WO2002081738A3 - System and method for detecting and quantifying nucleic acids using covalent binding - Google Patents
System and method for detecting and quantifying nucleic acids using covalent binding Download PDFInfo
- Publication number
- WO2002081738A3 WO2002081738A3 PCT/EP2002/003891 EP0203891W WO02081738A3 WO 2002081738 A3 WO2002081738 A3 WO 2002081738A3 EP 0203891 W EP0203891 W EP 0203891W WO 02081738 A3 WO02081738 A3 WO 02081738A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acids
- detector
- target nucleic
- detector reagents
- oligonucleotides
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Abstract
Target nucleic acids bound to immobilized capture molecules by molecular biological or immunochemical reactions are detected with reactive residue-containing detector oligonucleotides and quantified with detector reagents capable of forming covalent linkages with the reactive residues on detector oligonucleotides. Alternatively, amplicons of target nucleic acids generated with reactive residue-containing primer oligonucleotides are bound to immobilized capture molecules by molecular biological or immunochemical reactions and quantified with detector reagents capable of forming covalent linkages with the reactive residues of captured amplicons. The quantity of reporter molecules provided or generated by covalently coupled detector reagents is a proportional measure of the quantity of target nucleic acids in the specimen. Enhanced covalent amplification systems include additional carrier systems for covalent attachment of multiple detector reagents and/or preformed covalently linked complexes of detector reagents.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02727542A EP1385997A2 (en) | 2001-04-09 | 2002-04-08 | System and method for detecting and quantifying nucleic acids using covalent binding |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28216901P | 2001-04-09 | 2001-04-09 | |
| US60/282,169 | 2001-04-09 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2002081738A2 WO2002081738A2 (en) | 2002-10-17 |
| WO2002081738A3 true WO2002081738A3 (en) | 2003-11-20 |
Family
ID=23080375
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP2002/003891 WO2002081738A2 (en) | 2001-04-09 | 2002-04-08 | System and method for detecting and quantifying nucleic acids using covalent binding |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP1385997A2 (en) |
| WO (1) | WO2002081738A2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ543336A (en) * | 2003-04-07 | 2009-01-31 | Layerlab Aktiebolag | Surface immobilised multilayer structure of vesicles |
| US8741577B2 (en) * | 2003-04-07 | 2014-06-03 | Bio-Rad Laboratories Inc. | Surface immobilised multilayer structure of vesicles |
| DK1718660T3 (en) | 2004-02-26 | 2011-03-21 | Layerlab Aktiebolag | Oligonucleotides associated with lipid membrane bonds |
| CN111413332B (en) * | 2020-04-09 | 2021-07-23 | 吉林大学 | Saccharide distinguishing method based on natural pigment anthocyanin |
| CN114849779B (en) * | 2022-04-02 | 2023-06-20 | 湖北大学 | Metal ligand-containing polymer catalyst and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0357011A2 (en) * | 1988-08-30 | 1990-03-07 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
| US5424188A (en) * | 1985-12-13 | 1995-06-13 | The Trustees Of Princeton University | Amplified hybridization assay |
| EP0670329A2 (en) * | 1988-09-26 | 1995-09-06 | Siska Diagnostics,Inc. | Enzyme-nucleic acid hydrazone and hydrazine conjugate compositions and methods |
| US5474895A (en) * | 1990-11-14 | 1995-12-12 | Siska Diagnostics Inc. | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
-
2002
- 2002-04-08 EP EP02727542A patent/EP1385997A2/en not_active Withdrawn
- 2002-04-08 WO PCT/EP2002/003891 patent/WO2002081738A2/en not_active Application Discontinuation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5424188A (en) * | 1985-12-13 | 1995-06-13 | The Trustees Of Princeton University | Amplified hybridization assay |
| EP0357011A2 (en) * | 1988-08-30 | 1990-03-07 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
| EP0670329A2 (en) * | 1988-09-26 | 1995-09-06 | Siska Diagnostics,Inc. | Enzyme-nucleic acid hydrazone and hydrazine conjugate compositions and methods |
| US5474895A (en) * | 1990-11-14 | 1995-12-12 | Siska Diagnostics Inc. | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
Non-Patent Citations (1)
| Title |
|---|
| DELAIR T ET AL: "CONJUGATION OF NUCLEIC ACID PROBES TO 6-AMINOGLUCOSE-BASED HOMO- AND COPOLYMERS. II. APPLICATION TO DIAGNOSTICS", POLYMERS FOR ADVANCED TECHNOLOGIES, JOHN WILEY AND SONS, CHICHESTER, GB, vol. 8, no. 9, 1 September 1997 (1997-09-01), pages 545 - 555, XP000696595, ISSN: 1042-7147 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002081738A2 (en) | 2002-10-17 |
| EP1385997A2 (en) | 2004-02-04 |
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