WO2002063269A3 - Layered calibration standard for tissue sampling - Google Patents

Layered calibration standard for tissue sampling Download PDF

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Publication number
WO2002063269A3
WO2002063269A3 PCT/US2002/003281 US0203281W WO02063269A3 WO 2002063269 A3 WO2002063269 A3 WO 2002063269A3 US 0203281 W US0203281 W US 0203281W WO 02063269 A3 WO02063269 A3 WO 02063269A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorophore
calibration standard
tissue sampling
scattering layer
layered
Prior art date
Application number
PCT/US2002/003281
Other languages
French (fr)
Other versions
WO2002063269A2 (en
Inventor
William Apruzzese
Pierre Trepagnier
Russell Gray
James Mansfield
Christopher Lambert
Original Assignee
Argose Inc
William Apruzzese
Pierre Trepagnier
Russell Gray
James Mansfield
Christopher Lambert
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Argose Inc, William Apruzzese, Pierre Trepagnier, Russell Gray, James Mansfield, Christopher Lambert filed Critical Argose Inc
Priority to AU2002251877A priority Critical patent/AU2002251877A1/en
Publication of WO2002063269A2 publication Critical patent/WO2002063269A2/en
Publication of WO2002063269A3 publication Critical patent/WO2002063269A3/en

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274Calibration, base line adjustment, drift correction
    • G01N21/278Constitution of standards
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N2021/6417Spectrofluorimetric devices
    • G01N2021/6421Measuring at two or more wavelengths

Abstract

The invention relates to fluorescence calibration devices and methods that can mimic skin and other tissues. A calibration device (100) of the invention comprises at least one scattering layer (110), which is preferably non-fluorescent, and a second layer (120) containing one or more fluorophore (125). Light passes through the scattering layer (110) and excites the fluorophore (125). Light emitted from the fluorophore (125) passes back through the scattering layer (110) and into collecting optics, which can be measured and that measurement is used to correct for instrument drift.
PCT/US2002/003281 2001-02-06 2002-02-06 Layered calibration standard for tissue sampling WO2002063269A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002251877A AU2002251877A1 (en) 2001-02-06 2002-02-06 Layered calibration standard for tissue sampling

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26647001P 2001-02-06 2001-02-06
US60/266,470 2001-02-06

Publications (2)

Publication Number Publication Date
WO2002063269A2 WO2002063269A2 (en) 2002-08-15
WO2002063269A3 true WO2002063269A3 (en) 2004-07-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/003281 WO2002063269A2 (en) 2001-02-06 2002-02-06 Layered calibration standard for tissue sampling

Country Status (3)

Country Link
US (1) US20020133080A1 (en)
AU (1) AU2002251877A1 (en)
WO (1) WO2002063269A2 (en)

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