WO2002061428A8 - Method of disrupting interactions between biotin and biotin-binding compounds - Google Patents

Method of disrupting interactions between biotin and biotin-binding compounds

Info

Publication number
WO2002061428A8
WO2002061428A8 PCT/GB2002/000466 GB0200466W WO02061428A8 WO 2002061428 A8 WO2002061428 A8 WO 2002061428A8 GB 0200466 W GB0200466 W GB 0200466W WO 02061428 A8 WO02061428 A8 WO 02061428A8
Authority
WO
WIPO (PCT)
Prior art keywords
biotin
conjugate
compound
binding compounds
disrupted
Prior art date
Application number
PCT/GB2002/000466
Other languages
French (fr)
Other versions
WO2002061428A2 (en
WO2002061428A3 (en
Inventor
Anders Holmberg
Original Assignee
Magnetic Biosolutions Sweden A
Anders Holmberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Magnetic Biosolutions Sweden A, Anders Holmberg filed Critical Magnetic Biosolutions Sweden A
Priority to AU2002228214A priority Critical patent/AU2002228214B2/en
Priority to US10/470,846 priority patent/US7279285B2/en
Priority to AT02710163T priority patent/ATE553379T1/en
Priority to EP02710163A priority patent/EP1356290B1/en
Priority to CA2432538A priority patent/CA2432538C/en
Priority to JP2002561943A priority patent/JP4394879B2/en
Publication of WO2002061428A2 publication Critical patent/WO2002061428A2/en
Publication of WO2002061428A3 publication Critical patent/WO2002061428A3/en
Publication of WO2002061428A8 publication Critical patent/WO2002061428A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

The present invention relates to a method of disrupting a conjugate comprising a biotin compound and a biotin-binding compound, said method comprising the step of contacting said conjugate with an effective amount of a substantially aqueous solution under conditions such that the conjugate is disrupted, preferably reversibly disrupted, thereby forming a biotin compound and a biotin-binding compound. In particular, the invention relates to a method of reversibly releasing a biotinylated moiety from a streptavidin ( or avidin) coated support. Uses of the methods in techniques of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules are also encompassed by the present invention.
PCT/GB2002/000466 2001-02-01 2002-02-01 Method of disrupting interactions between biotin and biotin-binding compounds WO2002061428A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2002228214A AU2002228214B2 (en) 2001-02-01 2002-02-01 Method of disrupting interactions between biotin and biotin-binding compounds
US10/470,846 US7279285B2 (en) 2001-02-01 2002-02-01 Method of reversibly disrupting a conjugate comprising a biotin compound and a biotin-binding compound
AT02710163T ATE553379T1 (en) 2001-02-01 2002-02-01 METHOD FOR DESTROYING INTERACTIONS BETWEEN BIOTIN AND BIOTIN-BINDING COMPOUNDS
EP02710163A EP1356290B1 (en) 2001-02-01 2002-02-01 Method of disrupting interactions between biotin and biotin-binding compounds
CA2432538A CA2432538C (en) 2001-02-01 2002-02-01 Method of disrupting interactions between biotin and biotin-binding compounds
JP2002561943A JP4394879B2 (en) 2001-02-01 2002-02-01 Method for reversibly decomposing a complex containing a biotin compound and a compound that binds biotin

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0102568.3 2001-02-01
GBGB0102568.3A GB0102568D0 (en) 2001-02-01 2001-02-01 Method
US26652401P 2001-02-05 2001-02-05

Publications (3)

Publication Number Publication Date
WO2002061428A2 WO2002061428A2 (en) 2002-08-08
WO2002061428A3 WO2002061428A3 (en) 2003-04-17
WO2002061428A8 true WO2002061428A8 (en) 2003-11-27

Family

ID=9907949

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/000466 WO2002061428A2 (en) 2001-02-01 2002-02-01 Method of disrupting interactions between biotin and biotin-binding compounds

Country Status (8)

Country Link
US (1) US7279285B2 (en)
EP (1) EP1356290B1 (en)
JP (1) JP4394879B2 (en)
AT (1) ATE553379T1 (en)
AU (1) AU2002228214B2 (en)
CA (1) CA2432538C (en)
GB (1) GB0102568D0 (en)
WO (1) WO2002061428A2 (en)

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* Cited by examiner, † Cited by third party
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CA2515213A1 (en) * 2003-02-11 2004-08-26 University Of Washington Stimuli-responsive polymer conjugates and related methods
ATE418621T1 (en) 2003-04-24 2009-01-15 Afshin Ahmadian METHOD FOR DETECTING ALLEL-SPECIFIC MUTATIONS
EP2112168B1 (en) 2006-12-28 2014-10-01 Japan Tobacco Inc. Use of thermostable biotin-binding protein
US7981688B2 (en) 2007-03-08 2011-07-19 University Of Washington Stimuli-responsive magnetic nanoparticles and related methods
CN101896816B (en) * 2007-06-22 2014-08-20 B.R.A.H.M.S有限公司 Method for detecting analytes
DE102007044708A1 (en) 2007-09-18 2009-03-19 Eads Deutschland Gmbh Apparatus and method for regenerating biosensors
SG168174A1 (en) * 2008-07-16 2011-02-28 Kbi Biopharma Inc Methods and systems for manipulating particles using a fluidized bed
KR20100122366A (en) * 2009-05-12 2010-11-22 삼성전자주식회사 Magnetic nanoparticles for nucleic acid sequencing and the method using the same
US8426214B2 (en) * 2009-06-12 2013-04-23 University Of Washington System and method for magnetically concentrating and detecting biomarkers
US9080933B2 (en) 2009-11-09 2015-07-14 University Of Washington Through Its Center For Commercialization Stimuli-responsive polymer diagnostic assay comprising magnetic nanoparticles and capture conjugates
US20110117668A1 (en) * 2009-11-09 2011-05-19 University Of Washington Through Its Center For Commercialization Self-powered smart diagnostic devices
US20110318743A1 (en) * 2010-06-28 2011-12-29 Life Technologies Corporation Methods, workflows, kits, apparatuses, and computer program media for nucleic acid sample preparation for nucleic acid sequencing
EA027558B1 (en) 2011-05-19 2017-08-31 Эйджена Байосайенс, Инк. Process for multiplex nucleic acid identification
CN105917036B (en) 2013-08-19 2019-08-06 雅培分子公司 Next-generation sequencing library
US11225658B2 (en) * 2013-12-05 2022-01-18 New England Biolabs, Inc. Enrichment and sequencing of RNA species
EP3250928A1 (en) * 2015-01-26 2017-12-06 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Methods for a quantitative release of biotinylated peptides and proteins from streptavidin complexes
WO2016172579A1 (en) 2015-04-24 2016-10-27 Agena Bioscience, Inc, Multiplex methods for detection and quantification of minor variants
CN107406882B (en) 2015-04-24 2022-03-01 基纳生物技术有限公司 Multiplexing method for identification and quantification of minor alleles and polymorphisms
MA45489A (en) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh CELL CULTURE PROCESSES, ASSOCIATED KITS AND APPARATUS
JP7339160B2 (en) 2017-04-27 2023-09-05 ジュノ セラピューティクス ゲーエムベーハー Oligomeric particle reagents and methods of use thereof
US11905553B2 (en) 2018-01-29 2024-02-20 St. Jude Children's Research Hospital, Inc. Method for nucleic acid amplification
JPWO2020225971A1 (en) * 2019-05-08 2020-11-12
WO2020242856A1 (en) * 2019-05-31 2020-12-03 The Penn State Research Foundation Specific selection of immune cells using versatile display scaffolds
CN111676217B (en) * 2019-09-12 2021-10-26 南京君华基因科技有限公司 Method and system for improving nucleic acid yield and being compatible with subsequent reaction

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5387505A (en) 1990-05-04 1995-02-07 Eastman Kodak Company Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage
US5395856A (en) * 1991-05-16 1995-03-07 Rohm And Haas Company HPLC avidin monomer affinity resin
JPH0578369A (en) * 1991-08-12 1993-03-30 Mitsubishi Petrochem Co Ltd Method for separating and recovering d-biotin
IL114149A0 (en) * 1995-06-14 1995-10-31 Yeda Res & Dev Modified avidin and streptavidin molecules and use thereof
US6022688A (en) 1996-05-13 2000-02-08 Sequenom, Inc. Method for dissociating biotin complexes
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2001051067A1 (en) * 2000-01-13 2001-07-19 Baxter International Inc. Multiple inactivated blood factor anticoagulant composition

Also Published As

Publication number Publication date
JP2004527732A (en) 2004-09-09
CA2432538A1 (en) 2002-08-08
WO2002061428A2 (en) 2002-08-08
EP1356290B1 (en) 2012-04-11
CA2432538C (en) 2012-03-20
US7279285B2 (en) 2007-10-09
AU2002228214B2 (en) 2007-01-04
ATE553379T1 (en) 2012-04-15
JP4394879B2 (en) 2010-01-06
WO2002061428A3 (en) 2003-04-17
EP1356290A2 (en) 2003-10-29
US20040077024A1 (en) 2004-04-22
GB0102568D0 (en) 2001-03-21

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