WO2002077271A3 - Control plasmid and method for detecting mycoplasma contamination in biological material - Google Patents

Control plasmid and method for detecting mycoplasma contamination in biological material Download PDF

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Publication number
WO2002077271A3
WO2002077271A3 PCT/DE2002/001154 DE0201154W WO02077271A3 WO 2002077271 A3 WO2002077271 A3 WO 2002077271A3 DE 0201154 W DE0201154 W DE 0201154W WO 02077271 A3 WO02077271 A3 WO 02077271A3
Authority
WO
WIPO (PCT)
Prior art keywords
mycoplasma
biological material
segment
control plasmid
primers
Prior art date
Application number
PCT/DE2002/001154
Other languages
German (de)
French (fr)
Other versions
WO2002077271A2 (en
Inventor
Dirk Vollenbroich
Christine Schramm
Original Assignee
Minerva Biolabs Gmbh
Dirk Vollenbroich
Christine Schramm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Minerva Biolabs Gmbh, Dirk Vollenbroich, Christine Schramm filed Critical Minerva Biolabs Gmbh
Priority to DE10291234T priority Critical patent/DE10291234D2/en
Publication of WO2002077271A2 publication Critical patent/WO2002077271A2/en
Publication of WO2002077271A3 publication Critical patent/WO2002077271A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Abstract

The invention relates to a method for detecting mycoplasma in biological material, comprising the following steps: a) the mycoplasma are lysed in the biological material by heat treatment, b) the released mycoplasma DNA is introduced into a PCR, which uses specific primers that however recognise a segment, common to the representatives of the Mollicutes class, of the gene that codes for the 16S rRNA of mycoplasma and either c) a conventional, non-real time PCR is carried out or the reaction feed material is processed in a device for the real time PCR. The invention also relates to the control plasmid, containing primers that recognise a segment of the gene that codes for the 16S rRNA of the mycoplasma genome, said segment being specific to the species of the micro-organism class Mollicutes, but common to the individual species of the Mycoplasma genus, in addition to the majority of representatives of the genera Ureaplasma, Spiroplasma, and Acholeplasma and to an amplicon, which is located between the primers and comprises the oligonucleotide sequence 5' -cgccctactggccacctgtccaga.
PCT/DE2002/001154 2001-03-25 2002-03-25 Control plasmid and method for detecting mycoplasma contamination in biological material WO2002077271A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE10291234T DE10291234D2 (en) 2001-03-25 2002-03-25 Control plasmid and method for the detection of mycoplasma contamination in biological material

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10115749.5 2001-03-25
DE10115749A DE10115749A1 (en) 2001-03-25 2001-03-25 Control plasmid and method for the detection of mycoplasma contamination in biological material

Publications (2)

Publication Number Publication Date
WO2002077271A2 WO2002077271A2 (en) 2002-10-03
WO2002077271A3 true WO2002077271A3 (en) 2003-08-21

Family

ID=7679665

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/001154 WO2002077271A2 (en) 2001-03-25 2002-03-25 Control plasmid and method for detecting mycoplasma contamination in biological material

Country Status (2)

Country Link
DE (2) DE10115749A1 (en)
WO (1) WO2002077271A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2742134A2 (en) 2011-08-11 2014-06-18 Qiagen GmbH Cell- or virus simulating means comprising encapsulated marker molecules
CN111235289B (en) * 2020-03-15 2022-04-22 中国农业科学院兰州兽医研究所 Specific PCR primer for detecting mycoplasma capricolum goat pneumonia subspecies

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5491062A (en) * 1993-11-23 1996-02-13 Stratagene Polynucleotide amplification mycoplasma assay, primers, and kits therefore
US5595871A (en) * 1993-08-25 1997-01-21 University Of Scranton Detection and prevention of mycoplasma hominis infection
US5654418A (en) * 1990-10-19 1997-08-05 Becton Dickinson And Company Nucleic acid probes useful for detecting microorganisms associated with vaginal infections
US5693467A (en) * 1995-05-19 1997-12-02 The American Type Culture Collection Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654418A (en) * 1990-10-19 1997-08-05 Becton Dickinson And Company Nucleic acid probes useful for detecting microorganisms associated with vaginal infections
US5595871A (en) * 1993-08-25 1997-01-21 University Of Scranton Detection and prevention of mycoplasma hominis infection
US5491062A (en) * 1993-11-23 1996-02-13 Stratagene Polynucleotide amplification mycoplasma assay, primers, and kits therefore
US5693467A (en) * 1995-05-19 1997-12-02 The American Type Culture Collection Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KUPPEVELD VAN F J M ET AL: "GENUS- AND SPECIES-SPECIFIC IDENTIFICATION OF MYCOPLASMAS BY 16S RRNA AMPLIFICATION", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 58, no. 8, August 1992 (1992-08-01), pages 2606 - 2615, XP000400754, ISSN: 0099-2240 *
OSSEWAARDE J.M. ET AL.,: "application of a mycoplasma group-specific PCR for monitoring decontamination of mycoplasma-infected chlamydia sp. strains", APP. ENVIROM. MICORBIOLOGY, vol. 62, no. 2, February 1996 (1996-02-01), pages 328 - 331, XP002241959 *
VOLLENBROICH D. ET AL.,: "Neue methoden zur bekämpfung von Mycoplasma Kontaminationen in Zell und viruskulturen", BIOSPEKTRUM, vol. 6, no. 3, 2000, pages 232 - 233, XP002241958 *

Also Published As

Publication number Publication date
DE10291234D2 (en) 2004-04-15
DE10115749A1 (en) 2002-10-10
WO2002077271A2 (en) 2002-10-03

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