corporation Method of detecting an analyte in a sample using semiconductor nanocrystals as a detectable label

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MORRISON L.E. ET AL.: "Solution-phase detection of polynucleotides using interacting fluorescent labels and competitive hybridization", ANALYTICAL BIOCHEMISTRY, vol. 183, June 1989 (1989-06-01), pages 231 - 244, XP002113097 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7914984B2 (en) 2003-09-17 2011-03-29 The Regents Of The University Of California Conformationally flexible cationic conjugated polymers

Also Published As

Publication number Publication date
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