WO2002074994A3 - Improved orestes sequencing method - Google Patents

Improved orestes sequencing method Download PDF

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Publication number
WO2002074994A3
WO2002074994A3 PCT/US2001/046665 US0146665W WO02074994A3 WO 2002074994 A3 WO2002074994 A3 WO 2002074994A3 US 0146665 W US0146665 W US 0146665W WO 02074994 A3 WO02074994 A3 WO 02074994A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
information
orestes
improved
Prior art date
Application number
PCT/US2001/046665
Other languages
French (fr)
Other versions
WO2002074994A2 (en
Inventor
Andrew John George Simpson
Emmanuel Dias-Neto
Ricardo R Brentani
Original Assignee
Ludwig Inst Cancer Res
Andrew John George Simpson
Emmanuel Dias-Neto
Ricardo R Brentani
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ludwig Inst Cancer Res, Andrew John George Simpson, Emmanuel Dias-Neto, Ricardo R Brentani filed Critical Ludwig Inst Cancer Res
Publication of WO2002074994A2 publication Critical patent/WO2002074994A2/en
Publication of WO2002074994A3 publication Critical patent/WO2002074994A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

The invention involves a method for obtaining nucleotide sequence information from nucleic acid molecules, such as cDNA. The method involves the use of arbitrary primers, and low stringency conditions. Rather than providing information from the termini of nucleic acid molecules, the method provides information on the more interesting and relevant internal portions of nucleic acid molecules. The method shows how to secure information on ORFs, and how to prepare contig sequences from any source.
PCT/US2001/046665 2000-11-07 2001-11-01 Improved orestes sequencing method WO2002074994A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24631300P 2000-11-07 2000-11-07
US60/246,313 2000-11-07

Publications (2)

Publication Number Publication Date
WO2002074994A2 WO2002074994A2 (en) 2002-09-26
WO2002074994A3 true WO2002074994A3 (en) 2003-08-14

Family

ID=22930138

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/046665 WO2002074994A2 (en) 2000-11-07 2001-11-01 Improved orestes sequencing method

Country Status (1)

Country Link
WO (1) WO2002074994A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5487985A (en) * 1990-10-15 1996-01-30 Stratagene Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
WO2000031299A2 (en) * 1998-11-20 2000-06-02 Ludwig Institute For Cancer Research Method for determining nucleotide sequences using arbitrary primers and low stringency
WO2001051518A2 (en) * 2000-01-14 2001-07-19 Ludwig Institute For Cancer Research Nucleic acids encoding human semaphorin proteins and use thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5487985A (en) * 1990-10-15 1996-01-30 Stratagene Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
WO2000031299A2 (en) * 1998-11-20 2000-06-02 Ludwig Institute For Cancer Research Method for determining nucleotide sequences using arbitrary primers and low stringency
WO2001051518A2 (en) * 2000-01-14 2001-07-19 Ludwig Institute For Cancer Research Nucleic acids encoding human semaphorin proteins and use thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DIAS NETO E ET AL: "Minilibraries constructed from cDNA generated by arbitrarily primed RT-PCR: an alternative to normalized libraries for the generation of ESTs from nanogram quantities of mRNA", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 186, 1997, pages 135 - 142, XP002138178, ISSN: 0378-1119 *
EMMANUEL DIAS NETO ET AL: "SHOTGUN SEQUENCING OF THE HUMAN TRANSCRIPTOME WITH ORF EXPRESSED SEQUENCE TAGS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 97, no. 7, 28 March 2000 (2000-03-28), pages 3491 - 3496, XP000996193, ISSN: 0027-8424 *
RADELOFF ET AL: "PRESELECTION OF SHOTGUN CLONES BY OLIGONUCLEOTIDE FINGERPRINTING: AN EFFICIENT AND HIGH THROUGHPUT STRATEGY TO REDUCE REDUNDANCY IN LARGE-SCALE SEQUENCING PROJECTS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 23, December 1998 (1998-12-01), pages 5358 - 5364, XP002103597, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2002074994A2 (en) 2002-09-26

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